Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03065 and MMJJ_RS03070 are separated by 194 nucleotides MMJJ_RS03070 and MMJJ_RS03075 are separated by 12 nucleotides MMJJ_RS03075 and MMJJ_RS03080 are separated by 141 nucleotides
MMJJ_RS03065: MMJJ_RS03065 - CBS domain-containing protein, at 575,768 to 576,733
_RS03065
MMJJ_RS03070: MMJJ_RS03070 - MetS family NSS transporter small subunit, at 576,928 to 577,029
_RS03070
MMJJ_RS03075: MMJJ_RS03075 - sodium-dependent transporter, at 577,042 to 578,553
_RS03075
MMJJ_RS03080: MMJJ_RS03080 - carbohydrate kinase family protein, at 578,695 to 579,603
_RS03080
Position (kb) 577
578
579 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 576.108 kb on + strand, within MMJJ_RS03065 at 576.108 kb on + strand, within MMJJ_RS03065 at 576.157 kb on + strand, within MMJJ_RS03065 at 576.160 kb on - strand, within MMJJ_RS03065 at 576.167 kb on + strand, within MMJJ_RS03065 at 576.167 kb on + strand, within MMJJ_RS03065 at 576.186 kb on + strand, within MMJJ_RS03065 at 576.221 kb on + strand, within MMJJ_RS03065 at 576.221 kb on - strand, within MMJJ_RS03065 at 576.222 kb on + strand, within MMJJ_RS03065 at 576.368 kb on - strand, within MMJJ_RS03065 at 576.368 kb on - strand, within MMJJ_RS03065 at 576.368 kb on - strand, within MMJJ_RS03065 at 576.369 kb on + strand, within MMJJ_RS03065 at 576.369 kb on + strand, within MMJJ_RS03065 at 576.369 kb on + strand, within MMJJ_RS03065 at 576.369 kb on + strand, within MMJJ_RS03065 at 576.397 kb on - strand, within MMJJ_RS03065 at 576.397 kb on - strand, within MMJJ_RS03065 at 576.422 kb on - strand, within MMJJ_RS03065 at 576.437 kb on + strand, within MMJJ_RS03065 at 576.437 kb on + strand, within MMJJ_RS03065 at 576.437 kb on + strand, within MMJJ_RS03065 at 576.466 kb on - strand, within MMJJ_RS03065 at 576.470 kb on - strand, within MMJJ_RS03065 at 576.500 kb on - strand, within MMJJ_RS03065 at 576.500 kb on - strand, within MMJJ_RS03065 at 576.503 kb on + strand, within MMJJ_RS03065 at 576.503 kb on + strand, within MMJJ_RS03065 at 576.563 kb on - strand, within MMJJ_RS03065 at 576.563 kb on - strand, within MMJJ_RS03065 at 576.564 kb on + strand, within MMJJ_RS03065 at 576.564 kb on + strand, within MMJJ_RS03065 at 576.564 kb on + strand, within MMJJ_RS03065 at 576.573 kb on + strand, within MMJJ_RS03065 at 576.596 kb on - strand, within MMJJ_RS03065 at 576.644 kb on + strand at 576.644 kb on + strand at 576.648 kb on + strand at 576.648 kb on + strand at 576.648 kb on + strand at 576.651 kb on - strand at 576.652 kb on + strand at 576.655 kb on - strand at 576.656 kb on + strand at 