Experiment: McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01510 and MMJJ_RS01515 are separated by 130 nucleotides MMJJ_RS01515 and MMJJ_RS01520 are separated by 10 nucleotides MMJJ_RS01520 and MMJJ_RS01525 are separated by 14 nucleotides MMJJ_RS01525 and MMJJ_RS01530 are separated by 102 nucleotides MMJJ_RS01530 and MMJJ_RS01535 are separated by 9 nucleotides
MMJJ_RS01510: MMJJ_RS01510 - ATP-binding protein, at 295,702 to 297,099
_RS01510
MMJJ_RS01515: MMJJ_RS01515 - DUF2178 domain-containing protein, at 297,230 to 297,616
_RS01515
MMJJ_RS01520: MMJJ_RS01520 - helix-turn-helix transcriptional regulator, at 297,627 to 297,830
_RS01520
MMJJ_RS01525: MMJJ_RS01525 - ketopantoate reductase family protein, at 297,845 to 298,765
_RS01525
MMJJ_RS01530: MMJJ_RS01530 - YwbE family protein, at 298,868 to 299,062
_RS01530
MMJJ_RS01535: MMJJ_RS01535 - DMT family transporter, at 299,072 to 299,962
_RS01535
Position (kb) 297
298
299 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 296.874 kb on - strand, within MMJJ_RS01510 at 296.916 kb on - strand, within MMJJ_RS01510 at 297.015 kb on + strand at 297.066 kb on - strand at 297.069 kb on + strand at 297.073 kb on - strand at 297.074 kb on + strand at 297.076 kb on + strand at 297.088 kb on - strand at 297.107 kb on + strand at 297.115 kb on - strand at 297.120 kb on - strand at 297.121 kb on + strand at 297.126 kb on + strand at 297.126 kb on + strand at 297.126 kb on + strand at 297.129 kb on + strand at 297.195 kb on + strand at 297.208 kb on - strand at 297.254 kb on - strand at 297.259 kb on - strand at 297.273 kb on - strand, within MMJJ_RS01515 at 297.274 kb on + strand, within MMJJ_RS01515 at 297.321 kb on + strand, within MMJJ_RS01515 at 297.343 kb on + strand, within MMJJ_RS01515 at 297.370 kb on - strand, within MMJJ_RS01515 at 297.371 kb on + strand, within MMJJ_RS01515 at 297.394 kb on + strand, within MMJJ_RS01515 at 297.394 kb on + strand, within MMJJ_RS01515 at 297.504 kb on + strand, within MMJJ_RS01515 at 297.504 kb on + strand, within MMJJ_RS01515 at 297.552 kb on - strand, within MMJJ_RS01515 at 297.567 kb on - strand, within MMJJ_RS01515 at 297.567 kb on - strand, within MMJJ_RS01515 at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.641 kb on - strand at 297.651 kb on + strand, within MMJJ_RS01520 at 297.717 kb on + strand, within MMJJ_RS01520 at 297.757 kb on - strand, within MMJJ_RS01520 at 297.757 kb on - strand, within MMJJ_RS01520 at 297.779 kb on + strand, within MMJJ_RS01520 at 297.802 kb on - strand, within MMJJ_RS01520 at 297.802 kb on - strand, within MMJJ_RS01520 at 297.807 kb on + strand, within MMJJ_RS01520 at 297.842 kb on - strand