Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08930 and MMJJ_RS08935 are separated by 76 nucleotides MMJJ_RS08935 and MMJJ_RS08940 are separated by 35 nucleotides MMJJ_RS08940 and MMJJ_RS09375 are separated by 13 nucleotides MMJJ_RS09375 and MMJJ_RS08945 are separated by 7 nucleotides MMJJ_RS08945 and MMJJ_RS08950 are separated by 55 nucleotides
MMJJ_RS08930: MMJJ_RS08930 - 5'/3'-nucleotidase SurE, at 1,647,196 to 1,647,990
_RS08930
MMJJ_RS08935: MMJJ_RS08935 - TIGR00288 family NYN domain-containing protein, at 1,648,067 to 1,648,714
_RS08935
MMJJ_RS08940: MMJJ_RS08940 - monovalent cation/H+ antiporter complex subunit F, at 1,648,750 to 1,649,019
_RS08940
MMJJ_RS09375: MMJJ_RS09375 - hypothetical protein, at 1,649,033 to 1,649,209
_RS09375
MMJJ_RS08945: MMJJ_RS08945 - DUF61 family protein, at 1,649,217 to 1,649,606
_RS08945
MMJJ_RS08950: MMJJ_RS08950 - DUF22 domain-containing protein, at 1,649,662 to 1,650,027
_RS08950
Position (kb)
1648
1649 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1647.073 kb on + strand at 1647.094 kb on - strand at 1647.095 kb on + strand at 1647.099 kb on - strand at 1647.108 kb on - strand at 1647.121 kb on - strand at 1647.121 kb on - strand at 1647.121 kb on - strand at 1647.121 kb on - strand at 1647.122 kb on + strand at 1647.122 kb on + strand at 1647.126 kb on - strand at 1647.131 kb on + strand at 1647.137 kb on - strand at 1647.137 kb on - strand at 1647.137 kb on - strand at 1647.140 kb on - strand at 1647.140 kb on - strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.149 kb on - strand at 1647.150 kb on + strand at 1647.150 kb on + strand at 1647.155 kb on - strand at 1647.156 kb on + strand at 1647.156 kb on + strand at 1647.173 kb on - strand at 1647.202 kb on + strand at 1647.218 kb on - strand at 1647.251 kb on - strand at 1647.270 kb on + strand at 1647.270 kb on + strand at 1647.270 kb on + strand at 1647.270 kb on + strand at 1647.341 kb on - strand, within MMJJ_RS08930 at 1647.360 kb on - strand, within MMJJ_RS08930 at 1647.360 kb on - strand, within MMJJ_RS08930 at 1647.401 kb on + strand, within MMJJ_RS08930 at 1647.407 kb on - strand, within MMJJ_RS08930 at 1647.438 kb on + strand, within MMJJ_RS08930 at 1647.444 kb on + strand, within MMJJ_RS08930 at 1647.472 kb on - strand, within MMJJ_RS08930 at 1647.472 kb on - strand, within MMJJ_RS08930 at 1647.476 kb on - strand, within MMJJ_RS08930 at 1647.477 kb on + strand, within MMJJ_RS08930 at 1647.519 kb on - strand, within MMJJ_RS08930 at 1647.723 kb on + strand, within MMJJ_RS08930 at 1647.751 kb on + strand, within MMJJ_RS08930 at 1647.783 kb on - strand, within MMJJ_RS08930 at 1647.787 kb on - strand, within MMJJ_RS08930 at 1647.788 kb on + strand, within MMJJ_RS08930 at 1647.788 kb on + strand, within MMJJ_RS08930 at 1647.884 kb on - strand, within MMJJ_RS08930 at 1647.885 kb on + strand, within MMJJ_RS08930 at 1647.885 kb on + strand, within MMJJ_RS08930 at 1647.886 kb on - strand, within MMJJ_RS08930 at 1647.886 kb on - strand, within MMJJ_RS08930 at 1647.887 