Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08315 and MMJJ_RS08320 are separated by 28 nucleotides MMJJ_RS08320 and MMJJ_RS08325 are separated by 11 nucleotides MMJJ_RS08325 and MMJJ_RS08330 are separated by 149 nucleotides
MMJJ_RS08315: MMJJ_RS08315 - DNA protection protein DPS, at 1,533,409 to 1,533,960
_RS08315
MMJJ_RS08320: MMJJ_RS08320 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,533,989 to 1,534,603
_RS08320
MMJJ_RS08325: MMJJ_RS08325 - glycosyltransferase family 2 protein, at 1,534,615 to 1,535,307
_RS08325
MMJJ_RS08330: MMJJ_RS08330 - SufD family Fe-S cluster assembly protein, at 1,535,457 to 1,536,683
_RS08330
Position (kb)
1534
1535
1536 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1533.631 kb on - strand, within MMJJ_RS08315 at 1533.632 kb on + strand, within MMJJ_RS08315 at 1533.678 kb on - strand, within MMJJ_RS08315 at 1533.681 kb on + strand, within MMJJ_RS08315 at 1533.701 kb on + strand, within MMJJ_RS08315 at 1533.701 kb on + strand, within MMJJ_RS08315 at 1533.729 kb on - strand, within MMJJ_RS08315 at 1533.730 kb on + strand, within MMJJ_RS08315 at 1533.746 kb on - strand, within MMJJ_RS08315 at 1533.771 kb on - strand, within MMJJ_RS08315 at 1533.823 kb on - strand, within MMJJ_RS08315 at 1533.824 kb on + strand, within MMJJ_RS08315 at 1533.824 kb on + strand, within MMJJ_RS08315 at 1533.846 kb on + strand, within MMJJ_RS08315 at 1533.958 kb on - strand at 1533.967 kb on - strand at 1533.968 kb on + strand at 1533.981 kb on + strand at 1533.991 kb on + strand at 1534.003 kb on - strand at 1534.003 kb on - strand at 1534.007 kb on - strand at 1534.007 kb on - strand at 1534.008 kb on + strand at 1534.019 kb on - strand at 1534.019 kb on - strand at 1534.020 kb on + strand at 1534.024 kb on + strand at 1534.024 kb on + strand at 1534.129 kb on - strand, within MMJJ_RS08320 at 1534.140 kb on + strand, within MMJJ_RS08320 at 1534.140 kb on + strand, within MMJJ_RS08320 at 1534.229 kb on - strand, within MMJJ_RS08320 at 1534.325 kb on - strand, within MMJJ_RS08320 at 1534.553 kb on - strand at 1534.756 kb on - strand, within MMJJ_RS08325 at 1534.782 kb on - strand, within MMJJ_RS08325 at 1534.993 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.244 kb on - strand at 1535.268 kb on - strand at 1535.344 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.370 kb on - strand at 1535.370 kb on - strand at 1535.372 kb on - strand at 1535.383 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.389 kb on - strand at 1535.406 kb on - strand at 1535.426 kb on - strand at 1535.427 kb on + strand at 1535.458 kb on - strand at 1535.599 kb on + strand, within MMJJ_RS08330 at 1535.764 kb on + strand, within MMJJ_RS08330 at 1535.783 kb on - strand, within MMJJ_RS08330 at 1535.872 kb on + strand, within MMJJ_RS08330 at 1535.899 kb on + strand, within MMJJ_RS08330 at 1536.153 kb on + strand, within MMJJ_RS08330 at 1536.156 kb on + strand, within MMJJ_RS08330
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,533,631 - MMJJ_RS08315 0.40 -1.6 1,533,632 + MMJJ_RS08315 0.40 +4.6 1,533,678 - MMJJ_RS08315 0.49 +1.1 1,533,681 + MMJJ_RS08315 0.49 +3.0 1,533,701 + MMJJ_RS08315 0.53 -2.4 1,533,701 + MMJJ_RS08315 0.53 +3.0 1,533,729 - MMJJ_RS08315 0.58 +1.0 1,533,730 + MMJJ_RS08315 0.58 -2.6 1,533,746 - MMJJ_RS08315 0.61 -2.1 1,533,771 - MMJJ_RS08315 0.66 -1.3 1,533,823 - MMJJ_RS08315 0.75 -0.6 1,533,824 + MMJJ_RS08315 0.75 -2.9 1,533,824 + MMJJ_RS08315 0.75 -0.4 1,533,846 + MMJJ_RS08315 0.79 -0.6 1,533,958 - -0.7 1,533,967 - +1.0 1,533,968 + +0.1 1,533,981 + +1.6 1,533,991 + +0.5 1,534,003 - -1.5 1,534,003 - +2.6 1,534,007 - +0.4 1,534,007 - +2.7 1,534,008 + +2.2 1,534,019 - -2.0 1,534,019 - -1.1 1,534,020 + -0.8 1,534,024 + +0.3 1,534,024 + +1.2 1,534,129 - MMJJ_RS08320 0.23 -1.8 1,534,140 + MMJJ_RS08320 0.25 -2.1 1,534,140 + MMJJ_RS08320 0.25 -0.8 1,534,229 - MMJJ_RS08320 0.39 -1.8 1,534,325 - MMJJ_RS08320 0.55 -1.8 1,534,553 - +0.2 1,534,756 - MMJJ_RS08325 0.20 -1.4 1,534,782 - MMJJ_RS08325 0.24 -0.8 1,534,993 - MMJJ_RS08325 0.55 +0.6 1,535,200 - MMJJ_RS08325 0.84 -4.1 1,535,200 - MMJJ_RS08325 0.84 +1.2 1,535,244 - +2.0 1,535,268 - -0.8 1,535,344 - +2.6 1,535,357 - -1.4 1,535,357 - +1.2 1,535,357 - -0.4 1,535,370 - -1.5 1,535,370 - +2.6 1,535,372 - +1.9 1,535,383 + -0.2 1,535,386 + -0.6 1,535,386 + -1.6 1,535,386 + +2.9 1,535,386 + -0.0 1,535,389 - +0.9 1,535,406 - +3.0 1,535,426 - +0.6 1,535,427 + -1.8 1,535,458 - +1.5 1,535,599 + MMJJ_RS08330 0.12 +0.2 1,535,764 + MMJJ_RS08330 0.25 +0.2 1,535,783 - MMJJ_RS08330 0.27 -1.1 1,535,872 + MMJJ_RS08330 0.34 -2.1 1,535,899 + MMJJ_RS08330 0.36 -1.8 1,536,153 + MMJJ_RS08330 0.57 +1.2 1,536,156 + MMJJ_RS08330 0.57 -0.8
Or see this region's nucleotide sequence