Experiment: McCas_formate_complete sodium bromide 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07960 and MMJJ_RS07965 are separated by 10 nucleotides MMJJ_RS07965 and MMJJ_RS07970 are separated by 5 nucleotides MMJJ_RS07970 and MMJJ_RS07975 are separated by 27 nucleotides MMJJ_RS07975 and MMJJ_RS07980 overlap by 4 nucleotides
MMJJ_RS07960: MMJJ_RS07960 - quinolinate synthase NadA, at 1,459,928 to 1,460,845
_RS07960
MMJJ_RS07965: MMJJ_RS07965 - hypothetical protein, at 1,460,856 to 1,461,215
_RS07965
MMJJ_RS07970: MMJJ_RS07970 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,461,221 to 1,462,363
_RS07970
MMJJ_RS07975: MMJJ_RS07975 - ATP-dependent sacrificial sulfur transferase LarE, at 1,462,391 to 1,463,167
_RS07975
MMJJ_RS07980: MMJJ_RS07980 - biotin synthase BioB, at 1,463,164 to 1,464,147
_RS07980
Position (kb)
1461
1462
1463 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1460.264 kb on + strand, within MMJJ_RS07960 at 1460.512 kb on + strand, within MMJJ_RS07960 at 1460.740 kb on + strand, within MMJJ_RS07960 at 1460.872 kb on - strand at 1460.873 kb on + strand at 1460.873 kb on + strand at 1460.875 kb on - strand at 1460.917 kb on - strand, within MMJJ_RS07965 at 1460.918 kb on + strand, within MMJJ_RS07965 at 1460.920 kb on - strand, within MMJJ_RS07965 at 1460.950 kb on + strand, within MMJJ_RS07965 at 1460.953 kb on - strand, within MMJJ_RS07965 at 1460.955 kb on - strand, within MMJJ_RS07965 at 1460.956 kb on + strand, within MMJJ_RS07965 at 1460.956 kb on + strand, within MMJJ_RS07965 at 1461.040 kb on - strand, within MMJJ_RS07965 at 1461.051 kb on - strand, within MMJJ_RS07965 at 1461.051 kb on - strand, within MMJJ_RS07965 at 1461.074 kb on + strand, within MMJJ_RS07965 at 1461.207 kb on + strand at 1462.053 kb on + strand, within MMJJ_RS07970 at 1462.093 kb on + strand, within MMJJ_RS07970 at 1462.246 kb on + strand, within MMJJ_RS07970 at 1462.299 kb on + strand at 1462.327 kb on - strand at 1462.345 kb on - strand at 1462.364 kb on + strand at 1462.388 kb on - strand at 1462.392 kb on - strand at 1462.392 kb on - strand at 1462.393 kb on + strand at 1462.412 kb on - strand at 1462.412 kb on - strand at 1462.413 kb on + strand at 1462.431 kb on + strand at 1462.447 kb on - strand at 1462.451 kb on - strand at 1462.451 kb on - strand at 1462.454 kb on - strand at 1462.454 kb on - strand at 1462.455 kb on + strand at 1462.499 kb on + strand, within MMJJ_RS07975 at 1462.510 kb on - strand, within MMJJ_RS07975 at 1462.545 kb on - strand, within MMJJ_RS07975 at 1462.572 kb on + strand, within MMJJ_RS07975 at 1462.603 kb on - strand, within MMJJ_RS07975 at 1462.604 kb on + strand, within MMJJ_RS07975 at 1462.651 kb on - strand, within MMJJ_RS07975 