Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01540 and IAI47_01545 are separated by 5 nucleotides IAI47_01545 and IAI47_01550 overlap by 11 nucleotides IAI47_01550 and IAI47_01555 are separated by 239 nucleotides
IAI47_01540: IAI47_01540 - signal recognition particle-docking protein FtsY, at 334,664 to 336,364
_01540
IAI47_01545: IAI47_01545 - cell division ATP-binding protein FtsE, at 336,370 to 337,038
_01545
IAI47_01550: IAI47_01550 - cell division protein FtsX, at 337,028 to 338,011
_01550
IAI47_01555: IAI47_01555 - RNA polymerase sigma factor RpoH, at 338,251 to 339,108
_01555
Position (kb) 337
338
339 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 336.362 kb on + strand at 336.362 kb on + strand at 336.362 kb on + strand at 336.362 kb on + strand at 336.362 kb on + strand at 336.362 kb on + strand at 336.363 kb on - strand at 336.363 kb on - strand at 336.363 kb on - strand at 336.367 kb on - strand at 336.367 kb on - strand at 336.367 kb on - strand at 336.393 kb on + strand at 336.400 kb on + strand at 336.400 kb on + strand at 336.401 kb on - strand at 336.401 kb on - strand at 336.401 kb on - strand at 336.490 kb on - strand, within IAI47_01545 at 336.496 kb on - strand, within IAI47_01545 at 336.496 kb on - strand, within IAI47_01545 at 336.496 kb on - strand, within IAI47_01545 at 336.496 kb on - strand, within IAI47_01545 at 336.685 kb on - strand, within IAI47_01545 at 336.708 kb on + strand, within IAI47_01545 at 336.708 kb on + strand, within IAI47_01545 at 336.708 kb on + strand, within IAI47_01545 at 336.709 kb on - strand, within IAI47_01545 at 336.709 kb on - strand, within IAI47_01545 at 336.738 kb on + strand, within IAI47_01545 at 336.739 kb on - strand, within IAI47_01545 at 336.770 kb on - strand, within IAI47_01545 at 336.918 kb on + strand, within IAI47_01545 at 336.978 kb on + strand at 336.978 kb on + strand at 336.978 kb on + strand at 336.978 kb on + strand at 336.979 kb on - strand at 336.979 kb on - strand at 336.979 kb on - strand at 336.983 kb on - strand at 337.036 kb on + strand at 337.037 kb on - strand at 337.046 kb on - strand at 337.046 kb on - strand at 337.087 kb on + strand at 337.088 kb on - strand at 337.088 kb on - strand at 337.088 kb on - strand at 337.088 kb on - strand at 337.125 kb on - strand at 337.192 kb on + strand, within IAI47_01550 at 337.192 kb on + strand, within IAI47_01550 at 337.192 kb on + strand, within IAI47_01550 at 337.192 kb on + strand, within IAI47_01550 at 337.226 kb on + strand, within IAI47_01550 at 337.226 kb on + strand, within IAI47_01550 at 337.226 kb on + strand, within IAI47_01550 at 337.227 kb on - strand, within IAI47_01550 at 337.227 kb on - strand, within IAI47_01550 at 337.227 kb on - strand, within IAI47_01550 at 337.268 kb on + strand, within IAI47_01550 at 337.284 kb on + strand, within IAI47_01550 at 337.284 kb on + strand, within IAI47_01550 at 337.285 kb on - strand, within IAI47_01550 at 337.285 kb on - strand, within IAI47_01550 at 337.292 kb on + strand, within IAI47_01550 at 337.292 kb on + strand, within IAI47_01550 at 337.292 kb on + strand, within IAI47_01550 at 337.292 kb on + strand, within IAI47_01550 at 337.293 