576.659 kb on - strand at 576.663 kb on - strand at 576.663 kb on - strand at 576.663 kb on - strand at 576.664 kb on + strand at 576.664 kb on + strand at 576.664 kb on + strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.694 kb on - strand at 576.695 kb on + strand at 576.695 kb on + strand at 576.695 kb on + strand at 576.695 kb on + strand at 576.695 kb on + strand at 576.696 kb on - strand at 576.696 kb on - strand at 576.696 kb on - strand at 576.696 kb on - strand at 576.696 kb on - strand at 576.696 kb on - strand at 576.697 kb on + strand at 576.697 kb on + strand at 576.697 kb on + strand at 576.697 kb on + strand at 576.698 kb on - strand at 576.708 kb on + strand at 576.724 kb on - strand at 576.724 kb on - strand at 576.725 kb on + strand at 576.728 kb on - strand at 576.734 kb on - strand at 576.775 kb on - strand at 576.778 kb on - strand at 576.785 kb on - strand at 576.785 kb on - strand at 576.785 kb on - strand at 576.785 kb on - strand at 576.785 kb on - strand at 576.785 kb on - strand at 576.785 kb on - strand at 576.786 kb on + strand at 576.786 kb on + strand at 576.786 kb on + strand at 576.786 kb on + strand at 576.787 kb on - strand at 576.787 kb on - strand at 576.788 kb on + strand at 576.788 kb on + strand at 576.790 kb on - strand at 576.790 kb on - strand at 576.790 kb on - strand at 576.792 kb on - strand at 576.793 kb on + strand at 576.793 kb on + strand at 576.799 kb on - strand at 576.799 kb on - strand at 576.838 kb on - strand at 576.859 kb on + strand at 576.880 kb on + strand at 576.880 kb on + strand at 576.890 kb on - strand at 576.929 kb on - strand at 576.930 kb on + strand at 576.965 kb on - strand, within MMJJ_RS03070 at 576.965 kb on - strand, within MMJJ_RS03070 at 577.000 kb on - strand, within MMJJ_RS03070 at 577.001 kb on + strand, within MMJJ_RS03070 at 577.009 kb on - strand, within MMJJ_RS03070 at 577.010 kb on + strand, within MMJJ_RS03070 at 577.032 kb on - strand at 577.035 kb on - strand at 577.036 kb on + strand at 577.036 kb on + strand at 577.043 kb on - strand at 577.119 kb on - strand at 577.124 kb on + strand at 577.144 kb on - strand at 577.144 kb on - strand at 577.204 kb on - strand, within MMJJ_RS03075 at 577.229 kb on + strand, within MMJJ_RS03075 at 577.235 kb on - strand, within MMJJ_RS03075 at 577.236 kb on + strand, within MMJJ_RS03075 at 577.236 kb on + strand, within MMJJ_RS03075 at 577.236 kb on + strand, within MMJJ_RS03075 at 577.296 kb on + strand, within MMJJ_RS03075 at 577.370 kb on + strand, within MMJJ_RS03075 at 577.403 kb on - strand, within MMJJ_RS03075 at 577.404 kb on + strand, within