Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05655 and MMJJ_RS05660 are separated by 1 nucleotides MMJJ_RS05660 and MMJJ_RS05665 are separated by 12 nucleotides MMJJ_RS05665 and MMJJ_RS05670 are separated by 11 nucleotides MMJJ_RS05670 and MMJJ_RS05675 are separated by 15 nucleotides
MMJJ_RS05655: MMJJ_RS05655 - HisA/HisF family protein, at 1,055,658 to 1,056,326
_RS05655
MMJJ_RS05660: MMJJ_RS05660 - sulfopyruvate decarboxylase subunit beta, at 1,056,328 to 1,056,885
_RS05660
MMJJ_RS05665: MMJJ_RS05665 - hypothetical protein, at 1,056,898 to 1,057,581
_RS05665
MMJJ_RS05670: MMJJ_RS05670 - ferredoxin family protein, at 1,057,593 to 1,057,799
_RS05670
MMJJ_RS05675: MMJJ_RS05675 - RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5, at 1,057,815 to 1,058,816
_RS05675
Position (kb)
1056
1057
1058 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 1056.058 kb on - strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.070 kb on - strand, within MMJJ_RS05655 at 1056.161 kb on - strand, within MMJJ_RS05655 at 1056.161 kb on - strand, within MMJJ_RS05655 at 1056.162 kb on + strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.206 kb on - strand, within MMJJ_RS05655 at 1056.270 kb on + strand at 1056.270 kb on + strand at 1056.275 kb on + strand at 1056.275 kb on + strand at 1056.324 kb on - strand at 1056.324 kb on - strand at 1056.325 kb on + strand at 1056.325 kb on + strand at 1056.328 kb on + strand at 1056.336 kb on - strand at 1056.337 kb on + strand at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.426 kb on - strand, within MMJJ_RS05660 at 1056.461 kb on - strand, within MMJJ_RS05660 at 1056.462 kb on + strand, within MMJJ_RS05660 at 1056.462 kb on + strand, within MMJJ_RS05660 at 1056.500 kb on - strand, within MMJJ_RS05660 at 1056.512 kb on - strand, within MMJJ_RS05660 at 1056.524 kb on - strand, within MMJJ_RS05660 at 1056.524 kb on - strand, within MMJJ_RS05660 at 1056.551 kb on - strand, within MMJJ_RS05660 at 1056.552 kb on + strand, within MMJJ_RS05660 at 1056.609 kb on + strand, within MMJJ_RS05660 at 1056.609 kb on + strand, within MMJJ_RS05660 at 1056.609 kb on + strand, within MMJJ_RS05660 at 1056.614 kb on - strand, within MMJJ_RS05660 at 1056.615 kb on + strand, within MMJJ_RS05660 at 1056.615 kb on + strand, within MMJJ_RS05660 at 1056.683 kb on + strand, within MMJJ_RS05660 at 1056.683 kb on + strand, within MMJJ_RS05660 at 1056.683 kb on + strand, within MMJJ_RS05660 at 1056.722 kb on - strand, within MMJJ_RS05660 at 1056.727 kb on + strand, within MMJJ_RS05660 at 1056.761 kb on - strand, within