Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07970 and MMJJ_RS07975 are separated by 27 nucleotides MMJJ_RS07975 and MMJJ_RS07980 overlap by 4 nucleotides
MMJJ_RS07970: MMJJ_RS07970 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,461,221 to 1,462,363
_RS07970
MMJJ_RS07975: MMJJ_RS07975 - ATP-dependent sacrificial sulfur transferase LarE, at 1,462,391 to 1,463,167
_RS07975
MMJJ_RS07980: MMJJ_RS07980 - biotin synthase BioB, at 1,463,164 to 1,464,147
_RS07980
Position (kb)
1462
1463
1464 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1462.053 kb on + strand, within MMJJ_RS07970 at 1462.093 kb on + strand, within MMJJ_RS07970 at 1462.246 kb on + strand, within MMJJ_RS07970 at 1462.299 kb on + strand at 1462.327 kb on - strand at 1462.345 kb on - strand at 1462.364 kb on + strand at 1462.388 kb on - strand at 1462.392 kb on - strand at 1462.392 kb on - strand at 1462.393 kb on + strand at 1462.412 kb on - strand at 1462.412 kb on - strand at 1462.413 kb on + strand at 1462.431 kb on + strand at 1462.447 kb on - strand at 1462.451 kb on - strand at 1462.451 kb on - strand at 1462.454 kb on - strand at 1462.454 kb on - strand at 1462.455 kb on + strand at 1462.499 kb on + strand, within MMJJ_RS07975 at 1462.510 kb on - strand, within MMJJ_RS07975 at 1462.545 kb on - strand, within MMJJ_RS07975 at 1462.572 kb on + strand, within MMJJ_RS07975 at 1462.603 kb on - strand, within MMJJ_RS07975 at 1462.604 kb on + strand, within MMJJ_RS07975 at 1462.651 kb on - strand, within MMJJ_RS07975 at 1462.652 kb on + strand, within MMJJ_RS07975 at 1462.680 kb on - strand, within MMJJ_RS07975 at 1462.681 kb on + strand, within MMJJ_RS07975 at 1462.754 kb on + strand, within MMJJ_RS07975 at 1462.764 kb on + strand, within MMJJ_RS07975 at 1462.784 kb on - strand, within MMJJ_RS07975 at 1462.786 kb on - strand, within MMJJ_RS07975 at 1462.821 kb on - strand, within MMJJ_RS07975 at 1462.821 kb on - strand, within MMJJ_RS07975 at 1462.846 kb on - strand, within MMJJ_RS07975 at 1463.059 kb on - strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.234 kb on - strand at 1463.352 kb on - strand, within MMJJ_RS07980 at 1463.353 kb on + strand, within MMJJ_RS07980 at 1463.356 kb on - strand, within MMJJ_RS07980 at 1463.392 kb on - strand, within MMJJ_RS07980 at 1463.393 kb on + strand, within MMJJ_RS07980 at 1463.394 kb on - strand, within MMJJ_RS07980 at 1463.394 kb on - strand, within MMJJ_RS07980 at 1463.394 kb on - strand, within MMJJ_RS07980 at 1463.395 kb on + strand, within MMJJ_RS07980 at 1463.407 kb on - strand, within MMJJ_RS07980 at 1463.407 kb on - strand, within MMJJ_RS07980 at 1463.413 kb on + strand, within MMJJ_RS07980 at 1463.443 kb on - strand, within MMJJ_RS07980 at 1463.446 kb on - strand, within MMJJ_RS07980 at 1463.452 kb on - strand, within MMJJ_RS07980 at 1463.453 kb on + strand, within MMJJ_RS07980 at 1463.483 kb on + strand, within MMJJ_RS07980 at 1463.491 kb on + strand, within MMJJ_RS07980 at 1463.514 kb on - strand, within MMJJ_RS07980 at 1463.514 kb on - strand at 1463.515 kb on + strand, within MMJJ_RS07980 at 1463.613 kb on - strand, within MMJJ_RS07980 at 1463.619 kb on - strand, within MMJJ_RS07980 at 1463.933 kb on + strand, within MMJJ_RS07980 at 1463.969 kb on + strand, within MMJJ_RS07980 at 1463.991 kb on - strand, within MMJJ_RS07980 at 1463.991 kb on - strand, within MMJJ_RS07980 at 1463.998 kb on + strand, within