MMJJ_RS03075 at 577.411 kb on - strand, within MMJJ_RS03075 at 577.412 kb on + strand, within MMJJ_RS03075 at 577.427 kb on - strand, within MMJJ_RS03075 at 577.448 kb on - strand, within MMJJ_RS03075 at 577.448 kb on - strand, within MMJJ_RS03075 at 577.480 kb on + strand, within MMJJ_RS03075 at 577.583 kb on + strand, within MMJJ_RS03075 at 577.583 kb on + strand, within MMJJ_RS03075 at 577.598 kb on + strand, within MMJJ_RS03075 at 577.598 kb on + strand, within MMJJ_RS03075 at 577.598 kb on + strand, within MMJJ_RS03075 at 577.610 kb on + strand, within MMJJ_RS03075 at 577.692 kb on - strand, within MMJJ_RS03075 at 577.697 kb on + strand, within MMJJ_RS03075 at 577.802 kb on + strand, within MMJJ_RS03075 at 577.802 kb on + strand, within MMJJ_RS03075 at 577.816 kb on + strand, within MMJJ_RS03075 at 577.823 kb on - strand, within MMJJ_RS03075 at 577.823 kb on - strand, within MMJJ_RS03075 at 577.846 kb on + strand, within MMJJ_RS03075 at 577.937 kb on - strand, within MMJJ_RS03075 at 577.940 kb on - strand, within MMJJ_RS03075 at 577.941 kb on + strand, within MMJJ_RS03075 at 577.954 kb on + strand, within MMJJ_RS03075 at 577.971 kb on - strand, within MMJJ_RS03075 at 578.021 kb on + strand, within MMJJ_RS03075 at 578.052 kb on - strand, within MMJJ_RS03075 at 578.065 kb on - strand, within MMJJ_RS03075 at 578.076 kb on + strand, within MMJJ_RS03075 at 578.076 kb on + strand, within MMJJ_RS03075 at 578.080 kb on - strand, within MMJJ_RS03075 at 578.080 kb on - strand, within MMJJ_RS03075 at 578.225 kb on - strand, within MMJJ_RS03075 at 578.225 kb on - strand, within MMJJ_RS03075 at 578.231 kb on + strand, within MMJJ_RS03075 at 578.245 kb on + strand, within MMJJ_RS03075 at 578.245 kb on + strand, within MMJJ_RS03075 at 578.255 kb on - strand, within MMJJ_RS03075 at 578.258 kb on - strand, within MMJJ_RS03075 at 578.265 kb on - strand, within MMJJ_RS03075 at 578.269 kb on - strand, within MMJJ_RS03075 at 578.285 kb on + strand, within MMJJ_RS03075 at 578.285 kb on + strand, within MMJJ_RS03075 at 578.408 kb on + strand at 578.462 kb on - strand at 578.477 kb on + strand at 578.479 kb on + strand at 578.600 kb on - strand at 578.607 kb on - strand at 578.607 kb on - strand at 578.608 kb on + strand at 578.609 kb on - strand at 578.610 kb on + strand at 578.611 kb on - strand at 578.612 kb on + strand at 578.666 kb on - strand at 578.666 kb on - strand at 578.666 kb on - strand at 578.667 kb on + strand at 578.667 kb on + strand at 578.674 kb on + strand at 578.697 kb on + strand at 578.698 kb on - strand at 578.699 kb on + strand at 578.716 kb on + strand at 578.774 kb