Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_09390 and IAI47_09395 are separated by 163 nucleotides IAI47_09395 and IAI47_09400 are separated by 35 nucleotides IAI47_09400 and IAI47_09405 overlap by 4 nucleotides IAI47_09405 and IAI47_09410 are separated by 54 nucleotides IAI47_09410 and IAI47_09415 overlap by 20 nucleotides
IAI47_09390: IAI47_09390 - phage shock protein operon transcriptional activator, at 1,984,454 to 1,985,455
_09390
IAI47_09395: IAI47_09395 - phage shock protein PspA, at 1,985,619 to 1,986,284
_09395
IAI47_09400: IAI47_09400 - envelope stress response membrane protein PspB, at 1,986,320 to 1,986,547
_09400
IAI47_09405: IAI47_09405 - envelope stress response membrane protein PspC, at 1,986,544 to 1,986,894
_09405
IAI47_09410: IAI47_09410 - phage shock protein PspD, at 1,986,949 to 1,987,197
_09410
IAI47_09415: IAI47_09415 - YcjX family protein, at 1,987,178 to 1,988,575
_09415
Position (kb)
1985
1986
1987 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1984.637 kb on - strand, within IAI47_09390 at 1984.637 kb on - strand, within IAI47_09390 at 1984.702 kb on + strand, within IAI47_09390 at 1984.702 kb on + strand, within IAI47_09390 at 1984.703 kb on - strand, within IAI47_09390 at 1984.703 kb on - strand, within IAI47_09390 at 1984.703 kb on - strand, within IAI47_09390 at 1984.735 kb on + strand, within IAI47_09390 at 1984.735 kb on + strand, within IAI47_09390 at 1984.736 kb on - strand, within IAI47_09390 at 1984.736 kb on - strand, within IAI47_09390 at 1984.736 kb on - strand, within IAI47_09390 at 1984.736 kb on - strand, within IAI47_09390 at 1984.736 kb on - strand, within IAI47_09390 at 1984.743 kb on + strand, within IAI47_09390 at 1984.743 kb on + strand, within IAI47_09390 at 1984.768 kb on + strand, within IAI47_09390 at 1984.768 kb on + strand, within IAI47_09390 at 1984.768 kb on + strand, within IAI47_09390 at 1984.768 kb on + strand, within IAI47_09390 at 1984.768 kb on + strand, within IAI47_09390 at 1984.769 kb on - strand, within IAI47_09390 at 1984.769 kb on - strand, within IAI47_09390 at 1984.769 kb on - strand, within IAI47_09390 at 1984.769 kb on - strand, within IAI47_09390 at 1984.769 kb on - strand, within IAI47_09390 at 1984.769 kb on - strand, within IAI47_09390 at 1984.797 kb on + strand, within IAI47_09390 at 1984.798 kb on - strand, within IAI47_09390 at 1984.798 kb on - strand, within IAI47_09390 at 1984.798 kb on - strand, within IAI47_09390 at 1984.829 kb on - strand, within IAI47_09390 at 1984.845 kb on - strand, within IAI47_09390 at 1985.012 kb on - strand, within IAI47_09390 at 1985.079 kb on + strand, within IAI47_09390 at 1985.079 kb on + strand, within IAI47_09390 at 1985.080 kb on - strand, within IAI47_09390 at 1985.080 kb on - strand, within IAI47_09390 at 1985.087 kb on + strand, within IAI47_09390 at 1985.088 kb on - strand, within IAI47_09390 at 1985.276 kb on - strand, within IAI47_09390 at 1985.304 kb on + strand, within IAI47_09390 at 1985.305 kb on - strand, within IAI47_09390 at 1985.305 kb on - strand, within IAI47_09390 at 1985.430 kb on - strand at 1985.443 kb on + strand at 1985.567 kb on + strand at 1985.567 kb on + strand at 1985.568 kb on - strand at 1985.571 kb on - strand at 1985.593 kb on + strand at 1985.602 kb on - strand at 1985.650 kb on + strand at 1985.650 kb on + strand at 1985.650 kb on + strand at 1985.663 kb on + strand at 1985.663 kb on + strand at 1985.737 kb on + strand, within IAI47_09395 at 1985.737 kb on + strand, within IAI47_09395 at 1985.798 kb on + strand, within IAI47_09395 at 1985.969 kb on + strand, within IAI47_09395 at 1986.095 kb on + strand, within