Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07380 and MMJJ_RS07385 are separated by 163 nucleotides MMJJ_RS07385 and MMJJ_RS07390 are separated by 6 nucleotides MMJJ_RS07390 and MMJJ_RS07395 are separated by 16 nucleotides MMJJ_RS07395 and MMJJ_RS07400 are separated by 27 nucleotides
MMJJ_RS07380: MMJJ_RS07380 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,355,556 to 1,356,344
_RS07380
MMJJ_RS07385: MMJJ_RS07385 - ribosomal protein S18-alanine N-acetyltransferase, at 1,356,508 to 1,356,993
_RS07385
MMJJ_RS07390: MMJJ_RS07390 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,357,000 to 1,358,313
_RS07390
MMJJ_RS07395: MMJJ_RS07395 - NAD+ synthase, at 1,358,330 to 1,359,106
_RS07395
MMJJ_RS07400: MMJJ_RS07400 - 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,359,134 to 1,360,216
_RS07400
Position (kb)
1356
1357
1358
1359 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1356.035 kb on + strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.061 kb on + strand, within MMJJ_RS07380 at 1356.064 kb on - strand, within MMJJ_RS07380 at 1356.065 kb on + strand, within MMJJ_RS07380 at 1356.067 kb on - strand, within MMJJ_RS07380 at 1356.102 kb on + strand, within MMJJ_RS07380 at 1356.120 kb on + strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.205 kb on - strand, within MMJJ_RS07380 at 1356.206 kb on + strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.246 kb on - strand, within MMJJ_RS07380 at 1356.288 kb on - strand at 1356.338 kb on - strand at 1356.338 kb on - strand at 1356.339 kb on + strand at 1356.342 kb on - strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.367 kb on - strand at 1356.376 kb on + strand at 1356.404 kb on - strand at 1356.455 kb on + strand at 1356.455 kb on + strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.459 kb on + strand at 1356.460 kb on - strand at 1356.460 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.464 kb on + strand at 1356.468 kb on + strand at 1356.506 kb on - strand at 1356.506 kb on - strand at 1356.506 kb on - strand at 1356.507 kb on + strand at 1356.515 kb on - strand at 1356.519 kb on - strand at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.616 kb on - strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.641 kb on - strand, within MMJJ_RS07385 at 1356.677 kb on + strand, within MMJJ_RS07385 at 1356.717 kb on + strand, within MMJJ_RS07385 at 1356.735 kb on + strand, within MMJJ_RS07385 at 1356.735 kb on + strand, within MMJJ_RS07385 at 1356.748 kb on - strand, within MMJJ_RS07385 at 1356.749 kb on + strand, within MMJJ_RS07385 at 1356.771 kb on - strand, within MMJJ_RS07385 at 1356.771 kb on - strand, within MMJJ_RS07385 at 1356.772 kb on + strand, within MMJJ_RS07385 at 1356.866 kb on + strand, within MMJJ_RS07385 at 1356.867 kb on - strand, within MMJJ_RS07385 at 1356.867 kb on - strand, within MMJJ_RS07385 at 1356.868 kb on + strand, within MMJJ_RS07385 at 1356.868 kb on + strand, within MMJJ_RS07385 at 1356.878 kb on + strand, within MMJJ_RS07385 at 1356.885 kb on - strand, within MMJJ_RS07385 at 1356.900 kb on - strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.904 