Experiment: McCas_formate_complete sodium bromide 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01580 and MMJJ_RS01585 are separated by 204 nucleotides MMJJ_RS01585 and MMJJ_RS01590 are separated by 208 nucleotides MMJJ_RS01590 and MMJJ_RS01595 are separated by 42 nucleotides
MMJJ_RS01580: MMJJ_RS01580 - phosphoadenosine phosphosulfate reductase family protein, at 307,121 to 307,996
_RS01580
MMJJ_RS01585: MMJJ_RS01585 - type I restriction endonuclease, at 308,201 to 309,325
_RS01585
MMJJ_RS01590: MMJJ_RS01590 - adenine phosphoribosyltransferase, at 309,534 to 310,052
_RS01590
MMJJ_RS01595: MMJJ_RS01595 - U32 family peptidase, at 310,095 to 311,306
_RS01595
Position (kb) 308
309
310 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 307.241 kb on + strand, within MMJJ_RS01580 at 307.316 kb on - strand, within MMJJ_RS01580 at 307.374 kb on + strand, within MMJJ_RS01580 at 307.381 kb on - strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.383 kb on - strand, within MMJJ_RS01580 at 307.384 kb on + strand, within MMJJ_RS01580 at 307.384 kb on + strand, within MMJJ_RS01580 at 307.387 kb on - strand, within MMJJ_RS01580 at 307.388 kb on + strand, within MMJJ_RS01580 at 307.388 kb on + strand, within MMJJ_RS01580 at 307.388 kb on + strand, within MMJJ_RS01580 at 307.405 kb on - strand, within MMJJ_RS01580 at 307.406 kb on + strand, within MMJJ_RS01580 at 307.407 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.430 kb on + strand, within MMJJ_RS01580 at 307.430 kb on + strand, within MMJJ_RS01580 at 307.430 kb on + strand, within MMJJ_RS01580 at 307.540 kb on - strand, within MMJJ_RS01580 at 307.541 kb on + strand, within MMJJ_RS01580 at 307.557 kb on + strand, within MMJJ_RS01580 at 307.661 kb on + strand, within MMJJ_RS01580 at 307.669 kb on - strand, within MMJJ_RS01580 at 307.706 kb on - strand, within MMJJ_RS01580 at 307.713 kb on + strand, within MMJJ_RS01580 at 307.747 kb on + strand, within MMJJ_RS01580 at 307.798 kb on + strand, within MMJJ_RS01580 at 308.004 kb on - strand at 308.010 kb on - strand at 308.011 kb on + strand at 308.011 kb on + strand at 308.013 kb on - strand at 308.013 kb on - strand at 308.014 kb on + strand at 308.014 kb on + strand at 308.018 kb on + strand at 308.018 kb on + strand at 308.018 kb on + strand at 308.022 kb on + strand at 308.031 kb on + strand at 308.031 kb on + strand at 308.034 kb on - strand at 308.035 kb on + strand at 308.035 kb on + strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.039 kb on + strand at 308.039 kb on + strand at 308.042 kb on - strand at 308.042 kb on - strand at 308.043 kb on + strand at 308.043 kb on + strand at 308.052 kb on + strand at 308.058 kb on + strand at 308.064 kb on + strand at 308.110 kb on - strand at 308.111 kb on + strand at 308.111 kb on + strand at 308.112 kb on - strand at 308.113 kb on + strand at 308.123 kb on + strand at 308.138 kb on - strand at 308.140 kb on - strand at 308.141 kb on + strand at 