Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08180 and MMJJ_RS08185 are separated by 42 nucleotides MMJJ_RS08185 and MMJJ_RS08190 are separated by 182 nucleotides MMJJ_RS08190 and MMJJ_RS08195 are separated by 77 nucleotides MMJJ_RS08195 and MMJJ_RS08200 are separated by 103 nucleotides
MMJJ_RS08180: MMJJ_RS08180 - ABC transporter ATP-binding protein, at 1,502,570 to 1,503,334
_RS08180
MMJJ_RS08185: MMJJ_RS08185 - ABC transporter permease, at 1,503,377 to 1,504,138
_RS08185
MMJJ_RS08190: MMJJ_RS08190 - hypothetical protein, at 1,504,321 to 1,504,869
_RS08190
MMJJ_RS08195: MMJJ_RS08195 - DUF4186 domain-containing protein, at 1,504,947 to 1,505,318
_RS08195
MMJJ_RS08200: MMJJ_RS08200 - surface protein, anchor region, at 1,505,422 to 1,507,425
_RS08200
Position (kb)
1504
1505 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1503.335 kb on + strand at 1503.335 kb on + strand at 1503.361 kb on + strand at 1503.362 kb on - strand at 1503.378 kb on - strand at 1503.419 kb on + strand at 1503.420 kb on - strand at 1503.420 kb on - strand at 1503.420 kb on - strand at 1503.452 kb on - strand at 1503.452 kb on - strand at 1503.453 kb on + strand at 1503.453 kb on + strand at 1503.460 kb on - strand, within MMJJ_RS08185 at 1503.460 kb on - strand, within MMJJ_RS08185 at 1503.460 kb on - strand, within MMJJ_RS08185 at 1503.461 kb on + strand, within MMJJ_RS08185 at 1503.461 kb on + strand, within MMJJ_RS08185 at 1503.464 kb on - strand, within MMJJ_RS08185 at 1503.505 kb on + strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.510 kb on - strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.511 kb on + strand, within MMJJ_RS08185 at 1503.523 kb on + strand, within MMJJ_RS08185 at 1503.608 kb on - strand, within MMJJ_RS08185 at 1503.609 kb on + strand, within MMJJ_RS08185 at 1503.609 kb on + strand, within MMJJ_RS08185 at 1503.636 kb on + strand, within MMJJ_RS08185 at 1503.638 kb on - strand, within MMJJ_RS08185 at 1503.639 kb on + strand, within MMJJ_RS08185 at 1503.652 kb on - strand, within MMJJ_RS08185 at 1503.694 kb on - strand, within MMJJ_RS08185 at 1503.694 kb on - strand, within MMJJ_RS08185 at 1503.695 kb on + strand, within MMJJ_RS08185 at 1503.699 kb on + strand, within MMJJ_RS08185 at 1503.704 kb on - strand, within MMJJ_RS08185 at 1503.704 kb on - strand, within MMJJ_RS08185 at 1503.727 kb on - strand, within MMJJ_RS08185 at 1503.728 kb on + strand, within MMJJ_RS08185 at 1503.728 kb on + strand, within MMJJ_RS08185 at 1503.762 kb on + strand, within MMJJ_RS08185 at 1503.763 kb on - strand, within MMJJ_RS08185 at 1503.766 