Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00485

Experiment: McCas_formate_complete Novobiocin 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00480 and MMJJ_RS00485 are separated by 21 nucleotidesMMJJ_RS00485 and MMJJ_RS00490 are separated by 124 nucleotides MMJJ_RS00480: MMJJ_RS00480 - flippase, at 88,712 to 89,983 _RS00480 MMJJ_RS00485: MMJJ_RS00485 - tetratricopeptide repeat protein, at 90,005 to 90,598 _RS00485 MMJJ_RS00490: MMJJ_RS00490 - glutamine-hydrolyzing GMP synthase, at 90,723 to 91,655 _RS00490 Position (kb) 90 91Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 89.018 kb on - strand, within MMJJ_RS00480at 89.018 kb on - strand, within MMJJ_RS00480at 89.060 kb on - strand, within MMJJ_RS00480at 89.141 kb on - strand, within MMJJ_RS00480at 89.142 kb on + strand, within MMJJ_RS00480at 89.214 kb on - strand, within MMJJ_RS00480at 89.234 kb on - strand, within MMJJ_RS00480at 89.235 kb on + strand, within MMJJ_RS00480at 89.281 kb on - strand, within MMJJ_RS00480at 89.304 kb on - strand, within MMJJ_RS00480at 89.304 kb on - strandat 89.304 kb on - strand, within MMJJ_RS00480at 89.305 kb on + strand, within MMJJ_RS00480at 89.305 kb on + strand, within MMJJ_RS00480at 89.305 kb on + strand, within MMJJ_RS00480at 89.305 kb on + strand, within MMJJ_RS00480at 89.307 kb on + strand, within MMJJ_RS00480at 89.307 kb on + strand, within MMJJ_RS00480at 89.403 kb on - strand, within MMJJ_RS00480at 89.475 kb on - strand, within MMJJ_RS00480at 89.475 kb on - strand, within MMJJ_RS00480at 89.564 kb on - strand, within MMJJ_RS00480at 89.607 kb on + strand, within MMJJ_RS00480at 89.646 kb on - strand, within MMJJ_RS00480at 89.676 kb on + strand, within MMJJ_RS00480at 89.715 kb on + strand, within MMJJ_RS00480at 89.726 kb on + strand, within MMJJ_RS00480at 89.730 kb on - strand, within MMJJ_RS00480at 89.731 kb on + strand, within MMJJ_RS00480at 89.809 kb on - strand, within MMJJ_RS00480at 89.813 kb on - strand, within MMJJ_RS00480at 89.818 kb on - strand, within MMJJ_RS00480at 89.880 kb on + strandat 89.899 kb on + strandat 89.917 kb on + strandat 89.917 kb on + strandat 89.917 kb on + strandat 89.937 kb on - strandat 89.938 kb on + strandat 89.938 kb on + strandat 89.938 kb on + strandat 89.978 kb on + strandat 90.069 kb on + strand, within MMJJ_RS00485at 90.123 kb on - strand, within MMJJ_RS00485at 90.143 kb on + strand, within MMJJ_RS00485at 90.175 kb on - strand, within MMJJ_RS00485at 90.175 kb on - strand, within MMJJ_RS00485at 90.176 kb on + strand, within MMJJ_RS00485at 90.225 kb on + strand, within MMJJ_RS00485at 90.280 kb on + strand, within MMJJ_RS00485at 90.377 kb on + strand, within MMJJ_RS00485at 90.377 kb on + strand, within