MMJJ_RS05660 at 1056.764 kb on - strand, within MMJJ_RS05660 at 1056.789 kb on - strand, within MMJJ_RS05660 at 1056.790 kb on + strand, within MMJJ_RS05660 at 1056.831 kb on + strand at 1056.831 kb on + strand at 1056.831 kb on + strand at 1056.851 kb on - strand at 1056.886 kb on - strand at 1056.890 kb on - strand at 1056.891 kb on + strand at 1056.969 kb on - strand, within MMJJ_RS05665 at 1057.070 kb on - strand, within MMJJ_RS05665 at 1057.071 kb on + strand, within MMJJ_RS05665 at 1057.092 kb on - strand, within MMJJ_RS05665 at 1057.092 kb on - strand, within MMJJ_RS05665 at 1057.092 kb on - strand, within MMJJ_RS05665 at 1057.093 kb on + strand, within MMJJ_RS05665 at 1057.115 kb on - strand, within MMJJ_RS05665 at 1057.116 kb on + strand, within MMJJ_RS05665 at 1057.116 kb on + strand, within MMJJ_RS05665 at 1057.116 kb on + strand, within MMJJ_RS05665 at 1057.120 kb on - strand, within MMJJ_RS05665 at 1057.121 kb on + strand, within MMJJ_RS05665 at 1057.124 kb on - strand, within MMJJ_RS05665 at 1057.124 kb on - strand, within MMJJ_RS05665 at 1057.125 kb on + strand, within MMJJ_RS05665 at 1057.125 kb on + strand, within MMJJ_RS05665 at 1057.147 kb on + strand, within MMJJ_RS05665 at 1057.172 kb on - strand, within MMJJ_RS05665 at 1057.172 kb on - strand, within MMJJ_RS05665 at 1057.172 kb on - strand, within MMJJ_RS05665 at 1057.173 kb on + strand, within MMJJ_RS05665 at 1057.261 kb on - strand, within MMJJ_RS05665 at 1057.262 kb on + strand, within MMJJ_RS05665 at 1057.262 kb on + strand, within MMJJ_RS05665 at 1057.278 kb on - strand, within MMJJ_RS05665 at 1057.282 kb on - strand, within MMJJ_RS05665 at 1057.282 kb on - strand, within MMJJ_RS05665 at 1057.325 kb on - strand, within MMJJ_RS05665 at 1057.332 kb on - strand, within MMJJ_RS05665 at 1057.332 kb on - strand, within MMJJ_RS05665 at 1057.449 kb on - strand, within MMJJ_RS05665 at 1057.450 kb on + strand, within MMJJ_RS05665 at 1057.450 kb on + strand, within MMJJ_RS05665 at 1057.460 kb on + strand, within MMJJ_RS05665 at 1057.480 kb on - strand, within MMJJ_RS05665 at 1057.481 kb on + strand, within MMJJ_RS05665 at 1057.516 kb on + strand at 1057.531 kb on - strand at 1057.531 kb on - strand at 1057.531 kb on - strand at 1057.539 kb on + strand at 1057.545 kb on + strand at 1057.896 kb on - strand at 1057.897 kb on + strand at 1057.898 kb on - strand at 1057.899 kb on + strand at 1057.914 kb on - strand at 1057.915 kb on + strand at 1057.943 kb on - strand, within MMJJ_RS05675 at 1057.943 kb on - strand, within MMJJ_RS05675 at 1057.943 kb on - strand, within MMJJ_RS05675 at 