at 297.864 kb on + strand at 297.901 kb on - strand at 297.906 kb on - strand at 297.965 kb on - strand, within MMJJ_RS01525 at 297.965 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.977 kb on + strand, within MMJJ_RS01525 at 298.059 kb on - strand, within MMJJ_RS01525 at 298.124 kb on - strand, within MMJJ_RS01525 at 298.129 kb on - strand, within MMJJ_RS01525 at 298.130 kb on + strand, within MMJJ_RS01525 at 298.130 kb on + strand, within MMJJ_RS01525 at 298.135 kb on - strand, within MMJJ_RS01525 at 298.141 kb on - strand, within MMJJ_RS01525 at 298.142 kb on + strand, within MMJJ_RS01525 at 298.142 kb on + strand, within MMJJ_RS01525 at 298.186 kb on - strand, within MMJJ_RS01525 at 298.217 kb on + strand, within MMJJ_RS01525 at 298.218 kb on - strand, within MMJJ_RS01525 at 298.250 kb on + strand, within MMJJ_RS01525 at 298.361 kb on - strand, within MMJJ_RS01525 at 298.362 kb on + strand, within MMJJ_RS01525 at 298.408 kb on - strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.430 kb on + strand, within MMJJ_RS01525 at 298.435 kb on - strand, within MMJJ_RS01525 at 298.435 kb on - strand, within MMJJ_RS01525 at 298.436 kb on + strand, within MMJJ_RS01525 at 298.436 kb on + strand, within MMJJ_RS01525 at 298.437 kb on - strand, within MMJJ_RS01525 at 298.443 kb on + strand, within MMJJ_RS01525 at 298.500 kb on + strand, within MMJJ_RS01525 at 298.500 kb on + strand, within MMJJ_RS01525 at 298.501 kb on - strand at 298.501 kb on - strand, within MMJJ_RS01525 at 298.553 kb on - strand, within MMJJ_RS01525 at 298.554 kb on + strand, within MMJJ_RS01525 at 298.555 kb on - strand, within MMJJ_RS01525 at 298.555 kb on - strand, within MMJJ_RS01525 at 298.556 kb on + strand, within MMJJ_RS01525 at 298.558 kb on - strand, within MMJJ_RS01525 at 298.558 kb on - strand, within MMJJ_RS01525 at 298.559 kb on + strand, within MMJJ_RS01525 at 298.561 kb on - strand, within MMJJ_RS01525 at 298.563 kb on - strand, within MMJJ_RS01525 at 298.563 kb on - strand, within MMJJ_RS01525 at 298.656 kb on + strand, within MMJJ_RS01525 at 298.700 kb on - strand at 298.721 kb on - strand at 298.727 kb on - strand at 298.754 kb on - strand at 298.820 kb on - strand at 298.820 kb on - strand at 298.821 kb on + strand at 298.823 kb on - strand at 298.826 kb on - strand at 298.827 kb on + strand at 298.835 kb on - strand at 298.836 kb on + strand at 298.839 kb on - strand at 298.840 kb on + strand at 298.843 kb on - strand at 298.843 kb on - strand at 298.844 kb on + strand at 298.844 kb on + strand at 298.870 kb on + strand at 298.922 kb on - strand, within MMJJ_RS01530 at 298.923 kb on + strand, within MMJJ_RS01530 at 299.012 