Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09010 and MMJJ_RS09015 are separated by 77 nucleotides MMJJ_RS09015 and MMJJ_RS09020 are separated by 36 nucleotides MMJJ_RS09020 and MMJJ_RS09025 are separated by 71 nucleotides MMJJ_RS09025 and MMJJ_RS09030 are separated by 21 nucleotides
MMJJ_RS09010: MMJJ_RS09010 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,662,823 to 1,663,302
_RS09010
MMJJ_RS09015: MMJJ_RS09015 - SPFH domain-containing protein, at 1,663,380 to 1,664,186
_RS09015
MMJJ_RS09020: MMJJ_RS09020 - hypothetical protein, at 1,664,223 to 1,664,636
_RS09020
MMJJ_RS09025: MMJJ_RS09025 - dTMP kinase, at 1,664,708 to 1,665,307
_RS09025
MMJJ_RS09030: MMJJ_RS09030 - hypothetical protein, at 1,665,329 to 1,668,259
_RS09030
Position (kb)
1664
1665 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1663.235 kb on - strand, within MMJJ_RS09010 at 1663.242 kb on - strand, within MMJJ_RS09010 at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.256 kb on + strand at 1663.256 kb on + strand at 1663.256 kb on + strand at 1663.259 kb on - strand at 1663.259 kb on - strand at 1663.271 kb on + strand at 1663.294 kb on - strand at 1663.294 kb on - strand at 1663.294 kb on - strand at 1663.302 kb on - strand at 1663.304 kb on - strand at 1663.329 kb on + strand at 1663.378 kb on + strand at 1663.464 kb on - strand, within MMJJ_RS09015 at 1663.683 kb on + strand, within MMJJ_RS09015 at 1663.767 kb on - strand, within MMJJ_RS09015 at 1663.819 kb on + strand, within MMJJ_RS09015 at 1663.830 kb on - strand, within MMJJ_RS09015 at 1663.860 kb on - strand, within MMJJ_RS09015 at 1663.862 kb on - strand, within MMJJ_RS09015 at 1663.863 kb on + strand, within MMJJ_RS09015 at 1663.906 kb on + strand, within MMJJ_RS09015 at 1663.925 kb on + strand, within MMJJ_RS09015 at 1663.964 kb on - strand, within MMJJ_RS09015 at 1663.993 kb on + strand, within MMJJ_RS09015 at 1663.998 kb on + strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.022 kb on - strand, within MMJJ_RS09015 at 1664.023 kb on + strand, within MMJJ_RS09015 at 1664.040 kb on - strand, within MMJJ_RS09015 at 1664.040 kb on - strand, within MMJJ_RS09015 at 1664.049 kb on + strand, within MMJJ_RS09015 at 1664.091 kb on - strand, within MMJJ_RS09015 at 1664.093 kb on - strand, within MMJJ_RS09015 at 1664.093 kb on - strand, within MMJJ_RS09015 at 1664.094 kb on + strand, within MMJJ_RS09015 at 1664.133 kb on - strand at 1664.134 kb on + strand at 1664.136 kb on - strand at 1664.136 kb on - strand at 1664.136 kb on - strand at 1664.137 kb on + strand at 1664.139 kb on - strand at 1664.142 kb on - strand at 1664.143 kb on + strand at 1664.155 kb on + strand at 1664.156 kb on - strand at 1664.157 kb on + strand at 1664.171 kb on - strand at 1664.186 kb on - strand at 1664.186 kb on - strand at 1664.187 kb on + strand at 1664.187 kb on + strand at 1664.198 kb on - strand at 1664.211 kb on - strand at 1664.218 kb on - strand at 1664.219 kb on + strand at 1664.219 kb on + strand at 1664.219 kb on + strand at 1664.220 kb on - strand at 1664.221 kb on + strand at 1664.232 kb on - strand at 1664.232 kb on - strand at 1664.248 kb on + strand at 1664.254 kb on + strand at 1664.254 kb on + strand at 1664.265 kb on + strand, within MMJJ_RS09020 at 1664.268 kb on - strand, within MMJJ_RS09020 at 1664.269 kb on + strand, within MMJJ_RS09020 at 1664.334 kb on - strand, within MMJJ_RS09020 at 1664.338 kb on + strand, within MMJJ_RS09020 at 1664.458 kb on - strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.470 kb on + strand, within MMJJ_RS09020 at 1664.482 kb on + strand, within MMJJ_RS09020 at 1664.530 kb on - strand, within MMJJ_RS09020 at 1664.564 kb on - strand, within MMJJ_RS09020 at 1664.610 kb on - strand at 1664.612 kb on - strand at 1664.613 kb on + strand at 1664.619 kb on - strand at 1664.620 kb on + strand at 1664.621 kb on - strand at 1664.622 kb on + strand at 1664.622 kb on + strand at 1664.623 kb on - strand at 1664.623 kb on - strand at 1664.628 kb on - strand at 1664.628 kb on - strand at 1664.628 kb on - strand at 1664.629 kb on + strand at 1664.629 kb on + strand at 1664.629 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.665 kb on - strand at 1664.665 kb on - strand at 1664.666 kb on + strand at 1664.667 kb on - strand at 1664.667 kb on - strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.675 kb on - strand at 1664.675 kb on - strand at 1664.675 kb on - strand at 1664.676 kb on + strand at 1664.679 kb on - strand at 1664.680 kb on + strand at 1664.691 kb on - strand at 1664.700 kb on - strand at 1664.701 kb on + strand at 1664.702 kb on - strand at 1664.703 kb on + strand at 1664.703 kb on + strand at 1664.704 kb on - strand at 1664.704 kb on - strand at 1664.705 kb on + strand at 1664.709 kb on - strand at 1664.714 kb on - strand at 1664.717 kb on + strand at 1664.719 kb on - strand at 1665.157 kb on - strand at 1665.327 kb on + strand at 1665.355 kb on + strand at 1665.389 kb on - strand at 1665.390 kb on + strand at 1665.433 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.482 kb on + strand at 1665.494 kb on - strand at 1665.497 kb on - strand at 1665.501 kb on + strand at 1665.521 kb on - strand at 1665.587 kb on - strand at 1665.623 kb on - strand, within MMJJ_RS09030
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,663,235 - MMJJ_RS09010 0.86 +0.2 1,663,242 - MMJJ_RS09010 0.87 -2.6 1,663,255 - -2.8 1,663,255 - +3.1 1,663,255 - -0.6 1,663,255 - -0.7 1,663,255 - +2.2 1,663,255 - -3.2 1,663,256 + -1.6 1,663,256 + +3.0 1,663,256 + +0.3 1,663,259 - +2.4 1,663,259 - -0.3 1,663,271 + -0.8 1,663,294 - -1.7 1,663,294 - +1.3 1,663,294 - +0.1 1,663,302 - -2.8 1,663,304 - -0.4 1,663,329 + -0.4 1,663,378 + +0.2 1,663,464 - MMJJ_RS09015 0.10 -1.9 1,663,683 + MMJJ_RS09015 0.38 -3.6 1,663,767 - MMJJ_RS09015 0.48 +3.5 1,663,819 + MMJJ_RS09015 0.54 -1.2 1,663,830 - MMJJ_RS09015 0.56 +0.2 1,663,860 - MMJJ_RS09015 0.59 -2.1 1,663,862 - MMJJ_RS09015 0.60 -0.1 1,663,863 + MMJJ_RS09015 0.60 -0.4 1,663,906 + MMJJ_RS09015 0.65 -3.1 1,663,925 + MMJJ_RS09015 0.68 -0.1 1,663,964 - MMJJ_RS09015 0.72 -1.8 1,663,993 + MMJJ_RS09015 0.76 +0.0 