Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07425 and MMJJ_RS07430 are separated by 17 nucleotides MMJJ_RS07430 and MMJJ_RS07435 are separated by 9 nucleotides MMJJ_RS07435 and MMJJ_RS07440 are separated by 13 nucleotides
MMJJ_RS07425: MMJJ_RS07425 - MBL fold metallo-hydrolase, at 1,362,762 to 1,363,562
_RS07425
MMJJ_RS07430: MMJJ_RS07430 - ribonuclease VapC, at 1,363,580 to 1,363,975
_RS07430
MMJJ_RS07435: MMJJ_RS07435 - SMC family ATPase, at 1,363,985 to 1,366,966
_RS07435
MMJJ_RS07440: MMJJ_RS07440 - metallophosphoesterase, at 1,366,980 to 1,368,098
_RS07440
Position (kb)
1363
1364
1365
1366
1367 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1363.093 kb on + strand, within MMJJ_RS07425 at 1363.145 kb on + strand, within MMJJ_RS07425 at 1363.145 kb on + strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.166 kb on + strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.234 kb on - strand, within MMJJ_RS07425 at 1363.234 kb on - strand, within MMJJ_RS07425 at 1363.244 kb on - strand, within MMJJ_RS07425 at 1363.260 kb on - strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.274 kb on + strand, within MMJJ_RS07425 at 1363.315 kb on - strand, within MMJJ_RS07425 at 1363.366 kb on - strand, within MMJJ_RS07425 at 1363.366 kb on - strand, within MMJJ_RS07425 at 1363.367 kb on + strand, within MMJJ_RS07425 at 1363.367 kb on + strand, within MMJJ_RS07425 at 1363.378 kb on - strand, within MMJJ_RS07425 at 1363.417 kb on + strand, within MMJJ_RS07425 at 1363.464 kb on + strand, within MMJJ_RS07425 at 1363.482 kb on + strand, within MMJJ_RS07425 at 1363.529 kb on - strand at 1363.548 kb on - strand at 1363.550 kb on - strand at 1363.550 kb on - strand at 1363.553 kb on - strand at 1363.557 kb on - strand at 1363.582 kb on + strand at 1363.587 kb on + strand at 1363.691 kb on + strand, within MMJJ_RS07430 at 1363.691 kb on + strand, within MMJJ_RS07430 at 1363.714 kb on + strand, within MMJJ_RS07430 at 1363.868 kb on + strand, within MMJJ_RS07430 at 1363.870 kb on + strand, within MMJJ_RS07430 at 1363.871 kb on - strand, within MMJJ_RS07430 at 1363.901 kb on - strand, within MMJJ_RS07430 at 1363.986 kb on - strand at 1363.987 kb on + strand at 1363.987 kb on + strand at 1363.998 kb on + strand at 1364.023 kb on - strand at 1364.031 kb on + strand at 1364.038 kb on - strand at 1364.038 kb on - strand at 1364.038 kb on - strand at 1364.071 kb on - strand at 1364.072 kb on + strand at 1364.148 kb on - strand at 1364.174 kb on + strand