Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07500 and MMJJ_RS07505 are separated by 31 nucleotides MMJJ_RS07505 and MMJJ_RS07510 are separated by 37 nucleotides MMJJ_RS07510 and MMJJ_RS07515 are separated by 37 nucleotides MMJJ_RS07515 and MMJJ_RS07520 are separated by 38 nucleotides MMJJ_RS07520 and MMJJ_RS07525 are separated by 13 nucleotides
MMJJ_RS07500: MMJJ_RS07500 - hypothetical protein, at 1,377,348 to 1,378,283
_RS07500
MMJJ_RS07505: MMJJ_RS07505 - DNA-directed RNA polymerase subunit K, at 1,378,315 to 1,378,488
_RS07505
MMJJ_RS07510: MMJJ_RS07510 - tRNA-Pro, at 1,378,526 to 1,378,599
_RS07510
MMJJ_RS07515: MMJJ_RS07515 - DNA-directed RNA polymerase subunit N, at 1,378,637 to 1,378,843
_RS07515
MMJJ_RS07520: MMJJ_RS07520 - 30S ribosomal protein S9, at 1,378,882 to 1,379,286
_RS07520
MMJJ_RS07525: MMJJ_RS07525 - 50S ribosomal protein L13, at 1,379,300 to 1,379,713
_RS07525
Position (kb)
1378
1379 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1377.328 kb on + strand at 1377.329 kb on - strand at 1377.330 kb on + strand at 1377.347 kb on + strand at 1377.359 kb on - strand at 1377.359 kb on - strand at 1377.360 kb on + strand at 1377.460 kb on + strand, within MMJJ_RS07500 at 1377.520 kb on - strand, within MMJJ_RS07500 at 1377.527 kb on - strand, within MMJJ_RS07500 at 1377.528 kb on + strand, within MMJJ_RS07500 at 1377.529 kb on - strand, within MMJJ_RS07500 at 1377.529 kb on - strand, within MMJJ_RS07500 at 1377.530 kb on + strand, within MMJJ_RS07500 at 1377.530 kb on + strand, within MMJJ_RS07500 at 1377.562 kb on - strand, within MMJJ_RS07500 at 1377.563 kb on + strand, within MMJJ_RS07500 at 1377.633 kb on - strand, within MMJJ_RS07500 at 1377.648 kb on - strand, within MMJJ_RS07500 at 1377.649 kb on + strand, within MMJJ_RS07500 at 1377.649 kb on + strand, within MMJJ_RS07500 at 1377.652 kb on - strand, within MMJJ_RS07500 at 1377.653 kb on + strand, within MMJJ_RS07500 at 1377.703 kb on + strand, within MMJJ_RS07500 at 1377.705 kb on - strand, within MMJJ_RS07500 at 1377.731 kb on - strand, within MMJJ_RS07500 at 1377.785 kb on - strand, within MMJJ_RS07500 at 1377.786 kb on + strand, within MMJJ_RS07500 at 1377.787 kb on - strand, within MMJJ_RS07500 at 1377.797 kb on + strand, within MMJJ_RS07500 at 1377.801 kb on + strand, within MMJJ_RS07500 at 1377.801 kb on + strand, within MMJJ_RS07500 at 1377.815 kb on + strand, within MMJJ_RS07500 at 1377.815 kb on + strand, within MMJJ_RS07500 at 1377.838 kb on + strand, within MMJJ_RS07500 at 1377.842 kb on - strand, within MMJJ_RS07500 at 1377.851 kb on - strand, within MMJJ_RS07500 at 1377.851 kb on - strand, within MMJJ_RS07500 at 1377.851 kb on - strand, within MMJJ_RS07500 at 1377.852 kb on + strand, within MMJJ_RS07500 at 1377.852 kb on + strand, within MMJJ_RS07500 at 1377.852 kb on + strand, within MMJJ_RS07500 at 1377.852 kb on + strand, within MMJJ_RS07500 at 1377.852 kb on + strand, within MMJJ_RS07500 at 1377.856 kb on + strand, within MMJJ_RS07500 at 1377.955 kb on + strand, within MMJJ_RS07500 at 1378.017 kb on - strand, within MMJJ_RS07500 at 1378.018 kb on + strand, within