kb on + strand, within MMJJ_RS08930 at 1647.927 kb on - strand at 1647.928 kb on + strand at 1647.939 kb on - strand at 1648.014 kb on + strand at 1648.023 kb on + strand at 1648.064 kb on - strand at 1648.367 kb on + strand, within MMJJ_RS08935 at 1648.515 kb on - strand at 1648.589 kb on + strand, within MMJJ_RS08935 at 1648.672 kb on - strand at 1648.672 kb on - strand at 1648.696 kb on + strand at 1648.733 kb on - strand at 1648.734 kb on + strand at 1648.734 kb on + strand at 1648.738 kb on + strand at 1648.742 kb on - strand at 1648.751 kb on - strand at 1648.752 kb on + strand at 1648.754 kb on + strand at 1648.877 kb on + strand, within MMJJ_RS08940 at 1648.924 kb on - strand, within MMJJ_RS08940 at 1648.925 kb on + strand, within MMJJ_RS08940 at 1649.000 kb on - strand at 1649.006 kb on + strand at 1649.030 kb on + strand at 1649.071 kb on - strand, within MMJJ_RS09375 at 1649.105 kb on - strand, within MMJJ_RS09375 at 1649.107 kb on - strand at 1649.112 kb on + strand at 1649.123 kb on - strand, within MMJJ_RS09375 at 1649.218 kb on - strand at 1649.222 kb on - strand at 1649.229 kb on - strand at 1649.238 kb on - strand at 1649.252 kb on - strand at 1649.253 kb on + strand at 1649.304 kb on - strand, within MMJJ_RS08945 at 1649.306 kb on - strand, within MMJJ_RS08945 at 1649.307 kb on + strand, within MMJJ_RS08945 at 1649.380 kb on + strand at 1649.418 kb on - strand, within MMJJ_RS08945 at 1649.419 kb on + strand, within MMJJ_RS08945 at 1649.419 kb on + strand, within MMJJ_RS08945 at 1649.419 kb on + strand, within MMJJ_RS08945 at 1649.421 kb on - strand, within MMJJ_RS08945 at 1649.424 kb on - strand, within MMJJ_RS08945 at 1649.424 kb on - strand, within MMJJ_RS08945 at 1649.425 kb on + strand, within MMJJ_RS08945 at 1649.425 kb on + strand, within MMJJ_RS08945 at 1649.436 kb on - strand, within MMJJ_RS08945 at 1649.436 kb on - strand, within MMJJ_RS08945 at 1649.437 kb on + strand, within MMJJ_RS08945 at 1649.437 kb on + strand, within MMJJ_RS08945 at 1649.509 kb on + strand, within MMJJ_RS08945 at 1649.523 kb on + strand, within MMJJ_RS08945 at 1649.624 kb on + strand at 1649.634 kb on + strand at 1649.644 kb on + strand at 1649.655 kb on - strand at 1649.655 kb on - strand at 1649.659 kb on - strand at 1649.659 kb on - strand at 1649.660 kb on + strand at 1649.678 kb on + strand at 1649.680 kb on - strand at 1649.680 kb on - strand at 1649.680 kb on - strand at 1649.681 kb on + strand at 1649.683 kb on - strand at 1649.683 kb on - strand at 1649.684 kb on + strand at 1649.684 kb on + strand at 1649.710 kb on + strand, within MMJJ_RS08950 at 1649.712 kb on - strand, within MMJJ_RS08950 at 1649.713 kb on + strand, within MMJJ_RS08950
