Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09165 and MMJJ_RS09170 overlap by 1 nucleotides MMJJ_RS09170 and MMJJ_RS09175 overlap by 8 nucleotides MMJJ_RS09175 and MMJJ_RS09180 overlap by 20 nucleotides MMJJ_RS09180 and MMJJ_RS09185 are separated by 10 nucleotides
MMJJ_RS09165: MMJJ_RS09165 - indole-3-glycerol phosphate synthase TrpC, at 1,701,173 to 1,701,862
_RS09165
MMJJ_RS09170: MMJJ_RS09170 - anthranilate phosphoribosyltransferase, at 1,701,862 to 1,702,827
_RS09170
MMJJ_RS09175: MMJJ_RS09175 - anthranilate synthase component I, at 1,702,820 to 1,704,139
_RS09175
MMJJ_RS09180: MMJJ_RS09180 - aminodeoxychorismate/anthranilate synthase component II, at 1,704,120 to 1,704,716
_RS09180
MMJJ_RS09185: MMJJ_RS09185 - phosphoribosylanthranilate isomerase, at 1,704,727 to 1,705,362
_RS09185
Position (kb) 1702
1703
1704
1705 Strain fitness (log2 ratio) -2
-1
0
1 at 1701.961 kb on - strand at 1702.723 kb on + strand, within MMJJ_RS09170 at 1702.839 kb on - strand at 1703.893 kb on - strand, within MMJJ_RS09175 at 1703.986 kb on - strand, within MMJJ_RS09175 at 1704.492 kb on - strand, within MMJJ_RS09180
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,701,961 - +1.2 1,702,723 + MMJJ_RS09170 0.89 -0.4 1,702,839 - +0.2 1,703,893 - MMJJ_RS09175 0.81 -1.1 1,703,986 - MMJJ_RS09175 0.88 -2.4 1,704,492 - MMJJ_RS09180 0.62 -0.8
Or see this region's nucleotide sequence