Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09085 and MMJJ_RS09090 are separated by 111 nucleotides MMJJ_RS09090 and MMJJ_RS09095 are separated by 83 nucleotides MMJJ_RS09095 and MMJJ_RS09100 overlap by 8 nucleotides
MMJJ_RS09085: MMJJ_RS09085 - minichromosome maintenance protein MCM, at 1,678,709 to 1,680,841
_RS09085
MMJJ_RS09090: MMJJ_RS09090 - TetR/AcrR family transcriptional regulator, at 1,680,953 to 1,681,555
_RS09090
MMJJ_RS09095: MMJJ_RS09095 - MMPL family transporter, at 1,681,639 to 1,682,805
_RS09095
MMJJ_RS09100: MMJJ_RS09100 - COG1361 S-layer family protein, at 1,682,798 to 1,684,369
_RS09100
Position (kb)
1681
1682
1683 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1680.667 kb on - strand at 1680.691 kb on + strand at 1680.825 kb on + strand at 1680.827 kb on - strand at 1680.829 kb on - strand at 1680.856 kb on - strand at 1680.857 kb on + strand at 1680.902 kb on + strand at 1680.903 kb on - strand at 1680.907 kb on - strand at 1680.908 kb on + strand at 1680.922 kb on + strand at 1680.922 kb on + strand at 1680.926 kb on - strand at 1680.927 kb on + strand at 1680.927 kb on + strand at 1680.927 kb on + strand at 1680.927 kb on + strand at 1680.930 kb on - strand at 1680.930 kb on - strand at 1680.930 kb on - strand at 1680.931 kb on + strand at 1680.940 kb on - strand at 1680.941 kb on + strand at 1680.945 kb on + strand at 1680.945 kb on + strand at 1680.945 kb on + strand at 1680.959 kb on + strand at 1681.026 kb on - strand, within MMJJ_RS09090 at 1681.052 kb on + strand, within MMJJ_RS09090 at 1681.052 kb on + strand, within MMJJ_RS09090 at 1681.087 kb on - strand, within MMJJ_RS09090 at 1681.236 kb on + strand, within MMJJ_RS09090 at 1681.253 kb on + strand, within MMJJ_RS09090 at 1681.292 kb on + strand, within MMJJ_RS09090 at 1681.292 kb on + strand, within MMJJ_RS09090 at 1681.296 kb on - strand, within MMJJ_RS09090 at 1681.297 kb on + strand, within MMJJ_RS09090 at 1681.297 kb on + strand, within MMJJ_RS09090 at 1681.297 kb on + strand, within MMJJ_RS09090 at 1681.297 kb on + strand, within MMJJ_RS09090 at 1681.324 kb on - strand, within MMJJ_RS09090 at 1681.325 kb on + strand, within MMJJ_RS09090 at 1681.325 kb on + strand, within MMJJ_RS09090 at 1681.334 kb on + strand, within MMJJ_RS09090 at 1681.356 kb on + strand, within MMJJ_RS09090 at 1681.356 kb on + strand, within MMJJ_RS09090 at 1681.377 kb on - strand, within MMJJ_RS09090 at 1681.502 kb on - strand at 1681.502 kb on - strand at 1681.502 kb on - strand at 1681.502 kb on - strand at 1681.502 kb on - strand at 1681.503 kb on + strand at 1681.516 kb on - strand at 1681.516 kb on - strand at 1681.516 kb on - strand at 1681.517 kb on + strand at 1681.578 kb on - strand at 1681.602 kb on - strand at 1681.603 kb on + strand at 1681.627 kb on + strand at 1681.695 kb on + strand at 1681.752 kb on - strand at 1681.854 kb on + strand, within MMJJ_RS09095 at 1681.871 kb on - strand, within MMJJ_RS09095 at 1681.925 kb on - strand, within MMJJ_RS09095 at 1682.094 kb on + strand, within MMJJ_RS09095 at 1682.120 kb on - strand, within MMJJ_RS09095 at 1682.121 kb on + strand, within MMJJ_RS09095 at 1682.125 kb on - strand, within MMJJ_RS09095 at 1682.319 kb on - strand, within MMJJ_RS09095 