kb on - strand, within MMJJ_RS07385 at 1356.905 kb on + strand, within MMJJ_RS07385 at 1356.905 kb on + strand, within MMJJ_RS07385 at 1356.907 kb on - strand, within MMJJ_RS07385 at 1356.930 kb on + strand, within MMJJ_RS07385 at 1356.938 kb on - strand, within MMJJ_RS07385 at 1356.938 kb on - strand, within MMJJ_RS07385 at 1356.968 kb on + strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.992 kb on + strand at 1356.996 kb on + strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.031 kb on + strand at 1357.031 kb on + strand at 1357.031 kb on + strand at 1357.037 kb on + strand at 1357.037 kb on + strand at 1357.083 kb on - strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.086 kb on - strand at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.140 kb on + strand, within MMJJ_RS07390 at 1357.143 kb on - strand, within MMJJ_RS07390 at 1357.144 kb on + strand, within MMJJ_RS07390 at 1357.166 kb on - strand, within MMJJ_RS07390 at 1357.209 kb on + strand, within MMJJ_RS07390 at 1357.271 kb on - strand, within MMJJ_RS07390 at 1357.271 kb on - strand, within MMJJ_RS07390 at 1357.317 kb on - strand, within MMJJ_RS07390 at 1357.318 kb on + strand, within MMJJ_RS07390 at 1357.386 kb on - strand, within MMJJ_RS07390 at 1357.387 kb on + strand, within MMJJ_RS07390 at 1357.390 kb on + strand, within MMJJ_RS07390 at 1357.460 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.477 kb on - strand, within MMJJ_RS07390 at 1357.477 kb on - strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.479 kb on - strand, within MMJJ_RS07390 at 1357.574 kb on - strand, within MMJJ_RS07390 at 1357.648 kb on - strand, within MMJJ_RS07390 at 1357.710 kb on - strand, within MMJJ_RS07390 at 1357.711 kb on + strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.756 kb on - strand, within MMJJ_RS07390 at 1357.792 kb on - strand, within MMJJ_RS07390 at 1357.917 kb on + strand, within MMJJ_RS07390 at 1357.940 kb on - strand, within MMJJ_RS07390 at 1357.941 kb on + strand, within MMJJ_RS07390 at 1357.941 kb on + strand, within MMJJ_RS07390 at 1357.990 kb on + strand, within MMJJ_RS07390 at 1358.022 kb on + strand, within MMJJ_RS07390 at 1358.022 kb on + strand, within MMJJ_RS07390 at 1358.069 kb on - strand, within MMJJ_RS07390 at 1358.069 kb on - strand, within MMJJ_RS07390 at 1358.101 kb on + strand, within MMJJ_RS07390 at 1358.206 kb on + strand at 1358.206 kb on + strand at 1358.208 kb on - strand at 1358.209 kb on + strand at 1358.214 kb on - strand at 1358.214 kb on - strand at 1358.215 kb on + strand at 1358.228 kb on - strand at 1358.228 kb on - strand at 1358.228 kb on - strand at 1358.229 kb on + strand at 1358.229 kb on + strand at 1358.229 kb on + strand at 1358.230 kb on - strand at 1358.262 kb on - strand at 1358.264 kb on - strand at 1358.265 kb on + strand at 1358.265 kb on + strand at 1358.265 kb on + strand at 1358.282 kb on - strand at 1358.286 kb on + strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.290 kb on + strand at 1358.293 kb on - strand at 1358.311 kb on - strand at 1358.372 kb on + strand at 1358.425 kb on + strand, within MMJJ_RS07395 at 1358.861 kb on - strand, within MMJJ_RS07395 at 1359.114 kb on - strand at 1359.168 kb on - strand at 1359.237 kb on - strand at 1359.237 kb on - strand at 1359.238 kb on + strand at 1359.241 kb on + strand at 1359.261 kb on - strand, within MMJJ_RS07400 at 1359.269 kb on + strand, within MMJJ_RS07400 at 1359.306 kb on - strand, within MMJJ_RS07400