at 1462.652 kb on + strand, within MMJJ_RS07975 at 1462.680 kb on - strand, within MMJJ_RS07975 at 1462.681 kb on + strand, within MMJJ_RS07975 at 1462.754 kb on + strand, within MMJJ_RS07975 at 1462.764 kb on + strand, within MMJJ_RS07975 at 1462.784 kb on - strand, within MMJJ_RS07975 at 1462.786 kb on - strand, within MMJJ_RS07975 at 1462.821 kb on - strand, within MMJJ_RS07975 at 1462.821 kb on - strand, within MMJJ_RS07975 at 1462.846 kb on - strand, within MMJJ_RS07975 at 1463.059 kb on - strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.234 kb on - strand at 1463.352 kb on - strand, within MMJJ_RS07980 at 1463.353 kb on + strand, within MMJJ_RS07980 at 1463.356 kb on - strand, within MMJJ_RS07980
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium bromide 125 mM remove 1,460,264 + MMJJ_RS07960 0.37 +0.3 1,460,512 + MMJJ_RS07960 0.64 -1.9 1,460,740 + MMJJ_RS07960 0.88 -1.0 1,460,872 - +0.9 1,460,873 + -3.2 1,460,873 + -0.8 1,460,875 - -0.2 1,460,917 - MMJJ_RS07965 0.17 -0.0 1,460,918 + MMJJ_RS07965 0.17 +0.9 1,460,920 - MMJJ_RS07965 0.18 +2.0 1,460,950 + MMJJ_RS07965 0.26 +2.0 1,460,953 - MMJJ_RS07965 0.27 +3.9 1,460,955 - MMJJ_RS07965 0.28 -2.6 1,460,956 + MMJJ_RS07965 0.28 -3.0 1,460,956 + MMJJ_RS07965 0.28 +0.0 1,461,040 - MMJJ_RS07965 0.51 -1.3 1,461,051 - MMJJ_RS07965 0.54 -1.9 1,461,051 - MMJJ_RS07965 0.54 +0.4 1,461,074 + MMJJ_RS07965 0.61 -0.2 1,461,207 + +5.4 1,462,053 + MMJJ_RS07970 0.73 -2.0 1,462,093 + MMJJ_RS07970 0.76 -0.7 1,462,246 + MMJJ_RS07970 0.90 -1.5 1,462,299 + +1.3 1,462,327 - -1.3 1,462,345 - -1.3 1,462,364 + -1.3 1,462,388 - -2.2 1,462,392 - -2.0 1,462,392 - +1.7 1,462,393 + -2.2 1,462,412 - -1.0 1,462,412 - +0.4 1,462,413 + -1.3 1,462,431 + -2.0 1,462,447 - -3.5 1,462,451 - -1.7 1,462,451 - +1.3 1,462,454 - -3.8 1,462,454 - +0.7 1,462,455 + -2.2 1,462,499 + MMJJ_RS07975 0.14 -3.6 1,462,510 - MMJJ_RS07975 0.15 -3.6 1,462,545 - MMJJ_RS07975 0.20 -3.3 1,462,572 + MMJJ_RS07975 0.23 +0.1 1,462,603 - MMJJ_RS07975 0.27 -3.6 1,462,604 + MMJJ_RS07975 0.27 +0.3 1,462,651 - MMJJ_RS07975 0.33 -0.7 1,462,652 + MMJJ_RS07975 0.34 -3.5 1,462,680 - MMJJ_RS07975 0.37 +0.3 1,462,681 + MMJJ_RS07975 0.37 -1.5 1,462,754 + MMJJ_RS07975 0.47 -2.9 1,462,764 + MMJJ_RS07975 0.48 -3.2 1,462,784 - MMJJ_RS07975 0.51 +0.3 1,462,786 - MMJJ_RS07975 0.51 -3.0 1,462,821 - MMJJ_RS07975 0.55 -1.8 1,462,821 - MMJJ_RS07975 0.55 -4.3 1,462,846 - MMJJ_RS07975 0.59 -2.9 1,463,059 - MMJJ_RS07975 0.86 -2.4 1,463,060 + MMJJ_RS07975 0.86 -3.3 1,463,060 + MMJJ_RS07975 0.86 -0.8 1,463,060 + MMJJ_RS07975 0.86 -2.9 1,463,234 - -0.8 1,463,352 - MMJJ_RS07980 0.19 -1.0 1,463,353 + MMJJ_RS07980 0.19 -1.9 1,463,356 - MMJJ_RS07980 0.20 +1.3
Or see this region's nucleotide sequence