Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09215 and MMJJ_RS09220 are separated by 296 nucleotides MMJJ_RS09220 and MMJJ_RS09225 are separated by 231 nucleotides MMJJ_RS09225 and MMJJ_RS09230 are separated by 156 nucleotides MMJJ_RS09230 and MMJJ_RS09235 are separated by 72 nucleotides MMJJ_RS09235 and MMJJ_RS09240 are separated by 133 nucleotides
MMJJ_RS09215: MMJJ_RS09215 - right-handed parallel beta-helix repeat-containing protein, at 1,709,738 to 1,710,487
_RS09215
MMJJ_RS09220: MMJJ_RS09220 - cupredoxin family copper-binding protein, at 1,710,784 to 1,711,140
_RS09220
MMJJ_RS09225: MMJJ_RS09225 - hypothetical protein, at 1,711,372 to 1,711,635
_RS09225
MMJJ_RS09230: MMJJ_RS09230 - hypothetical protein, at 1,711,792 to 1,712,223
_RS09230
MMJJ_RS09235: MMJJ_RS09235 - helix-turn-helix transcriptional regulator, at 1,712,296 to 1,712,496
_RS09235
MMJJ_RS09240: MMJJ_RS09240 - hypothetical protein, at 1,712,630 to 1,713,151
_RS09240
Position (kb)
1711
1712 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1710.375 kb on + strand, within MMJJ_RS09215 at 1710.379 kb on + strand, within MMJJ_RS09215 at 1710.385 kb on - strand, within MMJJ_RS09215 at 1710.385 kb on - strand, within MMJJ_RS09215 at 1710.385 kb on - strand, within MMJJ_RS09215 at 1710.545 kb on + strand at 1710.682 kb on - strand at 1710.689 kb on - strand at 1710.707 kb on - strand at 1710.708 kb on + strand at 1710.736 kb on + strand at 1710.738 kb on + strand at 1710.758 kb on - strand at 1710.758 kb on - strand at 1710.761 kb on - strand at 1710.761 kb on - strand at 1710.762 kb on + strand at 1710.788 kb on + strand at 1710.820 kb on + strand, within MMJJ_RS09220 at 1710.867 kb on - strand, within MMJJ_RS09220 at 1710.867 kb on - strand, within MMJJ_RS09220 at 1710.868 kb on + strand, within MMJJ_RS09220 at 1710.902 kb on - strand, within MMJJ_RS09220 at 1710.902 kb on - strand, within MMJJ_RS09220 at 1710.925 kb on + strand, within MMJJ_RS09220 at 1710.987 kb on + strand, within MMJJ_RS09220 at 1710.987 kb on + strand, within MMJJ_RS09220 at 1710.994 kb on + strand, within MMJJ_RS09220 at 1710.994 kb on + strand, within MMJJ_RS09220 at 1710.999 kb on + strand, within MMJJ_RS09220 at 1710.999 kb on + strand, within MMJJ_RS09220 at 1711.047 kb on + strand, within MMJJ_RS09220 at 1711.060 kb on + strand, within MMJJ_RS09220 at 1711.060 kb on + strand, within MMJJ_RS09220 at 1711.073 kb on - strand, within MMJJ_RS09220 at 1711.081 kb on - strand, within MMJJ_RS09220 at 1711.081 kb on - strand, within MMJJ_RS09220 at 1711.084 kb on + strand, within MMJJ_RS09220 at 1711.084 kb on + strand, within MMJJ_RS09220 at 1711.084 kb on + strand, within MMJJ_RS09220 at 1711.084 kb on + strand, within MMJJ_RS09220 at 1711.084 kb on + strand, within MMJJ_RS09220 at 1711.087 kb on - strand, within MMJJ_RS09220 at 1711.087 kb on - strand, within MMJJ_RS09220 at 1711.088 kb on + strand, within MMJJ_RS09220 at 1711.088 kb on + strand, within MMJJ_RS09220 at 1711.090 kb on - strand, within MMJJ_RS09220 at 1711.090 kb on - strand, within