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,356,035 + MMJJ_RS07380 0.61 -0.2 1,356,049 - MMJJ_RS07380 0.62 -0.4 1,356,049 - MMJJ_RS07380 0.62 -1.7 1,356,061 + MMJJ_RS07380 0.64 -1.0 1,356,064 - MMJJ_RS07380 0.64 +0.3 1,356,065 + MMJJ_RS07380 0.65 -0.7 1,356,067 - MMJJ_RS07380 0.65 -0.9 1,356,102 + MMJJ_RS07380 0.69 +0.6 1,356,120 + MMJJ_RS07380 0.71 -1.0 1,356,188 - MMJJ_RS07380 0.80 +1.5 1,356,188 - MMJJ_RS07380 0.80 -1.2 1,356,205 - MMJJ_RS07380 0.82 -0.2 1,356,206 + MMJJ_RS07380 0.82 -2.7 1,356,219 - MMJJ_RS07380 0.84 -0.4 1,356,219 - MMJJ_RS07380 0.84 -0.2 1,356,220 + MMJJ_RS07380 0.84 +0.5 1,356,220 + MMJJ_RS07380 0.84 -0.7 1,356,246 - MMJJ_RS07380 0.87 -0.1 1,356,288 - -0.2 1,356,338 - -1.5 1,356,338 - +1.5 1,356,339 + -2.5 1,356,342 - +2.7 1,356,354 + -2.6 1,356,354 + -0.5 1,356,354 + -1.4 1,356,354 + -2.0 1,356,367 - +0.7 1,356,376 + -2.8 1,356,404 - +0.0 1,356,455 + +3.1 1,356,455 + -0.6 1,356,458 - +1.0 1,356,458 - -1.2 1,356,458 - +1.4 1,356,459 + -0.4 1,356,460 - +1.2 1,356,460 - +2.6 1,356,463 - -2.0 1,356,463 - -1.0 1,356,463 - -1.5 1,356,463 - -0.7 1,356,464 + +1.0 1,356,468 + +1.3 1,356,506 - -4.0 1,356,506 - -3.2 1,356,506 - -1.2 1,356,507 + +0.2 1,356,515 - -1.3 1,356,519 - +1.0 1,356,614 - MMJJ_RS07385 0.22 +0.4 1,356,614 - MMJJ_RS07385 0.22 +1.5 1,356,614 - MMJJ_RS07385 0.22 +1.3 1,356,614 - MMJJ_RS07385 0.22 -1.0 1,356,614 - MMJJ_RS07385 0.22 +0.4 1,356,614 - MMJJ_RS07385 0.22 -0.2 1,356,614 - MMJJ_RS07385 0.22 +0.3 1,356,615 + MMJJ_RS07385 0.22 +3.4 1,356,615 + MMJJ_RS07385 0.22 -0.2 1,356,615 + MMJJ_RS07385 0.22 +2.8 1,356,616 - MMJJ_RS07385 0.22 +1.9 1,356,617 + MMJJ_RS07385 0.22 +1.5 1,356,617 + MMJJ_RS07385 0.22 +0.7 1,356,617 + MMJJ_RS07385 0.22 -2.1 1,356,617 + MMJJ_RS07385 0.22 -2.0 1,356,641 - MMJJ_RS07385 0.27 -0.7 1,356,677 + MMJJ_RS07385 0.35 +0.6 1,356,717 + MMJJ_RS07385 0.43 +0.9 1,356,735 + MMJJ_RS07385 0.47 +0.7 1,356,735 + MMJJ_RS07385 0.47 -0.7 1,356,748 - MMJJ_RS07385 0.49 -1.0 1,356,749 + MMJJ_RS07385 0.50 -0.1 1,356,771 - MMJJ_RS07385 0.54 -0.2 1,356,771 - MMJJ_RS07385 0.54 +1.3 1,356,772 + MMJJ_RS07385 0.54 +2.7 1,356,866 + MMJJ_RS07385 0.74 -0.4 1,356,867 - MMJJ_RS07385 0.74 +1.9 1,356,867 - MMJJ_RS07385 0.74 -0.7 1,356,868 + MMJJ_RS07385 0.74 +3.3 1,356,868 + MMJJ_RS07385 0.74 -1.2 1,356,878 + MMJJ_RS07385 0.76 -1.5 1,356,885 - MMJJ_RS07385 0.78 -1.8 1,356,900 - MMJJ_RS07385 0.81 -1.1 1,356,901 + MMJJ_RS07385 0.81 -0.2 1,356,901 + MMJJ_RS07385 0.81 +0.5 1,356,901 + MMJJ_RS07385 0.81 -1.6 1,356,904 - MMJJ_RS07385 0.81 -3.0 1,356,905 + MMJJ_RS07385 0.82 +1.5 1,356,905 + MMJJ_RS07385 0.82 -0.3 1,356,907 - MMJJ_RS07385 0.82 -0.2 1,356,930 + MMJJ_RS07385 0.87 +0.3 1,356,938 - MMJJ_RS07385 0.88 -2.7 1,356,938 - MMJJ_RS07385 0.88 +0.1 1,356,968 + -1.7 1,356,991 - +1.4 1,356,991 - -2.7 1,356,991 - +2.3 