IAI47_09395 at 1986.095 kb on + strand, within IAI47_09395 at 1986.209 kb on + strand, within IAI47_09395 at 1986.210 kb on - strand, within IAI47_09395 at 1986.248 kb on + strand at 1986.248 kb on + strand at 1986.300 kb on + strand at 1986.300 kb on + strand at 1986.300 kb on + strand at 1986.300 kb on + strand at 1986.300 kb on + strand at 1986.301 kb on - strand at 1986.301 kb on - strand at 1986.342 kb on + strand at 1986.342 kb on - strand at 1986.343 kb on - strand, within IAI47_09400 at 1986.343 kb on - strand, within IAI47_09400 at 1986.395 kb on + strand, within IAI47_09400 at 1986.395 kb on + strand, within IAI47_09400 at 1986.395 kb on + strand, within IAI47_09400 at 1986.396 kb on - strand, within IAI47_09400 at 1986.396 kb on - strand, within IAI47_09400 at 1986.396 kb on - strand, within IAI47_09400 at 1986.461 kb on - strand, within IAI47_09400 at 1986.491 kb on - strand, within IAI47_09400 at 1986.491 kb on - strand, within IAI47_09400 at 1986.529 kb on + strand at 1986.529 kb on + strand at 1986.559 kb on - strand at 1986.559 kb on - strand at 1986.619 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.662 kb on + strand, within IAI47_09405 at 1986.663 kb on - strand, within IAI47_09405 at 1986.667 kb on + strand, within IAI47_09405 at 1986.668 kb on + strand, within IAI47_09405 at 1986.668 kb on + strand, within IAI47_09405 at 1986.668 kb on + strand, within IAI47_09405 at 1986.684 kb on + strand, within IAI47_09405 at 1986.684 kb on + strand, within IAI47_09405 at 1986.690 kb on + strand, within IAI47_09405 at 1986.691 kb on - strand, within IAI47_09405 at 1986.704 kb on - strand, within IAI47_09405 at 1986.704 kb on - strand, within IAI47_09405 at 1986.844 kb on + strand, within IAI47_09405 at 1986.844 kb on + strand, within IAI47_09405 at 1986.845 kb on - strand, within IAI47_09405 at 1986.845 kb on - strand, within IAI47_09405 at 1986.896 kb on + strand at 1986.897 kb on - strand at 1986.898 kb on + strand at 1986.898 kb on + strand at 1986.898 kb on + strand at 1986.898 kb on + strand at 1986.899 kb on - strand at 1986.899 kb on - strand at 1986.899 kb on - strand at 1986.899 kb on - strand at 1986.899 kb on - strand at 1986.899 kb on - strand at 1986.901 kb on + strand at 1986.902 kb on - strand at 1986.902 kb on - strand at 1987.017 kb on + strand, within IAI47_09410 at 1987.018 kb on - strand, within IAI47_09410 at 1987.023 kb on + strand, within IAI47_09410 at 1987.035 kb on + strand, within IAI47_09410 at 1987.035 kb on + strand, within IAI47_09410 at 1987.036 kb on - strand, within IAI47_09410 at 1987.039 kb on + strand, within IAI47_09410 at 1987.039 kb on + strand, within IAI47_09410 at 1987.040 kb on - strand, within IAI47_09410 at 1987.040 kb on - strand, within IAI47_09410 at 1987.094 kb on + strand, within IAI47_09410 at 1987.200 kb on + strand at 1987.200 kb on + strand at 1987.201 kb on - strand at 1987.209 kb on + strand at 1987.258 kb on - strand at 1987.282 kb on + strand at 1987.283 kb on - strand at 1987.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,984,637 - IAI47_09390 0.18 +0.2 1,984,637 - IAI47_09390 0.18 +0.4 1,984,702 + IAI47_09390 0.25 -2.0 1,984,702 + IAI47_09390 0.25 -1.3 1,984,703 - IAI47_09390 0.25 -2.2 1,984,703 - IAI47_09390 0.25 +1.3 1,984,703 - IAI47_09390 0.25 +1.2 1,984,735 + IAI47_09390 0.28 -0.2 1,984,735 + IAI47_09390 0.28 +1.4 1,984,736 - IAI47_09390 0.28 +0.7 1,984,736 - IAI47_09390 0.28 +1.9 1,984,736 - IAI47_09390 0.28 -1.8 1,984,736 - IAI47_09390 0.28 +1.7 1,984,736 - IAI47_09390 0.28 +0.4 1,984,743 + IAI47_09390 0.29 -2.2 1,984,743 + IAI47_09390 0.29 +1.3 1,984,768 + IAI47_09390 0.31 +2.0 1,984,768 + IAI47_09390 0.31 -0.3 1,984,768 + IAI47_09390 0.31 -0.8 1,984,768 + IAI47_09390 0.31 -2.1 1,984,768 + IAI47_09390 0.31 +1.2 1,984,769 - IAI47_09390 0.31 -1.4 1,984,769 - IAI47_09390 0.31 +1.9 1,984,769 - IAI47_09390 0.31 +2.2 1,984,769 - IAI47_09390 0.31 +0.7 1,984,769 - IAI47_09390 0.31 +0.5 1,984,769 - IAI47_09390 0.31 +0.6 1,984,797 + IAI47_09390 0.34 -1.1 1,984,798 - IAI47_09390 0.34 -0.5 1,984,798 - IAI47_09390 0.34 -1.4 1,984,798 - IAI47_09390 0.34 +1.3 1,984,829 - IAI47_09390 0.37 -0.8 1,984,845 - IAI47_09390 0.39 -1.7 1,985,012 - IAI47_09390 0.56 +2.7 1,985,079 + IAI47_09390 0.62 -0.8 1,985,079 + IAI47_09390 0.62 +1.3 1,985,080 - IAI47_09390 0.62 +2.7 1,985,080 - IAI47_09390 0.62 -1.3 1,985,087 + IAI47_09390 0.63 +0.6 1,985,088 - IAI47_09390 0.63 -0.7 1,985,276 - IAI47_09390 0.82 -0.7 1,985,304 + IAI47_09390 0.85 -0.9 1,985,305 - IAI47_09390 0.85 +0.2 1,985,305 - IAI47_09390 0.85 +0.5 1,985,430 - -1.0 1,985,443 + +1.2 1,985,567 + -0.9 1,985,567 + +1.5 1,985,568 - -2.3 1,985,571 - -1.6 1,985,593 + +2.4 1,985,602 - -0.5 1,985,650 + -1.8 1,985,650 + -3.4 1,985,650 + +1.4 1,985,663 + -0.1 1,985,663 + +0.6 1,985,737 + IAI47_09395 0.18 -2.2 1,985,737 + IAI47_09395 0.18 -0.4 1,985,798 + IAI47_09395 0.27 -1.6 1,985,969 + IAI47_09395 0.53 -1.8 1,986,095 + IAI47_09395 0.71 +0.2 1,986,095 + IAI47_09395 0.71 -0.3 1,986,209 + IAI47_09395 0.89 -1.1 1,986,210 - IAI47_09395 0.89 -1.4 1,986,248 + +1.0 1,986,248 + +0.1 1,986,300 + -1.4 1,986,300 + -0.4 1,986,300 + +1.3 1,986,300 + +1.3 1,986,300 + -2.8 1,986,301 - +0.9 1,986,301 - +2.3 1,986,342 + +2.7 1,986,342 - +0.6 1,986,343 - IAI47_09400 0.10 -1.1 1,986,343 - IAI47_09400 0.10 +2.4 1,986,395 + IAI47_09400 0.33 -0.4 1,986,395 + IAI47_09400 0.33 -2.1 1,986,395 + IAI47_09400 0.33 +0.2 1,986,396 - IAI47_09400 0.33 -1.2 1,986,396 - IAI47_09400 0.33 +1.6 1,986,396 - IAI47_09400 0.33 -0.2 1,986,461 - IAI47_09400 0.62 -1.3 1,986,491 - IAI47_09400 0.75 -0.3 1,986,491 - IAI47_09400 0.75 +1.4 1,986,529 + -0.2 1,986,529 + +0.7 1,986,559 - +0.8 1,986,559 - +1.5 1,986,619 + IAI47_09405 0.21 +0.8 1,986,662 + IAI47_09405 0.34 +0.4 1,986,662 + IAI47_09405 0.34 +0.0 1,986,662 + IAI47_09405 0.34 -2.5 1,986,662 + IAI47_09405 0.34 -1.0 1,986,662 + IAI47_09405 0.34 -1.3 1,986,662 + IAI47_09405 0.34 -0.3 1,986,662 + IAI47_09405 0.34 -1.9 1,986,663 - IAI47_09405 0.34 +1.3 1,986,667 + IAI47_09405 0.35 +0.2 1,986,668 + IAI47_09405 0.35 +4.4 1,986,668 + IAI47_09405 0.35 +0.4 1,986,668 + IAI47_09405 0.35 +0.2 1,986,684 + IAI47_09405 0.40 -0.5 1,986,684 + IAI47_09405 0.40 -2.8 1,986,690 + IAI47_09405 0.42 -0.6 1,986,691 - IAI47_09405 0.42 +0.8 1,986,704 - IAI47_09405 0.46 -2.8 1,986,704 - IAI47_09405 0.46 -0.5 1,986,844 + IAI47_09405 0.85 -0.4 1,986,844 + IAI47_09405 0.85 +0.6 1,986,845 - IAI47_09405 0.86 -0.5 1,986,845 - IAI47_09405 0.86 -1.5 1,986,896 + +1.3 1,986,897 - +1.7 1,986,898 + +0.9 1,986,898 + -3.3 1,986,898 + -1.5 1,986,898 + +2.7 1,986,899 - +0.5 1,986,899 - +2.0 1,986,899 - -2.8 1,986,899 - +0.0 1,986,899 - +0.4 1,986,899 - -1.7 1,986,901 + -0.7 1,986,902 - +0.0 1,986,902 - +0.9 1,987,017 + IAI47_09410 0.27 +1.2 1,987,018 - IAI47_09410 0.28 -0.1 1,987,023 + IAI47_09410 0.30 -3.2 1,987,035 + IAI47_09410 0.35 -1.3 1,987,035 + IAI47_09410 0.35 +1.9 1,987,036 - IAI47_09410 0.35 -2.3 1,987,039 + IAI47_09410 0.36 -0.3 1,987,039 + IAI47_09410 0.36 -0.7 1,987,040 - IAI47_09410 0.37 -0.9 1,987,040 - IAI47_09410 0.37 +0.6 1,987,094 + IAI47_09410 0.58 +3.0 1,987,200 + +1.1 1,987,200 + -1.9 1,987,201 - +0.3 1,987,209 + -2.0 1,987,258 - -2.0 1,987,282 + +1.2 1,987,283 - +0.9 1,987,283 - -1.7
Or see this region's nucleotide sequence