MMJJ_RS09220 at 1711.091 kb on + strand, within MMJJ_RS09220 at 1711.095 kb on - strand at 1711.095 kb on - strand, within MMJJ_RS09220 at 1711.095 kb on - strand, within MMJJ_RS09220 at 1711.096 kb on + strand, within MMJJ_RS09220 at 1711.109 kb on + strand at 1711.111 kb on + strand at 1711.168 kb on + strand at 1711.193 kb on + strand at 1711.196 kb on - strand at 1711.220 kb on - strand at 1711.221 kb on + strand at 1711.251 kb on + strand at 1711.297 kb on + strand at 1711.297 kb on + strand at 1711.321 kb on - strand at 1711.344 kb on - strand at 1711.345 kb on + strand at 1711.352 kb on + strand at 1711.352 kb on + strand at 1711.357 kb on + strand at 1711.372 kb on + strand at 1711.486 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.488 kb on - strand, within MMJJ_RS09225 at 1711.489 kb on + strand, within MMJJ_RS09225 at 1711.489 kb on + strand, within MMJJ_RS09225 at 1711.497 kb on + strand, within MMJJ_RS09225 at 1711.521 kb on + strand, within MMJJ_RS09225 at 1711.521 kb on + strand, within MMJJ_RS09225 at 1711.521 kb on + strand, within MMJJ_RS09225 at 1711.521 kb on + strand, within MMJJ_RS09225 at 1711.588 kb on - strand, within MMJJ_RS09225 at 1711.589 kb on + strand, within MMJJ_RS09225 at 1711.634 kb on + strand at 1711.634 kb on + strand at 1711.663 kb on - strand at 1711.669 kb on + strand at 1711.679 kb on - strand at 1711.679 kb on - strand at 1711.680 kb on + strand at 1711.701 kb on - strand at 1711.701 kb on - strand at 1711.702 kb on + strand at 1711.706 kb on + strand at 1711.778 kb on - strand at 1711.789 kb on - strand at 1711.790 kb on + strand at 1711.790 kb on + strand at 1711.875 kb on - strand, within MMJJ_RS09230 at 1711.876 kb on + strand, within MMJJ_RS09230 at 1711.876 kb on + strand, within MMJJ_RS09230 at 1711.945 kb on - strand, within MMJJ_RS09230 at 1711.985 kb on - strand, within MMJJ_RS09230 at 1712.028 kb on - strand, within MMJJ_RS09230 at 1712.042 kb on - strand, within MMJJ_RS09230 at 1712.094 kb on + strand, within MMJJ_RS09230 at 1712.094 kb on + strand, within MMJJ_RS09230 at 1712.229 kb on - strand at 1712.230 kb on + strand at 1712.259 kb on - strand at 1712.260 kb on + strand at 1712.260 kb on + strand at 1712.260 kb on + strand at 1712.329 kb on - strand, within MMJJ_RS09235 at 1712.408 kb on + strand, within MMJJ_RS09235 at 1712.409 kb on - strand, within MMJJ_RS09235 at 1712.471 kb on - strand, within MMJJ_RS09235 at 1712.472 kb on + strand, within MMJJ_RS09235 at 1712.536 kb on - strand at 1712.537 kb on + strand at 1712.623 kb on + strand at 1712.623 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,710,375 + MMJJ_RS09215 0.85 -0.9 1,710,379 + MMJJ_RS09215 0.85 +2.8 1,710,385 - MMJJ_RS09215 0.86 -2.1 1,710,385 - MMJJ_RS09215 0.86 -2.2 1,710,385 - MMJJ_RS09215 0.86 +1.7 1,710,545 + -2.7 1,710,682 - -2.2 1,710,689 - +1.0 1,710,707 - -0.7 1,710,708 + +0.6 1,710,736 + -0.6 1,710,738 + +2.3 1,710,758 - -0.8 1,710,758 - +0.3 1,710,761 - +0.4 1,710,761 - +0.5 1,710,762 + +2.5 1,710,788 + -1.3 1,710,820 + MMJJ_RS09220 0.10 +2.4 