Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01350 and IAI47_01355 are separated by 56 nucleotides IAI47_01355 and IAI47_01360 are separated by 414 nucleotides IAI47_01360 and IAI47_01365 are separated by 37 nucleotides
IAI47_01350: IAI47_01350 - 16S rRNA (guanine(1516)-N(2))-methyltransferase RsmJ, at 292,244 to 292,990
_01350
IAI47_01355: IAI47_01355 - universal stress protein UspA, at 293,047 to 293,484
_01355
IAI47_01360: IAI47_01360 - universal stress protein UspB, at 293,899 to 294,234
_01360
IAI47_01365: IAI47_01365 - inorganic phosphate transporter PitA, at 294,272 to 295,774
_01365
Position (kb) 293
294
295 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 292.907 kb on + strand, within IAI47_01350 at 292.907 kb on + strand, within IAI47_01350 at 292.907 kb on + strand, within IAI47_01350 at 292.908 kb on - strand, within IAI47_01350 at 292.908 kb on - strand, within IAI47_01350 at 292.908 kb on - strand, within IAI47_01350 at 292.908 kb on - strand, within IAI47_01350 at 292.908 kb on - strand, within IAI47_01350 at 292.972 kb on + strand at 292.972 kb on + strand at 292.972 kb on + strand at 292.972 kb on + strand at 292.972 kb on + strand at 292.973 kb on - strand at 292.973 kb on - strand at 292.973 kb on - strand at 292.973 kb on - strand at 292.973 kb on - strand at 292.973 kb on - strand at 292.976 kb on + strand at 292.976 kb on + strand at 292.976 kb on + strand at 292.976 kb on + strand at 292.976 kb on + strand at 292.976 kb on + strand at 292.976 kb on + strand at 292.977 kb on - strand at 292.977 kb on - strand at 292.977 kb on - strand at 292.995 kb on - strand at 292.996 kb on + strand at 293.023 kb on + strand at 293.023 kb on + strand at 293.024 kb on - strand at 293.091 kb on + strand, within IAI47_01355 at 293.100 kb on + strand, within IAI47_01355 at 293.100 kb on + strand, within IAI47_01355 at 293.100 kb on + strand, within IAI47_01355 at 293.101 kb on - strand, within IAI47_01355 at 293.180 kb on + strand, within IAI47_01355 at 293.180 kb on + strand, within IAI47_01355 at 293.180 kb on + strand, within IAI47_01355 at 293.180 kb on + strand, within IAI47_01355 at 293.180 kb on + strand, within IAI47_01355 at 293.181 kb on - strand, within IAI47_01355 at 293.181 kb on - strand, within IAI47_01355 at 293.181 kb on - strand, within IAI47_01355 at 293.301 kb on + strand, within IAI47_01355 at 293.301 kb on + strand, within IAI47_01355 at 293.302 kb on - strand, within IAI47_01355 at 293.313 kb on + strand, within IAI47_01355 at 293.313 kb on + strand, within IAI47_01355 at 293.313 kb on + strand, within IAI47_01355 at 293.313 kb on + strand, within IAI47_01355 at 293.313 kb on + strand, within IAI47_01355 at 293.314 kb on - strand, within IAI47_01355 at 293.320 kb on + strand, within IAI47_01355 at 293.345 kb on + strand, within IAI47_01355 at 293.358 kb on - strand, within IAI47_01355 at 293.358 kb on - strand, within IAI47_01355 at 293.397 kb on - strand, within IAI47_01355 at 293.397 kb on - strand, within IAI47_01355 at 293.455 kb on + strand at 293.455 kb on + strand at 293.455 kb on + strand at 293.456 kb on - strand at 293.456 kb on - strand at 293.475 kb on + strand at 293.475 kb on + strand at 293.475 kb on + strand at 293.476 kb on - strand at 293.476 kb on - strand at 293.476 kb on - strand at 293.476 kb on - strand at 293.476 kb on - strand at 293.476 kb on - strand at 293.477 kb on + strand at 293.477 kb on + strand at 293.477 kb on + strand at 293.478 kb on - strand at 293.478 kb on - strand at 293.478 kb on - strand at 293.478 kb on - strand at 293.478 kb on - strand at 293.478 kb on - strand at 293.487 kb on + strand at 293.487 kb on + strand at 293.487 kb on + strand at 293.488 kb on - strand at 293.508 kb on - strand at 293.544 kb on + strand at 293.545 kb on - strand at 293.618 kb on + strand at 293.618 kb on + strand at 293.619 kb on - strand at 293.620 kb on + strand at 293.620 kb on + strand at 293.620 kb on + strand at 293.620 kb on + strand at 293.621 kb on - strand at 293.621 kb on - strand at 293.629 kb on + strand at 293.629 kb on + strand at 293.630 kb on - strand at 293.630 kb on - strand at 293.630 kb on - strand at 293.631 kb on + strand at 293.631 kb on + strand at 293.632 kb on - strand at 293.632 kb on - strand at 293.632 kb on - strand at 293.632 kb on - strand at 293.632 kb on - strand at 293.647 kb on + strand at 293.647 kb on + strand at 293.673 kb on - strand at 293.678 kb on + strand at 293.678 kb on + strand at 293.678 kb on + strand at 293.679 kb on - strand at 293.679 kb on - strand at 293.679 kb on - strand at 293.679 kb on - strand at 293.679 kb on - strand at 293.691 kb on - strand at 293.702 kb on - strand at 293.702 kb on - strand at 293.702 kb on - strand at 293.751 kb on + strand at 293.751 kb on + strand at 293.752 kb on - strand at 293.898 kb on + strand at 293.898 kb on + strand at 293.898 kb on + strand at 293.898 kb on + strand at 293.898 kb on + strand at 293.898 kb on + strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.899 kb on - strand at 293.910 kb on + strand at 293.910 kb on + strand at 293.911 kb on - strand at 293.911 kb on - strand at 293.943 kb on + strand, within IAI47_01360 at 293.943 kb on + strand, within IAI47_01360 at 293.943 kb on + strand, within IAI47_01360 at 293.943 kb on + strand, within IAI47_01360 at 293.944 kb on - strand, within IAI47_01360 at 293.944 kb on - strand, within IAI47_01360 at 293.944 kb on - strand, within IAI47_01360 at 293.944 kb on - strand, within IAI47_01360 at 293.944 kb on - strand, within IAI47_01360 at 293.952 kb on + strand, within IAI47_01360 at 293.952 kb on + strand, within IAI47_01360 at 293.952 kb on + strand, within IAI47_01360 at 293.984 kb on + strand, within IAI47_01360 at 293.984 kb on + strand, within IAI47_01360 at 293.993 kb on + strand, within IAI47_01360 at 293.997 kb on - strand, within IAI47_01360 at 294.019 kb on + strand, within IAI47_01360 at 294.019 kb on + strand, within IAI47_01360 at 294.019 kb on + strand, within IAI47_01360 at 294.020 kb on - strand, within IAI47_01360 at 294.020 kb on - strand, within IAI47_01360 at 294.020 kb on - strand, within IAI47_01360 at 294.020 kb on - strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.025 kb on + strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.026 kb on - strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.091 kb on + strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.092 kb on - strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.093 kb on + strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.094 kb on - strand, within IAI47_01360 at 294.135 kb on + strand, within IAI47_01360 at 294.136 kb on - strand, within IAI47_01360 at 294.136 kb on - strand, within IAI47_01360 at 294.202 kb on - strand at 294.513 kb on + strand, within IAI47_01365 at 294.514 kb on - strand, within IAI47_01365 at 294.578 kb on - strand, within IAI47_01365 at 294.664 kb on + strand, within IAI47_01365 at 294.904 kb on + strand, within IAI47_01365 at 295.108 kb on - strand, within IAI47_01365 at 295.201 kb on + strand, within IAI47_01365
