Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08220 and MMJJ_RS08225 are separated by 190 nucleotides MMJJ_RS08225 and MMJJ_RS08230 are separated by 68 nucleotides MMJJ_RS08230 and MMJJ_RS08235 are separated by 142 nucleotides MMJJ_RS08235 and MMJJ_RS08240 are separated by 80 nucleotides
MMJJ_RS08220: MMJJ_RS08220 - methenyltetrahydromethanopterin cyclohydrolase, at 1,513,835 to 1,514,806
_RS08220
MMJJ_RS08225: MMJJ_RS08225 - peptidylprolyl isomerase, at 1,514,997 to 1,515,449
_RS08225
MMJJ_RS08230: MMJJ_RS08230 - ribose-5-phosphate isomerase RpiA, at 1,515,518 to 1,516,237
_RS08230
MMJJ_RS08235: MMJJ_RS08235 - UPF0147 family protein, at 1,516,380 to 1,516,655
_RS08235
MMJJ_RS08240: MMJJ_RS08240 - L-fuculose phosphate aldolase, at 1,516,736 to 1,517,278
_RS08240
Position (kb)
1515
1516
1517 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1514.681 kb on - strand at 1514.708 kb on + strand, within MMJJ_RS08220 at 1514.725 kb on - strand at 1514.730 kb on + strand at 1514.911 kb on + strand at 1514.911 kb on + strand at 1514.915 kb on - strand at 1514.915 kb on - strand at 1514.916 kb on + strand at 1514.916 kb on + strand at 1514.918 kb on + strand at 1514.918 kb on + strand at 1514.933 kb on - strand at 1514.933 kb on - strand at 1514.933 kb on - strand at 1514.938 kb on - strand at 1514.939 kb on + strand at 1514.939 kb on + strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.945 kb on + strand at 1514.945 kb on + strand at 1514.945 kb on + strand at 1514.947 kb on - strand at 1514.948 kb on + strand at 1514.948 kb on + strand at 1514.948 kb on + strand at 1514.982 kb on - strand at 1514.982 kb on - strand at 1515.014 kb on - strand at 1515.015 kb on + strand at 1515.020 kb on + strand at 1515.020 kb on + strand at 1515.030 kb on - strand at 1515.066 kb on + strand, within MMJJ_RS08225 at 1515.120 kb on - strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.131 kb on + strand, within MMJJ_RS08225 at 1515.131 kb on + strand, within MMJJ_RS08225 at 1515.138 kb on + strand, within MMJJ_RS08225 at 1515.355 kb on - strand, within MMJJ_RS08225 at 1515.362 kb on - strand, within MMJJ_RS08225 at 1515.435 kb on + strand at 1515.436 kb on - strand at 1515.437 kb on + strand at 1515.462 kb on + strand at 1515.470 kb on - strand at 1515.470 kb on - strand at 1515.489 kb on - strand at 1515.498 kb on + strand at 1515.498 kb on + strand at 1515.524 kb on + strand at 1515.550 kb on + strand at 1515.699 kb on + strand, within MMJJ_RS08230 at 1515.700 kb on - strand, within MMJJ_RS08230 at 1515.715 kb on - strand, within MMJJ_RS08230 at 1515.716 kb on + strand, within MMJJ_RS08230 at 1515.802 kb on + strand, within MMJJ_RS08230 at 1515.986 kb on - strand, within MMJJ_RS08230 at 1516.181 kb on + strand at 1516.225 kb on + strand at 1516.250 kb on - strand at 1516.254 kb on - strand at 1516.283 kb on - strand at 1516.283 kb on - strand at 1516.283 kb on - strand at 1516.284 kb on + strand at 1516.284 kb on + strand at 1516.296 kb on + strand at 1516.317 kb on - strand at 1516.317 kb on - strand at 1516.318 kb on + strand at 1516.318 kb on + strand at 1516.327 kb on - strand at 1516.328 kb on + strand at 1516.328 kb on + strand at 1516.328 kb on + strand at 1516.350 kb on - strand at 1516.354 kb on - strand at 1516.355 kb on + strand at 1516.458 kb on + strand, within MMJJ_RS08235 at 1516.511 kb on + strand, within MMJJ_RS08235 at 1516.511 kb on + strand, within MMJJ_RS08235 at 1516.590 kb on + strand, within MMJJ_RS08235 at 1516.653 kb on - strand at 1516.657 kb on - strand at 1516.672 kb on - strand at 1516.714 kb on - strand at 1516.715 kb on + strand at 1516.722 kb on - strand at 1516.754 kb on + strand at 1516.756 kb on - strand at 1516.756 kb on - strand at 1516.757 kb on + strand at 1516.758 kb on - strand at 1516.759 kb on + strand at 1516.759 kb on + strand at 1516.764 kb on + strand at 1516.782 kb on + strand at 1516.782 kb on + strand at 1516.800 kb on - strand, within MMJJ_RS08240 at 1516.800 kb on - strand, within MMJJ_RS08240 at 1516.801 kb on + strand, within MMJJ_RS08240 at 1516.812 kb on + strand, within MMJJ_RS08240 at 1516.813 kb on - strand, within MMJJ_RS08240 at 1516.829 kb on + strand, within MMJJ_RS08240 at 1516.853 kb on + strand, within MMJJ_RS08240 at 1516.915 kb on + strand, within MMJJ_RS08240 at 1516.915 kb on + strand, within MMJJ_RS08240 at 1516.923 kb on - strand, within MMJJ_RS08240 at 1516.923 kb on - strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.928 kb on - strand, within MMJJ_RS08240 at 1516.970 kb on - strand, within MMJJ_RS08240 at 1517.004 kb on - strand, within MMJJ_RS08240 at 1517.004 kb on - strand, within MMJJ_RS08240 at 1517.005 kb on + strand, within MMJJ_RS08240 at 1517.024 kb on - strand, within MMJJ_RS08240 at 1517.024 kb on - strand, within MMJJ_RS08240 at 1517.052 kb on - strand, within MMJJ_RS08240 at 1517.054 kb on - strand, within MMJJ_RS08240 at 1517.072 kb on - strand, within MMJJ_RS08240 at 1517.073 kb on + strand, within MMJJ_RS08240 at 1517.083 kb on - strand, within MMJJ_RS08240 at 1517.097 kb on + strand, within MMJJ_RS08240 at 1517.156 kb