Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08610 and MMJJ_RS08615 overlap by 14 nucleotides MMJJ_RS08615 and MMJJ_RS08620 are separated by 28 nucleotides
MMJJ_RS08610: MMJJ_RS08610 - oligosaccharide repeat unit polymerase family protein, at 1,585,396 to 1,586,469
_RS08610
MMJJ_RS08615: MMJJ_RS08615 - oligosaccharide repeat unit polymerase family protein, at 1,586,456 to 1,587,631
_RS08615
MMJJ_RS08620: MMJJ_RS08620 - DUF373 family protein, at 1,587,660 to 1,588,769
_RS08620
Position (kb)
1586
1587
1588 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1585.726 kb on + strand, within MMJJ_RS08610 at 1586.417 kb on - strand at 1586.417 kb on - strand at 1586.533 kb on + strand at 1586.628 kb on - strand, within MMJJ_RS08615 at 1586.629 kb on + strand, within MMJJ_RS08615 at 1586.691 kb on - strand, within MMJJ_RS08615 at 1586.775 kb on - strand, within MMJJ_RS08615 at 1586.805 kb on - strand, within MMJJ_RS08615 at 1586.806 kb on + strand, within MMJJ_RS08615 at 1586.838 kb on + strand, within MMJJ_RS08615 at 1586.871 kb on - strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.967 kb on - strand, within MMJJ_RS08615 at 1587.020 kb on - strand, within MMJJ_RS08615 at 1587.031 kb on + strand, within MMJJ_RS08615 at 1587.081 kb on - strand, within MMJJ_RS08615 at 1587.082 kb on + strand, within MMJJ_RS08615 at 1587.103 kb on - strand, within MMJJ_RS08615 at 1587.153 kb on - strand, within MMJJ_RS08615 at 1587.154 kb on + strand, within MMJJ_RS08615 at 1587.157 kb on - strand at 1587.273 kb on - strand, within MMJJ_RS08615 at 1587.274 kb on + strand, within MMJJ_RS08615 at 1587.283 kb on - strand, within MMJJ_RS08615 at 1587.286 kb on + strand, within MMJJ_RS08615 at 1587.323 kb on + strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.373 kb on - strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.376 kb on - strand, within MMJJ_RS08615 at 1587.377 kb on + strand, within MMJJ_RS08615 at 1587.380 kb on + strand, within MMJJ_RS08615 at 1587.405 kb on + strand, within MMJJ_RS08615 at 1587.429 kb on - strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.460 kb on - strand, within MMJJ_RS08615 at 1587.465 kb on - strand, within MMJJ_RS08615 at 1587.466 kb on + strand, within MMJJ_RS08615 at 1587.471 kb on + strand, within MMJJ_RS08615 at 1587.514 kb on + strand at 1587.659 kb on + strand at 1587.671 kb on + strand at 1587.886 kb on - strand, within MMJJ_RS08620 at 1587.952 kb on - strand, within MMJJ_RS08620 at 1588.008 kb on + strand, within MMJJ_RS08620 at 1588.326 kb on + strand, within MMJJ_RS08620 at 1588.402 kb on - strand at 1588.408 kb on - strand, within MMJJ_RS08620 at 1588.445 kb on + strand, within MMJJ_RS08620 at 1588.475 kb on - strand, within MMJJ_RS08620 at 1588.476 kb on + strand, within MMJJ_RS08620 at 1588.528 kb on - strand, within MMJJ_RS08620 at 1588.540 kb on - strand, within MMJJ_RS08620
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,585,726 + MMJJ_RS08610 0.31 -0.1 1,586,417 - -0.4 1,586,417 - -0.5 1,586,533 + +1.8 1,586,628 - MMJJ_RS08615 0.15 +0.2 1,586,629 + MMJJ_RS08615 0.15 +1.5 1,586,691 - MMJJ_RS08615 0.20 -1.1 1,586,775 - MMJJ_RS08615 0.27 +0.2 1,586,805 - MMJJ_RS08615 0.30 -3.3 1,586,806 + MMJJ_RS08615 0.30 +1.7 1,586,838 + MMJJ_RS08615 0.32 +3.0 1,586,871 - MMJJ_RS08615 0.35 +4.2 1,586,881 + MMJJ_RS08615 0.36 +2.5 1,586,881 + MMJJ_RS08615 0.36 -1.1 1,586,905 + MMJJ_RS08615 0.38 -1.8 1,586,905 + MMJJ_RS08615 0.38 +2.7 1,586,967 - MMJJ_RS08615 0.43 -0.7 1,587,020 - MMJJ_RS08615 0.48 +1.2 1,587,031 + MMJJ_RS08615 0.49 -2.2 1,587,081 - MMJJ_RS08615 0.53 -2.5 1,587,082 + MMJJ_RS08615 0.53 +0.5 1,587,103 - MMJJ_RS08615 0.55 +0.2 1,587,153 - MMJJ_RS08615 0.59 -2.4 1,587,154 + MMJJ_RS08615 0.59 -2.7 1,587,157 - +0.2 1,587,273 - MMJJ_RS08615 0.69 +3.0 1,587,274 + MMJJ_RS08615 0.70 -0.8 1,587,283 - MMJJ_RS08615 0.70 +0.9 1,587,286 + MMJJ_RS08615 0.71 +3.0 1,587,323 + MMJJ_RS08615 0.74 -2.4 1,587,354 - MMJJ_RS08615 0.76 -1.8 1,587,354 - MMJJ_RS08615 0.76 +2.2 1,587,373 - MMJJ_RS08615 0.78 -1.6 1,587,374 + MMJJ_RS08615 0.78 -1.1 1,587,374 + MMJJ_RS08615 0.78 +1.7 1,587,374 + MMJJ_RS08615 0.78 -1.4 1,587,376 - MMJJ_RS08615 0.78 -2.1 1,587,377 + MMJJ_RS08615 0.78 -2.2 1,587,380 + MMJJ_RS08615 0.79 +3.4 1,587,405 + MMJJ_RS08615 0.81 +4.0 1,587,429 - MMJJ_RS08615 0.83 +3.9 1,587,445 + MMJJ_RS08615 0.84 +1.6 1,587,445 + MMJJ_RS08615 0.84 +0.4 1,587,460 - MMJJ_RS08615 0.85 +1.7 1,587,465 - MMJJ_RS08615 0.86 -1.0 1,587,466 + MMJJ_RS08615 0.86 +0.9 1,587,471 + MMJJ_RS08615 0.86 -1.4 1,587,514 + -2.1 1,587,659 + -2.2 1,587,671 + +0.2 1,587,886 - MMJJ_RS08620 0.20 +1.2 1,587,952 - MMJJ_RS08620 0.26 -0.8 1,588,008 + MMJJ_RS08620 0.31 -0.4 1,588,326 + MMJJ_RS08620 0.60 +1.3 1,588,402 - +0.2 1,588,408 - MMJJ_RS08620 0.67 -3.0 1,588,445 + MMJJ_RS08620 0.71 +1.2 1,588,475 - MMJJ_RS08620 0.73 -0.8 1,588,476 + MMJJ_RS08620 0.74 -1.8 1,588,528 - MMJJ_RS08620 0.78 -3.0 1,588,540 - MMJJ_RS08620 0.79 +0.6
Or see this region's nucleotide sequence