Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02825 and IAI47_02830 are separated by 52 nucleotides IAI47_02830 and IAI47_02835 are separated by 14 nucleotides IAI47_02835 and IAI47_02840 are separated by 14 nucleotides IAI47_02840 and IAI47_02845 overlap by 43 nucleotides
IAI47_02825: IAI47_02825 - pirin family protein, at 584,352 to 585,056
_02825
IAI47_02830: IAI47_02830 - divisome-associated lipoprotein YraP, at 585,109 to 585,681
_02830
IAI47_02835: IAI47_02835 - DnaA initiator-associating protein DiaA, at 585,696 to 586,283
_02835
IAI47_02840: IAI47_02840 - YraN family protein, at 586,298 to 586,693
_02840
IAI47_02845: IAI47_02845 - penicillin-binding protein activator, at 586,651 to 588,693
_02845
Position (kb) 585
586
587 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.701 kb on + strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.702 kb on - strand, within IAI47_02825 at 584.781 kb on - strand, within IAI47_02825 at 584.781 kb on - strand, within IAI47_02825 at 584.800 kb on + strand, within IAI47_02825 at 584.800 kb on + strand, within IAI47_02825 at 584.800 kb on + strand, within IAI47_02825 at 584.801 kb on - strand, within IAI47_02825 at 584.898 kb on + strand, within IAI47_02825 at 584.898 kb on + strand, within IAI47_02825 at 584.899 kb on - strand, within IAI47_02825 at 584.899 kb on - strand, within IAI47_02825 at 584.899 kb on - strand, within IAI47_02825 at 584.899 kb on - strand, within IAI47_02825 at 585.044 kb on + strand at 585.045 kb on - strand at 585.045 kb on - strand at 585.045 kb on - strand at 585.068 kb on + strand at 585.069 kb on - strand at 585.103 kb on - strand at 585.103 kb on - strand at 585.103 kb on - strand at 585.110 kb on + strand at 585.110 kb on + strand at 585.110 kb on + strand at 585.111 kb on - strand at 585.111 kb on - strand at 585.111 kb on - strand at 585.123 kb on + strand at 585.123 kb on + strand at 585.123 kb on + strand at 585.123 kb on + strand at 585.123 kb on + strand at 585.123 kb on + strand at 585.123 kb on + strand at 585.124 kb on - strand at 585.361 kb on - strand, within IAI47_02830 at 585.362 kb on + strand, within IAI47_02830 at 585.362 kb on + strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.363 kb on - strand, within IAI47_02830 at 585.529 kb on + strand, within IAI47_02830 at 585.529 kb on + strand, within IAI47_02830 at 585.529 kb on + strand, within IAI47_02830 at 585.558 kb on + strand, within IAI47_02830 at 585.558 kb on + strand, within IAI47_02830 at 585.559 kb on - strand, within IAI47_02830 at 585.637 kb on + strand at 585.637 kb on + strand at 585.637 kb on + strand at 585.637 kb on + strand at 585.638 kb on - strand at 585.638 kb on - strand at 585.638 kb on - strand at 585.638 kb on - strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.657 kb on + strand at 585.658 kb on - strand at 585.658 kb on - strand at 585.658 kb on - strand at 585.658 kb on - strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.661 kb on + strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.662 kb on - strand at 585.663 kb on + strand at 585.663 kb on + strand at 585.663 kb on + strand at 585.664 kb on - strand at 585.664 kb on - strand at 585.722 kb on + strand at 585.738 kb on + strand at 585.738 kb on + strand at 585.739 kb on - strand at 585.739 kb on - strand at 585.746 kb on + strand at 585.746 kb on + strand at 585.747 kb on - strand