Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07405 and MMJJ_RS07410 are separated by 129 nucleotides MMJJ_RS07410 and MMJJ_RS07415 are separated by 16 nucleotides MMJJ_RS07415 and MMJJ_RS07420 are separated by 3 nucleotides MMJJ_RS07420 and MMJJ_RS07425 are separated by 9 nucleotides
MMJJ_RS07405: MMJJ_RS07405 - histone family protein, at 1,360,406 to 1,360,606
_RS07405
MMJJ_RS07410: MMJJ_RS07410 - methanogenesis marker protein Mmp4/MtxX, at 1,360,736 to 1,361,470
_RS07410
MMJJ_RS07415: MMJJ_RS07415 - polyprenyl diphosphate synthase, at 1,361,487 to 1,362,281
_RS07415
MMJJ_RS07420: MMJJ_RS07420 - protease complex subunit PrcB family protein, at 1,362,285 to 1,362,752
_RS07420
MMJJ_RS07425: MMJJ_RS07425 - MBL fold metallo-hydrolase, at 1,362,762 to 1,363,562
_RS07425
Position (kb)
1361
1362
1363 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1360.504 kb on + strand, within MMJJ_RS07405 at 1360.524 kb on - strand, within MMJJ_RS07405 at 1360.546 kb on + strand, within MMJJ_RS07405 at 1360.571 kb on + strand, within MMJJ_RS07405 at 1360.573 kb on - strand, within MMJJ_RS07405 at 1360.574 kb on + strand, within MMJJ_RS07405 at 1360.593 kb on - strand at 1360.604 kb on - strand at 1360.605 kb on + strand at 1360.613 kb on - strand at 1360.638 kb on - strand at 1360.638 kb on - strand at 1360.638 kb on - strand at 1360.638 kb on - strand at 1360.669 kb on - strand at 1360.669 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.674 kb on + strand at 1360.674 kb on + strand at 1360.684 kb on - strand at 1360.685 kb on + strand at 1360.710 kb on + strand at 1360.715 kb on - strand at 1360.716 kb on + strand at 1360.724 kb on + strand at 1360.739 kb on - strand at 1360.739 kb on - strand at 1360.739 kb on - strand at 1361.007 kb on - strand, within MMJJ_RS07410 at 1361.052 kb on - strand at 1361.056 kb on - strand, within MMJJ_RS07410 at 1361.057 kb on + strand at 1361.059 kb on + strand, within MMJJ_RS07410 at 1361.062 kb on - strand, within MMJJ_RS07410 at 1361.082 kb on - strand, within MMJJ_RS07410 at 1361.203 kb on - strand, within MMJJ_RS07410 at 1361.216 kb on + strand, within MMJJ_RS07410 at 1361.396 kb on - strand, within MMJJ_RS07410 at 1361.396 kb on - strand, within MMJJ_RS07410 at 1361.396 kb on - strand, within MMJJ_RS07410 at 1361.397 kb on + strand at 1361.397 kb on + strand at 1361.400 kb on - strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.404 kb on - strand at 1361.404 kb on - strand at 1361.405 kb on + strand at 1361.405 kb on + strand at 1361.602 kb on - strand at 1361.893 kb on + strand, within MMJJ_RS07415 at 1361.893 kb on + strand, within MMJJ_RS07415 at 1361.909 kb on - strand at 1361.913 kb on - strand, within MMJJ_RS07415 at 1361.924 kb on - strand, within MMJJ_RS07415 at 1361.990 kb on + strand, within MMJJ_RS07415 at 1362.052 kb on - strand, within MMJJ_RS07415 at 1362.202 kb on + strand at 1362.300 kb on + strand at 1362.304 kb on - strand at 1362.307 kb on - strand