1,663,998 + MMJJ_RS09015 0.77 +3.6 1,664,004 - MMJJ_RS09015 0.77 -0.3 1,664,004 - MMJJ_RS09015 0.77 -2.4 1,664,004 - MMJJ_RS09015 0.77 -3.3 1,664,004 - MMJJ_RS09015 0.77 -1.8 1,664,004 - MMJJ_RS09015 0.77 -0.2 1,664,004 - MMJJ_RS09015 0.77 -1.8 1,664,005 + MMJJ_RS09015 0.77 -0.4 1,664,005 + MMJJ_RS09015 0.77 -1.8 1,664,005 + MMJJ_RS09015 0.77 -0.8 1,664,010 - MMJJ_RS09015 0.78 -1.8 1,664,010 - MMJJ_RS09015 0.78 -0.4 1,664,010 - MMJJ_RS09015 0.78 +1.1 1,664,016 - MMJJ_RS09015 0.79 +3.7 1,664,016 - MMJJ_RS09015 0.79 +0.9 1,664,016 - MMJJ_RS09015 0.79 -2.0 1,664,022 - MMJJ_RS09015 0.80 +0.2 1,664,023 + MMJJ_RS09015 0.80 -2.5 1,664,040 - MMJJ_RS09015 0.82 -1.9 1,664,040 - MMJJ_RS09015 0.82 +1.2 1,664,049 + MMJJ_RS09015 0.83 -1.1 1,664,091 - MMJJ_RS09015 0.88 +0.2 1,664,093 - MMJJ_RS09015 0.88 +1.7 1,664,093 - MMJJ_RS09015 0.88 +1.6 1,664,094 + MMJJ_RS09015 0.88 +1.7 1,664,133 - +2.3 1,664,134 + +3.3 1,664,136 - +2.2 1,664,136 - +0.5 1,664,136 - +3.7 1,664,137 + +1.1 1,664,139 - +0.2 1,664,142 - -1.4 1,664,143 + +2.6 1,664,155 + +0.2 1,664,156 - -0.4 1,664,157 + -0.8 1,664,171 - +3.3 1,664,186 - -2.2 1,664,186 - +1.7 1,664,187 + -0.4 1,664,187 + -0.5 1,664,198 - +2.0 1,664,211 - -2.5 1,664,218 - +1.2 1,664,219 + +2.2 1,664,219 + +2.3 1,664,219 + -0.6 1,664,220 - -1.4 1,664,221 + +1.9 1,664,232 - +1.0 1,664,232 - -2.3 1,664,248 + +3.7 1,664,254 + -1.7 1,664,254 + -2.6 1,664,265 + MMJJ_RS09020 0.10 -2.2 1,664,268 - MMJJ_RS09020 0.11 +1.1 1,664,269 + MMJJ_RS09020 0.11 +0.2 1,664,334 - MMJJ_RS09020 0.27 +0.3 1,664,338 + MMJJ_RS09020 0.28 +2.5 1,664,458 - MMJJ_RS09020 0.57 +3.7 1,664,459 + MMJJ_RS09020 0.57 +1.2 1,664,459 + MMJJ_RS09020 0.57 +2.9 1,664,459 + MMJJ_RS09020 0.57 -0.0 1,664,470 + MMJJ_RS09020 0.60 +2.1 1,664,482 + MMJJ_RS09020 0.63 -3.4 1,664,530 - MMJJ_RS09020 0.74 +1.4 1,664,564 - MMJJ_RS09020 0.82 +1.5 1,664,610 - -0.2 1,664,612 - +1.1 1,664,613 + -1.7 1,664,619 - -0.1 1,664,620 + -3.2 1,664,621 - +0.7 1,664,622 + -0.5 1,664,622 + -0.6 1,664,623 - +0.6 1,664,623 - -0.9 1,664,628 - -1.7 1,664,628 - -0.8 1,664,628 - -0.1 1,664,629 + -1.8 1,664,629 + +0.9 1,664,629 + -3.6 1,664,664 + +1.2 1,664,664 + -1.1 1,664,664 + -1.6 1,664,664 + -1.6 1,664,665 - -1.4 1,664,665 - +3.5 1,664,666 + -3.5 1,664,667 - +1.5 1,664,667 - -0.8 1,664,668 + -3.5 1,664,668 + -2.2 1,664,668 + -1.9 1,664,668 + +2.4 1,664,675 - +4.3 1,664,675 - -2.8 1,664,675 - -0.8 1,664,676 + -1.9 1,664,679 - -1.7 1,664,680 + +1.0 1,664,691 - -1.8 1,664,700 - -1.6 1,664,701 + -3.0 1,664,702 - +2.9 1,664,703 + +0.1 1,664,703 + -2.5 1,664,704 - +0.5 1,664,704 - -1.9 1,664,705 + -1.4 1,664,709 - -1.4 1,664,714 - -1.4 1,664,717 + -0.3 1,664,719 - -0.8 1,665,157 - +1.2 1,665,327 + +0.2 1,665,355 + -0.4 1,665,389 - -2.7 1,665,390 + -0.8 1,665,433 - +0.2 1,665,481 - -0.4 1,665,481 - +0.5 1,665,481 - -2.2 1,665,481 - -0.8 1,665,481 - -1.3 1,665,481 - +1.8 1,665,481 - -2.4 1,665,481 - -0.6 1,665,482 + +1.2 1,665,494 - -2.5 1,665,497 - +1.2 1,665,501 + +3.0 1,665,521 - +0.2 1,665,587 - +1.2 1,665,623 - MMJJ_RS09030 0.10 -0.4
Or see this region's nucleotide sequence