kb on - strand, within MMJJ_RS01530 at 299.037 kb on + strand, within MMJJ_RS01530 at 299.063 kb on + strand at 299.065 kb on - strand at 299.152 kb on + strand at 299.155 kb on - strand at 299.164 kb on - strand, within MMJJ_RS01535 at 299.178 kb on + strand, within MMJJ_RS01535 at 299.212 kb on - strand, within MMJJ_RS01535 at 299.212 kb on - strand, within MMJJ_RS01535 at 299.213 kb on + strand, within MMJJ_RS01535 at 299.214 kb on - strand, within MMJJ_RS01535 at 299.234 kb on + strand, within MMJJ_RS01535 at 299.260 kb on - strand, within MMJJ_RS01535 at 299.274 kb on - strand, within MMJJ_RS01535 at 299.274 kb on - strand, within MMJJ_RS01535 at 299.279 kb on - strand, within MMJJ_RS01535 at 299.280 kb on + strand, within MMJJ_RS01535 at 299.304 kb on - strand, within MMJJ_RS01535 at 299.304 kb on - strand, within MMJJ_RS01535 at 299.340 kb on + strand, within MMJJ_RS01535 at 299.344 kb on - strand, within MMJJ_RS01535 at 299.344 kb on - strand, within MMJJ_RS01535 at 299.345 kb on + strand, within MMJJ_RS01535 at 299.345 kb on + strand, within MMJJ_RS01535 at 299.376 kb on + strand, within MMJJ_RS01535 at 299.383 kb on - strand, within MMJJ_RS01535 at 299.384 kb on + strand, within MMJJ_RS01535 at 299.392 kb on - strand, within MMJJ_RS01535 at 299.392 kb on - strand, within MMJJ_RS01535 at 299.399 kb on - strand, within MMJJ_RS01535 at 299.417 kb on - strand, within MMJJ_RS01535 at 299.418 kb on + strand, within MMJJ_RS01535 at 299.475 kb on - strand, within MMJJ_RS01535 at 299.476 kb on + strand, within MMJJ_RS01535 at 299.488 kb on - strand, within MMJJ_RS01535 at 299.489 kb on + strand, within MMJJ_RS01535 at 299.510 kb on - strand, within MMJJ_RS01535 at 299.511 kb on + strand, within MMJJ_RS01535 at 299.513 kb on + strand, within MMJJ_RS01535 at 299.513 kb on + strand, within MMJJ_RS01535 at 299.513 kb on + strand, within MMJJ_RS01535 at 299.603 kb on - strand, within MMJJ_RS01535 at 299.655 kb on + strand, within MMJJ_RS01535 at 299.655 kb on + strand, within MMJJ_RS01535 at 299.656 kb on - strand, within MMJJ_RS01535 at 299.656 kb on - strand, within MMJJ_RS01535 at 299.661 kb on + strand, within MMJJ_RS01535 at 299.676 kb on + strand, within MMJJ_RS01535 at 299.687 kb on + strand, within MMJJ_RS01535 at 299.687 kb on + strand, within MMJJ_RS01535 at 299.700 kb on - strand, within MMJJ_RS01535 at 299.701 kb on + strand, within MMJJ_RS01535 at 299.706 kb on + strand, within MMJJ_RS01535 at 299.706 kb on + strand, within MMJJ_RS01535 at 299.723 kb on - strand, within MMJJ_RS01535 at 299.727 kb on - strand, within MMJJ_RS01535 at 299.728 kb on + strand, within MMJJ_RS01535 at 299.731 kb on + strand, within MMJJ_RS01535 at 299.737 kb on - strand, within MMJJ_RS01535 at 299.737 kb on - strand, within MMJJ_RS01535
