Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08705 and MMJJ_RS08710 are separated by 73 nucleotides MMJJ_RS08710 and MMJJ_RS08715 are separated by 33 nucleotides MMJJ_RS08715 and MMJJ_RS08720 are separated by 15 nucleotides
MMJJ_RS08705: MMJJ_RS08705 - AEC family transporter, at 1,602,405 to 1,603,319
_RS08705
MMJJ_RS08710: MMJJ_RS08710 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,603,393 to 1,604,241
_RS08710
MMJJ_RS08715: MMJJ_RS08715 - NAD-dependent epimerase/dehydratase family protein, at 1,604,275 to 1,605,150
_RS08715
MMJJ_RS08720: MMJJ_RS08720 - UDP-glucose/GDP-mannose dehydrogenase family protein, at 1,605,166 to 1,606,488
_RS08720
Position (kb)
1604
1605
1606 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1603.279 kb on - strand at 1603.280 kb on + strand at 1603.280 kb on + strand at 1603.280 kb on + strand at 1603.301 kb on + strand at 1603.301 kb on + strand at 1603.305 kb on - strand at 1603.306 kb on + strand at 1603.312 kb on - strand at 1603.330 kb on - strand at 1603.345 kb on - strand at 1603.345 kb on - strand at 1603.363 kb on - strand at 1603.363 kb on - strand at 1603.364 kb on + strand at 1603.364 kb on + strand at 1603.366 kb on - strand at 1603.366 kb on - strand at 1603.367 kb on + strand at 1603.370 kb on + strand at 1603.370 kb on + strand at 1603.372 kb on + strand at 1603.392 kb on + strand at 1603.412 kb on - strand at 1603.499 kb on - strand, within MMJJ_RS08710 at 1603.500 kb on + strand, within MMJJ_RS08710 at 1603.500 kb on + strand, within MMJJ_RS08710 at 1603.500 kb on + strand, within MMJJ_RS08710 at 1603.504 kb on - strand, within MMJJ_RS08710 at 1603.504 kb on - strand, within MMJJ_RS08710 at 1603.504 kb on - strand, within MMJJ_RS08710 at 1603.505 kb on + strand, within MMJJ_RS08710 at 1603.509 kb on + strand, within MMJJ_RS08710 at 1603.548 kb on + strand, within MMJJ_RS08710 at 1603.551 kb on + strand, within MMJJ_RS08710 at 1603.555 kb on + strand, within MMJJ_RS08710 at 1603.662 kb on - strand, within MMJJ_RS08710 at 1603.663 kb on + strand, within MMJJ_RS08710 at 1603.665 kb on - strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.682 kb on - strand, within MMJJ_RS08710 at 1603.683 kb on + strand, within MMJJ_RS08710 at 1603.683 kb on + strand, within MMJJ_RS08710 at 1603.684 kb on - strand, within MMJJ_RS08710 at 1603.684 kb on - strand, within MMJJ_RS08710 at 1603.688 kb on + strand, within MMJJ_RS08710 at 1603.688 kb on + strand, within MMJJ_RS08710 at 1603.728 kb on + strand, within MMJJ_RS08710 at 1603.766 kb on - strand, within MMJJ_RS08710 at 1603.766 kb on - strand, within MMJJ_RS08710 at 1603.770 kb on + strand, within MMJJ_RS08710 at 1603.884 kb on - strand, within MMJJ_RS08710 at 1603.899 kb on + strand, within MMJJ_RS08710 at 1603.961 kb on - strand, within