at 1682.320 kb on + strand, within MMJJ_RS09095 at 1682.335 kb on - strand, within MMJJ_RS09095 at 1682.336 kb on + strand, within MMJJ_RS09095 at 1682.346 kb on - strand, within MMJJ_RS09095 at 1682.351 kb on - strand, within MMJJ_RS09095 at 1682.352 kb on + strand, within MMJJ_RS09095 at 1682.372 kb on - strand, within MMJJ_RS09095 at 1682.422 kb on + strand, within MMJJ_RS09095 at 1682.510 kb on + strand, within MMJJ_RS09095 at 1682.552 kb on - strand, within MMJJ_RS09095 at 1682.565 kb on + strand, within MMJJ_RS09095 at 1682.565 kb on + strand, within MMJJ_RS09095 at 1682.565 kb on + strand, within MMJJ_RS09095 at 1682.568 kb on + strand, within MMJJ_RS09095 at 1682.571 kb on - strand, within MMJJ_RS09095 at 1682.595 kb on - strand, within MMJJ_RS09095 at 1682.595 kb on - strand, within MMJJ_RS09095 at 1682.596 kb on + strand, within MMJJ_RS09095 at 1682.674 kb on + strand, within MMJJ_RS09095 at 1682.682 kb on - strand, within MMJJ_RS09095 at 1682.694 kb on - strand at 1682.778 kb on + strand at 1682.823 kb on - strand at 1682.888 kb on - strand at 1682.890 kb on - strand at 1682.890 kb on - strand at 1682.891 kb on + strand at 1682.905 kb on + strand at 1682.928 kb on - strand at 1682.967 kb on - strand, within MMJJ_RS09100 at 1682.968 kb on + strand, within MMJJ_RS09100 at 1682.980 kb on + strand, within MMJJ_RS09100 at 1683.024 kb on - strand, within MMJJ_RS09100 at 1683.086 kb on - strand, within MMJJ_RS09100 at 1683.100 kb on - strand, within MMJJ_RS09100 at 1683.128 kb on - strand, within MMJJ_RS09100 at 1683.167 kb on - strand, within MMJJ_RS09100 at 1683.168 kb on + strand, within MMJJ_RS09100 at 1683.190 kb on - strand, within MMJJ_RS09100 at 1683.204 kb on + strand, within MMJJ_RS09100 at 1683.215 kb on - strand, within MMJJ_RS09100 at 1683.218 kb on - strand, within MMJJ_RS09100 at 1683.218 kb on - strand, within MMJJ_RS09100 at 1683.220 kb on - strand, within MMJJ_RS09100 at 1683.228 kb on - strand, within MMJJ_RS09100 at 1683.369 kb on - strand, within MMJJ_RS09100 at 1683.369 kb on - strand, within MMJJ_RS09100 at 1683.372 kb on - strand, within MMJJ_RS09100 at 1683.398 kb on - strand, within MMJJ_RS09100 at 1683.479 kb on - strand, within MMJJ_RS09100 at 1683.480 kb on + strand, within MMJJ_RS09100 at 1683.483 kb on - strand, within MMJJ_RS09100 at 1683.498 kb on - strand, within MMJJ_RS09100 at 1683.537 kb on - strand, within MMJJ_RS09100 at 1683.541 kb on + strand, within MMJJ_RS09100 at 1683.547 kb on + strand, within MMJJ_RS09100 at 1683.604 kb on - strand, within MMJJ_RS09100 at 1683.707 kb on - strand, within MMJJ_RS09100 at 1683.760 kb on + strand, within MMJJ_RS09100 at 1683.791 kb on - strand, within MMJJ_RS09100
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,680,667 - -1.9 1,680,691 + +0.2 1,680,825 + -2.5 1,680,827 - -3.7 1,680,829 - -2.8 1,680,856 - +0.6 1,680,857 + +3.0 1,680,902 + +0.9 1,680,903 - -1.5 1,680,907 - +1.5 1,680,908 + -1.8 1,680,922 + +2.7 1,680,922 + -1.6 1,680,926 - +1.5 1,680,927 + -0.4 1,680,927 + +1.2 1,680,927 + -2.2 1,680,927 + +0.6 1,680,930 - -2.2 1,680,930 - +4.1 1,680,930 - -2.1 1,680,931 + +1.1 1,680,940 - -0.6 1,680,941 + +1.0 1,680,945 + -1.4 1,680,945 + -3.3 1,680,945 + -0.0 1,680,959 + +1.9 