at 1364.186 kb on + strand at 1364.188 kb on - strand at 1364.188 kb on - strand at 1364.188 kb on - strand at 1364.201 kb on + strand at 1364.229 kb on + strand at 1364.300 kb on - strand, within MMJJ_RS07435 at 1364.301 kb on + strand, within MMJJ_RS07435 at 1364.373 kb on - strand, within MMJJ_RS07435 at 1364.373 kb on - strand, within MMJJ_RS07435 at 1364.431 kb on - strand, within MMJJ_RS07435 at 1364.507 kb on - strand, within MMJJ_RS07435 at 1364.512 kb on + strand, within MMJJ_RS07435 at 1364.519 kb on + strand, within MMJJ_RS07435 at 1364.592 kb on + strand, within MMJJ_RS07435 at 1364.760 kb on - strand, within MMJJ_RS07435 at 1364.761 kb on + strand, within MMJJ_RS07435 at 1364.800 kb on - strand, within MMJJ_RS07435 at 1364.874 kb on - strand, within MMJJ_RS07435 at 1364.874 kb on - strand, within MMJJ_RS07435 at 1364.875 kb on + strand, within MMJJ_RS07435 at 1364.875 kb on + strand, within MMJJ_RS07435 at 1364.875 kb on + strand, within MMJJ_RS07435 at 1365.150 kb on + strand, within MMJJ_RS07435 at 1365.170 kb on - strand, within MMJJ_RS07435 at 1365.170 kb on - strand, within MMJJ_RS07435 at 1365.170 kb on - strand, within MMJJ_RS07435 at 1365.170 kb on - strand, within MMJJ_RS07435 at 1365.171 kb on + strand, within MMJJ_RS07435 at 1365.212 kb on - strand, within MMJJ_RS07435 at 1365.221 kb on - strand, within MMJJ_RS07435 at 1365.232 kb on - strand, within MMJJ_RS07435 at 1365.268 kb on + strand, within MMJJ_RS07435 at 1365.285 kb on + strand, within MMJJ_RS07435 at 1365.328 kb on - strand, within MMJJ_RS07435 at 1365.353 kb on - strand, within MMJJ_RS07435 at 1365.421 kb on + strand, within MMJJ_RS07435 at 1365.518 kb on + strand at 1365.532 kb on - strand, within MMJJ_RS07435 at 1365.533 kb on + strand, within MMJJ_RS07435 at 1365.799 kb on - strand, within MMJJ_RS07435 at 1365.799 kb on - strand, within MMJJ_RS07435 at 1365.986 kb on + strand, within MMJJ_RS07435 at 1365.990 kb on + strand, within MMJJ_RS07435 at 1366.030 kb on - strand, within MMJJ_RS07435 at 1366.030 kb on - strand, within MMJJ_RS07435 at 1366.062 kb on + strand, within MMJJ_RS07435 at 1366.133 kb on - strand, within MMJJ_RS07435 at 1366.157 kb on - strand, within MMJJ_RS07435 at 1366.217 kb on + strand, within MMJJ_RS07435 at 1366.217 kb on - strand, within MMJJ_RS07435 at 1366.310 kb on - strand, within MMJJ_RS07435 at 1366.374 kb on + strand, within MMJJ_RS07435 at 1366.413 kb on + strand, within MMJJ_RS07435 at 1366.413 kb on + strand, within MMJJ_RS07435 