Experiment: McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08115 and MMJJ_RS08120 are separated by 62 nucleotides MMJJ_RS08120 and MMJJ_RS08125 are separated by 135 nucleotides MMJJ_RS08125 and MMJJ_RS08130 are separated by 36 nucleotides
MMJJ_RS08115: MMJJ_RS08115 - hydroxymethylglutaryl-CoA synthase, at 1,488,909 to 1,489,958
_RS08115
MMJJ_RS08120: MMJJ_RS08120 - helix-turn-helix domain-containing protein, at 1,490,021 to 1,490,710
_RS08120
MMJJ_RS08125: MMJJ_RS08125 - DNA double-strand break repair nuclease NurA, at 1,490,846 to 1,491,910
_RS08125
MMJJ_RS08130: MMJJ_RS08130 - translation initiation factor IF-2 subunit gamma, at 1,491,947 to 1,493,179
_RS08130
Position (kb)
1490
1491
1492 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1489.939 kb on + strand at 1489.948 kb on + strand at 1490.510 kb on - strand at 1490.689 kb on - strand at 1490.813 kb on - strand at 1490.842 kb on - strand at 1490.961 kb on - strand, within MMJJ_RS08125 at 1490.962 kb on + strand, within MMJJ_RS08125 at 1490.962 kb on + strand, within MMJJ_RS08125 at 1490.962 kb on + strand, within MMJJ_RS08125 at 1490.982 kb on + strand, within MMJJ_RS08125 at 1491.114 kb on - strand, within MMJJ_RS08125 at 1491.115 kb on + strand, within MMJJ_RS08125 at 1491.238 kb on + strand, within MMJJ_RS08125 at 1491.241 kb on - strand, within MMJJ_RS08125 at 1491.305 kb on - strand, within MMJJ_RS08125 at 1491.306 kb on + strand, within MMJJ_RS08125 at 1491.309 kb on + strand, within MMJJ_RS08125 at 1491.309 kb on + strand, within MMJJ_RS08125 at 1491.311 kb on - strand, within MMJJ_RS08125 at 1491.311 kb on - strand, within MMJJ_RS08125 at 1491.453 kb on - strand, within MMJJ_RS08125 at 1491.454 kb on + strand, within MMJJ_RS08125 at 1491.454 kb on + strand, within MMJJ_RS08125 at 1491.464 kb on + strand, within MMJJ_RS08125 at 1491.467 kb on + strand, within MMJJ_RS08125 at 1491.488 kb on + strand, within MMJJ_RS08125 at 1491.525 kb on - strand, within MMJJ_RS08125 at 1491.525 kb on - strand, within MMJJ_RS08125 at 1491.526 kb on + strand, within MMJJ_RS08125 at 1491.526 kb on + strand, within MMJJ_RS08125 at 1491.527 kb on - strand, within MMJJ_RS08125 at 1491.527 kb on - strand, within MMJJ_RS08125 at 1491.527 kb on - strand, within MMJJ_RS08125 at 1491.528 kb on + strand, within MMJJ_RS08125 at 1491.528 kb on + strand, within MMJJ_RS08125 at 1491.546 kb on - strand, within MMJJ_RS08125 at 1491.546 kb on - strand, within MMJJ_RS08125 at 1491.546 kb on - strand, within MMJJ_RS08125 at 1491.547 kb on + strand, within MMJJ_RS08125 at 1491.550 kb on + strand, within MMJJ_RS08125 at 1491.633 kb on - strand, within MMJJ_RS08125 at 1491.684 kb on + strand, within MMJJ_RS08125 at 1491.708 kb on - strand, within MMJJ_RS08125 at 1491.708 kb on - strand, within MMJJ_RS08125 at 1491.738 kb on - strand, within MMJJ_RS08125 at 1491.744 kb on + strand, within MMJJ_RS08125 at 1491.744 kb on + strand, within MMJJ_RS08125 at 1491.746 kb on + strand, within MMJJ_RS08125 at 1491.793 kb on - strand, within MMJJ_RS08125 at 1491.806 kb on + strand at 1491.832 kb on - strand at 1491.833 kb on + strand at 1491.866 kb on + strand at 1491.872 kb on - strand at 1491.872 kb on - strand at 1491.916 kb on + strand at 1491.928 kb on - strand at 1491.953 kb on - strand at 1491.954 kb on + strand at 1492.249 kb on - strand at 1492.545 kb on + strand, within MMJJ_RS08130
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM remove 1,489,939 + -0.2 1,489,948 + -0.2 1,490,510 - +0.4 1,490,689 - +0.4 1,490,813 - +5.9 1,490,842 - +2.1 1,490,961 - MMJJ_RS08125 0.11 -1.8 1,490,962 + MMJJ_RS08125 0.11 -0.6 1,490,962 + MMJJ_RS08125 0.11 -0.5 1,490,962 + MMJJ_RS08125 0.11 +2.7 1,490,982 + MMJJ_RS08125 0.13 -1.9 1,491,114 - MMJJ_RS08125 0.25 +1.4 1,491,115 + MMJJ_RS08125 0.25 +3.7 1,491,238 + MMJJ_RS08125 0.37 -1.7 1,491,241 - MMJJ_RS08125 0.37 +1.2 1,491,305 - MMJJ_RS08125 0.43 -1.4 1,491,306 + MMJJ_RS08125 0.43 +0.4 1,491,309 + MMJJ_RS08125 0.43 -3.0 1,491,309 + MMJJ_RS08125 0.43 +3.7 1,491,311 - MMJJ_RS08125 0.44 +2.2 1,491,311 - MMJJ_RS08125 0.44 +3.5 1,491,453 - MMJJ_RS08125 0.57 +0.2 1,491,454 + MMJJ_RS08125 0.57 +0.5 1,491,454 + MMJJ_RS08125 0.57 +0.7 1,491,464 + MMJJ_RS08125 0.58 -1.8 1,491,467 + MMJJ_RS08125 0.58 +0.5 1,491,488 + MMJJ_RS08125 0.60 +2.2 1,491,525 - MMJJ_RS08125 0.64 +2.5 1,491,525 - MMJJ_RS08125 0.64 -4.0 1,491,526 + MMJJ_RS08125 0.64 +2.8 1,491,526 + MMJJ_RS08125 0.64 +0.7 1,491,527 - MMJJ_RS08125 0.64 -3.4 1,491,527 - MMJJ_RS08125 0.64 +1.8 1,491,527 - MMJJ_RS08125 0.64 +3.8 1,491,528 + MMJJ_RS08125 0.64 +1.1 1,491,528 + MMJJ_RS08125 0.64 +2.4 1,491,546 - MMJJ_RS08125 0.66 -4.0 1,491,546 - MMJJ_RS08125 0.66 +1.2 1,491,546 - MMJJ_RS08125 0.66 +3.2 1,491,547 + MMJJ_RS08125 0.66 +3.3 1,491,550 + MMJJ_RS08125 0.66 +0.1 1,491,633 - MMJJ_RS08125 0.74 +2.8 1,491,684 + MMJJ_RS08125 0.79 +0.9 1,491,708 - MMJJ_RS08125 0.81 +4.9 1,491,708 - MMJJ_RS08125 0.81 +4.0 1,491,738 - MMJJ_RS08125 0.84 +2.1 1,491,744 + MMJJ_RS08125 0.84 +3.9 1,491,744 + MMJJ_RS08125 0.84 +0.0 1,491,746 + MMJJ_RS08125 0.85 -2.6 1,491,793 - MMJJ_RS08125 0.89 -0.3 1,491,806 + +2.0 1,491,832 - +1.4 1,491,833 + -0.4 1,491,866 + +1.9 1,491,872 - -0.1 1,491,872 - +0.5 1,491,916 + +3.0 1,491,928 - +2.7 1,491,953 - +1.8 1,491,954 + +0.0 1,492,249 - -0.2 1,492,545 + MMJJ_RS08130 0.48 -3.2
Or see this region's nucleotide sequence