1,681,026 - MMJJ_RS09090 0.12 -1.6 1,681,052 + MMJJ_RS09090 0.16 -0.8 1,681,052 + MMJJ_RS09090 0.16 +0.9 1,681,087 - MMJJ_RS09090 0.22 -2.4 1,681,236 + MMJJ_RS09090 0.47 +0.4 1,681,253 + MMJJ_RS09090 0.50 +1.8 1,681,292 + MMJJ_RS09090 0.56 -3.6 1,681,292 + MMJJ_RS09090 0.56 -0.5 1,681,296 - MMJJ_RS09090 0.57 +2.7 1,681,297 + MMJJ_RS09090 0.57 -1.1 1,681,297 + MMJJ_RS09090 0.57 -0.1 1,681,297 + MMJJ_RS09090 0.57 -0.2 1,681,297 + MMJJ_RS09090 0.57 -0.9 1,681,324 - MMJJ_RS09090 0.62 -2.8 1,681,325 + MMJJ_RS09090 0.62 -2.4 1,681,325 + MMJJ_RS09090 0.62 -2.3 1,681,334 + MMJJ_RS09090 0.63 -0.1 1,681,356 + MMJJ_RS09090 0.67 +0.2 1,681,356 + MMJJ_RS09090 0.67 -0.5 1,681,377 - MMJJ_RS09090 0.70 -0.4 1,681,502 - +1.2 1,681,502 - -3.2 1,681,502 - +0.4 1,681,502 - -1.2 1,681,502 - -3.8 1,681,503 + -0.4 1,681,516 - +0.5 1,681,516 - -1.0 1,681,516 - -1.1 1,681,517 + +0.6 1,681,578 - +1.9 1,681,602 - -1.7 1,681,603 + +2.6 1,681,627 + +1.1 1,681,695 + -0.5 1,681,752 - -1.8 1,681,854 + MMJJ_RS09095 0.18 -1.4 1,681,871 - MMJJ_RS09095 0.20 +1.2 1,681,925 - MMJJ_RS09095 0.25 -2.1 1,682,094 + MMJJ_RS09095 0.39 -2.5 1,682,120 - MMJJ_RS09095 0.41 -3.1 1,682,121 + MMJJ_RS09095 0.41 -2.2 1,682,125 - MMJJ_RS09095 0.42 -1.1 1,682,319 - MMJJ_RS09095 0.58 -0.8 1,682,320 + MMJJ_RS09095 0.58 -2.8 1,682,335 - MMJJ_RS09095 0.60 -0.8 1,682,336 + MMJJ_RS09095 0.60 -3.4 1,682,346 - MMJJ_RS09095 0.61 -1.9 1,682,351 - MMJJ_RS09095 0.61 -2.9 1,682,352 + MMJJ_RS09095 0.61 -1.4 1,682,372 - MMJJ_RS09095 0.63 -2.7 1,682,422 + MMJJ_RS09095 0.67 -1.6 1,682,510 + MMJJ_RS09095 0.75 -1.6 1,682,552 - MMJJ_RS09095 0.78 -1.4 1,682,565 + MMJJ_RS09095 0.79 -1.7 1,682,565 + MMJJ_RS09095 0.79 -0.9 1,682,565 + MMJJ_RS09095 0.79 -3.3 1,682,568 + MMJJ_RS09095 0.80 -1.1 1,682,571 - MMJJ_RS09095 0.80 -3.5 1,682,595 - MMJJ_RS09095 0.82 -0.8 1,682,595 - MMJJ_RS09095 0.82 -3.4 1,682,596 + MMJJ_RS09095 0.82 -1.6 1,682,674 + MMJJ_RS09095 0.89 -2.6 1,682,682 - MMJJ_RS09095 0.89 -2.9 1,682,694 - -2.2 1,682,778 + -0.8 1,682,823 - -0.8 1,682,888 - -0.8 1,682,890 - -0.4 1,682,890 - -0.4 1,682,891 + -0.7 1,682,905 + -2.5 1,682,928 - -1.6 1,682,967 - MMJJ_RS09100 0.11 +0.2 1,682,968 + MMJJ_RS09100 0.11 -1.9 1,682,980 + MMJJ_RS09100 0.12 -0.9 1,683,024 - MMJJ_RS09100 0.14 -2.5 1,683,086 - MMJJ_RS09100 0.18 -1.1 1,683,100 - MMJJ_RS09100 0.19 -0.4 1,683,128 - MMJJ_RS09100 0.21 -1.9 1,683,167 - MMJJ_RS09100 0.23 +0.6 1,683,168 + MMJJ_RS09100 0.24 -2.1 1,683,190 - MMJJ_RS09100 0.25 +1.7 1,683,204 + MMJJ_RS09100 0.26 -1.4 1,683,215 - MMJJ_RS09100 0.27 -0.9 1,683,218 - MMJJ_RS09100 0.27 +0.2 1,683,218 - MMJJ_RS09100 0.27 -1.0 1,683,220 - MMJJ_RS09100 0.27 +1.7 1,683,228 - MMJJ_RS09100 0.27 -2.9 1,683,369 - MMJJ_RS09100 0.36 -1.9 1,683,369 - MMJJ_RS09100 0.36 -1.0 1,683,372 - MMJJ_RS09100 0.37 -2.2 1,683,398 - MMJJ_RS09100 0.38 -2.2 1,683,479 - MMJJ_RS09100 0.43 -1.8 1,683,480 + MMJJ_RS09100 0.43 -3.3 1,683,483 - MMJJ_RS09100 0.44 -2.4 1,683,498 - MMJJ_RS09100 0.45 -1.8 1,683,537 - MMJJ_RS09100 0.47 +0.2 1,683,541 + MMJJ_RS09100 0.47 -1.1 1,683,547 + MMJJ_RS09100 0.48 +2.8 1,683,604 - MMJJ_RS09100 0.51 -0.2 1,683,707 - MMJJ_RS09100 0.58 -1.4 1,683,760 + MMJJ_RS09100 0.61 -1.6 1,683,791 - MMJJ_RS09100 0.63 -1.8
Or see this region's nucleotide sequence