at 1366.446 kb on - strand, within MMJJ_RS07435 at 1366.457 kb on - strand, within MMJJ_RS07435 at 1366.458 kb on + strand, within MMJJ_RS07435 at 1366.528 kb on - strand, within MMJJ_RS07435 at 1366.528 kb on - strand, within MMJJ_RS07435 at 1366.576 kb on - strand, within MMJJ_RS07435 at 1366.576 kb on - strand, within MMJJ_RS07435 at 1366.578 kb on - strand, within MMJJ_RS07435 at 1366.578 kb on - strand, within MMJJ_RS07435 at 1366.578 kb on - strand, within MMJJ_RS07435 at 1366.579 kb on + strand, within MMJJ_RS07435 at 1366.580 kb on - strand, within MMJJ_RS07435 at 1366.595 kb on - strand, within MMJJ_RS07435 at 1366.678 kb on + strand at 1366.725 kb on - strand at 1366.725 kb on - strand at 1366.726 kb on + strand at 1366.729 kb on - strand at 1366.730 kb on + strand at 1366.985 kb on + strand at 1367.049 kb on + strand at 1367.071 kb on + strand at 1367.091 kb on + strand at 1367.093 kb on - strand, within MMJJ_RS07440 at 1367.245 kb on + strand, within MMJJ_RS07440 at 1367.288 kb on - strand, within MMJJ_RS07440 at 1367.289 kb on + strand, within MMJJ_RS07440 at 1367.289 kb on + strand, within MMJJ_RS07440 at 1367.375 kb on - strand, within MMJJ_RS07440 at 1367.376 kb on + strand, within MMJJ_RS07440 at 1367.447 kb on - strand, within MMJJ_RS07440 at 1367.477 kb on + strand, within MMJJ_RS07440 at 1367.512 kb on + strand, within MMJJ_RS07440 at 1367.544 kb on + strand, within MMJJ_RS07440 at 1367.545 kb on - strand, within MMJJ_RS07440 at 1367.546 kb on + strand, within MMJJ_RS07440 at 1367.546 kb on + strand, within MMJJ_RS07440 at 1367.546 kb on + strand, within MMJJ_RS07440 at 1367.546 kb on + strand, within MMJJ_RS07440 at 1367.546 kb on + strand, within MMJJ_RS07440 at 1367.550 kb on + strand, within MMJJ_RS07440 at 1367.622 kb on - strand, within MMJJ_RS07440 at 1367.622 kb on - strand, within MMJJ_RS07440 at 1367.680 kb on - strand, within MMJJ_RS07440 at 1367.711 kb on + strand, within MMJJ_RS07440 at 1367.711 kb on + strand, within MMJJ_RS07440 at 1367.772 kb on - strand, within MMJJ_RS07440 at 1367.776 kb on + strand, within MMJJ_RS07440 at 1367.916 kb on - strand, within MMJJ_RS07440 at 1367.949 kb on - strand, within MMJJ_RS07440 at 1367.950 kb on + strand, within MMJJ_RS07440 at 1367.950 kb on + strand, within MMJJ_RS07440
