Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS08070

Experiment: McCas_formate_complete sodium bromide 125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS08060 and MMJJ_RS08065 are separated by 148 nucleotidesMMJJ_RS08065 and MMJJ_RS08070 are separated by 97 nucleotidesMMJJ_RS08070 and MMJJ_RS08075 are separated by 122 nucleotides MMJJ_RS08060: MMJJ_RS08060 - DNA repair and recombination protein RadA, at 1,476,202 to 1,477,170 _RS08060 MMJJ_RS08065: MMJJ_RS08065 - precorrin-6Y C5,15-methyltransferase (decarboxylating) subunit CbiT, at 1,477,319 to 1,477,864 _RS08065 MMJJ_RS08070: MMJJ_RS08070 - alpha-glucan family phosphorylase, at 1,477,962 to 1,479,524 _RS08070 MMJJ_RS08075: MMJJ_RS08075 - ATP-dependent DNA helicase, at 1,479,647 to 1,481,656 _RS08075 Position (kb) 1477 1478 1479 1480Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 1477.020 kb on - strand, within MMJJ_RS08060at 1477.041 kb on - strand, within MMJJ_RS08060at 1477.051 kb on + strand, within MMJJ_RS08060at 1477.170 kb on - strandat 1477.191 kb on - strandat 1477.192 kb on + strandat 1477.203 kb on + strandat 1477.203 kb on + strandat 1477.206 kb on + strandat 1477.206 kb on + strandat 1477.206 kb on + strandat 1477.206 kb on + strandat 1477.208 kb on - strandat 1477.208 kb on - strandat 1477.208 kb on - strandat 1477.208 kb on - strandat 1477.208 kb on - strandat 1477.209 kb on + strandat 1477.209 kb on + strandat 1477.209 kb on + strandat 1477.214 kb on - strandat 1477.215 kb on + strandat 1477.215 kb on + strandat 1477.237 kb on - strandat 1477.248 kb on - strandat 1477.249 kb on + strandat 1477.253 kb on - strandat 1477.253 kb on - strandat 1477.253 kb on - strandat 1477.254 kb on + strandat 1477.254 kb on + strandat 1477.254 kb on + strandat 1477.254 kb on + strandat 1477.258 kb on - strandat 1477.258 kb on - strandat 1477.258 kb on - strandat 1477.259 kb on + strandat 1477.259 kb on + strandat 1477.259 kb on + strandat 1477.259 kb on + strandat 1477.259 kb on + strandat 1477.260 kb on - strandat 1477.260 kb on - strandat 1477.260 kb on - strandat 1477.260 kb on - strandat 1477.260 kb on - strandat 1477.260 kb on - strandat 1477.261 kb on + strandat 1477.261 kb on + strandat 1477.261 kb on + strandat 1477.261 kb on + strandat 1477.265 kb on + strandat 1477.278 kb on + strandat 1477.281 kb on - strandat 1477.283 kb on - strandat 1477.689 kb on - strand, within MMJJ_RS08065at 1477.770 kb on - strand, within MMJJ_RS08065at 1477.815 kb on - strandat 1477.863 kb on + strandat 1477.872 kb on - strandat 1477.886 kb on - strandat 1477.888 kb on - strandat 1477.889 kb on + strandat 1477.893 kb on + strandat 1477.896 kb on + strandat 1477.899 kb on - strandat 1477.900 kb on + strandat 1477.900 kb on + strandat 1477.900 kb on + strandat 