on - strand, within MMJJ_RS08240 at 1517.170 kb on - strand, within MMJJ_RS08240 at 1517.171 kb on + strand, within MMJJ_RS08240 at 1517.171 kb on + strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.197 kb on - strand, within MMJJ_RS08240 at 1517.201 kb on - strand, within MMJJ_RS08240 at 1517.201 kb on - strand, within MMJJ_RS08240 at 1517.202 kb on + strand, within MMJJ_RS08240 at 1517.226 kb on - strand at 1517.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,514,681 - +1.2 1,514,708 + MMJJ_RS08220 0.90 -2.0 1,514,725 - -1.6 1,514,730 + -0.8 1,514,911 + -1.7 1,514,911 + -1.3 1,514,915 - -2.1 1,514,915 - -1.1 1,514,916 + +4.2 1,514,916 + -1.1 1,514,918 + -2.5 1,514,918 + -0.3 1,514,933 - +2.1 1,514,933 - -2.0 1,514,933 - -2.6 1,514,938 - +1.2 1,514,939 + -1.1 1,514,939 + -0.4 1,514,940 - +2.3 1,514,940 - -2.4 1,514,940 - -1.0 1,514,940 - -3.4 1,514,940 - +3.5 1,514,940 - -2.9 1,514,941 + +2.6 1,514,941 + +1.9 1,514,941 + +3.9 1,514,941 + +1.0 1,514,941 + -1.9 1,514,941 + +1.8 1,514,941 + -0.4 1,514,941 + +0.5 1,514,941 + -2.2 1,514,942 - +2.6 1,514,942 - -1.1 1,514,942 - +0.6 1,514,942 - -0.7 1,514,942 - +1.1 1,514,942 - +2.9 1,514,942 - +0.2 1,514,942 - -1.4 1,514,942 - +1.1 1,514,942 - +0.6 1,514,942 - +4.5 1,514,942 - +0.2 1,514,942 - -0.6 1,514,943 + -0.0 1,514,943 + -0.6 1,514,943 + -0.4 1,514,943 + +1.0 1,514,943 + -1.0 1,514,943 + -0.8 1,514,943 + +5.0 1,514,943 + +3.7 1,514,944 - +2.3 1,514,944 - +1.2 1,514,944 - -1.1 1,514,944 - -1.1 1,514,944 - -1.3 1,514,944 - -1.1 1,514,944 - +0.8 1,514,945 + -1.4 1,514,945 + -1.8 1,514,945 + -0.8 1,514,947 - -0.4 1,514,948 + -2.2 1,514,948 + -1.4 1,514,948 + -1.5 1,514,982 - -0.6 1,514,982 - -1.5 1,515,014 - +4.0 1,515,015 + -1.8 1,515,020 + -0.8 1,515,020 + +1.9 1,515,030 - -1.8 1,515,066 + MMJJ_RS08225 0.15 -2.0 1,515,120 - MMJJ_RS08225 0.27 -2.7 1,515,121 + MMJJ_RS08225 0.27 +0.2 1,515,121 + MMJJ_RS08225 0.27 +0.5 1,515,121 + MMJJ_RS08225 0.27 +4.6 1,515,131 + MMJJ_RS08225 0.30 -2.0 1,515,131 + MMJJ_RS08225 0.30 +1.2 1,515,138 + MMJJ_RS08225 0.31 +3.6 1,515,355 - MMJJ_RS08225 0.79 +0.6 1,515,362 - MMJJ_RS08225 0.81 -1.6 1,515,435 + -1.1 1,515,436 - +1.0 1,515,437 + -1.0 1,515,462 + +1.2 1,515,470 - -2.1 1,515,470 - -0.4 1,515,489 - -2.2 1,515,498 + -0.4 1,515,498 + +1.4 1,515,524 + -1.1 1,515,550 + +1.2 1,515,699 + MMJJ_RS08230 0.25 -1.6 1,515,700 - MMJJ_RS08230 0.25 -1.1 1,515,715 - MMJJ_RS08230 0.27 -2.1 1,515,716 + MMJJ_RS08230 0.28 -0.4 1,515,802 + MMJJ_RS08230 0.39 +0.2 1,515,986 - MMJJ_RS08230 0.65 -0.4 1,516,181 + +1.2 1,516,225 + +0.5 