Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02305 and MMJJ_RS02310 are separated by 194 nucleotides MMJJ_RS02310 and MMJJ_RS02315 are separated by 88 nucleotides MMJJ_RS02315 and MMJJ_RS02320 are separated by 119 nucleotides MMJJ_RS02320 and MMJJ_RS02325 are separated by 97 nucleotides
MMJJ_RS02305: MMJJ_RS02305 - hypothetical protein, at 435,503 to 436,078
_RS02305
MMJJ_RS02310: MMJJ_RS02310 - ABC transporter ATP-binding protein, at 436,273 to 436,977
_RS02310
MMJJ_RS02315: MMJJ_RS02315 - magnesium chelatase, at 437,066 to 437,710
_RS02315
MMJJ_RS02320: MMJJ_RS02320 - VWA domain-containing protein, at 437,830 to 438,564
_RS02320
MMJJ_RS02325: MMJJ_RS02325 - hypothetical protein, at 438,662 to 439,618
_RS02325
Position (kb)
437
438 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 436.066 kb on - strand at 436.080 kb on - strand at 436.080 kb on - strand at 436.080 kb on - strand at 436.080 kb on - strand at 436.081 kb on + strand at 436.081 kb on + strand at 436.081 kb on + strand at 436.114 kb on - strand at 436.114 kb on - strand at 436.178 kb on + strand at 436.184 kb on + strand at 436.189 kb on - strand at 436.190 kb on + strand at 436.248 kb on + strand at 436.272 kb on - strand at 436.273 kb on + strand at 436.273 kb on + strand at 436.273 kb on + strand at 436.285 kb on + strand at 436.287 kb on + strand at 436.313 kb on + strand at 436.365 kb on + strand, within MMJJ_RS02310 at 436.365 kb on + strand, within MMJJ_RS02310 at 436.365 kb on + strand, within MMJJ_RS02310 at 436.365 kb on + strand, within MMJJ_RS02310 at 436.369 kb on + strand, within MMJJ_RS02310 at 436.369 kb on + strand, within MMJJ_RS02310 at 436.428 kb on - strand, within MMJJ_RS02310 at 436.433 kb on - strand, within MMJJ_RS02310 at 436.434 kb on + strand, within MMJJ_RS02310 at 436.434 kb on + strand, within MMJJ_RS02310 at 436.474 kb on + strand, within MMJJ_RS02310 at 436.479 kb on - strand, within MMJJ_RS02310 at 436.494 kb on - strand, within MMJJ_RS02310 at 436.511 kb on - strand, within MMJJ_RS02310 at 436.557 kb on - strand, within MMJJ_RS02310 at 436.562 kb on - strand, within MMJJ_RS02310 at 436.766 kb on - strand, within MMJJ_RS02310 at 436.767 kb on + strand, within MMJJ_RS02310 at 436.785 kb on - strand, within MMJJ_RS02310 at 436.785 kb on - strand, within MMJJ_RS02310 at 436.785 kb on - strand, within MMJJ_RS02310 at 436.823 kb on - strand, within MMJJ_RS02310 at 436.863 kb on - strand, within MMJJ_RS02310 at 436.863 kb on - strand, within MMJJ_RS02310 at 436.866 kb on - strand, within MMJJ_RS02310 at 436.872 kb on - strand, within MMJJ_RS02310 at 436.873 kb on + strand, within MMJJ_RS02310 at 436.873 kb on + strand, within MMJJ_RS02310 at 436.873 