1477.900 kb on + strandat 1477.901 kb on - strandat 1477.902 kb on + strandat 1477.906 kb on - strandat 1477.906 kb on - strandat 1477.907 kb on + strandat 1477.907 kb on + strandat 1477.915 kb on + strandat 1477.917 kb on - strandat 1477.917 kb on - strandat 1477.918 kb on + strandat 1477.920 kb on - strandat 1477.935 kb on + strandat 1477.941 kb on - strandat 1477.942 kb on + strandat 1477.947 kb on + strandat 1477.977 kb on - strandat 1477.983 kb on + strandat 1478.019 kb on - strandat 1478.020 kb on + strandat 1478.042 kb on + strandat 1478.081 kb on + strandat 1478.081 kb on + strandat 1478.121 kb on - strand, within MMJJ_RS08070at 1478.151 kb on + strand, within MMJJ_RS08070at 1478.165 kb on - strand, within MMJJ_RS08070at 1478.168 kb on + strand, within MMJJ_RS08070at 1478.175 kb on - strand, within MMJJ_RS08070at 1478.289 kb on - strand, within MMJJ_RS08070at 1478.306 kb on - strand, within MMJJ_RS08070at 1478.352 kb on - strand, within MMJJ_RS08070at 1478.363 kb on - strand, within MMJJ_RS08070at 1478.368 kb on - strand, within MMJJ_RS08070at 1478.445 kb on - strand, within MMJJ_RS08070at 1478.452 kb on - strand, within MMJJ_RS08070at 1478.470 kb on - strand, within MMJJ_RS08070at 1478.512 kb on + strand, within MMJJ_RS08070at 1478.516 kb on + strand, within MMJJ_RS08070at 1478.516 kb on + strand, within MMJJ_RS08070at 1478.527 kb on - strand, within MMJJ_RS08070at 1478.528 kb on + strand, within MMJJ_RS08070at 1478.528 kb on + strand, within MMJJ_RS08070at 1478.535 kb on + strand, within MMJJ_RS08070at 1478.576 kb on - strand, within MMJJ_RS08070at 1478.594 kb on - strand, within MMJJ_RS08070at 1478.762 kb on - strand, within MMJJ_RS08070at 1478.793 kb on - strand, within MMJJ_RS08070at 1478.793 kb on - strand, within MMJJ_RS08070at 1478.793 kb on - strand, within MMJJ_RS08070at 1478.794 kb on + strand, within MMJJ_RS08070at 1478.968 kb on + strand, within MMJJ_RS08070at 1478.968 kb on + strand, within MMJJ_RS08070at 1479.009 kb on + strand, within MMJJ_RS08070at 1479.086 kb on - strand, within MMJJ_RS08070at 1479.148 kb on + strand, within MMJJ_RS08070at 1479.148 kb on + strand, within MMJJ_RS08070at 1479.155 kb on + strand, within MMJJ_RS08070at 1479.172 kb on - strand, within MMJJ_RS08070at 1479.173 kb on + strand, within MMJJ_RS08070at 1479.191 kb on + strand, within MMJJ_RS08070at 1479.191 kb on + strand, within MMJJ_RS08070at 1479.191 kb on + strand, within MMJJ_RS08070at 1479.191 kb on + strand, within MMJJ_RS08070at 1479.276 kb on + strand, within MMJJ_RS08070at 1479.329 kb on - strand, within MMJJ_RS08070at 1479.381 kb on - strandat 1479.384 kb on + strandat 1479.402 kb on + strandat 1479.402 kb on + strandat 1479.403 kb on - strandat 1479.404 kb on + strandat 1479.412 kb on + strandat 