at 585.747 kb on - strand at 585.747 kb on - strand at 585.758 kb on + strand, within IAI47_02835 at 585.758 kb on + strand, within IAI47_02835 at 585.758 kb on + strand, within IAI47_02835 at 585.758 kb on + strand, within IAI47_02835 at 585.758 kb on + strand, within IAI47_02835 at 585.758 kb on + strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.759 kb on - strand, within IAI47_02835 at 585.773 kb on + strand, within IAI47_02835 at 585.851 kb on - strand, within IAI47_02835 at 585.869 kb on + strand, within IAI47_02835 at 585.869 kb on + strand, within IAI47_02835 at 585.870 kb on - strand, within IAI47_02835 at 585.870 kb on - strand, within IAI47_02835 at 585.875 kb on + strand, within IAI47_02835 at 585.898 kb on - strand, within IAI47_02835 at 585.898 kb on - strand, within IAI47_02835 at 585.906 kb on - strand, within IAI47_02835 at 585.946 kb on - strand, within IAI47_02835 at 585.975 kb on + strand, within IAI47_02835 at 585.975 kb on + strand, within IAI47_02835 at 585.976 kb on - strand, within IAI47_02835 at 585.976 kb on - strand, within IAI47_02835 at 585.976 kb on - strand, within IAI47_02835 at 585.976 kb on - strand, within IAI47_02835 at 585.988 kb on + strand, within IAI47_02835 at 585.988 kb on + strand, within IAI47_02835 at 585.988 kb on + strand, within IAI47_02835 at 585.988 kb on + strand, within IAI47_02835 at 585.989 kb on - strand, within IAI47_02835 at 585.989 kb on - strand, within IAI47_02835 at 586.047 kb on + strand, within IAI47_02835 at 586.047 kb on + strand, within IAI47_02835 at 586.048 kb on - strand, within IAI47_02835 at 586.158 kb on - strand, within IAI47_02835 at 586.158 kb on - strand, within IAI47_02835 at 586.248 kb on - strand at 586.256 kb on + strand at 586.257 kb on - strand at 586.268 kb on + strand at 586.268 kb on + strand at 586.268 kb on + strand at 586.268 kb on + strand at 586.269 kb on - strand at 586.269 kb on - strand at 586.269 kb on - strand at 586.269 kb on - strand at 586.299 kb on + strand at 586.299 kb on + strand at 586.300 kb on - strand at 586.300 kb on - strand at 586.300 kb on - strand at 586.312 kb on + strand at 586.313 kb on - strand at 586.313 kb on - strand at 586.445 kb on - strand, within IAI47_02840 at 586.445 kb on - strand, within IAI47_02840 at 586.445 kb on - strand, within IAI47_02840 at 586.476 kb on + strand, within IAI47_02840 at 586.534 kb on + strand, within IAI47_02840 at 586.535 kb on - strand, within IAI47_02840 at 586.535 kb on - strand, within IAI47_02840 at 586.535 kb on - strand, within IAI47_02840 at 586.535 kb on - strand, within IAI47_02840 at 586.570 kb on + strand, within IAI47_02840 at 586.570 kb on + strand, within IAI47_02840 at 586.570 kb on + strand, within IAI47_02840 at 586.571 kb on - strand, within IAI47_02840 at 586.571 kb on - strand, within IAI47_02840 at 586.583 kb on + strand, within IAI47_02840 at 586.584 kb on - strand, within IAI47_02840 at 586.584 kb on - strand, within IAI47_02840 at 586.606 kb on - strand, within IAI47_02840 at 586.620 kb on - strand, within IAI47_02840 at 586.652 kb on + strand at 586.652 kb on + strand at 586.652 kb on + strand at 586.653 kb on - strand at 586.653 kb on - strand at 586.653 kb on - strand at 586.653 kb on - strand at 586.738 kb on - strand at 586.786 kb on - strand at 586.826 kb on + strand at 586.827 kb on - strand at 586.827 kb on - strand at 586.827 kb on - strand at 