MMJJ_RS08710 at 1603.985 kb on - strand, within MMJJ_RS08710 at 1604.000 kb on + strand, within MMJJ_RS08710 at 1604.004 kb on + strand, within MMJJ_RS08710 at 1604.070 kb on - strand, within MMJJ_RS08710 at 1604.142 kb on - strand, within MMJJ_RS08710 at 1604.196 kb on - strand at 1604.196 kb on - strand at 1604.197 kb on + strand at 1604.197 kb on + strand at 1604.198 kb on - strand at 1604.199 kb on + strand at 1604.199 kb on + strand at 1604.199 kb on + strand at 1604.216 kb on + strand at 1604.246 kb on + strand at 1604.247 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.252 kb on + strand at 1604.252 kb on + strand at 1604.254 kb on - strand at 1604.254 kb on - strand at 1604.255 kb on + strand at 1604.259 kb on - strand at 1604.363 kb on - strand, within MMJJ_RS08715 at 1604.372 kb on - strand, within MMJJ_RS08715 at 1604.373 kb on + strand, within MMJJ_RS08715 at 1604.433 kb on + strand, within MMJJ_RS08715 at 1604.532 kb on - strand, within MMJJ_RS08715 at 1604.595 kb on - strand, within MMJJ_RS08715 at 1604.597 kb on - strand, within MMJJ_RS08715 at 1604.598 kb on + strand, within MMJJ_RS08715 at 1604.598 kb on + strand, within MMJJ_RS08715 at 1604.636 kb on + strand, within MMJJ_RS08715 at 1604.710 kb on - strand, within MMJJ_RS08715 at 1604.710 kb on - strand, within MMJJ_RS08715 at 1604.712 kb on - strand, within MMJJ_RS08715 at 1604.722 kb on - strand, within MMJJ_RS08715 at 1604.725 kb on + strand, within MMJJ_RS08715 at 1604.752 kb on + strand, within MMJJ_RS08715 at 1604.757 kb on + strand, within MMJJ_RS08715 at 1604.951 kb on - strand, within MMJJ_RS08715 at 1604.952 kb on + strand, within MMJJ_RS08715 at 1604.952 kb on + strand, within MMJJ_RS08715 at 1604.955 kb on - strand, within MMJJ_RS08715 at 1604.955 kb on - strand, within MMJJ_RS08715 at 1604.957 kb on - strand, within MMJJ_RS08715 at 1604.957 kb on - strand, within MMJJ_RS08715 at 1604.957 kb on - strand, within MMJJ_RS08715 at 1604.958 kb on + strand, within MMJJ_RS08715 at 1604.958 kb on + strand, within MMJJ_RS08715 at 1605.034 kb on + strand, within MMJJ_RS08715 at 1605.038 kb on + strand, within MMJJ_RS08715 at 1605.064 kb on - strand at 1605.071 kb on - strand at 1605.071 kb on - strand at 1605.072 kb on + strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.073 kb on - strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.074 kb on + strand at 1605.075 kb on - strand at 1605.075 kb on - strand at 1605.075 kb on - strand at 1605.076 kb on + strand at 1605.076 kb on + strand at 1605.101 kb on - strand at 1605.148 kb on - strand at 1605.153 kb on - strand at 1605.153 kb on - strand at 1605.154 kb on + strand at 1605.154 kb on + strand at 1605.193 kb on - strand at 1605.193 kb on - strand at 1605.203 kb on - strand at 1605.250 kb on + strand at 1605.282 kb