1479.469 kb on + strandat 1479.501 kb on + strandat 1479.523 kb on + strandat 1479.584 kb on - strandat 1479.585 kb on + strandat 1479.585 kb on + strandat 1479.585 kb on + strandat 1479.588 kb on - strandat 1479.588 kb on - strandat 1479.588 kb on - strandat 1479.588 kb on - strandat 1479.588 kb on - strandat 1479.588 kb on - strandat 1479.588 kb on - strandat 1479.589 kb on + strandat 1479.590 kb on - strandat 1479.590 kb on - strandat 1479.591 kb on + strandat 1479.591 kb on + strandat 1479.591 kb on + strandat 1479.591 kb on + strandat 1479.593 kb on + strandat 1479.607 kb on - strandat 1479.625 kb on + strandat 1479.635 kb on - strandat 1479.825 kb on + strandat 1480.003 kb on + strand, within MMJJ_RS08075at 1480.088 kb on + strand, within MMJJ_RS08075at 1480.156 kb on - strand, within MMJJ_RS08075at 1480.157 kb on + strand, within MMJJ_RS08075at 1480.157 kb on + strand, within MMJJ_RS08075at 1480.178 kb on + strand, within MMJJ_RS08075at 1480.178 kb on + strand, within MMJJ_RS08075at 1480.191 kb on - strand, within MMJJ_RS08075at 1480.192 kb on + strand, within MMJJ_RS08075at 1480.192 kb on + strand, within MMJJ_RS08075at 1480.231 kb on + strand, within MMJJ_RS08075at 1480.290 kb on - strand, within MMJJ_RS08075

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete sodium bromide 125 mM
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1,477,020 - MMJJ_RS08060 0.84 -1.3
1,477,041 - MMJJ_RS08060 0.87 -1.0
1,477,051 + MMJJ_RS08060 0.88 -2.7
1,477,170 - +0.3
1,477,191 - -2.8
1,477,192 + -2.8
1,477,203 + +1.3
1,477,203 + -0.8
1,477,206 + -2.2
1,477,206 + -3.2
1,477,206 + +0.6
1,477,206 + -0.7
1,477,208 - -1.7
1,477,208 - -3.6
1,477,208 - -1.3
1,477,208 - -0.3
1,477,208 - -2.2
1,477,209 + -2.0
1,477,209 + -2.7
1,477,209 + -3.2
1,477,214 - -2.2
1,477,215 + -3.0
1,477,215 + -2.3
1,477,237 - +0.1
1,477,248 - -3.4
1,477,249 + -2.3
1,477,253 - -0.7
1,477,253 - -2.6
1,477,253 - -1.6
1,477,254 + -0.1
1,477,254 + -0.9
1,477,254 + +1.3
1,477,254 + +1.2
1,477,258 - +2.4
1,477,258 - +2.1
1,477,258 - -1.2
1,477,259 + +0.9
1,477,259 + +3.0
1,477,259 + -0.6
1,477,259 + -1.5
1,477,259 + -0.5
1,477,260 - -0.1
1,477,260 - +1.4
1,477,260 - -2.7
1,477,260 - -3.4
1,477,260 - -3.0
1,477,260 - -1.3
1,477,261 + -0.6
1,477,261 + -0.1
1,477,261 + -0.5
1,477,261 + +0.3
1,477,265 + -0.8
1,477,278 + -1.3
1,477,281 - -3.3
1,477,283 - +1.5
1,477,689 - MMJJ_RS08065 0.68 +4.5
1,477,770 - MMJJ_RS08065 0.83 -1.7
1,477,815 - +1.1
1,477,863 + +2.4
1,477,872 - +3.7
1,477,886 - -0.6
1,477,888 - -0.8
1,477,889 + -2.9
1,477,893 + +2.0
1,477,896 + +0.8
1,477,899 - -1.7
1,477,900 + +2.6
1,477,900 + -2.3
1,477,900 + -2.9
1,477,900 + -1.4
1,477,901 - -1.7
1,477,902 + -2.8
1,477,906 - +3.4
1,477,906 - -1.3
1,477,907 + +2.1
1,477,907 + -3.5