MMJJ_RS00485at 90.474 kb on + strand, within MMJJ_RS00485at 90.474 kb on + strand, within MMJJ_RS00485at 90.501 kb on + strand, within MMJJ_RS00485at 90.505 kb on - strand, within MMJJ_RS00485at 90.510 kb on - strand, within MMJJ_RS00485at 90.531 kb on - strand, within MMJJ_RS00485at 90.532 kb on + strand, within MMJJ_RS00485at 90.644 kb on + strandat 90.666 kb on - strandat 90.666 kb on - strandat 90.670 kb on - strandat 90.670 kb on - strandat 90.671 kb on + strandat 90.671 kb on + strandat 90.674 kb on - strandat 90.674 kb on - strandat 90.675 kb on + strandat 90.681 kb on - strandat 90.711 kb on - strandat 90.712 kb on + strandat 90.712 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Novobiocin 0.0125 mM
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89,018 - MMJJ_RS00480 0.24 +1.1
89,018 - MMJJ_RS00480 0.24 -1.0
89,060 - MMJJ_RS00480 0.27 -0.4
89,141 - MMJJ_RS00480 0.34 +1.4
89,142 + MMJJ_RS00480 0.34 -3.1
89,214 - MMJJ_RS00480 0.39 -0.0
89,234 - MMJJ_RS00480 0.41 +1.4
89,235 + MMJJ_RS00480 0.41 +0.0
89,281 - MMJJ_RS00480 0.45 +1.0
89,304 - MMJJ_RS00480 0.47 -1.5
89,304 - +1.0
89,304 - MMJJ_RS00480 0.47 -0.5
89,305 + MMJJ_RS00480 0.47 +0.6
89,305 + MMJJ_RS00480 0.47 -3.3
89,305 + MMJJ_RS00480 0.47 -4.2
89,305 + MMJJ_RS00480 0.47 +1.2
89,307 + MMJJ_RS00480 0.47 -4.0
89,307 + MMJJ_RS00480 0.47 -2.2
89,403 - MMJJ_RS00480 0.54 +0.3
89,475 - MMJJ_RS00480 0.60 -2.6
89,475 - MMJJ_RS00480 0.60 -3.0
89,564 - MMJJ_RS00480 0.67 -0.5
89,607 + MMJJ_RS00480 0.70 -0.1
89,646 - MMJJ_RS00480 0.73 -3.0
89,676 + MMJJ_RS00480 0.76 -0.7
89,715 + MMJJ_RS00480 0.79 -0.2
89,726 + MMJJ_RS00480 0.80 -1.3
89,730 - MMJJ_RS00480 0.80 +0.1
89,731 + MMJJ_RS00480 0.80 -0.4
89,809 - MMJJ_RS00480 0.86 -3.2
89,813 - MMJJ_RS00480 0.87 -0.3
89,818 - MMJJ_RS00480 0.87 +0.8
89,880 + -1.0
89,899 + -1.2
89,917 + -2.9
89,917 + -0.1
89,917 + +1.2
89,937 - +1.0
89,938 + -2.3
89,938 + +1.7
89,938 + -0.3
89,978 + -1.1
90,069 + MMJJ_RS00485 0.11 -0.3
90,123 - MMJJ_RS00485 0.20 +3.2
90,143 + MMJJ_RS00485 0.23 -4.0
90,175 - MMJJ_RS00485 0.29 +2.7
90,175 - MMJJ_RS00485 0.29 +2.7
90,176 + MMJJ_RS00485 0.29 -2.5
90,225 + MMJJ_RS00485 0.37 +0.5
90,280 + MMJJ_RS00485 0.46 +0.1
90,377 + MMJJ_RS00485 0.63 +2.5
90,377 + MMJJ_RS00485 0.63 +2.2
90,474 + MMJJ_RS00485 0.79 -2.2
90,474 + MMJJ_RS00485 0.79 +2.4
90,501 + MMJJ_RS00485 0.84 -2.9
90,505 - MMJJ_RS00485 0.84 +1.7
90,510 - MMJJ_RS00485 0.85 -0.8
90,531 - MMJJ_RS00485 0.89 -1.5
90,532 + MMJJ_RS00485 0.89 -2.0
90,644 + +2.8
90,666 - -0.2
90,666 - -2.8
90,670 - -1.0
90,670 - +2.2
90,671 + +0.6
90,671 + +0.8
90,674 - -2.2
90,674 - -3.9
90,675 + +2.4
90,681 - +0.9
90,711 - +0.4
90,712 + -0.9
90,712 + +1.2

Or see this region's nucleotide sequence