kb on + strand, within MMJJ_RS02310 at 436.898 kb on + strand, within MMJJ_RS02310 at 436.919 kb on - strand at 436.942 kb on + strand at 436.942 kb on + strand at 436.942 kb on + strand at 436.944 kb on + strand at 436.944 kb on + strand at 436.944 kb on + strand at 436.979 kb on + strand at 436.988 kb on + strand at 437.028 kb on - strand at 437.054 kb on - strand at 437.055 kb on + strand at 437.082 kb on + strand at 437.087 kb on + strand at 437.153 kb on + strand, within MMJJ_RS02315 at 437.175 kb on - strand, within MMJJ_RS02315 at 437.176 kb on + strand, within MMJJ_RS02315 at 437.185 kb on - strand, within MMJJ_RS02315 at 437.185 kb on - strand, within MMJJ_RS02315 at 437.186 kb on + strand, within MMJJ_RS02315 at 437.221 kb on + strand, within MMJJ_RS02315 at 437.221 kb on + strand, within MMJJ_RS02315 at 437.227 kb on - strand, within MMJJ_RS02315 at 437.240 kb on - strand, within MMJJ_RS02315 at 437.240 kb on - strand, within MMJJ_RS02315 at 437.290 kb on + strand, within MMJJ_RS02315 at 437.330 kb on - strand, within MMJJ_RS02315 at 437.339 kb on - strand, within MMJJ_RS02315 at 437.339 kb on - strand, within MMJJ_RS02315 at 437.340 kb on + strand, within MMJJ_RS02315 at 437.422 kb on + strand, within MMJJ_RS02315 at 437.446 kb on + strand, within MMJJ_RS02315 at 437.446 kb on + strand, within MMJJ_RS02315 at 437.481 kb on - strand, within MMJJ_RS02315 at 437.490 kb on - strand, within MMJJ_RS02315 at 437.527 kb on - strand, within MMJJ_RS02315 at 437.527 kb on - strand, within MMJJ_RS02315 at 437.536 kb on - strand, within MMJJ_RS02315 at 437.537 kb on + strand, within MMJJ_RS02315 at 437.550 kb on - strand, within MMJJ_RS02315 at 437.586 kb on - strand, within MMJJ_RS02315 at 437.587 kb on + strand, within MMJJ_RS02315 at 437.587 kb on + strand, within MMJJ_RS02315 at 437.591 kb on + strand, within MMJJ_RS02315 at 437.635 kb on - strand, within MMJJ_RS02315 at 437.688 kb on - strand at 437.708 kb on - strand at 437.770 kb on + strand at 437.879 kb on - strand at 437.918 kb on + strand, within MMJJ_RS02320 at 437.919 kb on - strand, within MMJJ_RS02320 at 437.919 kb on - strand, within MMJJ_RS02320 at 437.920 kb on + strand, within MMJJ_RS02320 at 437.920 kb on + strand, within MMJJ_RS02320 at 437.920 kb on + strand, within MMJJ_RS02320 at 437.954 kb on - strand, within MMJJ_RS02320 at 437.955 kb on + strand, within MMJJ_RS02320 at 438.158 kb on - strand, within MMJJ_RS02320 at 438.169 kb on - strand, within MMJJ_RS02320 at 438.170 kb on + strand, within MMJJ_RS02320 at 438.172 kb on + strand, within MMJJ_RS02320 at 438.210 kb on + strand, within MMJJ_RS02320 at 438.222 kb on - strand, within MMJJ_RS02320 at 438.223 kb on + strand, within