308.141 kb on + strand at 308.169 kb on - strand at 308.170 kb on + strand at 308.170 kb on + strand at 308.173 kb on - strand at 308.173 kb on - strand at 308.179 kb on - strand at 308.180 kb on + strand at 308.209 kb on - strand at 308.210 kb on + strand at 308.236 kb on + strand at 308.259 kb on + strand at 308.266 kb on - strand at 308.278 kb on - strand at 308.295 kb on - strand at 308.328 kb on - strand, within MMJJ_RS01585 at 308.329 kb on + strand, within MMJJ_RS01585 at 308.332 kb on - strand, within MMJJ_RS01585 at 308.416 kb on - strand, within MMJJ_RS01585 at 308.434 kb on - strand, within MMJJ_RS01585 at 308.443 kb on - strand, within MMJJ_RS01585 at 308.452 kb on - strand, within MMJJ_RS01585 at 308.493 kb on + strand, within MMJJ_RS01585 at 308.493 kb on + strand, within MMJJ_RS01585 at 308.530 kb on + strand, within MMJJ_RS01585 at 308.533 kb on - strand, within MMJJ_RS01585 at 308.534 kb on + strand, within MMJJ_RS01585 at 308.547 kb on + strand, within MMJJ_RS01585 at 308.547 kb on + strand, within MMJJ_RS01585 at 308.548 kb on - strand, within MMJJ_RS01585 at 308.579 kb on + strand, within MMJJ_RS01585 at 308.793 kb on - strand, within MMJJ_RS01585 at 308.793 kb on - strand, within MMJJ_RS01585 at 308.860 kb on - strand, within MMJJ_RS01585 at 308.860 kb on - strand, within MMJJ_RS01585 at 308.861 kb on + strand, within MMJJ_RS01585 at 308.861 kb on + strand, within MMJJ_RS01585 at 308.864 kb on + strand, within MMJJ_RS01585 at 308.871 kb on - strand, within MMJJ_RS01585 at 308.875 kb on - strand, within MMJJ_RS01585 at 308.884 kb on - strand, within MMJJ_RS01585 at 308.884 kb on - strand, within MMJJ_RS01585 at 308.897 kb on + strand, within MMJJ_RS01585 at 308.926 kb on - strand, within MMJJ_RS01585 at 308.927 kb on + strand, within MMJJ_RS01585 at 308.985 kb on - strand, within MMJJ_RS01585 at 308.986 kb on + strand, within MMJJ_RS01585 at 308.986 kb on + strand, within MMJJ_RS01585 at 308.990 kb on + strand, within MMJJ_RS01585 at 308.990 kb on + strand, within MMJJ_RS01585 at 308.990 kb on + strand, within MMJJ_RS01585 at 309.014 kb on - strand, within MMJJ_RS01585 at 309.014 kb on - strand, within MMJJ_RS01585 at 309.033 kb on - strand, within MMJJ_RS01585 at 309.033 kb on - strand at 309.044 kb on - strand, within MMJJ_RS01585 at 309.044 kb on - strand, within MMJJ_RS01585 at 309.045 kb on + strand, within MMJJ_RS01585 at 309.055 kb on - strand, within MMJJ_RS01585 at 309.056 kb on + strand, within MMJJ_RS01585 at 309.056 kb on + strand, within MMJJ_RS01585 at 309.066 kb on - strand, within MMJJ_RS01585 at 309.066 kb on - strand, within MMJJ_RS01585 at 309.067 kb on + strand, within MMJJ_RS01585 at 309.067 kb on + strand, within MMJJ_RS01585 at 309.073 kb on - strand, within MMJJ_RS01585 at 309.074 kb on + strand, within MMJJ_RS01585 at 309.089 kb on - strand, within MMJJ_RS01585 at 309.090 kb on + strand, within MMJJ_RS01585 at 309.099 kb on - strand, within MMJJ_RS01585 at 