Experiment: McCas_formate_complete sodium bromide 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04115 and MMJJ_RS04120 are separated by 40 nucleotides MMJJ_RS04120 and MMJJ_RS04125 are separated by 28 nucleotides MMJJ_RS04125 and MMJJ_RS04130 are separated by 231 nucleotides
MMJJ_RS04115: MMJJ_RS04115 - HDIG domain-containing protein, at 763,343 to 764,029
_RS04115
MMJJ_RS04120: MMJJ_RS04120 - amino acid ABC transporter ATP-binding protein, at 764,070 to 764,801
_RS04120
MMJJ_RS04125: MMJJ_RS04125 - tRNA (guanine(10)-N(2))-dimethyltransferase, at 764,830 to 765,951
_RS04125
MMJJ_RS04130: MMJJ_RS04130 - anaerobic ribonucleoside-triphosphate reductase, at 766,183 to 768,495
_RS04130
Position (kb) 764
765
766 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.845 kb on - strand, within MMJJ_RS04115 at 763.846 kb on + strand, within MMJJ_RS04115 at 763.846 kb on + strand, within MMJJ_RS04115 at 763.859 kb on + strand, within MMJJ_RS04115 at 763.867 kb on + strand, within MMJJ_RS04115 at 763.878 kb on - strand, within MMJJ_RS04115 at 763.879 kb on + strand, within MMJJ_RS04115 at 763.894 kb on - strand, within MMJJ_RS04115 at 764.119 kb on - strand at 764.129 kb on + strand at 764.159 kb on + strand, within MMJJ_RS04120 at 764.201 kb on - strand, within MMJJ_RS04120 at 764.240 kb on - strand, within MMJJ_RS04120 at 764.261 kb on - strand, within MMJJ_RS04120 at 764.328 kb on + strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.363 kb on - strand, within MMJJ_RS04120 at 764.387 kb on + strand, within MMJJ_RS04120 at 764.428 kb on - strand, within MMJJ_RS04120 at 764.428 kb on - strand, within MMJJ_RS04120 at 764.429 kb on + strand, within MMJJ_RS04120 at 764.431 kb on - strand, within MMJJ_RS04120 at 764.546 kb on + strand, within MMJJ_RS04120 at 764.546 kb on + strand, within MMJJ_RS04120 at 764.594 kb on + strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.602 kb on - strand, within MMJJ_RS04120 at 764.644 kb on - strand, within MMJJ_RS04120 at 764.741 kb on - strand at 764.741 kb on - strand at 764.742 kb on + strand at 764.751 kb on + strand at 764.751 kb on + strand at 764.795 kb on - strand at 764.795 kb on - strand at 764.796 kb on + strand at 764.799 kb on - strand at 764.799 kb on - strand at 764.799 kb on - strand at 764.800 kb on + strand at 764.800 kb on + strand at 764.802 kb on - strand at 764.815 kb on + strand at 764.820 kb on - strand at 764.820 kb on - strand at 764.820 kb on - strand at 764.925 kb on - strand at 764.931 kb on - strand at 764.962 kb on + strand, within MMJJ_RS04125 at 764.992 kb on + strand, within MMJJ_RS04125 at 765.230 kb on - strand, within MMJJ_RS04125 at 765.231 kb on + strand, within MMJJ_RS04125 at 765.368 kb on - strand, within MMJJ_RS04125 at 765.486 kb on - strand, within MMJJ_RS04125 at 765.490 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.533 kb on + strand, within MMJJ_RS04125 at 765.533 kb on + strand, within MMJJ_RS04125 at 765.562 kb on - strand, within MMJJ_RS04125 at 765.563 kb on + strand, within MMJJ_RS04125 at 765.571 kb on - strand, within MMJJ_RS04125 at 765.572 kb on + strand, within MMJJ_RS04125 at 765.576 kb on - strand, within MMJJ_RS04125 at 765.577 kb on + strand, within MMJJ_RS04125 at 765.580 kb on - strand, within MMJJ_RS04125 at 765.604 kb on - strand, within MMJJ_RS04125 at 765.626 kb on + strand, within MMJJ_RS04125 at 765.650 kb on + strand, within MMJJ_RS04125 at 765.691 kb on + strand, within MMJJ_RS04125 at 765.871 kb on + strand at 765.872 kb on - strand at 765.877 kb on - strand at 765.878 kb on + strand at 765.882 kb on - strand at 765.939 kb on - strand at 765.943 kb on - strand at 765.965 kb on + strand at 765.975 kb on + strand at 765.975 kb on + strand at 765.988 kb on - strand at 765.995 kb on + strand at 765.998 kb on - strand at 765.998 kb on - strand at 765.999 kb on + strand at 766.002 kb on - strand at 766.002 kb on - strand at 766.003 kb on + strand at 766.006 kb on - strand at 766.034 kb on - strand at 766.034 kb on - strand at 766.035 kb on + strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.042 kb on + strand at 766.047 kb on - strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.051 kb on - strand at 766.052 kb on + strand at 766.052 kb on + strand at 766.056 kb on - strand at 766.057 kb on + strand at 766.057 kb on + strand at 766.069 kb on + strand at 766.073 kb on - strand at 766.086 kb on + strand at 766.172 kb on - strand at 766.602 kb on + strand, within MMJJ_RS04130 at 766.830 kb on + strand, within MMJJ_RS04130
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium bromide 125 mM remove 763,845 - MMJJ_RS04115 0.73 -3.5 763,845 - MMJJ_RS04115 0.73 -2.9 763,845 - MMJJ_RS04115 0.73 +2.4 763,846 + MMJJ_RS04115 0.73 +1.1 763,846 + MMJJ_RS04115 0.73 -2.8 763,859 + MMJJ_RS04115 0.75 +3.9 763,867 + MMJJ_RS04115 0.76 +0.0 763,878 - MMJJ_RS04115 0.78 +1.8 763,879 + MMJJ_RS04115 0.78 +2.3 763,894 - MMJJ_RS04115 0.80 +0.8 764,119 - -0.4 764,129 + -2.6 764,159 + MMJJ_RS04120 0.12 +1.8 764,201 - MMJJ_RS04120 0.18 +2.2 764,240 - MMJJ_RS04120 0.23 -2.3 764,261 - MMJJ_RS04120 0.26 +0.9 764,328 + MMJJ_RS04120 0.35 +3.6 764,363 - MMJJ_RS04120 0.40 +0.1 764,363 - MMJJ_RS04120 0.40 +0.5 764,363 - MMJJ_RS04120 0.40 +1.0 764,387 + MMJJ_RS04120 0.43 +0.2 764,428 - MMJJ_RS04120 0.49 +2.5 764,428 - MMJJ_RS04120 0.49 +1.4 764,429 + MMJJ_RS04120 0.49 -1.6 764,431 - MMJJ_RS04120 0.49 -1.3 764,546 + MMJJ_RS04120 0.65 -1.4 764,546 + MMJJ_RS04120 0.65 +0.1 764,594 + MMJJ_RS04120 0.72 -0.3 764,602 - MMJJ_RS04120 0.73 -1.5 764,602 - MMJJ_RS04120 0.73 -1.1 764,602 - MMJJ_RS04120 0.73 +1.9 764,644 - MMJJ_RS04120 0.78 -2.6 764,741 - +1.1 764,741 - -1.3 764,742 + +2.5 764,751 + -2.0 764,751 + -1.6 764,795 - -1.5 764,795 - +0.2 764,796 + +2.4 764,799 - -0.6 764,799 - +2.0 764,799 - -1.1 764,800 + +0.9 764,800 + +0.5 764,802 - -3.1 764,815 + -4.7 764,820 - -1.4 764,820 - +2.1 764,820 - -2.5 764,925 - -2.3 764,931 - +0.1 764,962 + MMJJ_RS04125 0.12 -1.5 764,992 + MMJJ_RS04125 0.14 +0.2 765,230 - MMJJ_RS04125 0.36 -3.0 765,231 + MMJJ_RS04125 0.36 -1.6 765,368 - MMJJ_RS04125 0.48 -1.2 765,486 - MMJJ_RS04125 0.58 +0.1 765,490 - MMJJ_RS04125 0.59 -2.3 765,532 - MMJJ_RS04125 0.63 -0.0 765,532 - MMJJ_RS04125 0.63 -2.3 765,532 - MMJJ_RS04125 0.63 -2.2 765,533 + MMJJ_RS04125 0.63 -1.8 765,533 + MMJJ_RS04125 0.63 -1.5 765,562 - MMJJ_RS04125 0.65 -2.9 765,563 + MMJJ_RS04125 0.65 -0.1 765,571 - MMJJ_RS04125 0.66 -3.1 765,572 + MMJJ_RS04125 0.66 +2.2 765,576 - MMJJ_RS04125 0.66 +1.1 765,577 + MMJJ_RS04125 0.67 -2.2 765,580 - MMJJ_RS04125 0.67 -1.9 765,604 - MMJJ_RS04125 0.69 -3.3 765,626 + MMJJ_RS04125 0.71 -2.0 765,650 + MMJJ_RS04125 0.73 -0.2 765,691 + MMJJ_RS04125 0.77 -1.5 765,871 + +1.3 765,872 - +0.6 765,877 - -2.1 765,878 + -2.6 765,882 - +0.7 765,939 - +0.1 765,943 - +0.7 765,965 + +0.3 765,975 + +1.4 765,975 + -1.1 765,988 - -3.7 765,995 + +1.9 765,998 - -0.1 765,998 - -3.1 765,999 + -0.1 766,002 - +0.9 766,002 - +0.4 766,003 + +0.1 766,006 - +2.6 766,034 - -2.4 766,034 - -2.0 766,035 + -2.3 766,036 - +0.6 766,036 - -2.2 766,036 - -3.4 766,036 - -2.4 766,036 - -0.6 766,036 - -1.6 766,036 - -1.4 766,036 - -1.0 766,037 + -1.8 766,037 + -1.2 766,037 + -1.6 766,037 + -1.2 766,037 + -1.4 766,038 - -0.6 766,038 - -2.1 766,038 - -0.6 766,038 - -2.4 766,038 - -0.6 766,039 + -2.2 766,039 + -1.6 766,039 + -2.4 766,039 + +0.8 766,039 + -1.4 766,039 + -1.3 766,039 + -2.6 766,042 + -0.7 766,047 - -0.2 766,048 + -2.2 766,048 + -1.5 766,048 + -2.2 766,048 + -2.4 766,048 + -1.2 766,051 - -3.6 766,052 + -1.2 766,052 + -1.6 766,056 - +0.4 766,057 + +0.4 766,057 + -1.2 766,069 + -1.4 766,073 - -0.8 766,086 + +1.4 766,172 - +0.4 766,602 + MMJJ_RS04130 0.18 -0.2 766,830 + MMJJ_RS04130 0.28 -0.2
Or see this region's nucleotide sequence