1,710,867 - MMJJ_RS09220 0.23 -1.1 1,710,867 - MMJJ_RS09220 0.23 +0.0 1,710,868 + MMJJ_RS09220 0.24 -1.5 1,710,902 - MMJJ_RS09220 0.33 +0.9 1,710,902 - MMJJ_RS09220 0.33 +2.4 1,710,925 + MMJJ_RS09220 0.39 -1.3 1,710,987 + MMJJ_RS09220 0.57 -0.2 1,710,987 + MMJJ_RS09220 0.57 +1.9 1,710,994 + MMJJ_RS09220 0.59 +1.2 1,710,994 + MMJJ_RS09220 0.59 +0.7 1,710,999 + MMJJ_RS09220 0.60 +1.0 1,710,999 + MMJJ_RS09220 0.60 +2.4 1,711,047 + MMJJ_RS09220 0.74 +2.6 1,711,060 + MMJJ_RS09220 0.77 -2.2 1,711,060 + MMJJ_RS09220 0.77 +4.5 1,711,073 - MMJJ_RS09220 0.81 +0.3 1,711,081 - MMJJ_RS09220 0.83 +0.0 1,711,081 - MMJJ_RS09220 0.83 -1.9 1,711,084 + MMJJ_RS09220 0.84 -0.4 1,711,084 + MMJJ_RS09220 0.84 +1.5 1,711,084 + MMJJ_RS09220 0.84 +2.1 1,711,084 + MMJJ_RS09220 0.84 +5.3 1,711,084 + MMJJ_RS09220 0.84 +1.2 1,711,087 - MMJJ_RS09220 0.85 -1.0 1,711,087 - MMJJ_RS09220 0.85 -1.6 1,711,088 + MMJJ_RS09220 0.85 -0.8 1,711,088 + MMJJ_RS09220 0.85 -1.3 1,711,090 - MMJJ_RS09220 0.86 -2.9 1,711,090 - MMJJ_RS09220 0.86 +0.4 1,711,091 + MMJJ_RS09220 0.86 -1.9 1,711,095 - -0.4 1,711,095 - MMJJ_RS09220 0.87 -1.8 1,711,095 - MMJJ_RS09220 0.87 +1.4 1,711,096 + MMJJ_RS09220 0.87 +2.6 1,711,109 + -1.5 1,711,111 + +1.2 1,711,168 + -1.1 1,711,193 + -2.6 1,711,196 - -0.1 1,711,220 - +0.4 1,711,221 + -0.8 1,711,251 + -0.6 1,711,297 + +2.1 1,711,297 + +0.2 1,711,321 - -0.6 1,711,344 - +1.2 1,711,345 + +2.7 1,711,352 + +1.6 1,711,352 + -1.6 1,711,357 + -1.1 1,711,372 + +1.6 1,711,486 - MMJJ_RS09225 0.43 +2.3 1,711,488 - MMJJ_RS09225 0.44 +1.6 1,711,488 - MMJJ_RS09225 0.44 +1.0 1,711,488 - MMJJ_RS09225 0.44 +2.7 1,711,488 - MMJJ_RS09225 0.44 -2.0 1,711,488 - MMJJ_RS09225 0.44 -1.1 1,711,488 - MMJJ_RS09225 0.44 +0.6 1,711,488 - MMJJ_RS09225 0.44 +3.7 1,711,488 - MMJJ_RS09225 0.44 +0.6 1,711,489 + MMJJ_RS09225 0.44 +0.3 1,711,489 + MMJJ_RS09225 0.44 -0.0 1,711,497 + MMJJ_RS09225 0.47 +3.4 1,711,521 + MMJJ_RS09225 0.56 -2.0 1,711,521 + MMJJ_RS09225 0.56 -0.6 1,711,521 + MMJJ_RS09225 0.56 -0.7 1,711,521 + MMJJ_RS09225 0.56 +3.6 1,711,588 - MMJJ_RS09225 0.82 +3.4 1,711,589 + MMJJ_RS09225 0.82 +0.2 1,711,634 + +0.9 1,711,634 + +4.8 1,711,663 - -1.4 1,711,669 + -2.2 1,711,679 - -2.6 1,711,679 - +0.8 1,711,680 + -1.8 1,711,701 - -0.7 1,711,701 - +1.3 1,711,702 + +0.2 1,711,706 + +2.2 1,711,778 - +1.4 1,711,789 - +2.0 1,711,790 + -0.4 1,711,790 + +1.4 1,711,875 - MMJJ_RS09230 0.19 +1.5 1,711,876 + MMJJ_RS09230 0.19 +2.6 1,711,876 + MMJJ_RS09230 0.19 +1.5 1,711,945 - MMJJ_RS09230 0.35 -0.5 1,711,985 - MMJJ_RS09230 0.45 -1.2 1,712,028 - MMJJ_RS09230 0.55 +2.6 1,712,042 - MMJJ_RS09230 0.58 +1.3 1,712,094 + MMJJ_RS09230 0.70 -2.8 1,712,094 + MMJJ_RS09230 0.70 +1.6 1,712,229 - -0.4 1,712,230 + +0.2 1,712,259 - +1.7 1,712,260 + +2.5 1,712,260 + +0.8 1,712,260 + -0.9 1,712,329 - MMJJ_RS09235 0.16 +1.5 1,712,408 + MMJJ_RS09235 0.56 +3.3 1,712,409 - MMJJ_RS09235 0.56 -2.8 1,712,471 - MMJJ_RS09235 0.87 +1.2 1,712,472 + MMJJ_RS09235 0.88 -0.2 1,712,536 - +0.9 1,712,537 + -1.0 1,712,623 + -0.9 1,712,623 + -2.7
Or see this region's nucleotide sequence