Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS07970

Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS07960 and MMJJ_RS07965 are separated by 10 nucleotidesMMJJ_RS07965 and MMJJ_RS07970 are separated by 5 nucleotidesMMJJ_RS07970 and MMJJ_RS07975 are separated by 27 nucleotidesMMJJ_RS07975 and MMJJ_RS07980 overlap by 4 nucleotides MMJJ_RS07960: MMJJ_RS07960 - quinolinate synthase NadA, at 1,459,928 to 1,460,845 _RS07960 MMJJ_RS07965: MMJJ_RS07965 - hypothetical protein, at 1,460,856 to 1,461,215 _RS07965 MMJJ_RS07970: MMJJ_RS07970 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,461,221 to 1,462,363 _RS07970 MMJJ_RS07975: MMJJ_RS07975 - ATP-dependent sacrificial sulfur transferase LarE, at 1,462,391 to 1,463,167 _RS07975 MMJJ_RS07980: MMJJ_RS07980 - biotin synthase BioB, at 1,463,164 to 1,464,147 _RS07980 Position (kb) 1461 1462 1463Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1460.264 kb on + strand, within MMJJ_RS07960at 1460.512 kb on + strand, within MMJJ_RS07960at 1460.740 kb on + strand, within MMJJ_RS07960at 1460.872 kb on - strandat 1460.873 kb on + strandat 1460.873 kb on + strandat 1460.875 kb on - strandat 1460.917 kb on - strand, within MMJJ_RS07965at 1460.918 kb on + strand, within MMJJ_RS07965at 1460.920 kb on - strand, within MMJJ_RS07965at 1460.950 kb on + strand, within MMJJ_RS07965at 1460.953 kb on - strand, within MMJJ_RS07965at 1460.955 kb on - strand, within MMJJ_RS07965at 1460.956 kb on + strand, within MMJJ_RS07965at 1460.956 kb on + strand, within MMJJ_RS07965at 1461.040 kb on - strand, within MMJJ_RS07965at 1461.051 kb on - strand, within MMJJ_RS07965at 1461.051 kb on - strand, within MMJJ_RS07965at 1461.074 kb on + strand, within MMJJ_RS07965at 1461.207 kb on + strandat 1462.053 kb on + strand, within MMJJ_RS07970at 1462.093 kb on + strand, within MMJJ_RS07970at 1462.246 kb on + strand, within MMJJ_RS07970at 1462.299 kb on + strandat 1462.327 kb on - strandat 1462.345 kb on - strandat 1462.364 kb on + strandat 1462.388 kb on - strandat 1462.392 kb on - strandat 1462.392 kb on - strandat 1462.393 kb on + strandat 1462.412 kb on - strandat 1462.412 kb on - strandat 1462.413 kb on + strandat 1462.431 kb on + strandat 1462.447 kb on - strandat 1462.451 kb on - strandat 1462.451 kb on - strandat 1462.454 kb on - strandat 1462.454 kb on - strandat 1462.455 kb on + strandat 1462.499 kb on + strand, within MMJJ_RS07975at 1462.510 kb on - strand, within MMJJ_RS07975at 1462.545 kb on - strand, within MMJJ_RS07975at 1462.572 kb on + strand, within MMJJ_RS07975at 1462.603 kb on - strand, within MMJJ_RS07975at 1462.604 kb on + strand, within MMJJ_RS07975at 1462.651 kb on - strand, within MMJJ_RS07975at 1462.652 kb on + strand, within MMJJ_RS07975at 1462.680 kb on - strand, within MMJJ_RS07975at 1462.681 kb on + strand, within MMJJ_RS07975at 1462.754 kb on + strand, within MMJJ_RS07975at 1462.764 kb on + strand, within MMJJ_RS07975at 1462.784 kb on - strand, within MMJJ_RS07975at 1462.786 kb on - strand, within MMJJ_RS07975at 1462.821 kb on - strand, within MMJJ_RS07975at 1462.821 kb on - strand, within MMJJ_RS07975at 1462.846 kb on - strand, within MMJJ_RS07975at 1463.059 kb on - strand, within MMJJ_RS07975at 1463.060 kb on + strand, within MMJJ_RS07975at 1463.060 kb on + strand, within MMJJ_RS07975at 1463.060 kb on + strand, within MMJJ_RS07975at 1463.234 kb on - strandat 1463.352 kb on - strand, within MMJJ_RS07980at 1463.353 kb on + strand, within MMJJ_RS07980at 1463.356 kb on - strand, within MMJJ_RS07980