kb on - strand, within MMJJ_RS08185 at 1503.766 kb on - strand, within MMJJ_RS08185 at 1503.766 kb on - strand, within MMJJ_RS08185 at 1503.767 kb on + strand, within MMJJ_RS08185 at 1503.767 kb on + strand, within MMJJ_RS08185 at 1503.859 kb on - strand, within MMJJ_RS08185 at 1503.866 kb on - strand, within MMJJ_RS08185 at 1503.866 kb on - strand, within MMJJ_RS08185 at 1503.876 kb on + strand, within MMJJ_RS08185 at 1503.876 kb on + strand, within MMJJ_RS08185 at 1503.877 kb on - strand, within MMJJ_RS08185 at 1503.878 kb on + strand, within MMJJ_RS08185 at 1503.878 kb on + strand, within MMJJ_RS08185 at 1503.889 kb on + strand, within MMJJ_RS08185 at 1503.906 kb on + strand, within MMJJ_RS08185 at 1503.906 kb on + strand, within MMJJ_RS08185 at 1503.912 kb on + strand, within MMJJ_RS08185 at 1503.955 kb on - strand, within MMJJ_RS08185 at 1503.955 kb on - strand, within MMJJ_RS08185 at 1503.963 kb on + strand, within MMJJ_RS08185 at 1503.967 kb on - strand, within MMJJ_RS08185 at 1503.968 kb on + strand, within MMJJ_RS08185 at 1503.980 kb on - strand, within MMJJ_RS08185 at 1504.067 kb on - strand at 1504.068 kb on + strand at 1504.071 kb on - strand at 1504.072 kb on + strand at 1504.094 kb on - strand at 1504.151 kb on - strand at 1504.151 kb on - strand at 1504.154 kb on - strand at 1504.184 kb on - strand at 1504.185 kb on + strand at 1504.192 kb on + strand at 1504.206 kb on + strand at 1504.206 kb on + strand at 1504.206 kb on + strand at 1504.209 kb on - strand at 1504.210 kb on + strand at 1504.215 kb on - strand at 1504.235 kb on + strand at 1504.277 kb on + strand at 1504.284 kb on - strand at 1504.284 kb on - strand at 1504.291 kb on - strand at 1504.295 kb on - strand at 1504.302 kb on - strand at 1504.323 kb on + strand at 1504.339 kb on - strand at 1504.340 kb on + strand at 1504.534 kb on - strand at 1504.573 kb on + strand, within MMJJ_RS08190 at 1504.622 kb on - strand, within MMJJ_RS08190 at 1504.622 kb on - strand, within MMJJ_RS08190 at 1504.623 kb on + strand at 1504.623 kb on + strand at 1504.627 kb on - strand, within MMJJ_RS08190 at 1504.627 kb on - strand, within MMJJ_RS08190 at 1504.627 kb on - strand, within MMJJ_RS08190 at 1504.628 kb on + strand, within MMJJ_RS08190 at 1504.677 kb on - strand, within MMJJ_RS08190 at 1504.832 kb on + strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.885 kb on - strand at 1504.886 kb on + strand at 1504.886 kb on + strand at 1504.886 kb on + strand at 1504.886 kb on + strand at 1504.893 kb on - strand at 1504.894 kb on + strand at 1504.898 kb on - strand at 1504.898 kb