309.099 kb on - strand, within MMJJ_RS01585 at 309.099 kb on - strand, within MMJJ_RS01585 at 309.100 kb on + strand, within MMJJ_RS01585 at 309.101 kb on - strand, within MMJJ_RS01585 at 309.102 kb on + strand, within MMJJ_RS01585 at 309.102 kb on + strand, within MMJJ_RS01585 at 309.115 kb on + strand, within MMJJ_RS01585 at 309.137 kb on + strand, within MMJJ_RS01585 at 309.141 kb on - strand, within MMJJ_RS01585 at 309.142 kb on + strand, within MMJJ_RS01585 at 309.154 kb on - strand, within MMJJ_RS01585 at 309.154 kb on - strand, within MMJJ_RS01585 at 309.155 kb on + strand, within MMJJ_RS01585 at 309.160 kb on - strand, within MMJJ_RS01585 at 309.160 kb on - strand, within MMJJ_RS01585 at 309.160 kb on - strand, within MMJJ_RS01585 at 309.161 kb on + strand, within MMJJ_RS01585 at 309.161 kb on + strand, within MMJJ_RS01585 at 309.161 kb on + strand, within MMJJ_RS01585 at 309.161 kb on + strand, within MMJJ_RS01585 at 309.161 kb on + strand, within MMJJ_RS01585 at 309.163 kb on - strand, within MMJJ_RS01585 at 309.164 kb on + strand, within MMJJ_RS01585 at 309.164 kb on + strand, within MMJJ_RS01585 at 309.190 kb on - strand, within MMJJ_RS01585 at 309.202 kb on - strand, within MMJJ_RS01585 at 309.244 kb on - strand at 309.252 kb on - strand at 309.253 kb on + strand at 309.253 kb on + strand at 309.259 kb on - strand at 309.295 kb on + strand at 309.301 kb on + strand at 309.323 kb on - strand at 309.351 kb on + strand at 309.356 kb on - strand at 309.383 kb on + strand at 309.401 kb on - strand at 309.406 kb on - strand at 309.406 kb on - strand at 309.406 kb on - strand at 309.406 kb on - strand at 309.409 kb on - strand at 309.409 kb on - strand at 309.409 kb on - strand at 309.409 kb on - strand at 309.410 kb on + strand at 309.410 kb on + strand at 309.411 kb on - strand at 309.411 kb on - strand at 309.411 kb on - strand at 309.411 kb on - strand at 309.411 kb on - strand at 309.411 kb on - strand at 309.412 kb on + strand at 309.412 kb on + strand at 309.412 kb on + strand at 309.413 kb on - strand at 309.414 kb on + strand at 309.414 kb on + strand at 309.414 kb on + strand at 309.414 kb on + strand at 309.432 kb on + strand at 309.432 kb on + strand at 309.434 kb on - strand at 309.435 kb on + strand at 309.436 kb on - strand at 309.437 kb on + strand at 309.437 kb on + strand at 309.438 kb on - strand at 309.438 kb on - strand at 309.438 kb on - strand at 309.438 kb on - strand at 309.439 kb on + strand at 309.439 kb on + strand at 309.448 kb on - strand at 309.449 kb on + strand at 309.453 kb on - strand at 309.489 kb on - strand at 309.494 kb on - strand at 309.502 kb on + strand at 309.541 kb on - strand at 309.563 kb on + strand at 309.613 kb on - strand, within MMJJ_RS01590 at 309.917 kb on + strand, within MMJJ_RS01590 at 310.025 kb on + strand at 310.037 kb on - strand at 310.073 kb on - strand at 310.276 kb on + strand, within MMJJ_RS01595 at 310.279 kb on - strand, within MMJJ_RS01595 at 310.315 kb on - strand, within MMJJ_RS01595