MMJJ_RS07500 at 1378.050 kb on - strand, within MMJJ_RS07500 at 1378.065 kb on - strand, within MMJJ_RS07500 at 1378.065 kb on - strand at 1378.065 kb on - strand, within MMJJ_RS07500 at 1378.069 kb on + strand, within MMJJ_RS07500 at 1378.083 kb on + strand, within MMJJ_RS07500 at 1378.093 kb on - strand, within MMJJ_RS07500 at 1378.094 kb on + strand, within MMJJ_RS07500 at 1378.126 kb on + strand, within MMJJ_RS07500 at 1378.146 kb on - strand, within MMJJ_RS07500 at 1378.148 kb on - strand, within MMJJ_RS07500 at 1378.149 kb on + strand, within MMJJ_RS07500 at 1378.171 kb on + strand, within MMJJ_RS07500 at 1378.235 kb on - strand at 1378.236 kb on + strand at 1378.308 kb on - strand at 1378.312 kb on + strand at 1378.343 kb on + strand, within MMJJ_RS07505 at 1378.607 kb on - strand at 1378.622 kb on - strand at 1378.634 kb on - strand at 1378.854 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,377,328 + +3.9 1,377,329 - -0.0 1,377,330 + +3.6 1,377,347 + +0.8 1,377,359 - +1.2 1,377,359 - +1.7 1,377,360 + +3.4 1,377,460 + MMJJ_RS07500 0.12 +0.5 1,377,520 - MMJJ_RS07500 0.18 -1.7 1,377,527 - MMJJ_RS07500 0.19 +0.3 1,377,528 + MMJJ_RS07500 0.19 -2.6 1,377,529 - MMJJ_RS07500 0.19 -3.7 1,377,529 - MMJJ_RS07500 0.19 -2.1 1,377,530 + MMJJ_RS07500 0.19 -0.6 1,377,530 + MMJJ_RS07500 0.19 -0.1 1,377,562 - MMJJ_RS07500 0.23 -3.8 1,377,563 + MMJJ_RS07500 0.23 -0.7 1,377,633 - MMJJ_RS07500 0.30 -1.1 1,377,648 - MMJJ_RS07500 0.32 -0.7 1,377,649 + MMJJ_RS07500 0.32 +2.7 1,377,649 + MMJJ_RS07500 0.32 +1.2 1,377,652 - MMJJ_RS07500 0.32 -1.0 1,377,653 + MMJJ_RS07500 0.33 +0.6 1,377,703 + MMJJ_RS07500 0.38 -2.6 1,377,705 - MMJJ_RS07500 0.38 -2.2 1,377,731 - MMJJ_RS07500 0.41 +1.9 1,377,785 - MMJJ_RS07500 0.47 -1.3 1,377,786 + MMJJ_RS07500 0.47 -2.6 1,377,787 - MMJJ_RS07500 0.47 -1.1 1,377,797 + MMJJ_RS07500 0.48 -2.4 1,377,801 + MMJJ_RS07500 0.48 -0.4 1,377,801 + MMJJ_RS07500 0.48 -3.7 1,377,815 + MMJJ_RS07500 0.50 -2.9 1,377,815 + MMJJ_RS07500 0.50 +3.4 1,377,838 + MMJJ_RS07500 0.52 -1.9 1,377,842 - MMJJ_RS07500 0.53 -2.4 1,377,851 - MMJJ_RS07500 0.54 +1.0 1,377,851 - MMJJ_RS07500 0.54 -1.5 1,377,851 - MMJJ_RS07500 0.54 -1.2 1,377,852 + MMJJ_RS07500 0.54 +0.4 1,377,852 + MMJJ_RS07500 0.54 -0.1 1,377,852 + MMJJ_RS07500 0.54 -1.1 1,377,852 + MMJJ_RS07500 0.54 -0.3 1,377,852 + MMJJ_RS07500 0.54 +1.2 1,377,856 + MMJJ_RS07500 0.54 -1.3 1,377,955 + MMJJ_RS07500 0.65 -0.3 1,378,017 - MMJJ_RS07500 0.71 +0.5 1,378,018 + MMJJ_RS07500 0.72 -2.9 1,378,050 - MMJJ_RS07500 0.75 -2.3 1,378,065 - MMJJ_RS07500 0.77 -1.6 1,378,065 - +0.3 1,378,065 - MMJJ_RS07500 0.77 +1.2 1,378,069 + MMJJ_RS07500 0.77 +0.8 1,378,083 + MMJJ_RS07500 0.79 -1.2 1,378,093 - MMJJ_RS07500 0.80 -2.3 1,378,094 + MMJJ_RS07500 0.80 -3.4 1,378,126 + MMJJ_RS07500 0.83 -0.7 1,378,146 - MMJJ_RS07500 0.85 +2.3 1,378,148 - MMJJ_RS07500 0.85 -0.7 1,378,149 + MMJJ_RS07500 0.86 -3.8 1,378,171 + MMJJ_RS07500 0.88 -1.7 1,378,235 - -3.2 1,378,236 + -0.3 1,378,308 - -2.0 1,378,312 + +0.2 1,378,343 + MMJJ_RS07505 0.16 -2.4 1,378,607 - +0.3 1,378,622 - -1.9 1,378,634 - +1.3 1,378,854 - -1.9
Or see this region's nucleotide sequence