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 292,907 + IAI47_01350 0.89 -2.2 292,907 + IAI47_01350 0.89 -0.4 292,907 + IAI47_01350 0.89 -0.9 292,908 - IAI47_01350 0.89 +0.7 292,908 - IAI47_01350 0.89 +0.3 292,908 - IAI47_01350 0.89 -0.9 292,908 - IAI47_01350 0.89 +0.5 292,908 - IAI47_01350 0.89 +1.9 292,972 + -1.2 292,972 + +2.0 292,972 + +0.2 292,972 + +2.6 292,972 + +0.5 292,973 - -0.9 292,973 - +0.3 292,973 - +1.8 292,973 - +0.7 292,973 - -1.7 292,973 - -0.6 292,976 + -0.0 292,976 + +0.2 292,976 + -0.0 292,976 + +1.5 292,976 + -1.5 292,976 + +0.3 292,976 + +2.7 292,977 - +0.5 292,977 - +0.8 292,977 - -0.5 292,995 - +0.1 292,996 + +1.2 293,023 + -1.5 293,023 + +0.1 293,024 - +1.3 293,091 + IAI47_01355 0.10 -1.4 293,100 + IAI47_01355 0.12 -1.8 293,100 + IAI47_01355 0.12 -2.8 293,100 + IAI47_01355 0.12 +0.6 293,101 - IAI47_01355 0.12 -3.1 293,180 + IAI47_01355 0.30 -1.5 293,180 + IAI47_01355 0.30 -3.0 293,180 + IAI47_01355 0.30 -1.1 293,180 + IAI47_01355 0.30 -1.8 293,180 + IAI47_01355 0.30 -3.1 293,181 - IAI47_01355 0.31 -1.2 293,181 - IAI47_01355 0.31 -0.1 293,181 - IAI47_01355 0.31 -2.4 293,301 + IAI47_01355 0.58 -2.8 293,301 + IAI47_01355 0.58 -1.0 293,302 - IAI47_01355 0.58 -1.0 293,313 + IAI47_01355 0.61 -2.0 293,313 + IAI47_01355 0.61 -1.9 293,313 + IAI47_01355 0.61 -1.5 293,313 + IAI47_01355 0.61 -1.5 293,313 + IAI47_01355 0.61 -0.3 293,314 - IAI47_01355 0.61 +0.8 293,320 + IAI47_01355 0.62 +0.8 293,345 + IAI47_01355 0.68 -0.4 293,358 - IAI47_01355 0.71 -1.5 293,358 - IAI47_01355 0.71 -1.7 293,397 - IAI47_01355 0.80 -3.3 293,397 - IAI47_01355 0.80 -1.4 293,455 + -1.4 293,455 + -1.8 293,455 + -2.6 293,456 - -1.1 293,456 - -0.6 293,475 + -2.9 293,475 + -0.2 293,475 + -2.1 293,476 - -0.9 293,476 - -0.7 293,476 - -1.5 293,476 - -0.1 293,476 - -3.3 293,476 - -2.7 293,477 + -2.2 293,477 + -2.7 293,477 + -1.2 293,478 - -1.7 293,478 - -2.0 293,478 - +1.5 293,478 - -2.3 293,478 - -1.7 293,478 - -1.7 293,487 + -1.6 293,487 + -0.1 293,487 + -2.2 293,488 - -0.1 293,508 - -2.2 293,544 + -1.0 293,545 - -1.5 293,618 + -0.7 293,618 + -2.5 293,619 - -1.1 293,620 + -0.3 293,620 + -2.5 293,620 + -1.3 293,620 + -1.7 293,621 - +0.1 293,621 - -0.4 293,629 + -1.8 293,629 + +0.3 293,630 - -1.5 293,630 - -2.7 293,630 - -1.5 293,631 + +0.8 293,631 + -0.5 293,632 - +0.3 293,632 - +0.1 293,632 - -2.1 293,632 - +2.1 293,632 - +1.3 293,647 + +0.9 293,647 + +0.9 293,673 - +1.7 293,678 + +1.4 293,678 + -1.3 293,678 + +1.9 293,679 - -0.5 293,679 - -0.0 293,679 - -2.0 293,679 - -1.0 293,679 - +0.8 293,691 - -0.6 293,702 - +0.4 293,702 - -1.7 293,702 - -1.9 293,751 + -0.0 293,751 + +3.0 293,752 - +1.8 293,898 + +1.5 293,898 + -2.9 293,898 + -1.1 293,898 + +0.5 293,898 + +0.9 293,898 + -0.2 293,899 - +0.3 293,899 - -0.1 293,899 - +2.2 293,899 - -0.6 293,899 - -2.3 293,899 - +0.2 293,899 - -1.4 293,899 - -0.1 293,899 - +0.0 