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM remove 1,363,093 + MMJJ_RS07425 0.41 -1.1 1,363,145 + MMJJ_RS07425 0.48 +0.8 1,363,145 + MMJJ_RS07425 0.48 -2.3 1,363,165 - MMJJ_RS07425 0.50 -1.0 1,363,165 - MMJJ_RS07425 0.50 -0.8 1,363,165 - MMJJ_RS07425 0.50 -1.8 1,363,166 + MMJJ_RS07425 0.50 -2.1 1,363,204 - MMJJ_RS07425 0.55 -2.5 1,363,204 - MMJJ_RS07425 0.55 -2.0 1,363,204 - MMJJ_RS07425 0.55 +0.1 1,363,212 + MMJJ_RS07425 0.56 -0.8 1,363,212 + MMJJ_RS07425 0.56 -0.5 1,363,212 + MMJJ_RS07425 0.56 +0.1 1,363,234 - MMJJ_RS07425 0.59 +1.4 1,363,234 - MMJJ_RS07425 0.59 -2.8 1,363,244 - MMJJ_RS07425 0.60 +1.2 1,363,260 - MMJJ_RS07425 0.62 -0.8 1,363,261 + MMJJ_RS07425 0.62 -2.4 1,363,261 + MMJJ_RS07425 0.62 -3.1 1,363,261 + MMJJ_RS07425 0.62 -2.8 1,363,274 + MMJJ_RS07425 0.64 -0.8 1,363,315 - MMJJ_RS07425 0.69 -0.5 1,363,366 - MMJJ_RS07425 0.75 -1.0 1,363,366 - MMJJ_RS07425 0.75 +0.1 1,363,367 + MMJJ_RS07425 0.76 -4.3 1,363,367 + MMJJ_RS07425 0.76 -0.5 1,363,378 - MMJJ_RS07425 0.77 -2.4 1,363,417 + MMJJ_RS07425 0.82 +0.3 1,363,464 + MMJJ_RS07425 0.88 +0.9 1,363,482 + MMJJ_RS07425 0.90 +0.4 1,363,529 - -1.4 1,363,548 - +3.2 1,363,550 - +2.4 1,363,550 - +2.0 1,363,553 - +2.8 1,363,557 - -4.1 1,363,582 + +0.1 1,363,587 + -2.7 1,363,691 + MMJJ_RS07430 0.28 -0.1 1,363,691 + MMJJ_RS07430 0.28 +2.5 1,363,714 + MMJJ_RS07430 0.34 -1.7 1,363,868 + MMJJ_RS07430 0.73 -2.5 1,363,870 + MMJJ_RS07430 0.73 +1.1 1,363,871 - MMJJ_RS07430 0.73 -2.2 1,363,901 - MMJJ_RS07430 0.81 +1.5 1,363,986 - +2.1 1,363,987 + +2.4 1,363,987 + -2.8 1,363,998 + +0.1 1,364,023 - +3.3 1,364,031 + +1.2 1,364,038 - +1.1 1,364,038 - +5.0 1,364,038 - -0.5 1,364,071 - -2.7 1,364,072 + +1.2 1,364,148 - +1.8 1,364,174 + -0.4 1,364,186 + +4.5 1,364,188 - +3.3 1,364,188 - +1.8 1,364,188 - +2.5 1,364,201 + +0.7 1,364,229 + +2.6 1,364,300 - MMJJ_RS07435 0.11 -0.9 1,364,301 + MMJJ_RS07435 0.11 +1.4 1,364,373 - MMJJ_RS07435 0.13 +1.7 1,364,373 - MMJJ_RS07435 0.13 +2.0 1,364,431 - MMJJ_RS07435 0.15 -2.0 1,364,507 - MMJJ_RS07435 0.18 -3.0 1,364,512 + MMJJ_RS07435 0.18 +1.0 1,364,519 + MMJJ_RS07435 0.18 +2.1 1,364,592 + MMJJ_RS07435 0.20 +2.3 1,364,760 - MMJJ_RS07435 0.26 -1.8 1,364,761 + MMJJ_RS07435 0.26 +2.8 1,364,800 - MMJJ_RS07435 0.27 +3.9 1,364,874 - MMJJ_RS07435 0.30 +2.2 1,364,874 - MMJJ_RS07435 0.30 +0.8 1,364,875 + MMJJ_RS07435 0.30 +2.9 1,364,875 + MMJJ_RS07435 0.30 +0.9 1,364,875 + MMJJ_RS07435 0.30 +0.2 1,365,150 + MMJJ_RS07435 0.39 +0.2 1,365,170 - MMJJ_RS07435 0.40 +2.3 1,365,170 - MMJJ_RS07435 0.40 -1.1 1,365,170 - MMJJ_RS07435 0.40 +2.4 1,365,170 - MMJJ_RS07435 0.40 -3.0 1,365,171 + MMJJ_RS07435 0.40 +1.7 1,365,212 - MMJJ_RS07435 0.41 -0.9 1,365,221 - MMJJ_RS07435 0.41 +2.6 1,365,232 - MMJJ_RS07435 0.42 -1.1 1,365,268 + MMJJ_RS07435 0.43 +4.5 1,365,285 + MMJJ_RS07435 0.44 +2.3 1,365,328 - MMJJ_RS07435 0.45 -0.9 1,365,353 - MMJJ_RS07435 0.46 +3.0 1,365,421 + MMJJ_RS07435 0.48 -1.3 1,365,518 + +1.5 1,365,532 - MMJJ_RS07435 0.52 -2.2 1,365,533 + MMJJ_RS07435 0.52 -0.5 1,365,799 - MMJJ_RS07435 0.61 +1.1 1,365,799 - MMJJ_RS07435 0.61 +1.7 1,365,986 + MMJJ_RS07435 0.67 +2.8 1,365,990 + MMJJ_RS07435 0.67 +1.0 1,366,030 - MMJJ_RS07435 0.69 +0.8 1,366,030 - MMJJ_RS07435 0.69 +2.5 1,366,062 + MMJJ_RS07435 0.70 +1.5 1,366,133 - MMJJ_RS07435 0.72 -0.6 1,366,157 - MMJJ_RS07435 0.73 +0.4 1,366,217 + MMJJ_RS07435 0.75 -0.5 1,366,217 - MMJJ_RS07435 0.75 +1.7 1,366,310 - MMJJ_RS07435 0.78 -0.5 1,366,374 + MMJJ_RS07435 0.80 +1.0 1,366,413 + MMJJ_RS07435 0.81 +1.1 1,366,413 + MMJJ_RS07435 0.81 +2.0 1,366,446 - MMJJ_RS07435 0.83 +2.9 1,366,457 - MMJJ_RS07435 0.83 -2.2 1,366,458 + MMJJ_RS07435 0.83 +2.6 1,366,528 - MMJJ_RS07435 0.85 -1.1 1,366,528 - MMJJ_RS07435 0.85 -0.7 1,366,576 - MMJJ_RS07435 0.87 +0.9 1,366,576 - MMJJ_RS07435 0.87 +0.1 1,366,578 - MMJJ_RS07435 0.87 -1.6 1,366,578 - MMJJ_RS07435 0.87 +0.7 1,366,578 - MMJJ_RS07435 0.87 -0.5 1,366,579 + MMJJ_RS07435 0.87 +2.9 1,366,580 - MMJJ_RS07435 0.87 +2.4 1,366,595 - MMJJ_RS07435 0.88 -2.9 1,366,678 + +2.1 1,366,725 - +0.4 1,366,725 - -0.1 1,366,726 + +0.5 1,366,729 - +2.3 1,366,730 + +1.3 1,366,985 + +2.6 1,367,049 + +0.5 1,367,071 + +2.2 1,367,091 + -1.1 1,367,093 - MMJJ_RS07440 0.10 +0.5 1,367,245 + MMJJ_RS07440 0.24 -0.6 1,367,288 - MMJJ_RS07440 0.28 -0.3 1,367,289 + MMJJ_RS07440 0.28 -4.1 1,367,289 + MMJJ_RS07440 0.28 +1.9 1,367,375 - MMJJ_RS07440 0.35 -2.9 1,367,376 + MMJJ_RS07440 0.35 -0.8 1,367,447 - MMJJ_RS07440 0.42 -1.3 1,367,477 + MMJJ_RS07440 0.44 -1.5 1,367,512 + MMJJ_RS07440 0.48 +0.9 1,367,544 + MMJJ_RS07440 0.50 +1.5 1,367,545 - MMJJ_RS07440 0.50 -0.7 1,367,546 + MMJJ_RS07440 0.51 -3.7 1,367,546 + MMJJ_RS07440 0.51 +5.3 1,367,546 + MMJJ_RS07440 0.51 -0.7 1,367,546 + MMJJ_RS07440 0.51 -1.8 1,367,546 + MMJJ_RS07440 0.51 -0.5 1,367,550 + MMJJ_RS07440 0.51 -3.2 1,367,622 - MMJJ_RS07440 0.57 +0.1 1,367,622 - MMJJ_RS07440 0.57 +2.2 1,367,680 - MMJJ_RS07440 0.63 +1.7 1,367,711 + MMJJ_RS07440 0.65 +3.8 1,367,711 + MMJJ_RS07440 0.65 -1.5 1,367,772 - MMJJ_RS07440 0.71 +1.9 1,367,776 + MMJJ_RS07440 0.71 +1.2 1,367,916 - MMJJ_RS07440 0.84 +2.6 1,367,949 - MMJJ_RS07440 0.87 +1.4 1,367,950 + MMJJ_RS07440 0.87 -0.1 1,367,950 + MMJJ_RS07440 0.87 +2.5
Or see this region's nucleotide sequence