MMJJ_RS02320 at 438.239 kb on - strand, within MMJJ_RS02320 at 438.240 kb on + strand, within MMJJ_RS02320 at 438.301 kb on + strand, within MMJJ_RS02320 at 438.312 kb on + strand, within MMJJ_RS02320 at 438.371 kb on - strand, within MMJJ_RS02320 at 438.371 kb on - strand, within MMJJ_RS02320 at 438.410 kb on - strand, within MMJJ_RS02320 at 438.410 kb on - strand, within MMJJ_RS02320 at 438.465 kb on - strand, within MMJJ_RS02320 at 438.471 kb on + strand, within MMJJ_RS02320 at 438.471 kb on + strand, within MMJJ_RS02320 at 438.473 kb on + strand, within MMJJ_RS02320 at 438.473 kb on + strand, within MMJJ_RS02320 at 438.486 kb on - strand, within MMJJ_RS02320 at 438.507 kb on - strand at 438.507 kb on - strand at 438.508 kb on + strand at 438.508 kb on + strand at 438.508 kb on + strand at 438.508 kb on + strand at 438.558 kb on - strand at 438.563 kb on + strand at 438.563 kb on + strand at 438.566 kb on - strand at 438.566 kb on - strand at 438.567 kb on + strand at 438.614 kb on - strand at 438.627 kb on + strand at 438.631 kb on - strand at 438.645 kb on - strand at 438.690 kb on + strand at 438.692 kb on - strand at 438.692 kb on - strand at 438.693 kb on + strand at 438.695 kb on - strand at 438.695 kb on - strand at 438.696 kb on + strand at 438.696 kb on + strand at 438.696 kb on + strand at 438.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM remove 436,066 - -2.5 436,080 - +2.0 436,080 - -1.4 436,080 - +1.0 436,080 - -3.5 436,081 + -0.9 436,081 + -1.7 436,081 + +0.5 436,114 - +0.5 436,114 - +3.2 436,178 + -4.6 436,184 + +1.5 436,189 - -0.1 436,190 + +0.5 436,248 + -1.6 436,272 - -0.6 436,273 + -0.1 436,273 + -0.1 436,273 + -0.9 436,285 + +2.5 436,287 + +2.2 436,313 + +0.8 436,365 + MMJJ_RS02310 0.13 +0.6 436,365 + MMJJ_RS02310 0.13 +0.7 436,365 + MMJJ_RS02310 0.13 -2.5 436,365 + MMJJ_RS02310 0.13 +3.7 436,369 + MMJJ_RS02310 0.14 -2.5 436,369 + MMJJ_RS02310 0.14 -0.5 436,428 - MMJJ_RS02310 0.22 -0.6 436,433 - MMJJ_RS02310 0.23 -0.0 436,434 + MMJJ_RS02310 0.23 +2.1 436,434 + MMJJ_RS02310 0.23 -1.8 436,474 + MMJJ_RS02310 0.29 -2.7 436,479 - MMJJ_RS02310 0.29 -2.7 436,494 - MMJJ_RS02310 0.31 +1.4 436,511 - MMJJ_RS02310 0.34 +3.5 436,557 - MMJJ_RS02310 0.40 +1.3 436,562 - MMJJ_RS02310 0.41 +2.9 436,766 - MMJJ_RS02310 0.70 +0.0 436,767 + MMJJ_RS02310 0.70 -0.5 436,785 - MMJJ_RS02310 0.73 -0.5 436,785 - MMJJ_RS02310 0.73 -3.3 436,785 - MMJJ_RS02310 0.73 -0.7 436,823 - MMJJ_RS02310 0.78 -4.1 436,863 - MMJJ_RS02310 0.84 +0.4 436,863 - MMJJ_RS02310 0.84 +0.1 436,866 - MMJJ_RS02310 0.84 +2.6 436,872 - MMJJ_RS02310 0.85 +1.6 436,873 + MMJJ_RS02310 0.85 +2.7 436,873 + MMJJ_RS02310 0.85 -3.8 436,873 + MMJJ_RS02310 0.85 +1.9 436,898 + MMJJ_RS02310 0.89 -2.9 436,919 - -1.4 436,942 + +1.7 436,942 + -2.3 436,942 + -0.5 436,944 + +0.8 436,944 + -0.6 436,944 + +2.1 436,979 + -3.3 436,988 + -2.4 437,028 - +3.4 437,054 - +2.0 437,055 + +2.0 437,082 + -1.9 437,087 + +1.5 437,153 + MMJJ_RS02315 0.13 +0.5 437,175 - MMJJ_RS02315 0.17 +0.4 437,176 + MMJJ_RS02315 0.17 -0.0 437,185 - MMJJ_RS02315 0.18 -2.6 437,185 - MMJJ_RS02315 0.18 +0.9 437,186 + MMJJ_RS02315 0.19 +1.4 437,221 + MMJJ_RS02315 0.24 +1.8 437,221 + MMJJ_RS02315 0.24 +0.8 437,227 - MMJJ_RS02315 0.25 +0.5 437,240 - MMJJ_RS02315 0.27 +0.5 437,240 - MMJJ_RS02315 0.27 -2.6 437,290 + MMJJ_RS02315 0.35 -0.3 437,330 - MMJJ_RS02315 0.41 -0.5 437,339 - MMJJ_RS02315 0.42 +2.9 437,339 - MMJJ_RS02315 0.42 +4.2 437,340 + MMJJ_RS02315 0.42 -2.7 437,422 + MMJJ_RS02315 0.55 +3.4 437,446 + MMJJ_RS02315 0.59 +2.2 437,446 + MMJJ_RS02315 0.59 -0.1 437,481 - MMJJ_RS02315 0.64 +3.4 437,490 - MMJJ_RS02315 0.66 -3.1 437,527 - MMJJ_RS02315 0.71 +3.0 437,527 - MMJJ_RS02315 0.71 +1.4 437,536 - MMJJ_RS02315 0.73 -0.1 437,537 + MMJJ_RS02315 0.73 -0.8 437,550 - MMJJ_RS02315 0.75 +0.7 437,586 - MMJJ_RS02315 0.81 -2.7 437,587 + MMJJ_RS02315 0.81 +2.5 437,587 + MMJJ_RS02315 0.81 +2.1 437,591 + MMJJ_RS02315 0.81 +0.3 437,635 - MMJJ_RS02315 0.88 -2.2 437,688 - +1.1 437,708 - -0.5 437,770 + +1.8 437,879 - -3.3 437,918 + MMJJ_RS02320 0.12 +2.7 437,919 - MMJJ_RS02320 0.12 -1.0 437,919 - MMJJ_RS02320 0.12 -1.9 437,920 + MMJJ_RS02320 0.12 +0.3 437,920 + MMJJ_RS02320 0.12 +2.1 437,920 + MMJJ_RS02320 0.12 -1.2 437,954 - MMJJ_RS02320 0.17 +1.5 437,955 + MMJJ_RS02320 0.17 -0.9 438,158 - MMJJ_RS02320 0.45 +2.4 438,169 - MMJJ_RS02320 0.46 +2.8 438,170 + MMJJ_RS02320 0.46 +2.7 438,172 + MMJJ_RS02320 0.47 +1.0 438,210 + MMJJ_RS02320 0.52 -3.1 438,222 - MMJJ_RS02320 0.53 -0.9 438,223 + MMJJ_RS02320 0.53 +3.1 438,239 - MMJJ_RS02320 0.56 -0.9 438,240 + MMJJ_RS02320 0.56 -1.0 438,301 + MMJJ_RS02320 0.64 -1.9 438,312 + MMJJ_RS02320 0.66 -1.5 438,371 - MMJJ_RS02320 0.74 +0.7 438,371 - MMJJ_RS02320 0.74 -1.7 438,410 - MMJJ_RS02320 0.79 +3.2 438,410 - MMJJ_RS02320 0.79 +2.0 438,465 - MMJJ_RS02320 0.86 +0.1 438,471 + MMJJ_RS02320 0.87 -3.9 438,471 + MMJJ_RS02320 0.87 -2.2 438,473 + MMJJ_RS02320 0.87 -2.6 438,473 + MMJJ_RS02320 0.87 -0.5 438,486 - MMJJ_RS02320 0.89 -1.7 438,507 - -1.3 438,507 - +0.0 438,508 + -1.6 438,508 + -0.3 438,508 + +2.5 438,508 + +0.6 438,558 - -1.5 438,563 + +3.6 438,563 + -2.9 438,566 - -0.4 438,566 - -2.1 438,567 + +1.1 438,614 - -1.9 438,627 + +2.2 438,631 - +3.1 438,645 - -2.9 438,690 + -0.2 438,692 - -0.1 438,692 - +2.8 438,693 + +1.7 438,695 - +1.9 438,695 - +0.6 438,696 + -1.4 438,696 + -2.2 438,696 + +1.1 438,697 - -2.0
Or see this region's nucleotide sequence