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,647,073 + -0.4 1,647,094 - +0.4 1,647,095 + -0.9 1,647,099 - +2.8 1,647,108 - -0.4 1,647,121 - +0.5 1,647,121 - +0.0 1,647,121 - +5.8 1,647,121 - +0.4 1,647,122 + +1.2 1,647,122 + -1.9 1,647,126 - +0.2 1,647,131 + -3.9 1,647,137 - +0.1 1,647,137 - +1.5 1,647,137 - -0.4 1,647,140 - -0.3 1,647,140 - +2.4 1,647,141 + +0.9 1,647,141 + -1.8 1,647,141 + -0.5 1,647,141 + -1.4 1,647,141 + -3.8 1,647,149 - +0.2 1,647,150 + -0.1 1,647,150 + +0.6 1,647,155 - -1.6 1,647,156 + +4.0 1,647,156 + -3.0 1,647,173 - -3.3 1,647,202 + +1.8 1,647,218 - -0.4 1,647,251 - +2.6 1,647,270 + -1.1 1,647,270 + -0.4 1,647,270 + -1.8 1,647,270 + -1.4 1,647,341 - MMJJ_RS08930 0.18 +0.1 1,647,360 - MMJJ_RS08930 0.21 -0.7 1,647,360 - MMJJ_RS08930 0.21 +2.3 1,647,401 + MMJJ_RS08930 0.26 +1.4 1,647,407 - MMJJ_RS08930 0.27 +0.2 1,647,438 + MMJJ_RS08930 0.30 -2.1 1,647,444 + MMJJ_RS08930 0.31 -2.5 1,647,472 - MMJJ_RS08930 0.35 -2.3 1,647,472 - MMJJ_RS08930 0.35 -1.0 1,647,476 - MMJJ_RS08930 0.35 -2.6 1,647,477 + MMJJ_RS08930 0.35 -0.3 1,647,519 - MMJJ_RS08930 0.41 +1.1 1,647,723 + MMJJ_RS08930 0.66 +0.9 1,647,751 + MMJJ_RS08930 0.70 -2.1 1,647,783 - MMJJ_RS08930 0.74 -1.2 1,647,787 - MMJJ_RS08930 0.74 -2.6 1,647,788 + MMJJ_RS08930 0.74 +0.2 1,647,788 + MMJJ_RS08930 0.74 -1.4 1,647,884 - MMJJ_RS08930 0.87 -0.5 1,647,885 + MMJJ_RS08930 0.87 +0.7 1,647,885 + MMJJ_RS08930 0.87 +0.4 1,647,886 - MMJJ_RS08930 0.87 -1.5 1,647,886 - MMJJ_RS08930 0.87 -1.8 1,647,887 + MMJJ_RS08930 0.87 -0.3 1,647,927 - -2.0 1,647,928 + -0.8 1,647,939 - -0.8 1,648,014 + +0.2 1,648,023 + +1.2 1,648,064 - -1.1 1,648,367 + MMJJ_RS08935 0.46 +1.2 1,648,515 - +1.2 1,648,589 + MMJJ_RS08935 0.81 -2.6 1,648,672 - -2.1 1,648,672 - -0.7 1,648,696 + -0.6 1,648,733 - +4.5 1,648,734 + -1.8 1,648,734 + -1.4 1,648,738 + +0.2 1,648,742 - -0.6 1,648,751 - +3.4 1,648,752 + -2.9 1,648,754 + +3.8 1,648,877 + MMJJ_RS08940 0.47 +0.6 1,648,924 - MMJJ_RS08940 0.64 +0.2 1,648,925 + MMJJ_RS08940 0.65 -0.8 1,649,000 - +0.2 1,649,006 + +0.7 1,649,030 + -0.4 1,649,071 - MMJJ_RS09375 0.21 +0.0 1,649,105 - MMJJ_RS09375 0.41 -1.4 1,649,107 - +0.2 1,649,112 + +0.2 1,649,123 - MMJJ_RS09375 0.51 -0.8 1,649,218 - -0.9 1,649,222 - +2.2 1,649,229 - -1.1 1,649,238 - -1.6 1,649,252 - -1.2 1,649,253 + -2.1 1,649,304 - MMJJ_RS08945 0.22 -2.5 1,649,306 - MMJJ_RS08945 0.23 +0.2 1,649,307 + MMJJ_RS08945 0.23 -0.6 1,649,380 + -0.4 1,649,418 - MMJJ_RS08945 0.52 +1.0 1,649,419 + MMJJ_RS08945 0.52 -2.7 1,649,419 + MMJJ_RS08945 0.52 +0.3 1,649,419 + MMJJ_RS08945 0.52 -0.0 1,649,421 - MMJJ_RS08945 0.52 +0.2 1,649,424 - MMJJ_RS08945 0.53 -0.9 1,649,424 - MMJJ_RS08945 0.53 -1.4 1,649,425 + MMJJ_RS08945 0.53 +1.7 1,649,425 + MMJJ_RS08945 0.53 -0.4 1,649,436 - MMJJ_RS08945 0.56 -3.1 1,649,436 - MMJJ_RS08945 0.56 +1.0 1,649,437 + MMJJ_RS08945 0.56 -0.8 1,649,437 + MMJJ_RS08945 0.56 -2.6 1,649,509 + MMJJ_RS08945 0.75 -0.8 1,649,523 + MMJJ_RS08945 0.78 -2.2 1,649,624 + -0.5 1,649,634 + -1.5 1,649,644 + +3.3 1,649,655 - -1.8 1,649,655 - -0.5 1,649,659 - +3.9 1,649,659 - -2.4 1,649,660 + -1.6 1,649,678 + -1.6 1,649,680 - -1.2 1,649,680 - +0.2 1,649,680 - -2.2 1,649,681 + +0.2 1,649,683 - -1.1 1,649,683 - -0.6 1,649,684 + +3.2 1,649,684 + +1.7 1,649,710 + MMJJ_RS08950 0.13 -1.9 1,649,712 - MMJJ_RS08950 0.14 +0.2 1,649,713 + MMJJ_RS08950 0.14 -1.6
Or see this region's nucleotide sequence