at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.351 kb on + strand, within MMJJ_RS07420 at 1362.351 kb on + strand, within MMJJ_RS07420 at 1362.353 kb on - strand, within MMJJ_RS07420 at 1362.383 kb on + strand, within MMJJ_RS07420 at 1362.390 kb on + strand, within MMJJ_RS07420 at 1362.419 kb on + strand, within MMJJ_RS07420 at 1362.425 kb on + strand, within MMJJ_RS07420 at 1362.449 kb on - strand, within MMJJ_RS07420 at 1362.449 kb on - strand, within MMJJ_RS07420 at 1362.449 kb on - strand, within MMJJ_RS07420 at 1362.450 kb on + strand, within MMJJ_RS07420 at 1362.477 kb on + strand, within MMJJ_RS07420 at 1362.496 kb on - strand, within MMJJ_RS07420 at 1362.496 kb on - strand, within MMJJ_RS07420 at 1362.496 kb on - strand, within MMJJ_RS07420 at 1362.496 kb on - strand, within MMJJ_RS07420 at 1362.497 kb on + strand, within MMJJ_RS07420 at 1362.498 kb on - strand, within MMJJ_RS07420 at 1362.498 kb on - strand, within MMJJ_RS07420 at 1362.498 kb on - strand, within MMJJ_RS07420 at 1362.511 kb on + strand, within MMJJ_RS07420 at 1362.721 kb on + strand at 1362.809 kb on - strand at 1362.810 kb on + strand at 1362.810 kb on + strand at 1362.811 kb on - strand at 1362.812 kb on + strand at 1362.812 kb on + strand at 1362.839 kb on - strand at 1362.863 kb on - strand, within MMJJ_RS07425 at 1363.093 kb on + strand, within MMJJ_RS07425 at 1363.145 kb on + strand, within MMJJ_RS07425 at 1363.145 kb on + strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.165 kb on - strand, within MMJJ_RS07425 at 1363.166 kb on + strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.204 kb on - strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.212 kb on + strand, within MMJJ_RS07425 at 1363.234 kb on - strand, within MMJJ_RS07425 at 1363.234 kb on - strand, within MMJJ_RS07425 at 1363.244 kb on - strand, within MMJJ_RS07425 at 1363.260 kb on - strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.261 kb on + strand, within MMJJ_RS07425 at 1363.274 kb on + strand, within MMJJ_RS07425
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,360,504 + MMJJ_RS07405 0.49 -2.7 1,360,524 - MMJJ_RS07405 0.59 -0.9 1,360,546 + MMJJ_RS07405 0.70 +2.2 1,360,571 + MMJJ_RS07405 0.82 +0.5 1,360,573 - MMJJ_RS07405 0.83 +2.3 1,360,574 + MMJJ_RS07405 0.84 -1.7 1,360,593 - -0.4 1,360,604 - -0.4 1,360,605 + +3.2 1,360,613 - +1.8 1,360,638 - +1.8 1,360,638 - -3.3 1,360,638 - +2.6 1,360,638 - -0.8 1,360,669 - -0.6 1,360,669 - -2.0 1,360,671 - -1.7 1,360,671 - -2.3 1,360,671 - +0.0 1,360,671 - -2.2 1,360,671 - +0.4 1,360,671 - +1.7 1,360,671 - -1.0 1,360,671 - +0.3 1,360,671 - -2.1 1,360,672 + -2.7 1,360,672 + -1.2 1,360,672 + +1.0 1,360,672 + -1.5 1,360,672 + -0.1 1,360,672 + +1.0 1,360,672 + -1.2 1,360,672 + +1.2 1,360,673 - -0.5 1,360,673 - -1.2 1,360,673 - -0.5 1,360,673 - +5.5 1,360,673 - +1.6 1,360,673 - -0.4 1,360,673 - +3.6 1,360,674 + -1.3 1,360,674 + -0.5 1,360,684 - -2.6 1,360,685 + +0.7 1,360,710 + +0.6 1,360,715 - +0.1 1,360,716 + -0.7 1,360,724 + -0.2 1,360,739 - -1.7 1,360,739 - -0.7 1,360,739 - +1.3 1,361,007 - MMJJ_RS07410 0.37 -2.0 1,361,052 - -0.2 1,361,056 - MMJJ_RS07410 0.44 +1.3 1,361,057 + +1.3 1,361,059 + MMJJ_RS07410 0.44 -0.2 1,361,062 - MMJJ_RS07410 0.44 -0.1 1,361,082 - MMJJ_RS07410 0.47 -0.5 1,361,203 - MMJJ_RS07410 0.64 -0.7 1,361,216 + MMJJ_RS07410 0.65 -1.2 1,361,396 - MMJJ_RS07410 0.90 +0.3 1,361,396 - MMJJ_RS07410 0.90 +0.3 1,361,396 - MMJJ_RS07410 0.90 -1.5 1,361,397 + +1.3 1,361,397 + -1.2 1,361,400 - +0.3 1,361,401 + -0.7 1,361,401 + -1.0 1,361,401 + -0.2 1,361,401 + -0.7 1,361,401 + -0.7 1,361,404 - -1.0 1,361,404 - +1.3 1,361,405 + +0.3 1,361,405 + -0.7 1,361,602 - -0.2 1,361,893 + MMJJ_RS07415 0.51 -0.7 1,361,893 + MMJJ_RS07415 0.51 +0.9 1,361,909 - +1.3 1,361,913 - MMJJ_RS07415 0.54 -1.2 1,361,924 - MMJJ_RS07415 0.55 -1.0 1,361,990 + MMJJ_RS07415 0.63 -1.0 1,362,052 - MMJJ_RS07415 0.71 -1.0 1,362,202 + +0.7 1,362,300 + -2.8 1,362,304 - +2.4 1,362,307 - -1.4 1,362,339 + MMJJ_RS07420 0.12 -2.6 1,362,339 + MMJJ_RS07420 0.12 +0.6 1,362,339 + MMJJ_RS07420 0.12 +1.2 1,362,339 + MMJJ_RS07420 0.12 +0.1 1,362,339 + MMJJ_RS07420 0.12 -0.1 1,362,350 - MMJJ_RS07420 0.14 -1.7 1,362,350 - MMJJ_RS07420 0.14 -1.4 1,362,350 - MMJJ_RS07420 0.14 +0.6 1,362,350 - MMJJ_RS07420 0.14 +1.2 1,362,351 + MMJJ_RS07420 0.14 -2.3 1,362,351 + MMJJ_RS07420 0.14 +0.9 1,362,353 - MMJJ_RS07420 0.15 +0.8 1,362,383 + MMJJ_RS07420 0.21 -0.1 1,362,390 + MMJJ_RS07420 0.22 +2.1 1,362,419 + MMJJ_RS07420 0.29 +3.1 1,362,425 + MMJJ_RS07420 0.30 -2.7 1,362,449 - MMJJ_RS07420 0.35 +1.1 1,362,449 - MMJJ_RS07420 0.35 -0.1 1,362,449 - MMJJ_RS07420 0.35 -1.0 1,362,450 + MMJJ_RS07420 0.35 +1.7 1,362,477 + MMJJ_RS07420 0.41 -0.1 1,362,496 - MMJJ_RS07420 0.45 -3.5 1,362,496 - MMJJ_RS07420 0.45 +1.8 1,362,496 - MMJJ_RS07420 0.45 +1.3 1,362,496 - MMJJ_RS07420 0.45 -2.1 1,362,497 + MMJJ_RS07420 0.45 -2.1 1,362,498 - MMJJ_RS07420 0.46 -0.8 1,362,498 - MMJJ_RS07420 0.46 -0.9 1,362,498 - MMJJ_RS07420 0.46 +3.8 1,362,511 + MMJJ_RS07420 0.48 -0.5 1,362,721 + -1.0 1,362,809 - +0.8 1,362,810 + -1.5 1,362,810 + -1.0 1,362,811 - -2.7 1,362,812 + -1.5 1,362,812 + -1.0 1,362,839 - -1.4 1,362,863 - MMJJ_RS07425 0.13 -1.8 1,363,093 + MMJJ_RS07425 0.41 -1.3 1,363,145 + MMJJ_RS07425 0.48 -1.7 1,363,145 + MMJJ_RS07425 0.48 -1.5 1,363,165 - MMJJ_RS07425 0.50 +0.3 1,363,165 - MMJJ_RS07425 0.50 -1.0 1,363,165 - MMJJ_RS07425 0.50 +0.7 1,363,166 + MMJJ_RS07425 0.50 -1.3 1,363,204 - MMJJ_RS07425 0.55 -0.9 1,363,204 - MMJJ_RS07425 0.55 +1.3 1,363,204 - MMJJ_RS07425 0.55 -0.8 1,363,212 + MMJJ_RS07425 0.56 -1.0 1,363,212 + MMJJ_RS07425 0.56 +0.3 1,363,212 + MMJJ_RS07425 0.56 -0.4 1,363,234 - MMJJ_RS07425 0.59 -2.3 1,363,234 - MMJJ_RS07425 0.59 -4.0 1,363,244 - MMJJ_RS07425 0.60 -0.5 1,363,260 - MMJJ_RS07425 0.62 -1.0 1,363,261 + MMJJ_RS07425 0.62 +0.6 1,363,261 + MMJJ_RS07425 0.62 +1.8 1,363,261 + MMJJ_RS07425 0.62 +0.3 1,363,274 + MMJJ_RS07425 0.64 -1.6
Or see this region's nucleotide sequence