Experiment: McCas_formate_complete Novobiocin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01835 and MMJJ_RS01840 are separated by 213 nucleotides MMJJ_RS01840 and MMJJ_RS01845 are separated by 96 nucleotides MMJJ_RS01845 and MMJJ_RS09265 are separated by 80 nucleotides
MMJJ_RS01835: MMJJ_RS01835 - tRNA pseudouridine(13) synthase TruD, at 351,867 to 353,159
_RS01835
MMJJ_RS01840: MMJJ_RS01840 - flavodoxin family protein, at 353,373 to 353,948
_RS01840
MMJJ_RS01845: MMJJ_RS01845 - acetolactate decarboxylase, at 354,045 to 354,845
_RS01845
MMJJ_RS09265: MMJJ_RS09265 - hypothetical protein, at 354,926 to 355,072
_RS09265
Position (kb) 353
354 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 352.432 kb on - strand, within MMJJ_RS01835 at 352.472 kb on + strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.483 kb on + strand, within MMJJ_RS01835 at 352.486 kb on - strand, within MMJJ_RS01835 at 352.486 kb on - strand, within MMJJ_RS01835 at 352.487 kb on + strand, within MMJJ_RS01835 at 352.489 kb on - strand, within MMJJ_RS01835 at 352.489 kb on - strand, within MMJJ_RS01835 at 352.490 kb on + strand, within MMJJ_RS01835 at 352.495 kb on + strand, within MMJJ_RS01835 at 352.539 kb on + strand, within MMJJ_RS01835 at 352.558 kb on + strand, within MMJJ_RS01835 at 352.558 kb on + strand, within MMJJ_RS01835 at 352.558 kb on + strand, within MMJJ_RS01835 at 352.584 kb on - strand, within MMJJ_RS01835 at 352.590 kb on - strand, within MMJJ_RS01835 at 352.590 kb on - strand, within MMJJ_RS01835 at 352.590 kb on - strand, within MMJJ_RS01835 at 352.591 kb on + strand, within MMJJ_RS01835 at 352.591 kb on + strand, within MMJJ_RS01835 at 352.606 kb on - strand, within MMJJ_RS01835 at 352.607 kb on + strand, within MMJJ_RS01835 at 352.686 kb on + strand, within MMJJ_RS01835 at 352.722 kb on - strand, within MMJJ_RS01835 at 352.723 kb on + strand at 352.742 kb on - strand, within MMJJ_RS01835 at 352.806 kb on - strand, within MMJJ_RS01835 at 352.806 kb on - strand, within MMJJ_RS01835 at 352.806 kb on - strand, within MMJJ_RS01835 at 352.807 kb on + strand, within MMJJ_RS01835 at 352.807 kb on + strand, within MMJJ_RS01835 at 352.807 kb on + strand, within MMJJ_RS01835 at 352.811 kb on + strand, within MMJJ_RS01835 at 352.811 kb on + strand, within MMJJ_RS01835 at 352.858 kb on - strand, within MMJJ_RS01835 at 352.868 kb on - strand, within MMJJ_RS01835 at 352.869 kb on + strand, within MMJJ_RS01835 at 352.869 kb on + strand, within MMJJ_RS01835 at 352.870 kb on - strand at 352.871 kb on + strand, within MMJJ_RS01835 at 352.913 kb on - strand, within MMJJ_RS01835 at 352.913 kb on - strand, within MMJJ_RS01835 at 352.914 kb on + strand, within MMJJ_RS01835 at 353.038 kb on - strand at 353.038 kb on - strand at 353.049 kb on - strand at 353.050 kb on + strand at 353.057 kb on - strand at 353.096 kb on - strand at 353.166 kb on - strand at 353.166 kb on - strand at 353.166 kb on - strand at 353.166 kb on - strand at 353.167 kb on + strand at 353.167 kb on + strand at 353.167 kb on + strand at 353.167 kb on + strand at 353.167 kb on + strand at 353.168 kb on - strand at 353.168 kb on - strand at 353.168 kb on - strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.169 kb on + strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.170 kb on - strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.171 kb on + strand at 353.172 kb on - strand at 353.172 kb on - strand at 353.172 kb on - strand at 353.172 kb on - strand at 353.173 kb on + strand at 353.173 kb on + strand at 353.190 kb on - strand at 353.190 kb on - strand at 353.190 kb on - strand at 353.191 kb on + strand at 353.235 kb on + strand at 353.235 kb on + strand at 353.235 kb on + strand at 353.293 kb on - strand at 353.293 kb on - strand at 353.294 kb on + strand at 353.294 kb on + strand at 353.298 kb on + strand at 353.302 kb on - strand at 353.430 kb on + strand at 353.433 kb on - strand, within MMJJ_RS01840 at 353.539 kb on + strand, within MMJJ_RS01840 at 353.742 kb on + strand, within MMJJ_RS01840 at 353.745 kb on - strand, within MMJJ_RS01840 at 353.745 kb on - strand at 353.748 kb on - strand, within MMJJ_RS01840 at 353.749 kb on + strand, within MMJJ_RS01840 at 353.750 kb on - strand, within MMJJ_RS01840 at 353.751 kb on + strand, within MMJJ_RS01840 at 353.771 kb on - strand, within MMJJ_RS01840 at 353.772 kb on + strand, within MMJJ_RS01840 at 353.778 kb on - strand, within MMJJ_RS01840 at 353.797 kb on - strand, within MMJJ_RS01840 at 353.797 kb on - strand, within MMJJ_RS01840 at 353.797 kb on - strand, within MMJJ_RS01840 at 353.798 kb on + strand, within MMJJ_RS01840 at 353.807 kb on - strand, within MMJJ_RS01840 at 353.807 kb on - strand, within MMJJ_RS01840 at 353.807 kb on - strand, within MMJJ_RS01840 at 353.808 kb on + strand, within MMJJ_RS01840 at 353.838 kb on - strand, within MMJJ_RS01840 at 353.838 kb on - strand, within MMJJ_RS01840 at 353.838 kb on - strand, within MMJJ_RS01840 at 353.838 kb on - strand, within MMJJ_RS01840 at 353.838 kb on - strand, within MMJJ_RS01840 at 353.839 kb on + strand, within MMJJ_RS01840 at 353.839 kb on + strand, within MMJJ_RS01840 at 353.839 kb on + strand, within MMJJ_RS01840 at 353.839 kb on + strand, within MMJJ_RS01840 at 353.839 kb on + strand, within MMJJ_RS01840 at 353.839 kb on + strand, within MMJJ_RS01840 at 353.850 kb on + strand, within MMJJ_RS01840 at 353.852 kb on - strand, within MMJJ_RS01840 at 353.853 kb on + strand, within MMJJ_RS01840 at 353.886 kb on + strand, within MMJJ_RS01840 at 353.930 kb on - strand at 353.966 kb on - strand at 353.967 kb on + strand at 353.983 kb on - strand at 354.001 kb on - strand at 354.004 kb on + strand at 354.004 kb on + strand at 354.046 kb on - strand at 354.047 kb on + strand at 354.066 kb on - strand at 354.066 kb on - strand at 354.083 kb on - strand at 354.098 kb on - strand at 354.133 kb on - strand, within MMJJ_RS01845 at 354.209 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand at 354.229 kb on + strand, within MMJJ_RS01845 at 354.229 kb on + strand, within MMJJ_RS01845 at 354.421 kb on - strand, within MMJJ_RS01845 at 354.422 kb on + strand, within MMJJ_RS01845 at 354.428 kb on - strand, within MMJJ_RS01845 at 354.457 kb on - strand, within MMJJ_RS01845 at 354.477 kb on + strand, within MMJJ_RS01845 at 354.477 kb on + strand, within MMJJ_RS01845 at 354.496 kb on - strand, within MMJJ_RS01845 at 354.496 kb on - strand, within MMJJ_RS01845 at 354.521 kb on - strand, within MMJJ_RS01845 at 354.521 kb on - strand, within MMJJ_RS01845 at 354.522 kb on + strand, within MMJJ_RS01845 at 354.559 kb on - strand, within MMJJ_RS01845 at 354.559 kb on - strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.564 kb on - strand, within MMJJ_RS01845 at 354.564 kb on - strand, within MMJJ_RS01845 at 354.565 kb on + strand, within MMJJ_RS01845 at 354.591 kb on - strand, within MMJJ_RS01845 at 354.591 kb on - strand, within MMJJ_RS01845 at 354.591 kb on - strand, within MMJJ_RS01845 at 354.592 kb on + strand, within MMJJ_RS01845 at 354.640 kb on + strand, within MMJJ_RS01845 at 354.679 kb on + strand, within MMJJ_RS01845 at 354.691 kb on - strand, within MMJJ_RS01845 at 354.791 kb on - strand at 354.791 kb on - strand at 354.791 kb on - strand at 354.804 kb on - strand at 354.813 kb on - strand at 354.813 kb on - strand at 354.816 kb on + strand at 354.819 kb on - strand at 354.840 kb on + strand at 354.846 kb on + strand at 354.877 kb on + strand at 354.877 kb on + strand at 354.877 kb on + strand at 354.878 kb on - strand at 354.878 kb on - strand at 354.878 kb on - strand at 354.879 kb on + strand at 354.879 kb on + strand at 354.882 kb on - strand at 354.883 kb on + strand at 354.883 kb on + strand at 354.892 kb on - strand at 354.896 kb on - strand at 354.897 kb on + strand at 354.900 kb on + strand at 354.915 kb on + strand at 354.920 kb on - strand at 354.921 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Novobiocin 0.0125 mM remove 352,432 - MMJJ_RS01835 0.44 -2.1 352,472 + MMJJ_RS01835 0.47 -1.7 352,482 - MMJJ_RS01835 0.48 +0.8 352,482 - MMJJ_RS01835 0.48 +1.5 352,482 - MMJJ_RS01835 0.48 +1.4 352,482 - MMJJ_RS01835 0.48 +2.3 352,483 + MMJJ_RS01835 0.48 +1.6 352,486 - MMJJ_RS01835 0.48 +0.5 352,486 - MMJJ_RS01835 0.48 -0.4 352,487 + MMJJ_RS01835 0.48 -1.0 352,489 - MMJJ_RS01835 0.48 -0.9 352,489 - MMJJ_RS01835 0.48 -1.5 352,490 + MMJJ_RS01835 0.48 +0.7 352,495 + MMJJ_RS01835 0.49 -3.1 352,539 + MMJJ_RS01835 0.52 -2.2 352,558 + MMJJ_RS01835 0.53 -3.6 352,558 + MMJJ_RS01835 0.53 +0.4 352,558 + MMJJ_RS01835 0.53 -1.4 352,584 - MMJJ_RS01835 0.55 +1.8 352,590 - MMJJ_RS01835 0.56 -0.2 352,590 - MMJJ_RS01835 0.56 +1.9 352,590 - MMJJ_RS01835 0.56 -0.1 352,591 + MMJJ_RS01835 0.56 +0.1 352,591 + MMJJ_RS01835 0.56 -2.1 352,606 - MMJJ_RS01835 0.57 +0.1 352,607 + MMJJ_RS01835 0.57 +1.0 352,686 + MMJJ_RS01835 0.63 +0.9 352,722 - MMJJ_RS01835 0.66 +0.6 352,723 + +1.1 352,742 - MMJJ_RS01835 0.68 -1.5 352,806 - MMJJ_RS01835 0.73 -0.1 352,806 - MMJJ_RS01835 0.73 +0.8 352,806 - MMJJ_RS01835 0.73 -1.8 352,807 + MMJJ_RS01835 0.73 -0.3 352,807 + MMJJ_RS01835 0.73 +0.8 352,807 + MMJJ_RS01835 0.73 -1.1 352,811 + MMJJ_RS01835 0.73 -1.1 352,811 + MMJJ_RS01835 0.73 +2.0 352,858 - MMJJ_RS01835 0.77 +1.1 352,868 - MMJJ_RS01835 0.77 -2.7 352,869 + MMJJ_RS01835 0.77 -3.7 352,869 + MMJJ_RS01835 0.77 -1.1 352,870 - -0.5 352,871 + MMJJ_RS01835 0.78 +0.1 352,913 - MMJJ_RS01835 0.81 +1.6 352,913 - MMJJ_RS01835 0.81 +1.2 352,914 + MMJJ_RS01835 0.81 -0.6 353,038 - -1.6 353,038 - -3.0 353,049 - -2.7 353,050 + +0.5 353,057 - +0.6 353,096 - +2.7 353,166 - +0.5 353,166 - -1.4 353,166 - -2.5 353,166 - +3.0 353,167 + -2.5 353,167 + -1.5 353,167 + -2.5 353,167 + -1.7 353,167 + +1.8 353,168 - +1.0 353,168 - +1.0 353,168 - -1.9 353,169 + -0.4 353,169 + +1.4 353,169 + +2.8 353,169 + +1.9 353,169 + +2.1 353,169 + -0.7 353,169 + -1.4 353,169 + -0.0 353,170 - +1.7 353,170 - -0.8 353,170 - -2.9 353,170 - -2.3 353,170 - -0.3 353,170 - +0.7 353,170 - +2.2 353,170 - +1.1 353,170 - -0.5 353,171 + +0.5 353,171 + -1.2 353,171 + -0.1 353,171 + +0.2 353,171 + -0.1 353,171 + +3.9 353,171 + +3.1 353,171 + +1.7 353,171 + +0.3 353,171 + +1.5 353,172 - +1.1 353,172 - +2.2 353,172 - -1.3 353,172 - -0.0 353,173 + +1.3 353,173 + -2.9 353,190 - -1.1 353,190 - +2.6 353,190 - -0.9 353,191 + -1.7 353,235 + +1.4 353,235 + +2.4 353,235 + -0.5 353,293 - -0.9 353,293 - +2.9 353,294 + -1.9 353,294 + -3.6 353,298 + +1.3 353,302 - +0.5 353,430 + -1.3 353,433 - MMJJ_RS01840 0.10 +0.7 353,539 + MMJJ_RS01840 0.29 -4.1 353,742 + MMJJ_RS01840 0.64 -2.4 353,745 - MMJJ_RS01840 0.65 -1.9 353,745 - +0.1 353,748 - MMJJ_RS01840 0.65 +0.5 353,749 + MMJJ_RS01840 0.65 -1.7 353,750 - MMJJ_RS01840 0.65 +2.5 353,751 + MMJJ_RS01840 0.66 -1.2 353,771 - MMJJ_RS01840 0.69 +1.1 353,772 + MMJJ_RS01840 0.69 -1.7 353,778 - MMJJ_RS01840 0.70 -2.5 353,797 - MMJJ_RS01840 0.74 -0.5 353,797 - MMJJ_RS01840 0.74 +0.7 353,797 - MMJJ_RS01840 0.74 +2.7 353,798 + MMJJ_RS01840 0.74 -2.9 353,807 - MMJJ_RS01840 0.75 -1.1 353,807 - MMJJ_RS01840 0.75 +2.1 353,807 - MMJJ_RS01840 0.75 -0.4 353,808 + MMJJ_RS01840 0.76 -0.7 353,838 - MMJJ_RS01840 0.81 +2.3 353,838 - MMJJ_RS01840 0.81 +0.5 353,838 - MMJJ_RS01840 0.81 +2.6 353,838 - MMJJ_RS01840 0.81 -3.1 353,838 - MMJJ_RS01840 0.81 -3.8 353,839 + MMJJ_RS01840 0.81 -1.7 353,839 + MMJJ_RS01840 0.81 -2.3 353,839 + MMJJ_RS01840 0.81 -3.7 353,839 + MMJJ_RS01840 0.81 -3.5 353,839 + MMJJ_RS01840 0.81 +2.4 353,839 + MMJJ_RS01840 0.81 -0.3 353,850 + MMJJ_RS01840 0.83 -0.3 353,852 - MMJJ_RS01840 0.83 +1.5 353,853 + MMJJ_RS01840 0.83 +1.5 353,886 + MMJJ_RS01840 0.89 -2.1 353,930 - -3.1 353,966 - -1.4 353,967 + +0.4 353,983 - +1.9 354,001 - -0.4 354,004 + +1.0 354,004 + -3.4 354,046 - -0.9 354,047 + +0.4 354,066 - -0.8 354,066 - +1.1 354,083 - +2.0 354,098 - +1.3 354,133 - MMJJ_RS01845 0.11 -1.7 354,209 - MMJJ_RS01845 0.20 -1.9 354,228 - MMJJ_RS01845 0.23 -2.8 354,228 - MMJJ_RS01845 0.23 +1.1 354,228 - MMJJ_RS01845 0.23 +1.8 354,228 - +0.1 354,229 + MMJJ_RS01845 0.23 +0.7 354,229 + MMJJ_RS01845 0.23 -1.9 354,421 - MMJJ_RS01845 0.47 +1.1 354,422 + MMJJ_RS01845 0.47 -4.6 354,428 - MMJJ_RS01845 0.48 +2.7 354,457 - MMJJ_RS01845 0.51 -0.5 354,477 + MMJJ_RS01845 0.54 +1.0 354,477 + MMJJ_RS01845 0.54 +1.8 354,496 - MMJJ_RS01845 0.56 -0.5 354,496 - MMJJ_RS01845 0.56 -1.3 354,521 - MMJJ_RS01845 0.59 -0.6 354,521 - MMJJ_RS01845 0.59 +1.7 354,522 + MMJJ_RS01845 0.60 +1.7 354,559 - MMJJ_RS01845 0.64 +0.7 354,559 - MMJJ_RS01845 0.64 +2.3 354,560 + MMJJ_RS01845 0.64 -1.5 354,560 + MMJJ_RS01845 0.64 -1.6 354,560 + MMJJ_RS01845 0.64 +3.0 354,560 + MMJJ_RS01845 0.64 +2.3 354,564 - MMJJ_RS01845 0.65 -1.0 354,564 - MMJJ_RS01845 0.65 +0.1 354,565 + MMJJ_RS01845 0.65 -3.5 354,591 - MMJJ_RS01845 0.68 -0.3 354,591 - MMJJ_RS01845 0.68 +1.3 354,591 - MMJJ_RS01845 0.68 +0.0 354,592 + MMJJ_RS01845 0.68 +2.7 354,640 + MMJJ_RS01845 0.74 -1.6 354,679 + MMJJ_RS01845 0.79 -2.4 354,691 - MMJJ_RS01845 0.81 -0.5 354,791 - +1.5 354,791 - +0.2 354,791 - -3.1 354,804 - +2.8 354,813 - -1.9 354,813 - +1.4 354,816 + -3.2 354,819 - +0.1 354,840 + -4.3 354,846 + -2.3 354,877 + +1.3 354,877 + -4.0 354,877 + -3.1 354,878 - +2.6 354,878 - -0.6 354,878 - +0.4 354,879 + +0.6 354,879 + +0.0 354,882 - -1.5 354,883 + +0.4 354,883 + +0.3 354,892 - +1.5 354,896 - +1.2 354,897 + -3.3 354,900 + -0.0 354,915 + +3.1 354,920 - +1.0 354,921 + +0.2
Or see this region's nucleotide sequence