on + strand at 1605.285 kb on - strand at 1605.304 kb on - strand, within MMJJ_RS08720 at 1605.304 kb on - strand, within MMJJ_RS08720 at 1605.304 kb on - strand, within MMJJ_RS08720 at 1605.304 kb on - strand, within MMJJ_RS08720 at 1605.305 kb on + strand, within MMJJ_RS08720 at 1605.305 kb on + strand, within MMJJ_RS08720 at 1605.305 kb on + strand, within MMJJ_RS08720 at 1605.381 kb on - strand, within MMJJ_RS08720 at 1605.382 kb on + strand, within MMJJ_RS08720 at 1605.512 kb on - strand, within MMJJ_RS08720 at 1605.512 kb on - strand, within MMJJ_RS08720 at 1605.513 kb on + strand, within MMJJ_RS08720 at 1605.582 kb on - strand, within MMJJ_RS08720 at 1605.582 kb on - strand, within MMJJ_RS08720 at 1605.647 kb on - strand, within MMJJ_RS08720 at 1605.647 kb on - strand, within MMJJ_RS08720 at 1605.701 kb on - strand, within MMJJ_RS08720 at 1605.702 kb on + strand, within MMJJ_RS08720 at 1605.702 kb on + strand, within MMJJ_RS08720 at 1605.702 kb on + strand, within MMJJ_RS08720 at 1605.748 kb on - strand, within MMJJ_RS08720 at 1605.748 kb on - strand, within MMJJ_RS08720 at 1605.749 kb on + strand, within MMJJ_RS08720 at 1605.768 kb on + strand, within MMJJ_RS08720 at 1605.772 kb on - strand, within MMJJ_RS08720 at 1605.773 kb on + strand, within MMJJ_RS08720 at 1605.813 kb on + strand, within MMJJ_RS08720 at 1605.854 kb on - strand, within MMJJ_RS08720 at 1605.855 kb on + strand, within MMJJ_RS08720 at 1605.863 kb on - strand, within MMJJ_RS08720 at 1605.863 kb on - strand, within MMJJ_RS08720 at 1605.868 kb on - strand, within MMJJ_RS08720 at 1605.869 kb on + strand, within MMJJ_RS08720 at 1605.953 kb on - strand, within MMJJ_RS08720 at 1605.979 kb on - strand, within MMJJ_RS08720 at 1605.981 kb on - strand, within MMJJ_RS08720 at 1605.982 kb on + strand, within MMJJ_RS08720 at 1605.982 kb on + strand, within MMJJ_RS08720 at 1605.982 kb on + strand, within MMJJ_RS08720 at 1605.982 kb on + strand, within MMJJ_RS08720 at 1606.002 kb on - strand, within MMJJ_RS08720 at 1606.017 kb on - strand, within MMJJ_RS08720 at 1606.018 kb on + strand, within MMJJ_RS08720 at 1606.023 kb on + strand, within MMJJ_RS08720 at 1606.037 kb on - strand, within MMJJ_RS08720 at 1606.038 kb on + strand, within MMJJ_RS08720 at 1606.066 kb on - strand, within MMJJ_RS08720 at 1606.067 kb on + strand, within MMJJ_RS08720 at 1606.070 kb on + strand, within MMJJ_RS08720 at 1606.079 kb on - strand, within MMJJ_RS08720 at 1606.079 kb on - strand, within MMJJ_RS08720 at 1606.080 kb on + strand, within MMJJ_RS08720 at 1606.086 kb on - strand, within MMJJ_RS08720