on - strand at 1504.898 kb on - strand at 1504.898 kb on - strand at 1504.899 kb on + strand at 1504.899 kb on + strand at 1504.899 kb on + strand at 1504.917 kb on - strand at 1504.923 kb on - strand at 1504.996 kb on - strand, within MMJJ_RS08195 at 1505.017 kb on + strand, within MMJJ_RS08195 at 1505.040 kb on + strand, within MMJJ_RS08195 at 1505.097 kb on - strand, within MMJJ_RS08195 at 1505.153 kb on - strand, within MMJJ_RS08195 at 1505.195 kb on - strand, within MMJJ_RS08195 at 1505.216 kb on - strand, within MMJJ_RS08195 at 1505.219 kb on + strand, within MMJJ_RS08195 at 1505.219 kb on + strand, within MMJJ_RS08195 at 1505.225 kb on - strand, within MMJJ_RS08195 at 1505.250 kb on + strand, within MMJJ_RS08195 at 1505.256 kb on + strand, within MMJJ_RS08195 at 1505.293 kb on - strand at 1505.313 kb on + strand at 1505.318 kb on - strand at 1505.318 kb on - strand at 1505.362 kb on - strand at 1505.362 kb on - strand at 1505.362 kb on - strand at 1505.378 kb on + strand at 1505.378 kb on + strand at 1505.407 kb on - strand at 1505.412 kb on - strand at 1505.412 kb on - strand at 1505.413 kb on + strand at 1505.414 kb on - strand at 1505.414 kb on - strand at 1505.488 kb on - strand at 1505.489 kb on + strand at 1505.489 kb on + strand at 1505.494 kb on - strand at 1505.495 kb on + strand at 1505.497 kb on - strand at 1505.498 kb on + strand at 1505.516 kb on - strand at 1505.517 kb on + strand at 1505.517 kb on + strand at 1505.517 kb on + strand at 1505.517 kb on + strand at 1505.517 kb on + strand at 1505.517 kb on + strand at 1505.517 kb on + strand at 1505.551 kb on - strand at 1505.551 kb on - strand at 1505.552 kb on + strand at 1505.552 kb on + strand at 1505.554 kb on - strand at 1505.554 kb on - strand at 1505.555 kb on + strand at 1505.555 kb on + strand at 1505.555 kb on + strand at 1505.590 kb on - strand at 1505.590 kb on - strand at 1505.590 kb on - strand at 1505.591 kb on + strand at 1505.591 kb on + strand at 1505.652 kb on - strand, within MMJJ_RS08200 at 1505.653 kb on + strand, within MMJJ_RS08200 at 1505.698 kb on - strand, within MMJJ_RS08200 at 1505.740 kb on - strand, within MMJJ_RS08200 at 1505.741 kb on + strand, within MMJJ_RS08200 at 1505.742 kb on - strand, within MMJJ_RS08200 at 1505.742 kb on - strand, within MMJJ_RS08200 at 1505.868 kb on - strand, within MMJJ_RS08200