586.844 kb on + strand at 586.852 kb on - strand at 586.988 kb on + strand, within IAI47_02845 at 587.072 kb on + strand, within IAI47_02845 at 587.072 kb on + strand, within IAI47_02845 at 587.072 kb on + strand, within IAI47_02845 at 587.073 kb on - strand, within IAI47_02845 at 587.073 kb on - strand, within IAI47_02845 at 587.073 kb on - strand, within IAI47_02845 at 587.073 kb on - strand, within IAI47_02845 at 587.073 kb on - strand, within IAI47_02845 at 587.157 kb on + strand, within IAI47_02845 at 587.157 kb on + strand, within IAI47_02845 at 587.158 kb on - strand, within IAI47_02845 at 587.158 kb on - strand, within IAI47_02845 at 587.229 kb on + strand, within IAI47_02845 at 587.230 kb on - strand, within IAI47_02845
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 584,701 + IAI47_02825 0.50 -0.7 584,701 + IAI47_02825 0.50 -0.8 584,701 + IAI47_02825 0.50 +0.4 584,701 + IAI47_02825 0.50 +1.3 584,701 + IAI47_02825 0.50 +1.2 584,701 + IAI47_02825 0.50 -0.4 584,701 + IAI47_02825 0.50 +0.4 584,701 + IAI47_02825 0.50 +0.2 584,702 - IAI47_02825 0.50 +0.7 584,702 - IAI47_02825 0.50 +1.0 584,702 - IAI47_02825 0.50 -0.3 584,702 - IAI47_02825 0.50 +1.2 584,702 - IAI47_02825 0.50 -0.2 584,702 - IAI47_02825 0.50 +0.5 584,702 - IAI47_02825 0.50 +1.2 584,702 - IAI47_02825 0.50 +0.4 584,781 - IAI47_02825 0.61 +1.2 584,781 - IAI47_02825 0.61 -0.2 584,800 + IAI47_02825 0.64 -0.2 584,800 + IAI47_02825 0.64 +1.6 584,800 + IAI47_02825 0.64 +1.4 584,801 - IAI47_02825 0.64 -1.5 584,898 + IAI47_02825 0.77 -0.3 584,898 + IAI47_02825 0.77 -2.0 584,899 - IAI47_02825 0.78 -0.2 584,899 - IAI47_02825 0.78 -0.8 584,899 - IAI47_02825 0.78 +1.1 584,899 - IAI47_02825 0.78 +0.1 585,044 + -0.3 585,045 - +0.1 585,045 - +0.6 585,045 - -0.3 585,068 + -1.7 585,069 - -0.6 585,103 - +1.0 585,103 - +0.1 585,103 - +0.4 585,110 + +2.9 585,110 + +1.0 585,110 + +0.1 585,111 - -0.9 585,111 - -1.0 585,111 - -0.2 585,123 + +2.3 585,123 + +0.3 585,123 + +0.6 585,123 + -0.9 585,123 + +1.1 585,123 + -0.0 585,123 + +0.0 585,124 - +0.4 585,361 - IAI47_02830 0.44 -1.1 585,362 + IAI47_02830 0.44 -1.9 585,362 + IAI47_02830 0.44 +0.3 585,363 - IAI47_02830 0.44 +0.2 585,363 - IAI47_02830 0.44 -0.9 585,363 - IAI47_02830 0.44 +1.7 585,363 - IAI47_02830 0.44 -0.7 585,363 - IAI47_02830 0.44 -0.0 585,363 - IAI47_02830 0.44 -2.1 585,363 - IAI47_02830 0.44 +0.5 585,363 - IAI47_02830 0.44 +0.5 585,363 - IAI47_02830 0.44 +0.8 585,363 - IAI47_02830 0.44 -1.9 585,529 + IAI47_02830 0.73 -1.4 585,529 + IAI47_02830 0.73 -0.3 585,529 + IAI47_02830 0.73 -0.1 585,558 + IAI47_02830 0.78 +0.9 585,558 + IAI47_02830 0.78 -0.5 585,559 - IAI47_02830 0.79 -0.0 585,637 + +1.4 585,637 + +0.3 585,637 + -1.3 585,637 + +0.2 585,638 - -1.5 585,638 - -0.8 585,638 - +0.0 585,638 - +2.1 585,657 + +0.5 585,657 + -1.0 585,657 + -0.2 585,657 + -1.1 585,657 + -0.4 585,657 + -0.7 585,657 + +0.8 585,657 + +0.1 585,657 + -1.3 585,658 - -0.6 585,658 - -0.6 585,658 - -1.1 585,658 - -0.4 585,661 + +1.1 585,661 + -0.1 585,661 + -0.2 585,661 + +1.2 585,661 + +1.0 585,661 + -0.5 585,661 + +0.1 585,661 + +0.4 585,661 + -0.5 585,662 - +0.3 585,662 - +0.5 585,662 - -0.5 585,662 - -1.1 585,662 - +0.5 585,662 - -0.1 585,662 - +0.7 585,662 - -2.1 585,662 - +0.3 585,662 - +0.1 585,662 - +0.8 585,662 - -0.2 585,662 - +0.5 585,662 - -1.3 585,663 + +0.6 585,663 + -0.3 585,663 + -0.9 585,664 - -1.3 585,664 - -0.3 585,722 + +1.5 585,738 + +1.6 585,738 + +2.2 585,739 - +0.9 585,739 - +1.3 585,746 + +0.7 585,746 + +1.7 585,747 - -1.6 585,747 - -3.5 585,747 - +1.1 585,758 + IAI47_02835 0.11 +1.6 585,758 + IAI47_02835 0.11 +3.1 585,758 + IAI47_02835 0.11 +0.3 585,758 + IAI47_02835 0.11 +2.0 585,758 + IAI47_02835 0.11 +1.2 585,758 + IAI47_02835 0.11 +2.1 585,759 - IAI47_02835 0.11 +1.0 585,759 - IAI47_02835 0.11 +0.1 585,759 - IAI47_02835 0.11 -0.7 585,759 - IAI47_02835 0.11 +0.5 585,759 - IAI47_02835 0.11 +2.0 585,759 - IAI47_02835 0.11 -0.4 585,759 - IAI47_02835 0.11 +0.5 585,759 - IAI47_02835 0.11 +1.1 585,759 - IAI47_02835 0.11 +0.3 585,759 - IAI47_02835 0.11 +1.8 585,759 - IAI47_02835 0.11 +0.9 585,759 - IAI47_02835 0.11 +1.8 585,759 - IAI47_02835 0.11 +1.1 585,759 - IAI47_02835 0.11 +1.0 585,759 - IAI47_02835 0.11 +2.8 585,759 - IAI47_02835 0.11 +0.7 585,759 - IAI47_02835 0.11 +0.2 585,773 + IAI47_02835 0.13 +1.7 585,851 - IAI47_02835 0.26 +1.5 585,869 + IAI47_02835 0.29 +1.2 585,869 + IAI47_02835 0.29 -0.6 585,870 - IAI47_02835 0.30 +1.3 585,870 - IAI47_02835 0.30 +2.3 585,875 + IAI47_02835 0.30 +1.7 585,898 - IAI47_02835 0.34 +0.2 585,898 - IAI47_02835 0.34 +0.7 585,906 - IAI47_02835 0.36 +2.2 585,946 - IAI47_02835 0.43 -0.5 585,975 + IAI47_02835 0.47 +0.5 585,975 + IAI47_02835 0.47 +0.4 585,976 - IAI47_02835 0.48 +2.7 585,976 - IAI47_02835 0.48 +0.7 585,976 - IAI47_02835 0.48 +1.7 585,976 - IAI47_02835 0.48 +1.2 585,988 + IAI47_02835 0.50 -0.2 585,988 + IAI47_02835 0.50 +0.1 585,988 + IAI47_02835 0.50 +0.4 585,988 + IAI47_02835 0.50 +0.3 585,989 - IAI47_02835 0.50 +1.9 585,989 - IAI47_02835 0.50 -0.3 586,047 + IAI47_02835 0.60 +0.3 586,047 + IAI47_02835 0.60 +0.4 586,048 - IAI47_02835 0.60 +0.5 586,158 - IAI47_02835 0.79 +1.1 586,158 - IAI47_02835 0.79 +0.5 586,248 - +0.7 586,256 + -1.4 586,257 - +2.2 586,268 + +1.2 586,268 + +2.1 586,268 + +1.0 586,268 + +0.6 586,269 - -0.7 586,269 - -1.3 586,269 - -0.1 586,269 - +0.9 586,299 + +2.0 586,299 + +0.1 586,300 - -0.6 586,300 - +0.5 586,300 - +0.2 586,312 + +0.4 586,313 - -0.5 586,313 - -0.6 586,445 - IAI47_02840 0.37 +0.4 586,445 - IAI47_02840 0.37 +2.5 586,445 - IAI47_02840 0.37 +0.7 586,476 + IAI47_02840 0.45 +0.8 586,534 + IAI47_02840 0.60 -0.7 586,535 - IAI47_02840 0.60 -1.1 586,535 - IAI47_02840 0.60 +0.5 586,535 - IAI47_02840 0.60 -1.8 586,535 - IAI47_02840 0.60 -1.1 586,570 + IAI47_02840 0.69 -1.2 586,570 + IAI47_02840 0.69 +0.2 586,570 + IAI47_02840 0.69 -0.3 586,571 - IAI47_02840 0.69 +1.2 586,571 - IAI47_02840 0.69 +0.4 586,583 + IAI47_02840 0.72 +1.6 586,584 - IAI47_02840 0.72 +0.9 586,584 - IAI47_02840 0.72 -0.6 586,606 - IAI47_02840 0.78 -1.6 586,620 - IAI47_02840 0.81 -2.4 586,652 + -0.5 586,652 + -0.5 586,652 + +2.7 586,653 - -0.6 586,653 - +0.6 586,653 - -0.8 586,653 - +0.4 586,738 - -0.7 586,786 - -0.4 586,826 + +2.0 586,827 - -0.1 586,827 - -0.0 586,827 - -1.0 586,844 + +1.4 586,852 - -2.0 586,988 + IAI47_02845 0.16 -0.1 587,072 + IAI47_02845 0.21 +1.0 587,072 + IAI47_02845 0.21 -1.0 587,072 + IAI47_02845 0.21 -0.5 587,073 - IAI47_02845 0.21 -1.8 587,073 - IAI47_02845 0.21 +0.8 587,073 - IAI47_02845 0.21 -1.6 587,073 - IAI47_02845 0.21 -0.5 587,073 - IAI47_02845 0.21 -0.6 587,157 + IAI47_02845 0.25 +0.0 587,157 + IAI47_02845 0.25 -0.4 587,158 - IAI47_02845 0.25 -0.2 587,158 - IAI47_02845 0.25 -0.8 587,229 + IAI47_02845 0.28 +0.2 587,230 - IAI47_02845 0.28 -1.5
Or see this region's nucleotide sequence