1,477,915 + +0.2
1,477,917 - -3.4
1,477,917 - -2.6
1,477,918 + -2.9
1,477,920 - +2.4
1,477,935 + +3.3
1,477,941 - +0.9
1,477,942 + +2.1
1,477,947 + -3.4
1,477,977 - +2.3
1,477,983 + -1.9
1,478,019 - +3.1
1,478,020 + -0.7
1,478,042 + +1.3
1,478,081 + +0.3
1,478,081 + +3.4
1,478,121 - MMJJ_RS08070 0.10 -2.3
1,478,151 + MMJJ_RS08070 0.12 +1.7
1,478,165 - MMJJ_RS08070 0.13 +2.9
1,478,168 + MMJJ_RS08070 0.13 -0.3
1,478,175 - MMJJ_RS08070 0.14 +3.6
1,478,289 - MMJJ_RS08070 0.21 -3.8
1,478,306 - MMJJ_RS08070 0.22 -2.5
1,478,352 - MMJJ_RS08070 0.25 +2.1
1,478,363 - MMJJ_RS08070 0.26 -0.3
1,478,368 - MMJJ_RS08070 0.26 -1.8
1,478,445 - MMJJ_RS08070 0.31 +0.7
1,478,452 - MMJJ_RS08070 0.31 +0.4
1,478,470 - MMJJ_RS08070 0.33 +4.2
1,478,512 + MMJJ_RS08070 0.35 -2.4
1,478,516 + MMJJ_RS08070 0.35 -2.7
1,478,516 + MMJJ_RS08070 0.35 +2.2
1,478,527 - MMJJ_RS08070 0.36 -2.4
1,478,528 + MMJJ_RS08070 0.36 +2.0
1,478,528 + MMJJ_RS08070 0.36 -1.0
1,478,535 + MMJJ_RS08070 0.37 -4.0
1,478,576 - MMJJ_RS08070 0.39 +1.3
1,478,594 - MMJJ_RS08070 0.40 +1.1
1,478,762 - MMJJ_RS08070 0.51 -0.7
1,478,793 - MMJJ_RS08070 0.53 -0.5
1,478,793 - MMJJ_RS08070 0.53 -1.0
1,478,793 - MMJJ_RS08070 0.53 +0.0
1,478,794 + MMJJ_RS08070 0.53 -2.2
1,478,968 + MMJJ_RS08070 0.64 +2.5
1,478,968 + MMJJ_RS08070 0.64 +4.9
1,479,009 + MMJJ_RS08070 0.67 -1.7
1,479,086 - MMJJ_RS08070 0.72 -1.5
1,479,148 + MMJJ_RS08070 0.76 +3.2
1,479,148 + MMJJ_RS08070 0.76 +0.4
1,479,155 + MMJJ_RS08070 0.76 +2.6
1,479,172 - MMJJ_RS08070 0.77 +2.2
1,479,173 + MMJJ_RS08070 0.77 +1.3
1,479,191 + MMJJ_RS08070 0.79 +2.7
1,479,191 + MMJJ_RS08070 0.79 -4.2
1,479,191 + MMJJ_RS08070 0.79 -2.0
1,479,191 + MMJJ_RS08070 0.79 -3.0
1,479,276 + MMJJ_RS08070 0.84 +0.2
1,479,329 - MMJJ_RS08070 0.87 +0.1
1,479,381 - +5.1
1,479,384 + -0.7
1,479,402 + +0.6
1,479,402 + -0.3
1,479,403 - +2.5
1,479,404 + +0.1
1,479,412 + +4.7
1,479,469 + -2.0
1,479,501 + +2.6
1,479,523 + -3.0
1,479,584 - -0.9
1,479,585 + +1.9
1,479,585 + -2.7
1,479,585 + +1.3
1,479,588 - -2.0
1,479,588 - -3.7
1,479,588 - +0.4
1,479,588 - +1.3
1,479,588 - -1.8
1,479,588 - -1.5
1,479,588 - +0.4
1,479,589 + +0.3
1,479,590 - -0.6
1,479,590 - +0.4
1,479,591 + +1.8
1,479,591 + -1.9
1,479,591 + +0.5
1,479,591 + -1.7
1,479,593 + +3.1
1,479,607 - -2.3
1,479,625 + +4.8
1,479,635 - +1.0
1,479,825 + +2.3
1,480,003 + MMJJ_RS08075 0.18 -3.0
1,480,088 + MMJJ_RS08075 0.22 +1.0
1,480,156 - MMJJ_RS08075 0.25 +0.1
1,480,157 + MMJJ_RS08075 0.25 -1.0
1,480,157 + MMJJ_RS08075 0.25 -2.7
1,480,178 + MMJJ_RS08075 0.26 -1.7
1,480,178 + MMJJ_RS08075 0.26 -3.3
1,480,191 - MMJJ_RS08075 0.27 +2.1
1,480,192 + MMJJ_RS08075 0.27 -3.8
1,480,192 + MMJJ_RS08075 0.27 -1.5
1,480,231 + MMJJ_RS08075 0.29 -2.6
1,480,290 - MMJJ_RS08075 0.32 +1.9

Or see this region's nucleotide sequence