1,516,250 - -0.9 1,516,254 - -1.5 1,516,283 - +4.0 1,516,283 - -2.6 1,516,283 - -2.1 1,516,284 + -0.6 1,516,284 + +1.0 1,516,296 + +0.7 1,516,317 - -1.1 1,516,317 - -1.4 1,516,318 + +0.1 1,516,318 + +6.5 1,516,327 - +0.2 1,516,328 + -0.8 1,516,328 + -2.2 1,516,328 + -1.0 1,516,350 - +2.0 1,516,354 - +0.2 1,516,355 + +1.9 1,516,458 + MMJJ_RS08235 0.28 -2.3 1,516,511 + MMJJ_RS08235 0.47 -2.1 1,516,511 + MMJJ_RS08235 0.47 +0.2 1,516,590 + MMJJ_RS08235 0.76 +2.2 1,516,653 - +1.1 1,516,657 - -0.2 1,516,672 - -2.8 1,516,714 - +1.8 1,516,715 + -3.7 1,516,722 - -2.0 1,516,754 + -0.8 1,516,756 - -3.9 1,516,756 - -0.3 1,516,757 + +2.5 1,516,758 - +0.2 1,516,759 + +3.8 1,516,759 + +1.8 1,516,764 + -1.5 1,516,782 + -0.5 1,516,782 + +1.1 1,516,800 - MMJJ_RS08240 0.12 -1.9 1,516,800 - MMJJ_RS08240 0.12 +1.8 1,516,801 + MMJJ_RS08240 0.12 +1.1 1,516,812 + MMJJ_RS08240 0.14 +1.7 1,516,813 - MMJJ_RS08240 0.14 -1.6 1,516,829 + MMJJ_RS08240 0.17 +1.7 1,516,853 + MMJJ_RS08240 0.22 +0.5 1,516,915 + MMJJ_RS08240 0.33 -0.4 1,516,915 + MMJJ_RS08240 0.33 +0.4 1,516,923 - MMJJ_RS08240 0.34 +0.5 1,516,923 - MMJJ_RS08240 0.34 +0.6 1,516,924 + MMJJ_RS08240 0.35 -0.5 1,516,924 + MMJJ_RS08240 0.35 -3.9 1,516,924 + MMJJ_RS08240 0.35 +1.0 1,516,924 + MMJJ_RS08240 0.35 -2.0 1,516,924 + MMJJ_RS08240 0.35 +3.1 1,516,924 + MMJJ_RS08240 0.35 -0.9 1,516,928 - MMJJ_RS08240 0.35 +3.2 1,516,970 - MMJJ_RS08240 0.43 +3.0 1,517,004 - MMJJ_RS08240 0.49 +1.4 1,517,004 - MMJJ_RS08240 0.49 -0.5 1,517,005 + MMJJ_RS08240 0.50 -0.2 1,517,024 - MMJJ_RS08240 0.53 +1.0 1,517,024 - MMJJ_RS08240 0.53 -0.8 1,517,052 - MMJJ_RS08240 0.58 +0.5 1,517,054 - MMJJ_RS08240 0.59 -1.8 1,517,072 - MMJJ_RS08240 0.62 +2.0 1,517,073 + MMJJ_RS08240 0.62 +3.4 1,517,083 - MMJJ_RS08240 0.64 +1.5 1,517,097 + MMJJ_RS08240 0.66 +1.6 1,517,156 - MMJJ_RS08240 0.77 +0.4 1,517,170 - MMJJ_RS08240 0.80 +0.7 1,517,171 + MMJJ_RS08240 0.80 +2.4 1,517,171 + MMJJ_RS08240 0.80 -1.4 1,517,173 - MMJJ_RS08240 0.80 +1.5 1,517,173 - MMJJ_RS08240 0.80 +0.3 1,517,173 - MMJJ_RS08240 0.80 -1.4 1,517,175 - MMJJ_RS08240 0.81 +0.5 1,517,175 - MMJJ_RS08240 0.81 -2.0 1,517,175 - MMJJ_RS08240 0.81 +2.8 1,517,175 - MMJJ_RS08240 0.81 -2.6 1,517,176 + MMJJ_RS08240 0.81 -2.1 1,517,176 + MMJJ_RS08240 0.81 -1.5 1,517,176 + MMJJ_RS08240 0.81 +0.7 1,517,176 + MMJJ_RS08240 0.81 -1.0 1,517,176 + MMJJ_RS08240 0.81 -0.9 1,517,197 - MMJJ_RS08240 0.85 -1.6 1,517,201 - MMJJ_RS08240 0.86 -1.7 1,517,201 - MMJJ_RS08240 0.86 +0.8 1,517,202 + MMJJ_RS08240 0.86 -2.1 1,517,226 - +1.8 1,517,226 - +0.2
Or see this region's nucleotide sequence