1058.059 kb on - strand, within MMJJ_RS05675 at 1058.060 kb on + strand, within MMJJ_RS05675 at 1058.066 kb on - strand, within MMJJ_RS05675 at 1058.066 kb on - strand, within MMJJ_RS05675 at 1058.079 kb on - strand, within MMJJ_RS05675 at 1058.079 kb on - strand, within MMJJ_RS05675 at 1058.080 kb on + strand, within MMJJ_RS05675 at 1058.117 kb on - strand, within MMJJ_RS05675 at 1058.127 kb on - strand, within MMJJ_RS05675 at 1058.128 kb on + strand, within MMJJ_RS05675 at 1058.128 kb on + strand, within MMJJ_RS05675 at 1058.128 kb on + strand, within MMJJ_RS05675 at 1058.134 kb on - strand, within MMJJ_RS05675 at 1058.134 kb on - strand, within MMJJ_RS05675 at 1058.149 kb on + strand, within MMJJ_RS05675 at 1058.195 kb on - strand, within MMJJ_RS05675 at 1058.195 kb on - strand, within MMJJ_RS05675 at 1058.195 kb on - strand, within MMJJ_RS05675 at 1058.198 kb on + strand, within MMJJ_RS05675 at 1058.208 kb on - strand, within MMJJ_RS05675 at 1058.252 kb on - strand, within MMJJ_RS05675 at 1058.255 kb on - strand, within MMJJ_RS05675 at 1058.306 kb on - strand, within MMJJ_RS05675 at 1058.329 kb on - strand, within MMJJ_RS05675 at 1058.330 kb on + strand, within MMJJ_RS05675 at 1058.330 kb on + strand, within MMJJ_RS05675 at 1058.330 kb on + strand, within MMJJ_RS05675 at 1058.330 kb on + strand, within MMJJ_RS05675 at 1058.354 kb on - strand, within MMJJ_RS05675 at 1058.355 kb on + strand, within MMJJ_RS05675 at 1058.358 kb on - strand, within MMJJ_RS05675 at 1058.363 kb on - strand, within MMJJ_RS05675 at 1058.364 kb on + strand, within MMJJ_RS05675 at 1058.468 kb on - strand, within MMJJ_RS05675 at 1058.469 kb on + strand, within MMJJ_RS05675 at 1058.504 kb on - strand, within MMJJ_RS05675 at 1058.504 kb on - strand, within MMJJ_RS05675 at 1058.532 kb on - strand, within MMJJ_RS05675 at 1058.532 kb on - strand, within MMJJ_RS05675 at 1058.532 kb on - strand, within MMJJ_RS05675 at 1058.532 kb on - strand, within MMJJ_RS05675 at 1058.532 kb on - strand, within MMJJ_RS05675 at 1058.533 kb on + strand, within MMJJ_RS05675 at 1058.533 kb on + strand, within MMJJ_RS05675 at 1058.533 kb on + strand, within MMJJ_RS05675 at 1058.533 kb on + strand, within MMJJ_RS05675 at 1058.533 kb on + strand, within MMJJ_RS05675 at 1058.536 kb on - strand, within MMJJ_RS05675 at 1058.536 kb on - strand, within MMJJ_RS05675 at 1058.536 kb on - strand, within MMJJ_RS05675
