Experiment: McCas_formate_complete Novobiocin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08500 and MMJJ_RS08505 are separated by 17 nucleotides MMJJ_RS08505 and MMJJ_RS08510 are separated by 186 nucleotides MMJJ_RS08510 and MMJJ_RS08515 are separated by 73 nucleotides MMJJ_RS08515 and MMJJ_RS08520 are separated by 114 nucleotides
MMJJ_RS08500: MMJJ_RS08500 - L-sulfolactate dehydrogenase, at 1,563,906 to 1,564,949
_RS08500
MMJJ_RS08505: MMJJ_RS08505 - tRNA-intron lyase, at 1,564,967 to 1,565,500
_RS08505
MMJJ_RS08510: MMJJ_RS08510 - peptide chain release factor aRF-1, at 1,565,687 to 1,566,946
_RS08510
MMJJ_RS08515: MMJJ_RS08515 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,567,020 to 1,567,418
_RS08515
MMJJ_RS08520: MMJJ_RS08520 - peptidylprolyl isomerase, at 1,567,533 to 1,568,021
_RS08520
Position (kb)
1565
1566
1567 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1564.860 kb on + strand at 1564.862 kb on - strand at 1564.863 kb on + strand at 1564.888 kb on - strand at 1564.889 kb on + strand at 1564.889 kb on + strand at 1564.934 kb on - strand at 1564.938 kb on - strand at 1564.938 kb on - strand at 1564.938 kb on - strand at 1564.938 kb on - strand at 1564.938 kb on - strand at 1564.939 kb on + strand at 1564.939 kb on + strand at 1564.939 kb on + strand at 1564.939 kb on + strand at 1564.939 kb on + strand at 1564.939 kb on + strand at 1564.940 kb on - strand at 1564.940 kb on - strand at 1564.941 kb on + strand at 1564.941 kb on + strand at 1564.941 kb on + strand at 1564.941 kb on + strand at 1564.965 kb on + strand at 1565.230 kb on + strand, within MMJJ_RS08505 at 1565.282 kb on - strand, within MMJJ_RS08505 at 1565.423 kb on - strand, within MMJJ_RS08505 at 1565.501 kb on + strand at 1565.519 kb on - strand at 1565.544 kb on - strand at 1565.544 kb on - strand at 1565.544 kb on - strand at 1565.544 kb on - strand at 1565.544 kb on - strand at 1565.544 kb on - strand at 1565.545 kb on + strand at 1565.545 kb on + strand at 1565.545 kb on + strand at 1565.545 kb on + strand at 1565.545 kb on + strand at 1565.545 kb on + strand at 1565.548 kb on - strand at 1565.548 kb on - strand at 1565.549 kb on + strand at 1565.549 kb on + strand at 1565.549 kb on + strand at 1565.551 kb on - strand at 1565.551 kb on - strand at 1565.552 kb on + strand at 1565.553 kb on - strand at 1565.553 kb on - strand at 1565.554 kb on + strand at 1565.554 kb on + strand at 1565.554 kb on + strand at 1565.555 kb on - strand at 1565.555 kb on - strand at 1565.555 kb on - strand at 1565.556 kb on + strand at 1565.556 kb on + strand at 1565.556 kb on + strand at 1565.556 kb on + strand at 1565.556 kb on + strand at 1565.572 kb on - strand at 1565.575 kb on + strand at 1566.838 kb on - strand at 1566.944 kb on - strand at 1566.944 kb on - strand at 1566.964 kb on + strand at 1566.980 kb on - strand at 1566.981 kb on + strand at 1566.987 kb on + strand at 1566.991 kb on + strand at 