Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08905 and MMJJ_RS08910 are separated by 22 nucleotides MMJJ_RS08910 and MMJJ_RS08915 are separated by 131 nucleotides MMJJ_RS08915 and MMJJ_RS08920 are separated by 34 nucleotides
MMJJ_RS08905: MMJJ_RS08905 - YcaO-related McrA-glycine thioamidation protein, at 1,642,889 to 1,644,091
_RS08905
MMJJ_RS08910: MMJJ_RS08910 - DUF167 domain-containing protein, at 1,644,114 to 1,644,419
_RS08910
MMJJ_RS08915: MMJJ_RS08915 - CoB--CoM heterodisulfide reductase subunit C, at 1,644,551 to 1,645,105
_RS08915
MMJJ_RS08920: MMJJ_RS08920 - CoB--CoM heterodisulfide reductase subunit B, at 1,645,140 to 1,646,021
_RS08920
Position (kb)
1644
1645 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1643.122 kb on - strand, within MMJJ_RS08905 at 1643.131 kb on - strand, within MMJJ_RS08905 at 1643.132 kb on + strand, within MMJJ_RS08905 at 1643.152 kb on - strand, within MMJJ_RS08905 at 1643.164 kb on - strand, within MMJJ_RS08905 at 1643.164 kb on - strand, within MMJJ_RS08905 at 1643.324 kb on + strand, within MMJJ_RS08905 at 1643.408 kb on - strand, within MMJJ_RS08905 at 1643.409 kb on + strand, within MMJJ_RS08905 at 1643.414 kb on + strand, within MMJJ_RS08905 at 1643.416 kb on - strand, within MMJJ_RS08905 at 1643.486 kb on - strand, within MMJJ_RS08905 at 1643.496 kb on - strand, within MMJJ_RS08905 at 1643.496 kb on - strand, within MMJJ_RS08905 at 1643.687 kb on + strand, within MMJJ_RS08905 at 1643.687 kb on + strand, within MMJJ_RS08905 at 1643.687 kb on + strand, within MMJJ_RS08905 at 1643.719 kb on - strand, within MMJJ_RS08905 at 1643.750 kb on - strand, within MMJJ_RS08905 at 1643.750 kb on - strand, within MMJJ_RS08905 at 1643.751 kb on + strand, within MMJJ_RS08905 at 1643.792 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.899 kb on + strand, within MMJJ_RS08905 at 1643.899 kb on + strand, within MMJJ_RS08905 at 1643.966 kb on - strand, within MMJJ_RS08905 at 1643.966 kb on - strand, within MMJJ_RS08905 at 1643.966 kb on - strand, within MMJJ_RS08905 at 1643.967 kb on + strand, within MMJJ_RS08905 at 1644.008 kb on - strand at 1644.009 kb on + strand at 1644.011 kb on - strand at 1644.055 kb on - strand at 1644.055 kb on - strand at 1644.055 kb on - strand at 1644.055 kb on - strand at 1644.056 kb on + strand at 1644.056 kb on + strand at 1644.056 kb on + strand at 1644.058 kb on + strand at 1644.072 kb on - strand at 1644.072 kb on - strand at 1644.076 kb on - strand at 1644.076 kb on - strand at 1644.076 kb on - strand at 1644.077 kb on + strand at 1644.077 kb on + strand at 1644.102 kb on - strand at 1644.103 kb on + strand at 1644.125 kb on - strand at 1644.126 kb on + strand at 1644.126 kb on + strand at 1644.147 kb on - strand, within MMJJ_RS08910 at 1644.167 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.180 kb on - strand, within MMJJ_RS08910 at 1644.181 kb on + strand, within MMJJ_RS08910 at 1644.219 kb on - strand, within MMJJ_RS08910 at 1644.219 kb on - strand, within MMJJ_RS08910 at 1644.220 kb on + strand, within MMJJ_RS08910 at 1644.253 kb on + strand, within MMJJ_RS08910 at 1644.293 kb on - strand, within MMJJ_RS08910 at 1644.301 kb on + strand, within MMJJ_RS08910 at 1644.306 kb on - strand, within MMJJ_RS08910 at 1644.359 kb on - strand, within MMJJ_RS08910 at 1644.360 kb on + strand, within MMJJ_RS08910 at 1644.360 kb on + strand, within MMJJ_RS08910 at 1644.362 kb on - strand, within MMJJ_RS08910 at 1644.379 kb on + strand, within MMJJ_RS08910 at 1644.433 kb on - strand at 1644.434 kb on + strand at 1644.438 kb on + strand at 1644.440 kb on - strand at 1644.455 kb on - strand at 1644.456 kb on + strand at 1644.456 kb on + strand at 1644.495 kb on + strand at 1644.507 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.512 kb on + strand at 1644.512 kb on + strand at 1644.513 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.517 kb on + strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.519 kb on + strand at 1644.519 kb on + strand at 1644.519 kb on + strand at 1644.520 kb on - strand at 1644.520 kb on - strand at 1644.521 kb on + strand at 1644.521 kb on + strand at 1644.521 kb on + strand at 1644.670 kb on - strand, within MMJJ_RS08915 at 1644.723 kb on - strand, within MMJJ_RS08915 at 1644.723 kb on - strand, within MMJJ_RS08915 at 1644.724 kb on + strand, within MMJJ_RS08915 at 1644.781 kb on - strand, within MMJJ_RS08915 at 1644.906 kb on - strand, within MMJJ_RS08915 at 1644.906 kb on - strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.908 kb on - strand, within MMJJ_RS08915 at 1644.908 kb on - strand, within MMJJ_RS08915 at 1644.909 kb on + strand, within MMJJ_RS08915 at 1644.916 kb on + strand, within MMJJ_RS08915 at 1644.916 kb on + strand, within MMJJ_RS08915 at 1645.108 kb on - strand at 1645.109 kb on + strand at 1645.124 kb on - strand at 1645.178 kb on - strand at 1645.179 kb on + strand at 1645.179 kb on + strand at 1645.203 kb on + strand at 1645.241 kb on + strand, within MMJJ_RS08920 at 1645.359 kb on + strand, within MMJJ_RS08920 at 1645.359 kb on + strand, within MMJJ_RS08920 at 1645.370 kb on + strand, within MMJJ_RS08920 at 1645.370 kb on + strand, within MMJJ_RS08920 at 1645.370 kb on + strand, within MMJJ_RS08920 at 1645.414 kb on - strand, within MMJJ_RS08920
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,643,122 - MMJJ_RS08905 0.19 +1.2 1,643,131 - MMJJ_RS08905 0.20 +0.8 1,643,132 + MMJJ_RS08905 0.20 -2.4 1,643,152 - MMJJ_RS08905 0.22 +2.2 1,643,164 - MMJJ_RS08905 0.23 +1.7 1,643,164 - MMJJ_RS08905 0.23 +1.7 1,643,324 + MMJJ_RS08905 0.36 +1.9 1,643,408 - MMJJ_RS08905 0.43 -2.1 1,643,409 + MMJJ_RS08905 0.43 +2.2 1,643,414 + MMJJ_RS08905 0.44 -0.9 1,643,416 - MMJJ_RS08905 0.44 -1.4 1,643,486 - MMJJ_RS08905 0.50 -0.8 1,643,496 - MMJJ_RS08905 0.50 +0.5 1,643,496 - MMJJ_RS08905 0.50 -1.4 1,643,687 + MMJJ_RS08905 0.66 +0.5 1,643,687 + MMJJ_RS08905 0.66 +2.2 1,643,687 + MMJJ_RS08905 0.66 -1.6 1,643,719 - MMJJ_RS08905 0.69 +2.2 1,643,750 - MMJJ_RS08905 0.72 -0.4 1,643,750 - MMJJ_RS08905 0.72 +2.8 1,643,751 + MMJJ_RS08905 0.72 +2.8 1,643,792 - MMJJ_RS08905 0.75 +1.5 1,643,898 - MMJJ_RS08905 0.84 +0.1 1,643,898 - MMJJ_RS08905 0.84 -1.4 1,643,898 - MMJJ_RS08905 0.84 -1.1 1,643,898 - MMJJ_RS08905 0.84 -0.0 1,643,899 + MMJJ_RS08905 0.84 +0.8 1,643,899 + MMJJ_RS08905 0.84 +4.6 1,643,966 - MMJJ_RS08905 0.90 +2.8 1,643,966 - MMJJ_RS08905 0.90 +0.8 1,643,966 - MMJJ_RS08905 0.90 +1.4 1,643,967 + MMJJ_RS08905 0.90 -2.6 1,644,008 - -0.9 1,644,009 + -0.2 1,644,011 - -0.9 1,644,055 - -0.6 1,644,055 - +1.6 1,644,055 - +2.1 1,644,055 - -1.4 1,644,056 + -0.1 1,644,056 + +0.4 1,644,056 + -1.8 1,644,058 + -0.5 1,644,072 - +2.9 1,644,072 - -1.0 1,644,076 - -1.1 1,644,076 - +0.7 1,644,076 - +1.2 1,644,077 + -1.5 1,644,077 + -1.1 1,644,102 - +1.4 1,644,103 + -0.2 1,644,125 - +2.2 1,644,126 + -2.4 1,644,126 + -0.4 1,644,147 - MMJJ_RS08910 0.11 -3.2 1,644,167 - MMJJ_RS08910 0.17 +0.9 1,644,176 - MMJJ_RS08910 0.20 -1.7 1,644,176 - MMJJ_RS08910 0.20 -3.3 1,644,176 - MMJJ_RS08910 0.20 +0.0 1,644,176 - MMJJ_RS08910 0.20 -3.7 1,644,176 - MMJJ_RS08910 0.20 -0.7 1,644,176 - MMJJ_RS08910 0.20 -0.5 1,644,176 - MMJJ_RS08910 0.20 -2.2 1,644,177 + MMJJ_RS08910 0.21 -0.4 1,644,177 + MMJJ_RS08910 0.21 +1.2 1,644,177 + MMJJ_RS08910 0.21 +0.6 1,644,177 + MMJJ_RS08910 0.21 -1.0 1,644,180 - MMJJ_RS08910 0.22 +0.6 1,644,181 + MMJJ_RS08910 0.22 +1.2 1,644,219 - MMJJ_RS08910 0.34 +1.8 1,644,219 - MMJJ_RS08910 0.34 -0.3 1,644,220 + MMJJ_RS08910 0.35 -1.4 1,644,253 + MMJJ_RS08910 0.45 -0.8 1,644,293 - MMJJ_RS08910 0.58 -1.5 1,644,301 + MMJJ_RS08910 0.61 +1.4 1,644,306 - MMJJ_RS08910 0.63 -0.9 1,644,359 - MMJJ_RS08910 0.80 -3.0 1,644,360 + MMJJ_RS08910 0.80 -0.4 1,644,360 + MMJJ_RS08910 0.80 +0.3 1,644,362 - MMJJ_RS08910 0.81 -1.0 1,644,379 + MMJJ_RS08910 0.87 +0.9 1,644,433 - +0.1 1,644,434 + -2.2 1,644,438 + -0.2 1,644,440 - -0.9 1,644,455 - +1.2 1,644,456 + -2.7 1,644,456 + -0.5 1,644,495 + +2.0 1,644,507 - -1.6 1,644,511 - +1.7 1,644,511 - -0.0 1,644,511 - +2.6 1,644,511 - +1.5 1,644,511 - +2.2 1,644,511 - -1.1 1,644,512 + -0.8 1,644,512 + -0.3 1,644,513 - +1.2 1,644,516 - +0.3 1,644,516 - +1.3 1,644,516 - -1.4 1,644,516 - -0.2 1,644,516 - -0.4 1,644,517 + +4.1 1,644,518 - +1.0 1,644,518 - +0.1 1,644,518 - -2.7 1,644,518 - +4.1 1,644,518 - -1.8 1,644,519 + +1.6 1,644,519 + +1.2 1,644,519 + +0.9 1,644,520 - -0.8 1,644,520 - -2.5 1,644,521 + -0.4 1,644,521 + -0.8 1,644,521 + +2.0 1,644,670 - MMJJ_RS08915 0.21 +0.1 1,644,723 - MMJJ_RS08915 0.31 -1.9 1,644,723 - MMJJ_RS08915 0.31 +1.2 1,644,724 + MMJJ_RS08915 0.31 -1.0 1,644,781 - MMJJ_RS08915 0.41 -2.9 1,644,906 - MMJJ_RS08915 0.64 +0.3 1,644,906 - MMJJ_RS08915 0.64 +1.2 1,644,907 + MMJJ_RS08915 0.64 +1.3 1,644,907 + MMJJ_RS08915 0.64 -0.4 1,644,907 + MMJJ_RS08915 0.64 -0.6 1,644,907 + MMJJ_RS08915 0.64 -1.6 1,644,908 - MMJJ_RS08915 0.64 +1.2 1,644,908 - MMJJ_RS08915 0.64 +0.0 1,644,909 + MMJJ_RS08915 0.65 +1.6 1,644,916 + MMJJ_RS08915 0.66 +1.7 1,644,916 + MMJJ_RS08915 0.66 +0.4 1,645,108 - +5.2 1,645,109 + +0.6 1,645,124 - +0.8 1,645,178 - -3.9 1,645,179 + +2.0 1,645,179 + +0.6 1,645,203 + -1.9 1,645,241 + MMJJ_RS08920 0.11 +1.7 1,645,359 + MMJJ_RS08920 0.25 +0.0 1,645,359 + MMJJ_RS08920 0.25 -0.1 1,645,370 + MMJJ_RS08920 0.26 +0.4 1,645,370 + MMJJ_RS08920 0.26 +0.7 1,645,370 + MMJJ_RS08920 0.26 -0.0 1,645,414 - MMJJ_RS08920 0.31 -2.7
Or see this region's nucleotide sequence