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM remove 1,056,058 - MMJJ_RS05655 0.60 -1.1 1,056,059 + MMJJ_RS05655 0.60 +0.1 1,056,059 + MMJJ_RS05655 0.60 -0.7 1,056,059 + MMJJ_RS05655 0.60 +0.7 1,056,070 - MMJJ_RS05655 0.62 -0.7 1,056,161 - MMJJ_RS05655 0.75 -1.8 1,056,161 - MMJJ_RS05655 0.75 +0.1 1,056,162 + MMJJ_RS05655 0.75 +1.7 1,056,189 - MMJJ_RS05655 0.79 -1.5 1,056,189 - MMJJ_RS05655 0.79 -0.7 1,056,189 - MMJJ_RS05655 0.79 -0.7 1,056,189 - MMJJ_RS05655 0.79 +0.7 1,056,189 - MMJJ_RS05655 0.79 -5.0 1,056,190 + MMJJ_RS05655 0.80 -0.3 1,056,190 + MMJJ_RS05655 0.80 +0.1 1,056,190 + MMJJ_RS05655 0.80 -0.9 1,056,190 + MMJJ_RS05655 0.80 -1.7 1,056,206 - MMJJ_RS05655 0.82 -0.3 1,056,270 + -0.7 1,056,270 + -0.4 1,056,275 + -1.1 1,056,275 + +0.1 1,056,324 - -2.4 1,056,324 - -1.0 1,056,325 + +3.0 1,056,325 + -1.7 1,056,328 + +2.1 1,056,336 - +1.1 1,056,337 + -3.5 1,056,386 - MMJJ_RS05660 0.10 +1.5 1,056,386 - MMJJ_RS05660 0.10 +0.1 1,056,386 - MMJJ_RS05660 0.10 -2.9 1,056,426 - MMJJ_RS05660 0.18 -2.9 1,056,461 - MMJJ_RS05660 0.24 -0.7 1,056,462 + MMJJ_RS05660 0.24 +0.0 1,056,462 + MMJJ_RS05660 0.24 -2.0 1,056,500 - MMJJ_RS05660 0.31 +1.9 1,056,512 - MMJJ_RS05660 0.33 -1.1 1,056,524 - MMJJ_RS05660 0.35 +2.2 1,056,524 - MMJJ_RS05660 0.35 -3.1 1,056,551 - MMJJ_RS05660 0.40 +1.7 1,056,552 + MMJJ_RS05660 0.40 -2.9 1,056,609 + MMJJ_RS05660 0.50 +0.1 1,056,609 + MMJJ_RS05660 0.50 +1.2 1,056,609 + MMJJ_RS05660 0.50 +3.5 1,056,614 - MMJJ_RS05660 0.51 +2.1 1,056,615 + MMJJ_RS05660 0.51 +1.1 1,056,615 + MMJJ_RS05660 0.51 +0.1 1,056,683 + MMJJ_RS05660 0.64 +0.1 1,056,683 + MMJJ_RS05660 0.64 -2.4 1,056,683 + MMJJ_RS05660 0.64 +1.5 1,056,722 - MMJJ_RS05660 0.71 -2.9 1,056,727 + MMJJ_RS05660 0.72 -0.6 1,056,761 - MMJJ_RS05660 0.78 +0.3 1,056,764 - MMJJ_RS05660 0.78 +0.4 1,056,789 - MMJJ_RS05660 0.83 -1.0 1,056,790 + MMJJ_RS05660 0.83 -1.9 1,056,831 + -1.7 1,056,831 + -0.2 1,056,831 + +0.8 1,056,851 - -0.7 1,056,886 - -3.6 1,056,890 - +2.2 1,056,891 + -1.3 1,056,969 - MMJJ_RS05665 0.10 -1.8 1,057,070 - MMJJ_RS05665 0.25 -1.3 1,057,071 + MMJJ_RS05665 0.25 -0.3 1,057,092 - MMJJ_RS05665 0.28 +2.4 1,057,092 - MMJJ_RS05665 0.28 -2.5 1,057,092 - MMJJ_RS05665 0.28 -0.5 1,057,093 + MMJJ_RS05665 0.29 -2.9 1,057,115 - MMJJ_RS05665 0.32 +1.3 1,057,116 + MMJJ_RS05665 0.32 +0.3 1,057,116 + MMJJ_RS05665 0.32 +1.2 1,057,116 + MMJJ_RS05665 0.32 -2.9 1,057,120 - MMJJ_RS05665 0.32 -1.4 1,057,121 + MMJJ_RS05665 0.33 -2.7 1,057,124 - MMJJ_RS05665 0.33 -0.3 1,057,124 - MMJJ_RS05665 0.33 -0.9 1,057,125 + MMJJ_RS05665 0.33 +0.7 1,057,125 + MMJJ_RS05665 0.33 +1.8 1,057,147 + MMJJ_RS05665 0.36 -0.4 1,057,172 - MMJJ_RS05665 0.40 -0.3 1,057,172 - MMJJ_RS05665 0.40 +1.6 1,057,172 - MMJJ_RS05665 0.40 +0.8 1,057,173 + MMJJ_RS05665 0.40 +2.4 1,057,261 - MMJJ_RS05665 0.53 +3.4 