1567.001 kb on + strand at 1567.020 kb on + strand at 1567.088 kb on + strand, within MMJJ_RS08515 at 1567.148 kb on - strand, within MMJJ_RS08515 at 1567.165 kb on - strand, within MMJJ_RS08515 at 1567.165 kb on - strand, within MMJJ_RS08515 at 1567.166 kb on + strand, within MMJJ_RS08515 at 1567.252 kb on + strand, within MMJJ_RS08515 at 1567.252 kb on + strand, within MMJJ_RS08515 at 1567.315 kb on - strand, within MMJJ_RS08515 at 1567.316 kb on + strand, within MMJJ_RS08515 at 1567.421 kb on - strand at 1567.421 kb on - strand at 1567.421 kb on - strand at 1567.422 kb on + strand at 1567.442 kb on + strand at 1567.449 kb on + strand at 1567.454 kb on + strand at 1567.573 kb on - strand at 1567.579 kb on - strand at 1567.579 kb on - strand at 1567.666 kb on - strand, within MMJJ_RS08520 at 1567.808 kb on + strand, within MMJJ_RS08520 at 1567.835 kb on + strand, within MMJJ_RS08520
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Novobiocin 0.0125 mM remove 1,564,860 + -0.5 1,564,862 - -1.5 1,564,863 + -4.1 1,564,888 - -3.3 1,564,889 + -0.5 1,564,889 + -1.2 1,564,934 - -2.7 1,564,938 - -0.3 1,564,938 - -1.8 1,564,938 - -2.7 1,564,938 - -0.5 1,564,938 - -1.5 1,564,939 + -0.5 1,564,939 + -1.2 1,564,939 + -1.5 1,564,939 + -0.3 1,564,939 + -1.5 1,564,939 + -1.9 1,564,940 - -1.5 1,564,940 - -3.8 1,564,941 + -0.8 1,564,941 + -2.9 1,564,941 + -3.4 1,564,941 + -0.9 1,564,965 + -0.5 1,565,230 + MMJJ_RS08505 0.49 -1.5 1,565,282 - MMJJ_RS08505 0.59 -1.2 1,565,423 - MMJJ_RS08505 0.85 +0.1 1,565,501 + -0.5 1,565,519 - +1.7 1,565,544 - -3.2 1,565,544 - -1.5 1,565,544 - -2.6 1,565,544 - +0.1 1,565,544 - +0.9 1,565,544 - -0.3 1,565,545 + -0.8 1,565,545 + +0.1 1,565,545 + -0.0 1,565,545 + +1.1 1,565,545 + +0.1 1,565,545 + -3.4 1,565,548 - -0.7 1,565,548 - +1.3 1,565,549 + -1.9 1,565,549 + -1.2 1,565,549 + -1.5 1,565,551 - +1.6 1,565,551 - -0.9 1,565,552 + -2.2 1,565,553 - -2.4 1,565,553 - +0.6 1,565,554 + -2.7 1,565,554 + -1.9 1,565,554 + -0.5 1,565,555 - -1.6 1,565,555 - -1.9 1,565,555 - -4.2 1,565,556 + +2.8 1,565,556 + -4.3 1,565,556 + -1.7 1,565,556 + +1.9 1,565,556 + -0.9 1,565,572 - +1.5 1,565,575 + -0.5 1,566,838 - -0.5 1,566,944 - -2.6 1,566,944 - +0.7 1,566,964 + -1.3 1,566,980 - -2.6 1,566,981 + -1.5 1,566,987 + +0.1 1,566,991 + -1.4 1,567,001 + -4.0 1,567,020 + -0.3 1,567,088 + MMJJ_RS08515 0.17 -3.7 1,567,148 - MMJJ_RS08515 0.32 -4.6 1,567,165 - MMJJ_RS08515 0.36 -2.2 1,567,165 - MMJJ_RS08515 0.36 -0.0 1,567,166 + MMJJ_RS08515 0.37 +1.4 1,567,252 + MMJJ_RS08515 0.58 -2.4 1,567,252 + MMJJ_RS08515 0.58 -1.1 1,567,315 - MMJJ_RS08515 0.74 -1.5 1,567,316 + MMJJ_RS08515 0.74 +1.0 1,567,421 - -0.9 1,567,421 - -2.0 1,567,421 - +1.1 1,567,422 + +1.0 1,567,442 + -2.6 1,567,449 + -0.7 1,567,454 + +4.4 1,567,573 - -3.6 1,567,579 - +0.5 1,567,579 - +0.1 1,567,666 - MMJJ_RS08520 0.27 +1.0 1,567,808 + MMJJ_RS08520 0.56 +2.4 1,567,835 + MMJJ_RS08520 0.62 -1.3
Or see this region's nucleotide sequence