on + strand at 578.774 kb on + strand at 578.788 kb on - strand, within MMJJ_RS03080 at 578.788 kb on - strand, within MMJJ_RS03080 at 578.789 kb on + strand, within MMJJ_RS03080 at 578.789 kb on + strand, within MMJJ_RS03080 at 578.833 kb on - strand, within MMJJ_RS03080 at 578.837 kb on - strand, within MMJJ_RS03080 at 578.837 kb on - strand, within MMJJ_RS03080 at 578.902 kb on - strand, within MMJJ_RS03080 at 578.916 kb on - strand, within MMJJ_RS03080 at 578.917 kb on + strand, within MMJJ_RS03080 at 578.917 kb on + strand, within MMJJ_RS03080 at 578.936 kb on - strand, within MMJJ_RS03080 at 578.938 kb on - strand, within MMJJ_RS03080 at 578.938 kb on - strand, within MMJJ_RS03080 at 578.938 kb on - strand, within MMJJ_RS03080 at 578.938 kb on - strand, within MMJJ_RS03080 at 578.939 kb on + strand, within MMJJ_RS03080 at 578.939 kb on + strand, within MMJJ_RS03080 at 578.966 kb on + strand, within MMJJ_RS03080 at 578.971 kb on - strand, within MMJJ_RS03080 at 578.989 kb on + strand, within MMJJ_RS03080 at 579.096 kb on - strand, within MMJJ_RS03080 at 579.101 kb on - strand, within MMJJ_RS03080 at 579.101 kb on - strand, within MMJJ_RS03080 at 579.102 kb on + strand, within MMJJ_RS03080 at 579.102 kb on + strand, within MMJJ_RS03080 at 579.103 kb on - strand, within MMJJ_RS03080 at 579.109 kb on - strand, within MMJJ_RS03080 at 579.110 kb on + strand, within MMJJ_RS03080 at 579.205 kb on - strand, within MMJJ_RS03080 at 579.424 kb on - strand, within MMJJ_RS03080 at 579.424 kb on - strand, within MMJJ_RS03080 at 579.468 kb on - strand, within MMJJ_RS03080 at 579.550 kb on - strand at 579.550 kb on - strand at 579.552 kb on - strand at 579.552 kb on - strand at 579.552 kb on - strand at 579.553 kb on + strand at 579.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM remove 576,108 + MMJJ_RS03065 0.35 -3.0 576,108 + MMJJ_RS03065 0.35 +0.8 576,157 + MMJJ_RS03065 0.40 +0.0 576,160 - MMJJ_RS03065 0.41 -1.3 576,167 + MMJJ_RS03065 0.41 -3.6 576,167 + MMJJ_RS03065 0.41 -2.1 576,186 + MMJJ_RS03065 0.43 -1.7 576,221 + MMJJ_RS03065 0.47 -2.6 576,221 - MMJJ_RS03065 0.47 -1.3 576,222 + MMJJ_RS03065 0.47 -0.1 576,368 - MMJJ_RS03065 0.62 -3.3 576,368 - MMJJ_RS03065 0.62 -0.9 576,368 - MMJJ_RS03065 0.62 -2.3 576,369 + MMJJ_RS03065 0.62 -2.5 576,369 + MMJJ_RS03065 0.62 -2.1 576,369 + MMJJ_RS03065 0.62 -1.8 576,369 + MMJJ_RS03065 0.62 -2.6 576,397 - MMJJ_RS03065 0.65 -3.4 576,397 - MMJJ_RS03065 0.65 -0.6 576,422 - MMJJ_RS03065 0.68 -0.1 576,437 + MMJJ_RS03065 0.69 -1.6 576,437 + MMJJ_RS03065 0.69 -1.1 576,437 + MMJJ_RS03065 0.69 -0.9 576,466 - MMJJ_RS03065 0.72 -1.6 576,470 - MMJJ_RS03065 0.73 -0.7 576,500 - MMJJ_RS03065 0.76 -2.3 576,500 - MMJJ_RS03065 0.76 -3.1 576,503 + MMJJ_RS03065 0.76 +0.4 576,503 + MMJJ_RS03065 0.76 -2.8 576,563 - MMJJ_RS03065 0.82 -0.6 576,563 - MMJJ_RS03065 0.82 -1.6 576,564 + MMJJ_RS03065 0.82 -2.0 576,564 + MMJJ_RS03065 0.82 -3.5 576,564 + MMJJ_RS03065 0.82 -2.5 576,573 + MMJJ_RS03065 0.83 -1.8 576,596 - MMJJ_RS03065 0.86 -1.1 576,644 + +0.4 576,644 + -2.6 576,648 + -0.6 576,648 + -1.7 576,648 + -3.1 576,651 - -2.4 576,652 + -1.9 576,655 - -0.9 576,656 + -3.4 576,659 - -0.9 576,663 - -0.9 576,663 - -1.6 576,663 - +1.1 576,664 + -2.6 576,664 + +0.2 576,664 + -1.5 576,694 - -1.6 576,694 - -2.3 576,694 - -1.7 576,694 - -3.0 576,694 - -3.0 576,694 - -0.9 576,694 - +1.4 576,694 - -1.9 576,694 - -3.1 576,695 + -3.0 576,695 + -0.9 576,695 + -2.6 576,695 + -2.6 576,695 + -1.7 576,696 - -2.0 576,696 - -3.1 576,696 - -4.0 576,696 - -2.0 576,696 - +1.6 576,696 - -2.7 576,697 + -1.5 576,697 + +0.6 576,697 + +0.3 576,697 + -2.1 576,698 - -1.4 576,708 + -0.1 576,724 - -0.6 576,724 - +0.6 576,725 + -1.4 576,728 - +0.0 576,734 - +0.5 576,775 - -0.1 576,778 - -1.7 576,785 - -2.4 576,785 - +0.6 576,785 - -1.2 576,785 - -0.3 576,785 - -1.6 576,785 - -1.9 576,785 - -0.3 576,786 + -1.1 576,786 + -2.4 576,786 + -2.0 576,786 + -1.1 576,787 - -0.6 576,787 - +0.3 576,788 + -2.0 576,788 + -3.1 576,790 - -1.9 576,790 - +2.2 576,790 - +1.3 576,792 - -3.1 576,793 + -0.4 576,793 + -2.7 576,799 - -1.7 576,799 - +2.7 576,838 - -3.2 576,859 + +2.2 576,880 + -2.1 576,880 + +0.6 576,890 - +0.2 576,929 - +1.0 576,930 + -0.4 576,965 - MMJJ_RS03070 0.36 +0.6 576,965 - MMJJ_RS03070 0.36 +0.5 577,000 - MMJJ_RS03070 0.71 -0.9 577,001 + MMJJ_RS03070 0.72 -1.4 577,009 - MMJJ_RS03070 0.79 -1.6 577,010 + MMJJ_RS03070 0.80 -1.8 577,032 - +0.5 577,035 - -1.5 577,036 + +0.7 577,036 + +0.1 577,043 - +3.4 577,119 - -2.0 577,124 + -3.1 577,144 - +3.8 577,144 - -3.2 577,204 - MMJJ_RS03075 0.11 +1.3 577,229 + MMJJ_RS03075 0.12 +2.4 577,235 - MMJJ_RS03075 0.13 +1.2 577,236 + MMJJ_RS03075 0.13 -2.3 577,236 + MMJJ_RS03075 0.13 -1.9 577,236 + MMJJ_RS03075 0.13 +0.1 577,296 + MMJJ_RS03075 0.17 +1.7 577,370 + MMJJ_RS03075 0.22 +4.0 577,403 - MMJJ_RS03075 0.24 +0.1 577,404 + MMJJ_RS03075 0.24 +2.5 577,411 - MMJJ_RS03075 0.24 +2.6 577,412 + MMJJ_RS03075 0.24 -1.7 577,427 - MMJJ_RS03075 0.25 -2.5 577,448 - MMJJ_RS03075 0.27 -1.7 577,448 - MMJJ_RS03075 0.27 -0.6 577,480 + MMJJ_RS03075 0.29 +0.4 577,583 + MMJJ_RS03075 0.36 -2.8 577,583 + MMJJ_RS03075 0.36 -0.9 577,598 + MMJJ_RS03075 0.37 +1.4 577,598 + MMJJ_RS03075 0.37 +0.4 577,598 + MMJJ_RS03075 0.37 +0.6 577,610 + MMJJ_RS03075 0.38 +2.6 577,692 - MMJJ_RS03075 0.43 -2.6 577,697 + MMJJ_RS03075 0.43 +5.3 577,802 + MMJJ_RS03075 0.50 -0.4 577,802 + MMJJ_RS03075 0.50 +0.7 577,816 + MMJJ_RS03075 0.51 +4.1 577,823 - MMJJ_RS03075 0.52 +0.4 577,823 - MMJJ_RS03075 0.52 -0.4 577,846 + MMJJ_RS03075 0.53 +2.2 577,937 - MMJJ_RS03075 0.59 +1.6 577,940 - MMJJ_RS03075 0.59 -2.1 577,941 + MMJJ_RS03075 0.59 -2.6 577,954 + MMJJ_RS03075 0.60 -0.4 577,971 - MMJJ_RS03075 0.61 +0.7 578,021 + MMJJ_RS03075 0.65 -2.1 578,052 - MMJJ_RS03075 0.67 +2.6 578,065 - MMJJ_RS03075 0.68 +0.6 578,076 + MMJJ_RS03075 0.68 +1.7 578,076 + MMJJ_RS03075 0.68 -3.1 578,080 - MMJJ_RS03075 0.69 -0.9 578,080 - MMJJ_RS03075 0.69 +1.2 578,225 - MMJJ_RS03075 0.78 +1.6 578,225 - MMJJ_RS03075 0.78 -2.3 578,231 + MMJJ_RS03075 0.79 -3.7 578,245 + MMJJ_RS03075 0.80 +1.4 578,245 + MMJJ_RS03075 0.80 -1.9 578,255 - MMJJ_RS03075 0.80 -2.6 578,258 - MMJJ_RS03075 0.80 -1.4 578,265 - MMJJ_RS03075 0.81 -1.7 578,269 - MMJJ_RS03075 0.81 +2.6 578,285 + MMJJ_RS03075 0.82 -0.6 578,285 + MMJJ_RS03075 0.82 +1.7 578,408 + -2.9 578,462 - -0.1 578,477 + -2.5 578,479 + +1.2 578,600 - +0.9 578,607 - +0.6 578,607 - -3.7 578,608 + +1.1 578,609 - -3.5 578,610 + -2.1 578,611 - +0.4 578,612 + +0.6 578,666 - -2.5 578,666 - -2.7 578,666 - +4.4 578,667 + +3.2 578,667 + +1.6 578,674 + +0.6 578,697 + +0.7 578,698 - -0.3 578,699 + -0.1 578,716 + -0.6 578,774 + -0.0 578,774 + -3.8 578,788 - MMJJ_RS03080 0.10 +1.1 578,788 - MMJJ_RS03080 0.10 +2.0 578,789 + MMJJ_RS03080 0.10 -1.1 578,789 + MMJJ_RS03080 0.10 -2.6 578,833 - MMJJ_RS03080 0.15 -1.6 578,837 - MMJJ_RS03080 0.16 +0.8 578,837 - MMJJ_RS03080 0.16 -0.8 578,902 - MMJJ_RS03080 0.23 -3.8 578,916 - MMJJ_RS03080 0.24 -0.1 578,917 + MMJJ_RS03080 0.24 -0.1 578,917 + MMJJ_RS03080 0.24 -2.6 578,936 - MMJJ_RS03080 0.27 +1.4 578,938 - MMJJ_RS03080 0.27 -0.2 578,938 - MMJJ_RS03080 0.27 -0.9 578,938 - MMJJ_RS03080 0.27 -0.6 578,938 - MMJJ_RS03080 0.27 -2.6 578,939 + MMJJ_RS03080 0.27 -2.3 578,939 + MMJJ_RS03080 0.27 -2.5 578,966 + MMJJ_RS03080 0.30 -2.0 578,971 - MMJJ_RS03080 0.30 +1.4 578,989 + MMJJ_RS03080 0.32 -2.1 579,096 - MMJJ_RS03080 0.44 -2.7 579,101 - MMJJ_RS03080 0.45 -2.0 579,101 - MMJJ_RS03080 0.45 -2.9 579,102 + MMJJ_RS03080 0.45 -3.3 579,102 + MMJJ_RS03080 0.45 +0.6 579,103 - MMJJ_RS03080 0.45 -2.5 579,109 - MMJJ_RS03080 0.46 -3.6 579,110 + MMJJ_RS03080 0.46 -2.6 579,205 - MMJJ_RS03080 0.56 -1.2 579,424 - MMJJ_RS03080 0.80 -1.6 579,424 - MMJJ_RS03080 0.80 -1.4 579,468 - MMJJ_RS03080 0.85 +0.4 579,550 - -3.1 579,550 - +1.7 579,552 - -0.1 579,552 - -1.1 579,552 - -4.5 579,553 + -4.2 579,553 + -3.6
Or see this region's nucleotide sequence