Experiment: McCas_formate_complete Novobiocin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00870 and MMJJ_RS00875 are separated by 16 nucleotides MMJJ_RS00875 and MMJJ_RS00880 are separated by 358 nucleotides MMJJ_RS00880 and MMJJ_RS00885 are separated by 13 nucleotides MMJJ_RS00885 and MMJJ_RS00890 are separated by 10 nucleotides
MMJJ_RS00870: MMJJ_RS00870 - F420-nonreducing hydrogenase, at 167,113 to 168,015
_RS00870
MMJJ_RS00875: MMJJ_RS00875 - hydrogenase iron-sulfur subunit, at 168,032 to 168,454
_RS00875
MMJJ_RS00880: MMJJ_RS00880 - coenzyme F420 hydrogenase subunit alpha, at 168,813 to 170,045
_RS00880
MMJJ_RS00885: MMJJ_RS00885 - coenzyme F420-reducing hydrogenase, FrhD protein, at 170,059 to 170,559
_RS00885
MMJJ_RS00890: MMJJ_RS00890 - coenzyme F420 hydrogenase subunit gamma, at 170,570 to 171,298
_RS00890
Position (kb) 168
169
170
171 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 167.866 kb on + strand, within MMJJ_RS00870 at 167.950 kb on - strand at 167.950 kb on - strand at 167.965 kb on - strand at 168.001 kb on + strand at 168.001 kb on + strand at 168.001 kb on + strand at 168.001 kb on + strand at 168.010 kb on - strand at 168.015 kb on - strand at 168.015 kb on - strand at 168.029 kb on - strand at 168.029 kb on - strand at 168.030 kb on + strand at 168.232 kb on + strand, within MMJJ_RS00875 at 168.262 kb on - strand, within MMJJ_RS00875 at 168.301 kb on - strand, within MMJJ_RS00875 at 168.302 kb on + strand, within MMJJ_RS00875 at 168.302 kb on + strand, within MMJJ_RS00875 at 168.339 kb on + strand, within MMJJ_RS00875 at 168.339 kb on + strand, within MMJJ_RS00875 at 168.341 kb on - strand, within MMJJ_RS00875 at 168.342 kb on + strand, within MMJJ_RS00875 at 168.342 kb on + strand, within MMJJ_RS00875 at 168.348 kb on + strand, within MMJJ_RS00875 at 168.371 kb on + strand, within MMJJ_RS00875 at 168.386 kb on + strand, within MMJJ_RS00875 at 168.399 kb on - strand, within MMJJ_RS00875 at 168.399 kb on - strand, within MMJJ_RS00875 at 168.399 kb on - strand, within MMJJ_RS00875 at 168.400 kb on + strand, within MMJJ_RS00875 at 168.400 kb on + strand, within MMJJ_RS00875 at 168.400 kb on + strand, within MMJJ_RS00875 at 168.400 kb on + strand, within MMJJ_RS00875 at 168.403 kb on - strand, within MMJJ_RS00875 at 168.404 kb on + strand, within MMJJ_RS00875 at 168.705 kb on - strand at 168.706 kb on + strand at 168.706 kb on + strand at 168.708 kb on + strand at 168.803 kb on + strand at 168.832 kb on - strand at 168.840 kb on + strand at 168.908 kb on - strand at 168.909 kb on + strand at 168.912 kb on - strand at 168.912 kb on - strand at 168.912 kb on - strand at 168.913 kb on + strand at 168.932 kb on - strand at 169.026 kb on + strand, within MMJJ_RS00880 at 169.036 kb on - strand, within MMJJ_RS00880 at 169.065 kb on + strand, within MMJJ_RS00880 at 169.189 kb on + strand, within MMJJ_RS00880 at 169.189 kb on + strand, within MMJJ_RS00880 at 169.193 kb on + strand, within MMJJ_RS00880 at 169.227 kb on - strand, within MMJJ_RS00880 at 169.238 kb on - strand, within MMJJ_RS00880 at 169.239 kb on + strand, within MMJJ_RS00880 at 169.243 kb on - strand, within MMJJ_RS00880 at 169.243 kb on - strand, within MMJJ_RS00880 at 169.243 kb on - strand, within MMJJ_RS00880 at 169.244 kb on + strand, within MMJJ_RS00880 at 169.244 kb on + strand, within MMJJ_RS00880 at 169.244 kb on + strand, within MMJJ_RS00880 at 169.287 kb on + strand, within MMJJ_RS00880 at 169.325 kb on + strand, within MMJJ_RS00880 at 169.329 kb on - strand, within MMJJ_RS00880 at 169.345 kb on + strand, within MMJJ_RS00880 at 169.397 kb on - strand, within MMJJ_RS00880 at 169.398 kb on - strand, within MMJJ_RS00880 at 169.400 kb on - strand, within MMJJ_RS00880 at 169.401 kb on + strand, within MMJJ_RS00880 at 169.401 kb on + strand, within MMJJ_RS00880 at 169.435 kb on - strand, within MMJJ_RS00880 at 169.456 kb on + strand, within MMJJ_RS00880 at 169.519 kb on + strand, within MMJJ_RS00880 at 169.520 kb on - strand, within MMJJ_RS00880 at 169.521 kb on + strand, within MMJJ_RS00880 at 169.521 kb on + strand, within MMJJ_RS00880 at 169.527 kb on - strand, within MMJJ_RS00880 at 169.527 kb on - strand, within MMJJ_RS00880 at 169.527 kb on - strand, within MMJJ_RS00880 at 169.536 kb on - strand, within MMJJ_RS00880 at 169.565 kb on - strand, within MMJJ_RS00880 at 169.565 kb on - strand, within MMJJ_RS00880 at 169.565 kb on - strand, within MMJJ_RS00880 at 169.566 kb on + strand, within MMJJ_RS00880 at 169.566 kb on + strand, within MMJJ_RS00880 at 169.569 kb on + strand, within MMJJ_RS00880 at 169.569 kb on + strand, within MMJJ_RS00880 at 169.569 kb on + strand, within MMJJ_RS00880 at 169.581 kb on - strand, within MMJJ_RS00880 at 169.581 kb on - strand, within MMJJ_RS00880 at 169.610 kb on - strand, within MMJJ_RS00880 at 169.611 kb on + strand, within MMJJ_RS00880 at 169.611 kb on + strand, within MMJJ_RS00880 at 169.640 kb on - strand, within MMJJ_RS00880 at 169.664 kb on + strand, within MMJJ_RS00880 at 169.664 kb on + strand, within MMJJ_RS00880 at 169.691 kb on + strand, within MMJJ_RS00880 at 169.691 kb on + strand, within MMJJ_RS00880 at 169.694 kb on + strand, within MMJJ_RS00880 at 169.729 kb on - strand, within MMJJ_RS00880 at 169.859 kb on - strand, within MMJJ_RS00880 at 169.868 kb on - strand, within MMJJ_RS00880 at 169.868 kb on - strand, within MMJJ_RS00880 at 169.916 kb on - strand, within MMJJ_RS00880 at 169.917 kb on + strand, within MMJJ_RS00880 at 169.917 kb on + strand, within MMJJ_RS00880 at 169.955 kb on + strand at 169.955 kb on + strand at 169.955 kb on + strand at 169.963 kb on + strand at 169.994 kb on + strand at 170.007 kb on - strand at 170.039 kb on + strand at 170.044 kb on + strand at 170.072 kb on - strand at 170.142 kb on + strand, within MMJJ_RS00885 at 170.148 kb on - strand, within MMJJ_RS00885 at 170.148 kb on - strand, within MMJJ_RS00885 at 170.149 kb on + strand, within MMJJ_RS00885 at 170.180 kb on - strand, within MMJJ_RS00885 at 170.180 kb on - strand at 170.181 kb on + strand, within MMJJ_RS00885 at 170.236 kb on + strand, within MMJJ_RS00885 at 170.237 kb on - strand, within MMJJ_RS00885 at 170.403 kb on + strand, within MMJJ_RS00885 at 170.418 kb on - strand, within MMJJ_RS00885 at 170.422 kb on - strand, within MMJJ_RS00885 at 170.566 kb on - strand at 170.587 kb on - strand at 170.590 kb on + strand at 170.654 kb on + strand, within MMJJ_RS00890 at 170.673 kb on - strand, within MMJJ_RS00890 at 170.768 kb on + strand, within MMJJ_RS00890 at 170.796 kb on - strand, within MMJJ_RS00890 at 170.826 kb on - strand, within MMJJ_RS00890 at 170.827 kb on + strand, within MMJJ_RS00890 at 170.857 kb on + strand, within MMJJ_RS00890 at 170.890 kb on - strand, within MMJJ_RS00890 at 170.893 kb on - strand, within MMJJ_RS00890 at 170.893 kb on - strand, within MMJJ_RS00890 at 170.958 kb on - strand, within MMJJ_RS00890 at 170.958 kb on - strand, within MMJJ_RS00890 at 170.959 kb on + strand, within MMJJ_RS00890 at 170.959 kb on + strand, within MMJJ_RS00890 at 170.968 kb on + strand, within MMJJ_RS00890 at 171.012 kb on - strand, within MMJJ_RS00890 at 171.022 kb on - strand, within MMJJ_RS00890 at 171.022 kb on - strand, within MMJJ_RS00890 at 171.022 kb on - strand, within MMJJ_RS00890 at 171.023 kb on + strand at 171.023 kb on + strand, within MMJJ_RS00890
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Novobiocin 0.0125 mM remove 167,866 + MMJJ_RS00870 0.83 +2.9 167,950 - -1.5 167,950 - -4.0 167,965 - +2.5 168,001 + -1.0 168,001 + +4.1 168,001 + -1.0 168,001 + -2.6 168,010 - -2.3 168,015 - -0.6 168,015 - -0.6 168,029 - +0.4 168,029 - -1.7 168,030 + +1.8 168,232 + MMJJ_RS00875 0.47 +3.0 168,262 - MMJJ_RS00875 0.54 -0.2 168,301 - MMJJ_RS00875 0.64 -0.3 168,302 + MMJJ_RS00875 0.64 -1.3 168,302 + MMJJ_RS00875 0.64 +0.1 168,339 + MMJJ_RS00875 0.73 -1.0 168,339 + MMJJ_RS00875 0.73 +2.1 168,341 - MMJJ_RS00875 0.73 -1.5 168,342 + MMJJ_RS00875 0.73 -4.1 168,342 + MMJJ_RS00875 0.73 +1.2 168,348 + MMJJ_RS00875 0.75 -0.4 168,371 + MMJJ_RS00875 0.80 +2.1 168,386 + MMJJ_RS00875 0.84 +1.6 168,399 - MMJJ_RS00875 0.87 -0.5 168,399 - MMJJ_RS00875 0.87 -2.8 168,399 - MMJJ_RS00875 0.87 -0.3 168,400 + MMJJ_RS00875 0.87 +0.2 168,400 + MMJJ_RS00875 0.87 +0.2 168,400 + MMJJ_RS00875 0.87 +0.9 168,400 + MMJJ_RS00875 0.87 -1.2 168,403 - MMJJ_RS00875 0.88 -2.6 168,404 + MMJJ_RS00875 0.88 +1.5 168,705 - -3.3 168,706 + -2.0 168,706 + +1.5 168,708 + -1.7 168,803 + -2.9 168,832 - +0.5 168,840 + +0.7 168,908 - +0.2 168,909 + -4.1 168,912 - +1.7 168,912 - +2.1 168,912 - -1.8 168,913 + -2.9 168,932 - -1.8 169,026 + MMJJ_RS00880 0.17 -0.2 169,036 - MMJJ_RS00880 0.18 +1.8 169,065 + MMJJ_RS00880 0.20 +1.4 169,189 + MMJJ_RS00880 0.30 -1.7 169,189 + MMJJ_RS00880 0.30 +3.3 169,193 + MMJJ_RS00880 0.31 -3.4 169,227 - MMJJ_RS00880 0.34 -0.1 169,238 - MMJJ_RS00880 0.34 +1.5 169,239 + MMJJ_RS00880 0.35 -2.5 169,243 - MMJJ_RS00880 0.35 +2.3 169,243 - MMJJ_RS00880 0.35 +0.8 169,243 - MMJJ_RS00880 0.35 -1.6 169,244 + MMJJ_RS00880 0.35 -2.0 169,244 + MMJJ_RS00880 0.35 -0.5 169,244 + MMJJ_RS00880 0.35 -1.9 169,287 + MMJJ_RS00880 0.38 -2.6 169,325 + MMJJ_RS00880 0.42 +1.9 169,329 - MMJJ_RS00880 0.42 +3.8 169,345 + MMJJ_RS00880 0.43 -1.0 169,397 - MMJJ_RS00880 0.47 -1.6 169,398 - MMJJ_RS00880 0.47 -2.6 169,400 - MMJJ_RS00880 0.48 +2.5 169,401 + MMJJ_RS00880 0.48 +0.8 169,401 + MMJJ_RS00880 0.48 +2.8 169,435 - MMJJ_RS00880 0.50 +0.5 169,456 + MMJJ_RS00880 0.52 -3.8 169,519 + MMJJ_RS00880 0.57 -0.4 169,520 - MMJJ_RS00880 0.57 -0.7 169,521 + MMJJ_RS00880 0.57 +2.8 169,521 + MMJJ_RS00880 0.57 -2.2 169,527 - MMJJ_RS00880 0.58 +2.0 169,527 - MMJJ_RS00880 0.58 -1.2 169,527 - MMJJ_RS00880 0.58 -2.3 169,536 - MMJJ_RS00880 0.59 +2.9 169,565 - MMJJ_RS00880 0.61 -0.5 169,565 - MMJJ_RS00880 0.61 +1.8 169,565 - MMJJ_RS00880 0.61 +3.3 169,566 + MMJJ_RS00880 0.61 -1.8 169,566 + MMJJ_RS00880 0.61 +0.8 169,569 + MMJJ_RS00880 0.61 +2.5 169,569 + MMJJ_RS00880 0.61 +0.9 169,569 + MMJJ_RS00880 0.61 +3.8 169,581 - MMJJ_RS00880 0.62 +2.0 169,581 - MMJJ_RS00880 0.62 +0.7 169,610 - MMJJ_RS00880 0.65 +1.4 169,611 + MMJJ_RS00880 0.65 -0.2 169,611 + MMJJ_RS00880 0.65 -0.1 169,640 - MMJJ_RS00880 0.67 +1.7 169,664 + MMJJ_RS00880 0.69 -2.2 169,664 + MMJJ_RS00880 0.69 +2.1 169,691 + MMJJ_RS00880 0.71 -0.7 169,691 + MMJJ_RS00880 0.71 -1.3 169,694 + MMJJ_RS00880 0.71 +0.4 169,729 - MMJJ_RS00880 0.74 -0.1 169,859 - MMJJ_RS00880 0.85 -0.6 169,868 - MMJJ_RS00880 0.86 +1.2 169,868 - MMJJ_RS00880 0.86 -1.6 169,916 - MMJJ_RS00880 0.89 +4.1 169,917 + MMJJ_RS00880 0.90 -1.0 169,917 + MMJJ_RS00880 0.90 -1.3 169,955 + +2.1 169,955 + -0.9 169,955 + -3.0 169,963 + -2.7 169,994 + -0.6 170,007 - -2.6 170,039 + -1.0 170,044 + -1.2 170,072 - -2.6 170,142 + MMJJ_RS00885 0.17 -1.1 170,148 - MMJJ_RS00885 0.18 -1.1 170,148 - MMJJ_RS00885 0.18 -2.7 170,149 + MMJJ_RS00885 0.18 +1.5 170,180 - MMJJ_RS00885 0.24 -2.3 170,180 - +1.0 170,181 + MMJJ_RS00885 0.24 -3.5 170,236 + MMJJ_RS00885 0.35 -1.0 170,237 - MMJJ_RS00885 0.36 -0.7 170,403 + MMJJ_RS00885 0.69 -1.3 170,418 - MMJJ_RS00885 0.72 -0.8 170,422 - MMJJ_RS00885 0.72 +2.8 170,566 - -3.4 170,587 - -1.5 170,590 + -1.3 170,654 + MMJJ_RS00890 0.12 -2.0 170,673 - MMJJ_RS00890 0.14 -0.8 170,768 + MMJJ_RS00890 0.27 +1.7 170,796 - MMJJ_RS00890 0.31 +0.3 170,826 - MMJJ_RS00890 0.35 +3.0 170,827 + MMJJ_RS00890 0.35 +1.0 170,857 + MMJJ_RS00890 0.39 -2.8 170,890 - MMJJ_RS00890 0.44 -0.6 170,893 - MMJJ_RS00890 0.44 -1.8 170,893 - MMJJ_RS00890 0.44 -0.2 170,958 - MMJJ_RS00890 0.53 -2.6 170,958 - MMJJ_RS00890 0.53 +3.0 170,959 + MMJJ_RS00890 0.53 +1.5 170,959 + MMJJ_RS00890 0.53 +2.1 170,968 + MMJJ_RS00890 0.55 -1.4 171,012 - MMJJ_RS00890 0.61 +2.2 171,022 - MMJJ_RS00890 0.62 +1.0 171,022 - MMJJ_RS00890 0.62 +0.9 171,022 - MMJJ_RS00890 0.62 -0.7 171,023 + -0.0 171,023 + MMJJ_RS00890 0.62 +1.9
Or see this region's nucleotide sequence