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM remove 296,874 - MMJJ_RS01510 0.84 +0.1 296,916 - MMJJ_RS01510 0.87 +0.7 297,015 + -2.5 297,066 - -0.7 297,069 + +2.5 297,073 - -0.3 297,074 + +2.4 297,076 + +1.2 297,088 - +2.5 297,107 + +0.8 297,115 - +0.2 297,120 - +1.6 297,121 + +0.6 297,126 + -0.8 297,126 + -1.7 297,126 + +2.8 297,129 + +1.7 297,195 + -4.1 297,208 - -3.8 297,254 - -1.5 297,259 - +1.9 297,273 - MMJJ_RS01515 0.11 +2.8 297,274 + MMJJ_RS01515 0.11 +4.0 297,321 + MMJJ_RS01515 0.24 -3.5 297,343 + MMJJ_RS01515 0.29 -2.6 297,370 - MMJJ_RS01515 0.36 -3.0 297,371 + MMJJ_RS01515 0.36 -2.3 297,394 + MMJJ_RS01515 0.42 -2.6 297,394 + MMJJ_RS01515 0.42 -2.5 297,504 + MMJJ_RS01515 0.71 -0.6 297,504 + MMJJ_RS01515 0.71 +1.0 297,552 - MMJJ_RS01515 0.83 +1.6 297,567 - MMJJ_RS01515 0.87 +0.3 297,567 - MMJJ_RS01515 0.87 +2.4 297,588 - +0.8 297,588 - +1.4 297,588 - -3.2 297,588 - -0.9 297,588 - -0.7 297,589 + -0.3 297,589 + -0.2 297,589 + +2.0 297,589 + +1.8 297,589 + -0.2 297,589 + -0.8 297,589 + -0.7 297,641 - -0.3 297,651 + MMJJ_RS01520 0.12 -0.3 297,717 + MMJJ_RS01520 0.44 -1.5 297,757 - MMJJ_RS01520 0.64 -1.9 297,757 - MMJJ_RS01520 0.64 -1.7 297,779 + MMJJ_RS01520 0.75 -1.4 297,802 - MMJJ_RS01520 0.86 -3.4 297,802 - MMJJ_RS01520 0.86 +0.8 297,807 + MMJJ_RS01520 0.88 -1.1 297,842 - +0.3 297,864 + -3.1 297,901 - -0.5 297,906 - +2.1 297,965 - MMJJ_RS01525 0.13 -1.6 297,965 - MMJJ_RS01525 0.13 -1.5 297,976 - MMJJ_RS01525 0.14 -0.5 297,976 - MMJJ_RS01525 0.14 +0.5 297,977 + MMJJ_RS01525 0.14 -1.8 298,059 - MMJJ_RS01525 0.23 -1.5 298,124 - MMJJ_RS01525 0.30 -0.3 298,129 - MMJJ_RS01525 0.31 +0.5 298,130 + MMJJ_RS01525 0.31 -2.2 298,130 + MMJJ_RS01525 0.31 -0.3 298,135 - MMJJ_RS01525 0.31 -1.3 298,141 - MMJJ_RS01525 0.32 -1.5 298,142 + MMJJ_RS01525 0.32 +3.7 298,142 + MMJJ_RS01525 0.32 -0.1 298,186 - MMJJ_RS01525 0.37 +0.0 298,217 + MMJJ_RS01525 0.40 -2.5 298,218 - MMJJ_RS01525 0.40 -1.0 298,250 + MMJJ_RS01525 0.44 -0.9 298,361 - MMJJ_RS01525 0.56 +0.5 298,362 + MMJJ_RS01525 0.56 +4.5 298,408 - MMJJ_RS01525 0.61 +0.6 298,412 + MMJJ_RS01525 0.62 +2.7 298,412 + MMJJ_RS01525 0.62 +2.8 298,412 + MMJJ_RS01525 0.62 -2.4 298,430 + MMJJ_RS01525 0.64 -4.0 298,435 - MMJJ_RS01525 0.64 +0.0 298,435 - MMJJ_RS01525 0.64 -1.9 298,436 + MMJJ_RS01525 0.64 -3.1 298,436 + MMJJ_RS01525 0.64 -1.5 298,437 - MMJJ_RS01525 0.64 +0.2 298,443 + MMJJ_RS01525 0.65 +2.2 298,500 + MMJJ_RS01525 0.71 +0.7 298,500 + MMJJ_RS01525 0.71 -1.0 298,501 - +0.3 298,501 - MMJJ_RS01525 0.71 -2.4 298,553 - MMJJ_RS01525 0.77 +1.2 298,554 + MMJJ_RS01525 0.77 -0.8 298,555 - MMJJ_RS01525 0.77 +2.2 298,555 - MMJJ_RS01525 0.77 +0.2 298,556 + MMJJ_RS01525 0.77 +2.4 298,558 - MMJJ_RS01525 0.77 +2.8 298,558 - MMJJ_RS01525 0.77 +1.3 298,559 + MMJJ_RS01525 0.78 +1.9 298,561 - MMJJ_RS01525 0.78 +0.8 298,563 - MMJJ_RS01525 0.78 +0.4 298,563 - MMJJ_RS01525 0.78 -0.1 298,656 + MMJJ_RS01525 0.88 +0.3 298,700 - -1.3 298,721 - -2.6 298,727 - +1.7 298,754 - -1.4 298,820 - +1.9 298,820 - +4.8 298,821 + +3.1 298,823 - -0.9 298,826 - +0.3 298,827 + +0.6 298,835 - -1.1 298,836 + -0.8 298,839 - +0.6 298,840 + -3.5 298,843 - -3.0 298,843 - +1.9 298,844 + +0.8 298,844 + -0.7 298,870 + +1.1 298,922 - MMJJ_RS01530 0.28 -3.0 298,923 + MMJJ_RS01530 0.28 -1.3 299,012 - MMJJ_RS01530 0.74 +1.9 299,037 + MMJJ_RS01530 0.87 -2.3 299,063 + +7.7 299,065 - -0.6 299,152 + -0.3 299,155 - -0.5 299,164 - MMJJ_RS01535 0.10 -0.7 299,178 + MMJJ_RS01535 0.12 +3.6 299,212 - MMJJ_RS01535 0.16 -1.5 299,212 - MMJJ_RS01535 0.16 -3.1 299,213 + MMJJ_RS01535 0.16 +1.4 299,214 - MMJJ_RS01535 0.16 -2.7 299,234 + MMJJ_RS01535 0.18 +2.2 299,260 - MMJJ_RS01535 0.21 -1.0 299,274 - MMJJ_RS01535 0.23 +2.2 299,274 - MMJJ_RS01535 0.23 +2.0 299,279 - MMJJ_RS01535 0.23 -3.8 299,280 + MMJJ_RS01535 0.23 -0.3 299,304 - MMJJ_RS01535 0.26 -1.2 299,304 - MMJJ_RS01535 0.26 +1.1 299,340 + MMJJ_RS01535 0.30 +1.0 299,344 - MMJJ_RS01535 0.31 -2.9 299,344 - MMJJ_RS01535 0.31 -1.9 299,345 + MMJJ_RS01535 0.31 +0.8 299,345 + MMJJ_RS01535 0.31 -1.0 299,376 + MMJJ_RS01535 0.34 +1.0 299,383 - MMJJ_RS01535 0.35 +2.0 299,384 + MMJJ_RS01535 0.35 -1.6 299,392 - MMJJ_RS01535 0.36 +2.0 299,392 - MMJJ_RS01535 0.36 -0.4 299,399 - MMJJ_RS01535 0.37 +0.3 299,417 - MMJJ_RS01535 0.39 +2.0 299,418 + MMJJ_RS01535 0.39 -1.5 299,475 - MMJJ_RS01535 0.45 -3.6 299,476 + MMJJ_RS01535 0.45 -2.4 299,488 - MMJJ_RS01535 0.47 -2.0 299,489 + MMJJ_RS01535 0.47 +1.6 299,510 - MMJJ_RS01535 0.49 +1.5 299,511 + MMJJ_RS01535 0.49 +1.8 299,513 + MMJJ_RS01535 0.49 +1.2 299,513 + MMJJ_RS01535 0.49 -0.7 299,513 + MMJJ_RS01535 0.49 +0.5 299,603 - MMJJ_RS01535 0.60 -1.3 299,655 + MMJJ_RS01535 0.65 -1.9 299,655 + MMJJ_RS01535 0.65 +1.9 299,656 - MMJJ_RS01535 0.66 +0.5 299,656 - MMJJ_RS01535 0.66 +0.3 299,661 + MMJJ_RS01535 0.66 +2.0 299,676 + MMJJ_RS01535 0.68 +2.1 299,687 + MMJJ_RS01535 0.69 -1.0 299,687 + MMJJ_RS01535 0.69 -0.2 299,700 - MMJJ_RS01535 0.70 +0.4 299,701 + MMJJ_RS01535 0.71 +0.2 299,706 + MMJJ_RS01535 0.71 -0.5 299,706 + MMJJ_RS01535 0.71 -0.7 299,723 - MMJJ_RS01535 0.73 +2.9 299,727 - MMJJ_RS01535 0.74 -1.4 299,728 + MMJJ_RS01535 0.74 +2.1 299,731 + MMJJ_RS01535 0.74 +1.8 299,737 - MMJJ_RS01535 0.75 +0.6 299,737 - MMJJ_RS01535 0.75 -1.2
Or see this region's nucleotide sequence