MMJJ_RS07980 at 1464.013 kb on - strand, within MMJJ_RS07980 at 1464.014 kb on + strand, within MMJJ_RS07980 at 1464.020 kb on - strand, within MMJJ_RS07980 at 1464.020 kb on - strand, within MMJJ_RS07980 at 1464.021 kb on + strand, within MMJJ_RS07980 at 1464.025 kb on - strand, within MMJJ_RS07980 at 1464.025 kb on - strand, within MMJJ_RS07980 at 1464.026 kb on + strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.028 kb on + strand, within MMJJ_RS07980 at 1464.028 kb on + strand, within MMJJ_RS07980 at 1464.028 kb on + strand, within MMJJ_RS07980 at 1464.043 kb on - strand, within MMJJ_RS07980 at 1464.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,462,053 + MMJJ_RS07970 0.73 -2.6 1,462,093 + MMJJ_RS07970 0.76 -0.8 1,462,246 + MMJJ_RS07970 0.90 -1.6 1,462,299 + +0.2 1,462,327 - -1.4 1,462,345 - -1.4 1,462,364 + -1.4 1,462,388 - +1.9 1,462,392 - -2.5 1,462,392 - -0.8 1,462,393 + -0.7 1,462,412 - +0.7 1,462,412 - +0.6 1,462,413 + -1.4 1,462,431 + -2.1 1,462,447 - -1.2 1,462,451 - -1.8 1,462,451 - +0.2 1,462,454 - -1.5 1,462,454 - -2.9 1,462,455 + -2.2 1,462,499 + MMJJ_RS07975 0.14 -3.1 1,462,510 - MMJJ_RS07975 0.15 -3.7 1,462,545 - MMJJ_RS07975 0.20 -2.4 1,462,572 + MMJJ_RS07975 0.23 -0.9 1,462,603 - MMJJ_RS07975 0.27 -2.1 1,462,604 + MMJJ_RS07975 0.27 +0.2 1,462,651 - MMJJ_RS07975 0.33 -0.8 1,462,652 + MMJJ_RS07975 0.34 -2.5 1,462,680 - MMJJ_RS07975 0.37 +0.2 1,462,681 + MMJJ_RS07975 0.37 -0.6 1,462,754 + MMJJ_RS07975 0.47 -2.9 1,462,764 + MMJJ_RS07975 0.48 -1.2 1,462,784 - MMJJ_RS07975 0.51 -1.5 1,462,786 - MMJJ_RS07975 0.51 -4.0 1,462,821 - MMJJ_RS07975 0.55 -1.3 1,462,821 - MMJJ_RS07975 0.55 -3.4 1,462,846 - MMJJ_RS07975 0.59 -2.9 1,463,059 - MMJJ_RS07975 0.86 -1.3 1,463,060 + MMJJ_RS07975 0.86 -2.4 1,463,060 + MMJJ_RS07975 0.86 -0.3 1,463,060 + MMJJ_RS07975 0.86 -2.9 1,463,234 - -1.3 1,463,352 - MMJJ_RS07980 0.19 -0.5 1,463,353 + MMJJ_RS07980 0.19 -2.0 1,463,356 - MMJJ_RS07980 0.20 +1.2 1,463,392 - MMJJ_RS07980 0.23 -1.1 1,463,393 + MMJJ_RS07980 0.23 -1.1 1,463,394 - MMJJ_RS07980 0.23 -1.8 1,463,394 - MMJJ_RS07980 0.23 +0.2 1,463,394 - MMJJ_RS07980 0.23 -2.0 1,463,395 + MMJJ_RS07980 0.23 -0.4 1,463,407 - MMJJ_RS07980 0.25 -1.4 1,463,407 - MMJJ_RS07980 0.25 -0.1 1,463,413 + MMJJ_RS07980 0.25 -2.5 1,463,443 - MMJJ_RS07980 0.28 -0.6 1,463,446 - MMJJ_RS07980 0.29 +1.2 1,463,452 - MMJJ_RS07980 0.29 -2.1 1,463,453 + MMJJ_RS07980 0.29 -2.3 1,463,483 + MMJJ_RS07980 0.32 -3.5 1,463,491 + MMJJ_RS07980 0.33 -2.2 1,463,514 - MMJJ_RS07980 0.36 -0.6 1,463,514 - +1.2 1,463,515 + MMJJ_RS07980 0.36 -0.4 1,463,613 - MMJJ_RS07980 0.46 -1.7 1,463,619 - MMJJ_RS07980 0.46 -2.0 1,463,933 + MMJJ_RS07980 0.78 -0.4 1,463,969 + MMJJ_RS07980 0.82 -0.8 1,463,991 - MMJJ_RS07980 0.84 -2.4 1,463,991 - MMJJ_RS07980 0.84 -1.1 1,463,998 + MMJJ_RS07980 0.85 -4.0 1,464,013 - MMJJ_RS07980 0.86 -0.8 1,464,014 + MMJJ_RS07980 0.86 -1.8 1,464,020 - MMJJ_RS07980 0.87 +0.2 1,464,020 - MMJJ_RS07980 0.87 -0.0 1,464,021 + MMJJ_RS07980 0.87 -0.8 1,464,025 - MMJJ_RS07980 0.88 -0.5 1,464,025 - MMJJ_RS07980 0.88 -2.2 1,464,026 + MMJJ_RS07980 0.88 +0.5 1,464,027 - MMJJ_RS07980 0.88 -1.7 1,464,027 - MMJJ_RS07980 0.88 -0.8 1,464,027 - MMJJ_RS07980 0.88 -1.4 1,464,027 - MMJJ_RS07980 0.88 -1.5 1,464,028 + MMJJ_RS07980 0.88 -1.0 1,464,028 + MMJJ_RS07980 0.88 -1.1 1,464,028 + MMJJ_RS07980 0.88 -0.4 1,464,043 - MMJJ_RS07980 0.89 -0.3 1,464,067 - -0.8
Or see this region's nucleotide sequence