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM remove 1,503,335 + -2.2 1,503,335 + -1.1 1,503,361 + -0.1 1,503,362 - -0.2 1,503,378 - +0.1 1,503,419 + -2.0 1,503,420 - -0.3 1,503,420 - -1.8 1,503,420 - +0.4 1,503,452 - -0.5 1,503,452 - +0.7 1,503,453 + -0.5 1,503,453 + -2.2 1,503,460 - MMJJ_RS08185 0.11 +1.8 1,503,460 - MMJJ_RS08185 0.11 +1.9 1,503,460 - MMJJ_RS08185 0.11 -0.6 1,503,461 + MMJJ_RS08185 0.11 -1.8 1,503,461 + MMJJ_RS08185 0.11 -0.7 1,503,464 - MMJJ_RS08185 0.11 -0.4 1,503,505 + MMJJ_RS08185 0.17 -3.6 1,503,510 - MMJJ_RS08185 0.17 -2.0 1,503,510 - MMJJ_RS08185 0.17 +0.9 1,503,510 - MMJJ_RS08185 0.17 -3.9 1,503,510 - MMJJ_RS08185 0.17 +1.4 1,503,510 - MMJJ_RS08185 0.17 -3.8 1,503,510 - MMJJ_RS08185 0.17 -1.6 1,503,510 - MMJJ_RS08185 0.17 -3.9 1,503,510 - MMJJ_RS08185 0.17 -3.0 1,503,510 - MMJJ_RS08185 0.17 -0.9 1,503,510 - MMJJ_RS08185 0.17 -0.1 1,503,511 + MMJJ_RS08185 0.18 +0.7 1,503,511 + MMJJ_RS08185 0.18 -0.6 1,503,511 + MMJJ_RS08185 0.18 +1.7 1,503,511 + MMJJ_RS08185 0.18 +1.0 1,503,511 + MMJJ_RS08185 0.18 +0.3 1,503,511 + MMJJ_RS08185 0.18 +0.8 1,503,511 + MMJJ_RS08185 0.18 -2.7 1,503,511 + MMJJ_RS08185 0.18 +0.9 1,503,511 + MMJJ_RS08185 0.18 -1.1 1,503,511 + MMJJ_RS08185 0.18 +2.7 1,503,511 + MMJJ_RS08185 0.18 -1.5 1,503,523 + MMJJ_RS08185 0.19 -1.0 1,503,608 - MMJJ_RS08185 0.30 -3.6 1,503,609 + MMJJ_RS08185 0.30 -3.2 1,503,609 + MMJJ_RS08185 0.30 -0.2 1,503,636 + MMJJ_RS08185 0.34 +1.6 1,503,638 - MMJJ_RS08185 0.34 +1.2 1,503,639 + MMJJ_RS08185 0.34 -0.4 1,503,652 - MMJJ_RS08185 0.36 -1.3 1,503,694 - MMJJ_RS08185 0.42 -3.6 1,503,694 - MMJJ_RS08185 0.42 +0.4 1,503,695 + MMJJ_RS08185 0.42 -0.5 1,503,699 + MMJJ_RS08185 0.42 +1.5 1,503,704 - MMJJ_RS08185 0.43 +1.5 1,503,704 - MMJJ_RS08185 0.43 +2.7 1,503,727 - MMJJ_RS08185 0.46 +0.4 1,503,728 + MMJJ_RS08185 0.46 -2.4 1,503,728 + MMJJ_RS08185 0.46 -0.3 1,503,762 + MMJJ_RS08185 0.51 -3.3 1,503,763 - MMJJ_RS08185 0.51 -1.6 1,503,766 - MMJJ_RS08185 0.51 -0.6 1,503,766 - MMJJ_RS08185 0.51 +0.4 1,503,766 - MMJJ_RS08185 0.51 -0.9 1,503,767 + MMJJ_RS08185 0.51 -0.0 1,503,767 + MMJJ_RS08185 0.51 -1.6 1,503,859 - MMJJ_RS08185 0.63 -2.8 1,503,866 - MMJJ_RS08185 0.64 -2.4 1,503,866 - MMJJ_RS08185 0.64 +0.3 1,503,876 + MMJJ_RS08185 0.65 -0.1 1,503,876 + MMJJ_RS08185 0.65 -1.7 1,503,877 - MMJJ_RS08185 0.66 +0.7 1,503,878 + MMJJ_RS08185 0.66 +1.3 1,503,878 + MMJJ_RS08185 0.66 +0.4 1,503,889 + MMJJ_RS08185 0.67 -2.2 1,503,906 + MMJJ_RS08185 0.69 -0.5 1,503,906 + MMJJ_RS08185 0.69 -3.2 1,503,912 + MMJJ_RS08185 0.70 -2.3 1,503,955 - MMJJ_RS08185 0.76 -1.6 1,503,955 - MMJJ_RS08185 0.76 +0.4 1,503,963 + MMJJ_RS08185 0.77 +0.4 1,503,967 - MMJJ_RS08185 0.77 -0.8 1,503,968 + MMJJ_RS08185 0.78 +2.2 1,503,980 - MMJJ_RS08185 0.79 -1.3 1,504,067 - -2.6 1,504,068 + +1.4 1,504,071 - -2.6 1,504,072 + -0.8 1,504,094 - +0.9 1,504,151 - -0.1 1,504,151 - -4.2 1,504,154 - -1.4 1,504,184 - -1.4 1,504,185 + -0.1 1,504,192 + -1.3 1,504,206 + -2.5 1,504,206 + -0.4 1,504,206 + -0.4 1,504,209 - +0.2 1,504,210 + +1.9 1,504,215 - +2.2 1,504,235 + +2.1 1,504,277 + +0.6 1,504,284 - -1.8 1,504,284 - -1.4 1,504,291 - -0.5 1,504,295 - +0.1 1,504,302 - -1.7 1,504,323 + +0.8 1,504,339 - -2.4 1,504,340 + -4.0 1,504,534 - +1.4 1,504,573 + MMJJ_RS08190 0.46 -1.0 1,504,622 - MMJJ_RS08190 0.55 -2.1 1,504,622 - MMJJ_RS08190 0.55 -2.4 1,504,623 + -0.2 1,504,623 + +1.4 1,504,627 - MMJJ_RS08190 0.56 -1.6 1,504,627 - MMJJ_RS08190 0.56 -0.9 1,504,627 - MMJJ_RS08190 0.56 -0.9 1,504,628 + MMJJ_RS08190 0.56 -1.2 1,504,677 - MMJJ_RS08190 0.65 -0.9 1,504,832 + -2.1 1,504,885 - +0.6 1,504,885 - +1.1 1,504,885 - -1.2 1,504,885 - +2.5 1,504,885 - -2.7 1,504,885 - -2.6 1,504,885 - -0.4 1,504,885 - +3.5 1,504,886 + -1.9 1,504,886 + -1.5 1,504,886 + +0.7 1,504,886 + +0.9 1,504,893 - +3.7 1,504,894 + -2.4 1,504,898 - +1.2 1,504,898 - +0.4 1,504,898 - +1.4 1,504,898 - +1.3 1,504,899 + -1.7 1,504,899 + +4.2 1,504,899 + -0.3 1,504,917 - +2.4 1,504,923 - +1.9 1,504,996 - MMJJ_RS08195 0.13 -1.2 1,505,017 + MMJJ_RS08195 0.19 +0.5 1,505,040 + MMJJ_RS08195 0.25 -3.0 1,505,097 - MMJJ_RS08195 0.40 +1.7 1,505,153 - MMJJ_RS08195 0.55 +1.6 1,505,195 - MMJJ_RS08195 0.67 +1.4 1,505,216 - MMJJ_RS08195 0.72 +0.3 1,505,219 + MMJJ_RS08195 0.73 +0.9 1,505,219 + MMJJ_RS08195 0.73 -0.6 1,505,225 - MMJJ_RS08195 0.75 +0.7 1,505,250 + MMJJ_RS08195 0.81 +2.1 1,505,256 + MMJJ_RS08195 0.83 +2.4 1,505,293 - -3.5 1,505,313 + -0.6 1,505,318 - +1.0 1,505,318 - -0.6 1,505,362 - -3.4 1,505,362 - -2.8 1,505,362 - -4.3 1,505,378 + +1.9 1,505,378 + +0.8 1,505,407 - -0.6 1,505,412 - -0.4 1,505,412 - +1.9 1,505,413 + +0.1 1,505,414 - +0.7 1,505,414 - -0.3 1,505,488 - -2.5 1,505,489 + +3.0 1,505,489 + -1.2 1,505,494 - +2.8 1,505,495 + +1.2 1,505,497 - -0.9 1,505,498 + -2.5 1,505,516 - -0.9 1,505,517 + +1.3 1,505,517 + -0.2 1,505,517 + +2.7 1,505,517 + +2.3 1,505,517 + -2.2 1,505,517 + -2.4 1,505,517 + +1.4 1,505,551 - +2.5 1,505,551 - -1.4 1,505,552 + -2.2 1,505,552 + -1.0 1,505,554 - +1.7 1,505,554 - -3.9 1,505,555 + +2.4 1,505,555 + -0.0 1,505,555 + -1.2 1,505,590 - +1.1 1,505,590 - -0.1 1,505,590 - +1.5 1,505,591 + +0.4 1,505,591 + -3.9 1,505,652 - MMJJ_RS08200 0.11 +3.1 1,505,653 + MMJJ_RS08200 0.12 +0.0 1,505,698 - MMJJ_RS08200 0.14 +2.6 1,505,740 - MMJJ_RS08200 0.16 -3.3 1,505,741 + MMJJ_RS08200 0.16 +2.5 1,505,742 - MMJJ_RS08200 0.16 -2.1 1,505,742 - MMJJ_RS08200 0.16 +1.9 1,505,868 - MMJJ_RS08200 0.22 -1.4
Or see this region's nucleotide sequence