kb on - strand, within IAI47_01550 at 337.293 kb on - strand, within IAI47_01550 at 337.372 kb on + strand, within IAI47_01550 at 337.372 kb on + strand, within IAI47_01550 at 337.372 kb on + strand, within IAI47_01550 at 337.372 kb on + strand, within IAI47_01550 at 337.372 kb on + strand, within IAI47_01550 at 337.372 kb on + strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.373 kb on - strand, within IAI47_01550 at 337.376 kb on + strand, within IAI47_01550 at 337.376 kb on + strand, within IAI47_01550 at 337.376 kb on + strand, within IAI47_01550 at 337.377 kb on - strand, within IAI47_01550 at 337.377 kb on - strand, within IAI47_01550 at 337.441 kb on + strand, within IAI47_01550 at 337.442 kb on - strand, within IAI47_01550 at 337.480 kb on + strand, within IAI47_01550 at 337.481 kb on - strand, within IAI47_01550 at 337.675 kb on + strand, within IAI47_01550 at 337.676 kb on - strand, within IAI47_01550 at 337.717 kb on + strand, within IAI47_01550 at 337.718 kb on - strand, within IAI47_01550 at 337.874 kb on + strand, within IAI47_01550 at 337.875 kb on + strand, within IAI47_01550 at 337.875 kb on + strand, within IAI47_01550 at 337.875 kb on + strand, within IAI47_01550 at 337.875 kb on + strand, within IAI47_01550 at 337.875 kb on + strand, within IAI47_01550 at 337.876 kb on - strand, within IAI47_01550 at 337.876 kb on - strand, within IAI47_01550 at 337.876 kb on - strand, within IAI47_01550 at 337.876 kb on - strand, within IAI47_01550 at 337.882 kb on + strand, within IAI47_01550 at 337.882 kb on + strand, within IAI47_01550 at 337.883 kb on - strand, within IAI47_01550 at 337.933 kb on - strand at 337.981 kb on - strand at 337.989 kb on + strand at 337.989 kb on + strand at 337.990 kb on - strand at 337.990 kb on - strand at 337.990 kb on - strand at 338.000 kb on - strand at 338.000 kb on - strand at 338.010 kb on - strand at 338.033 kb on + strand at 338.033 kb on + strand at 338.035 kb on + strand at 338.035 kb on + strand at 338.035 kb on + strand at 338.035 kb on + strand at 338.036 kb on - strand at 338.036 kb on - strand at 338.036 kb on - strand at 338.036 kb on - strand at 338.036 kb on - strand at 338.036 kb on - strand at 338.143 kb on - strand at 338.150 kb on + strand at 338.150 kb on + strand at 338.150 kb on + strand at 338.150 kb on + strand at 338.150 kb on + strand at 338.150 kb on + strand at 338.151 kb on - strand at 338.151 kb on - strand at 338.151 kb on - strand at 338.151 kb on - strand at 338.151 kb on - strand at 338.155 kb on - strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.192 kb on + strand at 338.197 kb on + strand at 338.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 336,362 + -0.1 336,362 + -0.8 336,362 + -1.4 336,362 + +0.5 336,362 + -2.2 336,362 + +0.1 336,363 - -1.2 336,363 - -2.3 336,363 - -1.9 336,367 - -2.3 336,367 - -0.1 336,367 - -2.0 336,393 + +1.4 336,400 + +0.8 336,400 + -1.2 336,401 - -0.5 336,401 - -0.1 336,401 - -1.2 336,490 - IAI47_01545 0.18 +0.4 336,496 - IAI47_01545 0.19 -2.7 336,496 - IAI47_01545 0.19 -1.8 336,496 - IAI47_01545 0.19 -1.5 336,496 - IAI47_01545 0.19 -3.5 336,685 - IAI47_01545 0.47 -0.7 336,708 + IAI47_01545 0.51 -1.8 336,708 + IAI47_01545 0.51 +0.4 336,708 + IAI47_01545 0.51 -1.9 336,709 - IAI47_01545 0.51 -0.1 336,709 - IAI47_01545 0.51 +0.4 336,738 + IAI47_01545 0.55 -0.8 336,739 - IAI47_01545 0.55 -2.0 336,770 - IAI47_01545 0.60 -2.2 336,918 + IAI47_01545 0.82 -2.4 336,978 + -0.9 336,978 + +1.4 336,978 + +0.7 336,978 + -2.3 336,979 - -0.2 336,979 - -0.5 336,979 - +0.8 336,983 - +0.9 337,036 + +1.3 337,037 - +0.4 337,046 - -1.7 337,046 - -0.8 337,087 + -3.3 337,088 - -0.6 337,088 - -1.1 337,088 - +0.6 337,088 - -1.6 337,125 - +1.8 337,192 + IAI47_01550 0.17 +0.3 337,192 + IAI47_01550 0.17 +0.0 337,192 + IAI47_01550 0.17 -0.1 337,192 + IAI47_01550 0.17 -2.5 337,226 + IAI47_01550 0.20 +0.5 337,226 + IAI47_01550 0.20 -0.6 337,226 + IAI47_01550 0.20 +0.9 337,227 - IAI47_01550 0.20 -1.6 337,227 - IAI47_01550 0.20 -2.3 337,227 - IAI47_01550 0.20 -0.2 337,268 + IAI47_01550 0.24 +0.3 337,284 + IAI47_01550 0.26 -2.8 337,284 + IAI47_01550 0.26 -2.2 337,285 - IAI47_01550 0.26 -0.7 337,285 - IAI47_01550 0.26 -2.2 337,292 + IAI47_01550 0.27 -0.0 337,292 + IAI47_01550 0.27 -1.0 337,292 + IAI47_01550 0.27 -0.8 337,292 + IAI47_01550 0.27 +0.7 337,293 - IAI47_01550 0.27 -1.0 337,293 - IAI47_01550 0.27 -1.8 337,372 + IAI47_01550 0.35 -1.3 337,372 + IAI47_01550 0.35 +0.9 337,372 + IAI47_01550 0.35 -2.3 337,372 + IAI47_01550 0.35 -0.2 337,372 + IAI47_01550 0.35 -1.4 337,372 + IAI47_01550 0.35 -1.9 337,373 - IAI47_01550 0.35 +2.7 337,373 - IAI47_01550 0.35 -3.2 337,373 - IAI47_01550 0.35 -0.6 337,373 - IAI47_01550 0.35 -1.0 337,373 - IAI47_01550 0.35 -0.6 337,373 - IAI47_01550 0.35 -0.8 337,373 - IAI47_01550 0.35 +3.1 337,376 + IAI47_01550 0.35 -1.4 337,376 + IAI47_01550 0.35 -2.7 337,376 + IAI47_01550 0.35 -2.2 337,377 - IAI47_01550 0.35 +2.3 337,377 - IAI47_01550 0.35 -0.2 337,441 + IAI47_01550 0.42 -2.4 337,442 - IAI47_01550 0.42 -2.2 337,480 + IAI47_01550 0.46 -2.2 337,481 - IAI47_01550 0.46 -1.9 337,675 + IAI47_01550 0.66 +0.8 337,676 - IAI47_01550 0.66 +1.0 337,717 + IAI47_01550 0.70 -0.5 337,718 - IAI47_01550 0.70 +2.0 337,874 + IAI47_01550 0.86 +0.1 337,875 + IAI47_01550 0.86 -1.2 337,875 + IAI47_01550 0.86 +0.1 337,875 + IAI47_01550 0.86 +1.2 337,875 + IAI47_01550 0.86 +0.4 337,875 + IAI47_01550 0.86 +0.8 337,876 - IAI47_01550 0.86 -2.2 337,876 - IAI47_01550 0.86 +1.0 337,876 - IAI47_01550 0.86 -2.4 337,876 - IAI47_01550 0.86 -2.2 337,882 + IAI47_01550 0.87 +1.0 337,882 + IAI47_01550 0.87 -0.5 337,883 - IAI47_01550 0.87 -1.8 337,933 - -1.3 337,981 - -1.4 337,989 + +0.8 337,989 + +3.4 337,990 - -3.2 337,990 - +0.8 337,990 - -2.9 338,000 - -2.2 338,000 - -1.5 338,010 - -0.9 338,033 + +0.3 338,033 + -0.7 338,035 + -1.2 338,035 + -0.2 338,035 + -0.0 338,035 + -0.7 338,036 - +1.7 338,036 - +1.1 338,036 - +0.8 338,036 - -1.2 338,036 - +0.0 338,036 - +0.5 338,143 - -0.1 338,150 + -0.4 338,150 + -1.5 338,150 + +1.2 338,150 + +1.3 338,150 + -0.8 338,150 + -0.4 338,151 - -2.4 338,151 - +0.1 338,151 - +1.9 338,151 - +0.7 338,151 - -1.6 338,155 - -0.5 338,192 + -1.2 338,192 + +1.5 338,192 + -0.6 338,192 + +2.9 338,192 + +1.0 338,192 + +0.8 338,192 + -0.8 338,192 + -0.4 338,192 + +0.7 338,192 + -3.0 338,197 + +2.2 338,197 + +1.2
Or see this region's nucleotide sequence