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM
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1,460,264 + MMJJ_RS07960 0.37 +0.4
1,460,512 + MMJJ_RS07960 0.64 -1.7
1,460,740 + MMJJ_RS07960 0.88 -0.9
1,460,872 - -2.7
1,460,873 + +1.0
1,460,873 + +2.2
1,460,875 - -1.8
1,460,917 - MMJJ_RS07965 0.17 +0.1
1,460,918 + MMJJ_RS07965 0.17 -1.6
1,460,920 - MMJJ_RS07965 0.18 -0.6
1,460,950 + MMJJ_RS07965 0.26 +1.4
1,460,953 - MMJJ_RS07965 0.27 +2.7
1,460,955 - MMJJ_RS07965 0.28 +0.1
1,460,956 + MMJJ_RS07965 0.28 -2.8
1,460,956 + MMJJ_RS07965 0.28 -0.2
1,461,040 - MMJJ_RS07965 0.51 -1.1
1,461,051 - MMJJ_RS07965 0.54 -1.7
1,461,051 - MMJJ_RS07965 0.54 -1.7
1,461,074 + MMJJ_RS07965 0.61 +2.2
1,461,207 + +2.8
1,462,053 + MMJJ_RS07970 0.73 -1.1
1,462,093 + MMJJ_RS07970 0.76 -0.6
1,462,246 + MMJJ_RS07970 0.90 -1.4
1,462,299 + +0.4
1,462,327 - -1.1
1,462,345 - -1.1
1,462,364 + -1.1
1,462,388 - +1.0
1,462,392 - -0.4
1,462,392 - +0.2
1,462,393 + +0.3
1,462,412 - +0.3
1,462,412 - +0.5
1,462,413 + -1.1
1,462,431 + -1.9
1,462,447 - -1.7
1,462,451 - -1.6
1,462,451 - +0.4
1,462,454 - -2.1
1,462,454 - +2.2
1,462,455 + -2.0
1,462,499 + MMJJ_RS07975 0.14 -1.3
1,462,510 - MMJJ_RS07975 0.15 -1.1
1,462,545 - MMJJ_RS07975 0.20 -0.3
1,462,572 + MMJJ_RS07975 0.23 -1.7
1,462,603 - MMJJ_RS07975 0.27 -1.9
1,462,604 + MMJJ_RS07975 0.27 +0.4
1,462,651 - MMJJ_RS07975 0.33 -0.6
1,462,652 + MMJJ_RS07975 0.34 -1.0
1,462,680 - MMJJ_RS07975 0.37 +0.4
1,462,681 + MMJJ_RS07975 0.37 -1.4
1,462,754 + MMJJ_RS07975 0.47 -0.1
1,462,764 + MMJJ_RS07975 0.48 -3.0
1,462,784 - MMJJ_RS07975 0.51 -4.4
1,462,786 - MMJJ_RS07975 0.51 -3.8
1,462,821 - MMJJ_RS07975 0.55 -3.7
1,462,821 - MMJJ_RS07975 0.55 -4.1
1,462,846 - MMJJ_RS07975 0.59 -2.7
1,463,059 - MMJJ_RS07975 0.86 -0.7
1,463,060 + MMJJ_RS07975 0.86 -2.6
1,463,060 + MMJJ_RS07975 0.86 -3.8
1,463,060 + MMJJ_RS07975 0.86 -1.7
1,463,234 - -2.6
1,463,352 - MMJJ_RS07980 0.19 +0.9
1,463,353 + MMJJ_RS07980 0.19 -1.7
1,463,356 - MMJJ_RS07980 0.20 +1.4

Or see this region's nucleotide sequence