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,603,279 - +1.5 1,603,280 + +1.9 1,603,280 + +1.7 1,603,280 + +1.6 1,603,301 + +0.5 1,603,301 + -0.6 1,603,305 - -1.8 1,603,306 + +4.0 1,603,312 - +3.8 1,603,330 - +2.7 1,603,345 - -1.6 1,603,345 - +4.9 1,603,363 - +4.3 1,603,363 - -1.1 1,603,364 + +2.3 1,603,364 + -1.8 1,603,366 - +5.3 1,603,366 - +2.2 1,603,367 + -0.8 1,603,370 + -0.8 1,603,370 + -0.8 1,603,372 + +6.6 1,603,392 + +3.4 1,603,412 - -0.8 1,603,499 - MMJJ_RS08710 0.12 +1.2 1,603,500 + MMJJ_RS08710 0.13 +5.3 1,603,500 + MMJJ_RS08710 0.13 +4.8 1,603,500 + MMJJ_RS08710 0.13 -1.6 1,603,504 - MMJJ_RS08710 0.13 -2.2 1,603,504 - MMJJ_RS08710 0.13 -0.0 1,603,504 - MMJJ_RS08710 0.13 -1.1 1,603,505 + MMJJ_RS08710 0.13 -2.4 1,603,509 + MMJJ_RS08710 0.14 +5.5 1,603,548 + MMJJ_RS08710 0.18 +2.1 1,603,551 + MMJJ_RS08710 0.19 +5.4 1,603,555 + MMJJ_RS08710 0.19 -4.2 1,603,662 - MMJJ_RS08710 0.32 -2.6 1,603,663 + MMJJ_RS08710 0.32 +1.6 1,603,665 - MMJJ_RS08710 0.32 +2.1 1,603,681 + MMJJ_RS08710 0.34 -1.2 1,603,681 + MMJJ_RS08710 0.34 +1.0 1,603,681 + MMJJ_RS08710 0.34 +1.2 1,603,681 + MMJJ_RS08710 0.34 +5.0 1,603,682 - MMJJ_RS08710 0.34 -0.8 1,603,683 + MMJJ_RS08710 0.34 +2.7 1,603,683 + MMJJ_RS08710 0.34 -2.4 1,603,684 - MMJJ_RS08710 0.34 -1.4 1,603,684 - MMJJ_RS08710 0.34 -1.9 1,603,688 + MMJJ_RS08710 0.35 +1.5 1,603,688 + MMJJ_RS08710 0.35 -1.1 1,603,728 + MMJJ_RS08710 0.39 -2.1 1,603,766 - MMJJ_RS08710 0.44 +0.2 1,603,766 - MMJJ_RS08710 0.44 +4.4 1,603,770 + MMJJ_RS08710 0.44 -0.8 1,603,884 - MMJJ_RS08710 0.58 -1.9 1,603,899 + MMJJ_RS08710 0.60 +2.3 1,603,961 - MMJJ_RS08710 0.67 -0.2 1,603,985 - MMJJ_RS08710 0.70 +3.9 1,604,000 + MMJJ_RS08710 0.71 +6.1 1,604,004 + MMJJ_RS08710 0.72 +1.9 1,604,070 - MMJJ_RS08710 0.80 +3.0 1,604,142 - MMJJ_RS08710 0.88 -2.8 1,604,196 - -1.6 1,604,196 - +0.4 1,604,197 + -2.9 1,604,197 + -1.1 1,604,198 - -0.8 1,604,199 + -0.8 1,604,199 + -0.4 1,604,199 + +3.2 1,604,216 + -2.2 1,604,246 + +2.1 1,604,247 - -0.5 1,604,251 - -2.4 1,604,251 - +1.5 1,604,251 - +1.0 1,604,251 - +1.5 1,604,251 - -1.9 1,604,252 + +0.4 1,604,252 + +4.1 1,604,254 - +2.1 1,604,254 - +0.2 1,604,255 + -2.9 1,604,259 - +3.3 1,604,363 - MMJJ_RS08715 0.10 +1.9 1,604,372 - MMJJ_RS08715 0.11 -1.9 1,604,373 + MMJJ_RS08715 0.11 -1.4 1,604,433 + MMJJ_RS08715 0.18 +3.8 1,604,532 - MMJJ_RS08715 0.29 -2.5 1,604,595 - MMJJ_RS08715 0.37 -0.8 1,604,597 - MMJJ_RS08715 0.37 +0.4 1,604,598 + MMJJ_RS08715 0.37 +0.5 1,604,598 + MMJJ_RS08715 0.37 -0.6 1,604,636 + MMJJ_RS08715 0.41 -2.6 1,604,710 - MMJJ_RS08715 0.50 -1.6 1,604,710 - MMJJ_RS08715 0.50 -1.6 1,604,712 - MMJJ_RS08715 0.50 +1.7 1,604,722 - MMJJ_RS08715 0.51 +0.6 1,604,725 + MMJJ_RS08715 0.51 -1.4 1,604,752 + MMJJ_RS08715 0.54 -2.2 1,604,757 + MMJJ_RS08715 0.55 -1.1 1,604,951 - MMJJ_RS08715 0.77 +3.1 1,604,952 + MMJJ_RS08715 0.77 +1.5 1,604,952 + MMJJ_RS08715 0.77 +1.2 1,604,955 - MMJJ_RS08715 0.78 -2.8 1,604,955 - MMJJ_RS08715 0.78 +1.6 1,604,957 - MMJJ_RS08715 0.78 +4.4 1,604,957 - MMJJ_RS08715 0.78 -0.4 