293,910 + -2.9 293,910 + +1.2 293,911 - -0.7 293,911 - -1.1 293,943 + IAI47_01360 0.13 +1.6 293,943 + IAI47_01360 0.13 +0.1 293,943 + IAI47_01360 0.13 -2.0 293,943 + IAI47_01360 0.13 -1.1 293,944 - IAI47_01360 0.13 +1.7 293,944 - IAI47_01360 0.13 -1.6 293,944 - IAI47_01360 0.13 +0.5 293,944 - IAI47_01360 0.13 -2.1 293,944 - IAI47_01360 0.13 -1.1 293,952 + IAI47_01360 0.16 +0.3 293,952 + IAI47_01360 0.16 +1.2 293,952 + IAI47_01360 0.16 -0.8 293,984 + IAI47_01360 0.25 +1.1 293,984 + IAI47_01360 0.25 -1.1 293,993 + IAI47_01360 0.28 +1.3 293,997 - IAI47_01360 0.29 +1.3 294,019 + IAI47_01360 0.36 +0.7 294,019 + IAI47_01360 0.36 +1.0 294,019 + IAI47_01360 0.36 -0.4 294,020 - IAI47_01360 0.36 -0.2 294,020 - IAI47_01360 0.36 +1.9 294,020 - IAI47_01360 0.36 -3.1 294,020 - IAI47_01360 0.36 +3.4 294,025 + IAI47_01360 0.38 -0.8 294,025 + IAI47_01360 0.38 +1.2 294,025 + IAI47_01360 0.38 -1.5 294,025 + IAI47_01360 0.38 +0.3 294,025 + IAI47_01360 0.38 -0.9 294,025 + IAI47_01360 0.38 +1.0 294,025 + IAI47_01360 0.38 -0.4 294,025 + IAI47_01360 0.38 +0.0 294,026 - IAI47_01360 0.38 -0.7 294,026 - IAI47_01360 0.38 +2.9 294,026 - IAI47_01360 0.38 +0.7 294,026 - IAI47_01360 0.38 +1.1 294,026 - IAI47_01360 0.38 -0.6 294,026 - IAI47_01360 0.38 -0.8 294,026 - IAI47_01360 0.38 +1.4 294,026 - IAI47_01360 0.38 -2.7 294,026 - IAI47_01360 0.38 +0.2 294,091 + IAI47_01360 0.57 +0.1 294,091 + IAI47_01360 0.57 +2.5 294,091 + IAI47_01360 0.57 -0.0 294,091 + IAI47_01360 0.57 +1.4 294,091 + IAI47_01360 0.57 -1.9 294,091 + IAI47_01360 0.57 +2.9 294,091 + IAI47_01360 0.57 -1.3 294,091 + IAI47_01360 0.57 +1.5 294,091 + IAI47_01360 0.57 -0.5 294,091 + IAI47_01360 0.57 -3.0 294,091 + IAI47_01360 0.57 +0.1 294,091 + IAI47_01360 0.57 +1.9 294,091 + IAI47_01360 0.57 +1.7 294,092 - IAI47_01360 0.57 -2.3 294,092 - IAI47_01360 0.57 +0.9 294,092 - IAI47_01360 0.57 -0.7 294,092 - IAI47_01360 0.57 +0.3 294,092 - IAI47_01360 0.57 -1.3 294,092 - IAI47_01360 0.57 -2.3 294,092 - IAI47_01360 0.57 +1.5 294,092 - IAI47_01360 0.57 +3.6 294,092 - IAI47_01360 0.57 -1.8 294,092 - IAI47_01360 0.57 +0.1 294,092 - IAI47_01360 0.57 +2.2 294,092 - IAI47_01360 0.57 +1.7 294,092 - IAI47_01360 0.57 -1.6 294,092 - IAI47_01360 0.57 -1.1 294,092 - IAI47_01360 0.57 +1.1 294,092 - IAI47_01360 0.57 +0.2 294,092 - IAI47_01360 0.57 -1.7 294,092 - IAI47_01360 0.57 +0.6 294,092 - IAI47_01360 0.57 -0.5 294,092 - IAI47_01360 0.57 +0.8 294,092 - IAI47_01360 0.57 -1.6 294,092 - IAI47_01360 0.57 +1.2 294,092 - IAI47_01360 0.57 -1.0 294,093 + IAI47_01360 0.58 -1.3 294,093 + IAI47_01360 0.58 -1.4 294,093 + IAI47_01360 0.58 -1.2 294,093 + IAI47_01360 0.58 -0.1 294,093 + IAI47_01360 0.58 -1.2 294,093 + IAI47_01360 0.58 +2.5 294,093 + IAI47_01360 0.58 +0.1 294,094 - IAI47_01360 0.58 +0.7 294,094 - IAI47_01360 0.58 -0.1 294,094 - IAI47_01360 0.58 +1.5 294,094 - IAI47_01360 0.58 -0.5 294,094 - IAI47_01360 0.58 +1.0 294,094 - IAI47_01360 0.58 +1.7 294,094 - IAI47_01360 0.58 +1.2 294,135 + IAI47_01360 0.70 +0.3 294,136 - IAI47_01360 0.71 -1.8 294,136 - IAI47_01360 0.71 +0.8 294,202 - +0.5 294,513 + IAI47_01365 0.16 +2.5 294,514 - IAI47_01365 0.16 +4.5 294,578 - IAI47_01365 0.20 -0.5 294,664 + IAI47_01365 0.26 +2.7 294,904 + IAI47_01365 0.42 +2.4 295,108 - IAI47_01365 0.56 +3.1 295,201 + IAI47_01365 0.62 +4.7
Or see this region's nucleotide sequence