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium bromide 125 mM remove 307,241 + MMJJ_RS01580 0.14 +1.0 307,316 - MMJJ_RS01580 0.22 -1.3 307,374 + MMJJ_RS01580 0.29 -0.6 307,381 - MMJJ_RS01580 0.30 -0.4 307,382 + MMJJ_RS01580 0.30 +0.7 307,382 + MMJJ_RS01580 0.30 +1.3 307,382 + MMJJ_RS01580 0.30 -0.2 307,382 + MMJJ_RS01580 0.30 +1.4 307,382 + MMJJ_RS01580 0.30 +0.2 307,383 - MMJJ_RS01580 0.30 +1.6 307,384 + MMJJ_RS01580 0.30 -3.6 307,384 + MMJJ_RS01580 0.30 +3.2 307,387 - MMJJ_RS01580 0.30 -0.2 307,388 + MMJJ_RS01580 0.30 -2.7 307,388 + MMJJ_RS01580 0.30 -2.8 307,388 + MMJJ_RS01580 0.30 +1.4 307,405 - MMJJ_RS01580 0.32 -2.3 307,406 + MMJJ_RS01580 0.33 +0.2 307,407 - MMJJ_RS01580 0.33 -3.4 307,429 - MMJJ_RS01580 0.35 -1.5 307,429 - MMJJ_RS01580 0.35 +1.4 307,429 - MMJJ_RS01580 0.35 -0.6 307,429 - MMJJ_RS01580 0.35 +1.0 307,429 - MMJJ_RS01580 0.35 -1.6 307,430 + MMJJ_RS01580 0.35 -0.6 307,430 + MMJJ_RS01580 0.35 +0.7 307,430 + MMJJ_RS01580 0.35 -1.2 307,540 - MMJJ_RS01580 0.48 +0.4 307,541 + MMJJ_RS01580 0.48 +3.0 307,557 + MMJJ_RS01580 0.50 -0.7 307,661 + MMJJ_RS01580 0.62 -2.7 307,669 - MMJJ_RS01580 0.63 -2.4 307,706 - MMJJ_RS01580 0.67 +1.3 307,713 + MMJJ_RS01580 0.68 -2.1 307,747 + MMJJ_RS01580 0.71 -5.0 307,798 + MMJJ_RS01580 0.77 -0.1 308,004 - -1.6 308,010 - +0.0 308,011 + +2.1 308,011 + -2.0 308,013 - +1.7 308,013 - -0.3 308,014 + +3.8 308,014 + +2.5 308,018 + +0.9 308,018 + +0.3 308,018 + +0.6 308,022 + -1.9 308,031 + -0.4 308,031 + -1.9 308,034 - -1.8 308,035 + -4.2 308,035 + +0.6 308,038 - -1.4 308,038 - -0.8 308,038 - +0.2 308,038 - -2.5 308,038 - -1.1 308,038 - -1.4 308,039 + -1.7 308,039 + +1.7 308,042 - -3.4 308,042 - -2.3 308,043 + -0.8 308,043 + -3.5 308,052 + +0.8 308,058 + +0.9 308,064 + +1.4 308,110 - -0.3 308,111 + -2.8 308,111 + +1.1 308,112 - +1.6 308,113 + +0.8 308,123 + +0.5 308,138 - +1.6 308,140 - -1.2 308,141 + -3.8 308,141 + -2.0 308,169 - +2.5 308,170 + -4.8 308,170 + -0.0 308,173 - -2.3 308,173 - -2.4 308,179 - +2.2 308,180 + -5.0 308,209 - -2.5 308,210 + -1.4 308,236 + -1.2 308,259 + +0.3 308,266 - +3.0 308,278 - -1.5 308,295 - +2.3 308,328 - MMJJ_RS01585 0.11 -2.2 308,329 + MMJJ_RS01585 0.11 -1.6 308,332 - MMJJ_RS01585 0.12 +1.2 308,416 - MMJJ_RS01585 0.19 -1.4 308,434 - MMJJ_RS01585 0.21 +1.2 308,443 - MMJJ_RS01585 0.22 -0.3 308,452 - MMJJ_RS01585 0.22 +0.1 308,493 + MMJJ_RS01585 0.26 -0.2 308,493 + MMJJ_RS01585 0.26 -2.7 308,530 + MMJJ_RS01585 0.29 -0.2 308,533 - MMJJ_RS01585 0.30 +1.9 308,534 + MMJJ_RS01585 0.30 -2.7 308,547 + MMJJ_RS01585 0.31 -2.0 308,547 + MMJJ_RS01585 0.31 +1.5 308,548 - MMJJ_RS01585 0.31 +0.2 308,579 + MMJJ_RS01585 0.34 -3.9 308,793 - MMJJ_RS01585 0.53 -2.2 308,793 - MMJJ_RS01585 0.53 -3.0 308,860 - MMJJ_RS01585 0.59 +0.9 308,860 - MMJJ_RS01585 0.59 +0.3 308,861 + MMJJ_RS01585 0.59 -2.7 308,861 + MMJJ_RS01585 0.59 -3.0 308,864 + MMJJ_RS01585 0.59 -3.4 308,871 - MMJJ_RS01585 0.60 +2.9 308,875 - MMJJ_RS01585 0.60 +0.1 308,884 - MMJJ_RS01585 0.61 +0.3 308,884 - MMJJ_RS01585 0.61 +0.2 308,897 + MMJJ_RS01585 0.62 +1.8 308,926 - MMJJ_RS01585 0.64 -2.8 308,927 + MMJJ_RS01585 0.65 +0.3 308,985 - MMJJ_RS01585 0.70 -3.6 308,986 + MMJJ_RS01585 0.70 +2.2 308,986 + MMJJ_RS01585 0.70 +2.8 308,990 + MMJJ_RS01585 0.70 -2.2 308,990 + MMJJ_RS01585 0.70 -1.6 308,990 + MMJJ_RS01585 0.70 -0.4 309,014 - MMJJ_RS01585 0.72 -0.8 309,014 - MMJJ_RS01585 0.72 -0.4 309,033 - MMJJ_RS01585 0.74 -1.6 309,033 - -1.2 309,044 - MMJJ_RS01585 0.75 -1.4 309,044 - MMJJ_RS01585 0.75 +3.5 309,045 + MMJJ_RS01585 0.75 +1.2 309,055 - MMJJ_RS01585 0.76 +1.3 309,056 + MMJJ_RS01585 0.76 -2.9 309,056 + MMJJ_RS01585 0.76 +1.6 309,066 - MMJJ_RS01585 0.77 +4.2 309,066 - MMJJ_RS01585 0.77 +0.0 309,067 + MMJJ_RS01585 0.77 -0.3 309,067 + MMJJ_RS01585 0.77 +1.4 309,073 - MMJJ_RS01585 0.78 -1.2 309,074 + MMJJ_RS01585 0.78 -3.3 309,089 - MMJJ_RS01585 0.79 +0.6 309,090 + MMJJ_RS01585 0.79 -3.4 309,099 - MMJJ_RS01585 0.80 -0.8 309,099 - MMJJ_RS01585 0.80 +1.9 309,099 - MMJJ_RS01585 0.80 +1.6 309,100 + MMJJ_RS01585 0.80 +2.7 309,101 - MMJJ_RS01585 0.80 -1.2 309,102 + MMJJ_RS01585 0.80 -3.1 309,102 + MMJJ_RS01585 0.80 +2.5 309,115 + MMJJ_RS01585 0.81 +1.2 309,137 + MMJJ_RS01585 0.83 +1.2 309,141 - MMJJ_RS01585 0.84 -2.6 309,142 + MMJJ_RS01585 0.84 -0.3 309,154 - MMJJ_RS01585 0.85 -1.4 309,154 - MMJJ_RS01585 0.85 -0.1 309,155 + MMJJ_RS01585 0.85 -2.4 309,160 - MMJJ_RS01585 0.85 -1.8 309,160 - MMJJ_RS01585 0.85 +1.8 309,160 - MMJJ_RS01585 0.85 -2.4 309,161 + MMJJ_RS01585 0.85 +1.7 309,161 + MMJJ_RS01585 0.85 -1.5 309,161 + MMJJ_RS01585 0.85 -0.4 309,161 + MMJJ_RS01585 0.85 +2.0 309,161 + MMJJ_RS01585 0.85 -0.8 309,163 - MMJJ_RS01585 0.86 -0.4 309,164 + MMJJ_RS01585 0.86 -1.2 309,164 + MMJJ_RS01585 0.86 -0.2 309,190 - MMJJ_RS01585 0.88 -3.0 309,202 - MMJJ_RS01585 0.89 -3.3 309,244 - -4.0 309,252 - +0.0 309,253 + -3.4 309,253 + +0.0 309,259 - -1.3 309,295 + +0.3 309,301 + +0.2 309,323 - -0.6 309,351 + +1.2 309,356 - +2.4 309,383 + -0.4 309,401 - +2.5 309,406 - -2.5 309,406 - -2.4 309,406 - +0.1 309,406 - +0.5 309,409 - -1.2 309,409 - +0.9 309,409 - -1.2 309,409 - +3.0 309,410 + -1.7 309,410 + +3.6 309,411 - +0.8 309,411 - +1.5 309,411 - +1.2 309,411 - -1.5 309,411 - +1.2 309,411 - -2.2 309,412 + +3.1 309,412 + +1.4 309,412 + +3.1 309,413 - +3.0 309,414 + +1.4 309,414 + +1.2 309,414 + -1.4 309,414 + -0.1 309,432 + -0.6 309,432 + +2.7 309,434 - -1.4 309,435 + +0.6 309,436 - +2.8 309,437 + -2.9 309,437 + -0.6 309,438 - -0.4 309,438 - -0.7 309,438 - -0.2 309,438 - +1.8 309,439 + +1.2 309,439 + +0.7 309,448 - +1.1 309,449 + -0.7 309,453 - -2.6 309,489 - -3.8 309,494 - -2.3 309,502 + -0.1 309,541 - -1.8 309,563 + -1.8 309,613 - MMJJ_RS01590 0.15 +0.4 309,917 + MMJJ_RS01590 0.74 +3.7 310,025 + +2.1 310,037 - -1.4 310,073 - +3.6 310,276 + MMJJ_RS01595 0.15 +1.0 310,279 - MMJJ_RS01595 0.15 +2.3 310,315 - MMJJ_RS01595 0.18 +2.2
Or see this region's nucleotide sequence