1,604,957 - MMJJ_RS08715 0.78 +2.8 1,604,958 + MMJJ_RS08715 0.78 -2.4 1,604,958 + MMJJ_RS08715 0.78 -1.0 1,605,034 + MMJJ_RS08715 0.87 +0.1 1,605,038 + MMJJ_RS08715 0.87 -3.2 1,605,064 - -2.4 1,605,071 - -3.8 1,605,071 - -0.8 1,605,072 + -2.5 1,605,073 - -1.5 1,605,073 - +0.1 1,605,073 - -2.9 1,605,073 - +0.6 1,605,073 - +0.1 1,605,073 - -1.4 1,605,073 - -0.0 1,605,073 - +2.2 1,605,074 + +0.1 1,605,074 + +3.5 1,605,074 + +1.1 1,605,074 + -0.4 1,605,074 + -2.8 1,605,074 + +3.4 1,605,074 + -0.2 1,605,074 + -1.1 1,605,074 + +2.7 1,605,074 + +1.8 1,605,074 + +4.3 1,605,074 + +0.8 1,605,074 + +3.2 1,605,074 + -1.9 1,605,075 - -1.9 1,605,075 - -2.1 1,605,075 - +3.9 1,605,076 + +0.9 1,605,076 + -2.4 1,605,101 - -0.6 1,605,148 - +4.7 1,605,153 - -2.8 1,605,153 - -2.8 1,605,154 + -1.1 1,605,154 + +3.7 1,605,193 - +3.1 1,605,193 - -1.4 1,605,203 - -0.4 1,605,250 + +3.9 1,605,282 + +1.6 1,605,285 - -1.1 1,605,304 - MMJJ_RS08720 0.10 -2.8 1,605,304 - MMJJ_RS08720 0.10 +1.0 1,605,304 - MMJJ_RS08720 0.10 -1.1 1,605,304 - MMJJ_RS08720 0.10 +2.2 1,605,305 + MMJJ_RS08720 0.11 -1.0 1,605,305 + MMJJ_RS08720 0.11 -0.1 1,605,305 + MMJJ_RS08720 0.11 +3.6 1,605,381 - MMJJ_RS08720 0.16 +1.7 1,605,382 + MMJJ_RS08720 0.16 +5.8 1,605,512 - MMJJ_RS08720 0.26 +6.0 1,605,512 - MMJJ_RS08720 0.26 +0.2 1,605,513 + MMJJ_RS08720 0.26 -1.4 1,605,582 - MMJJ_RS08720 0.31 -1.1 1,605,582 - MMJJ_RS08720 0.31 -1.7 1,605,647 - MMJJ_RS08720 0.36 -1.4 1,605,647 - MMJJ_RS08720 0.36 -0.6 1,605,701 - MMJJ_RS08720 0.40 -1.6 1,605,702 + MMJJ_RS08720 0.41 +3.8 1,605,702 + MMJJ_RS08720 0.41 +0.2 1,605,702 + MMJJ_RS08720 0.41 -1.9 1,605,748 - MMJJ_RS08720 0.44 -1.8 1,605,748 - MMJJ_RS08720 0.44 -3.4 1,605,749 + MMJJ_RS08720 0.44 -1.8 1,605,768 + MMJJ_RS08720 0.46 -1.8 1,605,772 - MMJJ_RS08720 0.46 -0.4 1,605,773 + MMJJ_RS08720 0.46 -0.8 1,605,813 + MMJJ_RS08720 0.49 -2.0 1,605,854 - MMJJ_RS08720 0.52 -0.4 1,605,855 + MMJJ_RS08720 0.52 -1.8 1,605,863 - MMJJ_RS08720 0.53 -1.4 1,605,863 - MMJJ_RS08720 0.53 +2.3 1,605,868 - MMJJ_RS08720 0.53 -2.5 1,605,869 + MMJJ_RS08720 0.53 -0.9 1,605,953 - MMJJ_RS08720 0.59 +4.8 1,605,979 - MMJJ_RS08720 0.61 -0.8 1,605,981 - MMJJ_RS08720 0.62 -2.6 1,605,982 + MMJJ_RS08720 0.62 -1.6 1,605,982 + MMJJ_RS08720 0.62 -0.9 1,605,982 + MMJJ_RS08720 0.62 -0.2 1,605,982 + MMJJ_RS08720 0.62 -1.6 1,606,002 - MMJJ_RS08720 0.63 +3.0 1,606,017 - MMJJ_RS08720 0.64 +3.6 1,606,018 + MMJJ_RS08720 0.64 -1.4 1,606,023 + MMJJ_RS08720 0.65 +3.4 1,606,037 - MMJJ_RS08720 0.66 -2.6 1,606,038 + MMJJ_RS08720 0.66 -2.2 1,606,066 - MMJJ_RS08720 0.68 -2.2 1,606,067 + MMJJ_RS08720 0.68 -2.8 1,606,070 + MMJJ_RS08720 0.68 +1.9 1,606,079 - MMJJ_RS08720 0.69 -2.8 1,606,079 - MMJJ_RS08720 0.69 -2.6 1,606,080 + MMJJ_RS08720 0.69 -2.7 1,606,086 - MMJJ_RS08720 0.70 +5.6
Or see this region's nucleotide sequence