1,057,262 + MMJJ_RS05665 0.53 -0.2 1,057,262 + MMJJ_RS05665 0.53 -2.3 1,057,278 - MMJJ_RS05665 0.56 +0.5 1,057,282 - MMJJ_RS05665 0.56 -3.7 1,057,282 - MMJJ_RS05665 0.56 -2.1 1,057,325 - MMJJ_RS05665 0.62 +0.4 1,057,332 - MMJJ_RS05665 0.63 +0.9 1,057,332 - MMJJ_RS05665 0.63 +2.2 1,057,449 - MMJJ_RS05665 0.81 -3.0 1,057,450 + MMJJ_RS05665 0.81 -0.3 1,057,450 + MMJJ_RS05665 0.81 -3.2 1,057,460 + MMJJ_RS05665 0.82 -3.0 1,057,480 - MMJJ_RS05665 0.85 -0.4 1,057,481 + MMJJ_RS05665 0.85 -0.6 1,057,516 + -0.2 1,057,531 - +5.2 1,057,531 - -2.6 1,057,531 - -0.9 1,057,539 + -0.3 1,057,545 + -1.2 1,057,896 - -1.4 1,057,897 + -0.7 1,057,898 - -2.2 1,057,899 + -0.9 1,057,914 - -0.7 1,057,915 + +1.9 1,057,943 - MMJJ_RS05675 0.13 -3.7 1,057,943 - MMJJ_RS05675 0.13 -3.4 1,057,943 - MMJJ_RS05675 0.13 -2.1 1,058,059 - MMJJ_RS05675 0.24 +0.2 1,058,060 + MMJJ_RS05675 0.24 -0.4 1,058,066 - MMJJ_RS05675 0.25 -4.4 1,058,066 - MMJJ_RS05675 0.25 -2.9 1,058,079 - MMJJ_RS05675 0.26 -1.9 1,058,079 - MMJJ_RS05675 0.26 +3.5 1,058,080 + MMJJ_RS05675 0.26 -0.7 1,058,117 - MMJJ_RS05675 0.30 -3.7 1,058,127 - MMJJ_RS05675 0.31 +0.5 1,058,128 + MMJJ_RS05675 0.31 -0.5 1,058,128 + MMJJ_RS05675 0.31 -3.8 1,058,128 + MMJJ_RS05675 0.31 +0.9 1,058,134 - MMJJ_RS05675 0.32 -1.0 1,058,134 - MMJJ_RS05675 0.32 +2.0 1,058,149 + MMJJ_RS05675 0.33 -0.7 1,058,195 - MMJJ_RS05675 0.38 -1.2 1,058,195 - MMJJ_RS05675 0.38 -1.0 1,058,195 - MMJJ_RS05675 0.38 -0.7 1,058,198 + MMJJ_RS05675 0.38 -2.7 1,058,208 - MMJJ_RS05675 0.39 +1.2 1,058,252 - MMJJ_RS05675 0.44 -2.5 1,058,255 - MMJJ_RS05675 0.44 -3.1 1,058,306 - MMJJ_RS05675 0.49 -1.5 1,058,329 - MMJJ_RS05675 0.51 -0.9 1,058,330 + MMJJ_RS05675 0.51 +0.1 1,058,330 + MMJJ_RS05675 0.51 -3.0 1,058,330 + MMJJ_RS05675 0.51 -0.9 1,058,330 + MMJJ_RS05675 0.51 -0.3 1,058,354 - MMJJ_RS05675 0.54 +0.3 1,058,355 + MMJJ_RS05675 0.54 +2.6 1,058,358 - MMJJ_RS05675 0.54 -0.7 1,058,363 - MMJJ_RS05675 0.55 +0.4 1,058,364 + MMJJ_RS05675 0.55 +3.1 1,058,468 - MMJJ_RS05675 0.65 +3.8 1,058,469 + MMJJ_RS05675 0.65 -0.9 1,058,504 - MMJJ_RS05675 0.69 -0.6 1,058,504 - MMJJ_RS05675 0.69 -3.5 1,058,532 - MMJJ_RS05675 0.72 +0.9 1,058,532 - MMJJ_RS05675 0.72 -1.2 1,058,532 - MMJJ_RS05675 0.72 +2.7 1,058,532 - MMJJ_RS05675 0.72 -0.4 1,058,532 - MMJJ_RS05675 0.72 +0.9 1,058,533 + MMJJ_RS05675 0.72 +0.9 1,058,533 + MMJJ_RS05675 0.72 +2.1 1,058,533 + MMJJ_RS05675 0.72 -0.4 1,058,533 + MMJJ_RS05675 0.72 +0.0 1,058,533 + MMJJ_RS05675 0.72 -0.4 1,058,536 - MMJJ_RS05675 0.72 +0.1 1,058,536 - MMJJ_RS05675 0.72 +1.7 1,058,536 - MMJJ_RS05675 0.72 +1.0
Or see this region's nucleotide sequence