Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08320 and MMJJ_RS08325 are separated by 11 nucleotides MMJJ_RS08325 and MMJJ_RS08330 are separated by 149 nucleotides MMJJ_RS08330 and MMJJ_RS08335 overlap by 31 nucleotides MMJJ_RS08335 and MMJJ_RS08340 are separated by 0 nucleotides
MMJJ_RS08320: MMJJ_RS08320 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,533,989 to 1,534,603
_RS08320
MMJJ_RS08325: MMJJ_RS08325 - glycosyltransferase family 2 protein, at 1,534,615 to 1,535,307
_RS08325
MMJJ_RS08330: MMJJ_RS08330 - SufD family Fe-S cluster assembly protein, at 1,535,457 to 1,536,683
_RS08330
MMJJ_RS08335: MMJJ_RS08335 - ABC transporter ATP-binding protein, at 1,536,653 to 1,537,399
_RS08335
MMJJ_RS08340: MMJJ_RS08340 - archaeoflavoprotein AfpA, at 1,537,400 to 1,537,954
_RS08340
Position (kb)
1535
1536
1537 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1534.553 kb on - strand at 1534.756 kb on - strand, within MMJJ_RS08325 at 1534.782 kb on - strand, within MMJJ_RS08325 at 1534.993 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.244 kb on - strand at 1535.268 kb on - strand at 1535.344 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.370 kb on - strand at 1535.370 kb on - strand at 1535.372 kb on - strand at 1535.383 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.389 kb on - strand at 1535.406 kb on - strand at 1535.426 kb on - strand at 1535.427 kb on + strand at 1535.458 kb on - strand at 1535.599 kb on + strand, within MMJJ_RS08330 at 1535.764 kb on + strand, within MMJJ_RS08330 at 1535.783 kb on - strand, within MMJJ_RS08330 at 1535.872 kb on + strand, within MMJJ_RS08330 at 1535.899 kb on + strand, within MMJJ_RS08330 at 1536.153 kb on + strand, within MMJJ_RS08330 at 1536.156 kb on + strand, within MMJJ_RS08330 at 1536.411 kb on + strand, within MMJJ_RS08330 at 1536.805 kb on - strand, within MMJJ_RS08335 at 1536.806 kb on + strand, within MMJJ_RS08335 at 1536.826 kb on - strand, within MMJJ_RS08335 at 1536.977 kb on - strand, within MMJJ_RS08335 at 1537.427 kb on - strand at 1537.454 kb on - strand at 1537.499 kb on - strand, within MMJJ_RS08340 at 1537.517 kb on + strand, within MMJJ_RS08340 at 1537.518 kb on - strand, within MMJJ_RS08340 at 1537.518 kb on - strand, within MMJJ_RS08340 at 1537.520 kb on - strand, within MMJJ_RS08340 at 1537.520 kb on - strand, within MMJJ_RS08340 at 1537.520 kb on - strand, within MMJJ_RS08340 at 1537.521 kb on + strand, within MMJJ_RS08340 at 1537.556 kb on - strand, within MMJJ_RS08340 at 1537.556 kb on - strand, within MMJJ_RS08340 at 1537.591 kb on + strand, within MMJJ_RS08340
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,534,553 - +0.2 1,534,756 - MMJJ_RS08325 0.20 -1.4 1,534,782 - MMJJ_RS08325 0.24 -0.8 1,534,993 - MMJJ_RS08325 0.55 +0.6 1,535,200 - MMJJ_RS08325 0.84 -4.1 1,535,200 - MMJJ_RS08325 0.84 +1.2 1,535,244 - +2.0 1,535,268 - -0.8 1,535,344 - +2.6 1,535,357 - -1.4 1,535,357 - +1.2 1,535,357 - -0.4 1,535,370 - -1.5 1,535,370 - +2.6 1,535,372 - +1.9 1,535,383 + -0.2 1,535,386 + -0.6 1,535,386 + -1.6 1,535,386 + +2.9 1,535,386 + -0.0 1,535,389 - +0.9 1,535,406 - +3.0 1,535,426 - +0.6 1,535,427 + -1.8 1,535,458 - +1.5 1,535,599 + MMJJ_RS08330 0.12 +0.2 1,535,764 + MMJJ_RS08330 0.25 +0.2 1,535,783 - MMJJ_RS08330 0.27 -1.1 1,535,872 + MMJJ_RS08330 0.34 -2.1 1,535,899 + MMJJ_RS08330 0.36 -1.8 1,536,153 + MMJJ_RS08330 0.57 +1.2 1,536,156 + MMJJ_RS08330 0.57 -0.8 1,536,411 + MMJJ_RS08330 0.78 -0.4 1,536,805 - MMJJ_RS08335 0.20 +0.2 1,536,806 + MMJJ_RS08335 0.20 -2.6 1,536,826 - MMJJ_RS08335 0.23 +0.2 1,536,977 - MMJJ_RS08335 0.43 -1.6 1,537,427 - +0.0 1,537,454 - -1.4 1,537,499 - MMJJ_RS08340 0.18 -0.8 1,537,517 + MMJJ_RS08340 0.21 -1.6 1,537,518 - MMJJ_RS08340 0.21 -0.8 1,537,518 - MMJJ_RS08340 0.21 +1.2 1,537,520 - MMJJ_RS08340 0.22 +1.7 1,537,520 - MMJJ_RS08340 0.22 +0.2 1,537,520 - MMJJ_RS08340 0.22 -1.4 1,537,521 + MMJJ_RS08340 0.22 -1.8 1,537,556 - MMJJ_RS08340 0.28 -1.4 1,537,556 - MMJJ_RS08340 0.28 -1.4 1,537,591 + MMJJ_RS08340 0.34 -1.8
Or see this region's nucleotide sequence