1,356,991 - +1.1 1,356,992 + +2.7 1,356,996 + -3.0 1,357,028 - +1.9 1,357,028 - -0.7 1,357,028 - -0.9 1,357,028 - +5.3 1,357,028 - -0.9 1,357,028 - -1.0 1,357,028 - -3.6 1,357,028 - +0.5 1,357,028 - +0.1 1,357,028 - -0.9 1,357,028 - +2.8 1,357,029 + +1.3 1,357,029 + -1.1 1,357,029 + -0.2 1,357,029 + -0.7 1,357,029 + -0.9 1,357,029 + -1.8 1,357,030 - +1.0 1,357,030 - +4.1 1,357,030 - +1.2 1,357,030 - -2.8 1,357,030 - +0.3 1,357,030 - -0.9 1,357,030 - -1.0 1,357,031 + +2.7 1,357,031 + -1.6 1,357,031 + -1.7 1,357,037 + +0.0 1,357,037 + +0.9 1,357,083 - -0.3 1,357,084 + -2.4 1,357,084 + -1.5 1,357,084 + +2.7 1,357,084 + -1.0 1,357,084 + +0.7 1,357,086 - +1.3 1,357,139 - MMJJ_RS07390 0.11 +2.8 1,357,139 - MMJJ_RS07390 0.11 -0.5 1,357,139 - MMJJ_RS07390 0.11 +1.8 1,357,140 + MMJJ_RS07390 0.11 -0.6 1,357,143 - MMJJ_RS07390 0.11 -1.0 1,357,144 + MMJJ_RS07390 0.11 +2.3 1,357,166 - MMJJ_RS07390 0.13 -1.7 1,357,209 + MMJJ_RS07390 0.16 +5.3 1,357,271 - MMJJ_RS07390 0.21 +1.1 1,357,271 - MMJJ_RS07390 0.21 +2.0 1,357,317 - MMJJ_RS07390 0.24 +0.8 1,357,318 + MMJJ_RS07390 0.24 -2.8 1,357,386 - MMJJ_RS07390 0.29 -0.2 1,357,387 + MMJJ_RS07390 0.29 -2.4 1,357,390 + MMJJ_RS07390 0.30 +1.1 1,357,460 - MMJJ_RS07390 0.35 -2.0 1,357,475 - MMJJ_RS07390 0.36 -0.5 1,357,475 - MMJJ_RS07390 0.36 +3.2 1,357,475 - MMJJ_RS07390 0.36 -0.3 1,357,475 - MMJJ_RS07390 0.36 +1.3 1,357,476 + MMJJ_RS07390 0.36 -0.4 1,357,476 + MMJJ_RS07390 0.36 -0.4 1,357,476 + MMJJ_RS07390 0.36 -1.2 1,357,476 + MMJJ_RS07390 0.36 -0.7 1,357,476 + MMJJ_RS07390 0.36 -0.7 1,357,477 - MMJJ_RS07390 0.36 +1.6 1,357,477 - MMJJ_RS07390 0.36 -0.1 1,357,478 + MMJJ_RS07390 0.36 +1.0 1,357,478 + MMJJ_RS07390 0.36 +2.4 1,357,478 + MMJJ_RS07390 0.36 +4.0 1,357,478 + MMJJ_RS07390 0.36 +1.3 1,357,479 - MMJJ_RS07390 0.36 +2.9 1,357,574 - MMJJ_RS07390 0.44 +1.9 1,357,648 - MMJJ_RS07390 0.49 +0.3 1,357,710 - MMJJ_RS07390 0.54 +2.3 1,357,711 + MMJJ_RS07390 0.54 -1.0 1,357,712 - MMJJ_RS07390 0.54 +1.6 1,357,712 - MMJJ_RS07390 0.54 -2.2 1,357,712 - MMJJ_RS07390 0.54 +0.3 1,357,712 - MMJJ_RS07390 0.54 +2.2 1,357,713 + MMJJ_RS07390 0.54 +0.1 1,357,713 + MMJJ_RS07390 0.54 -3.6 1,357,713 + MMJJ_RS07390 0.54 +5.5 1,357,713 + MMJJ_RS07390 0.54 -2.0 1,357,713 + MMJJ_RS07390 0.54 +0.3 1,357,756 - MMJJ_RS07390 0.58 -0.9 1,357,792 - MMJJ_RS07390 0.60 -2.1 1,357,917 + MMJJ_RS07390 0.70 +1.1 1,357,940 - MMJJ_RS07390 0.72 -0.2 1,357,941 + MMJJ_RS07390 0.72 -2.0 1,357,941 + MMJJ_RS07390 0.72 +1.8 1,357,990 + MMJJ_RS07390 0.75 +0.6 1,358,022 + MMJJ_RS07390 0.78 +1.4 1,358,022 + MMJJ_RS07390 0.78 +1.3 1,358,069 - MMJJ_RS07390 0.81 -1.5 1,358,069 - MMJJ_RS07390 0.81 +0.3 1,358,101 + MMJJ_RS07390 0.84 +1.4 1,358,206 + +1.3 1,358,206 + +2.3 1,358,208 - +0.1 1,358,209 + +1.4 1,358,214 - +1.0 1,358,214 - +1.2 1,358,215 + -1.8 1,358,228 - +0.3 1,358,228 - -0.2 1,358,228 - -2.8 1,358,229 + +0.2 1,358,229 + +1.4 1,358,229 + +0.2 1,358,230 - -0.2 1,358,262 - +1.1 1,358,264 - -0.7 1,358,265 + +3.1 1,358,265 + -0.2 1,358,265 + -2.0 1,358,282 - -1.2 1,358,286 + -1.1 1,358,289 - -0.1 1,358,289 - +3.2 1,358,289 - +1.2 1,358,289 - +0.8 1,358,289 - +2.7 1,358,290 + -0.1 1,358,293 - +1.4 1,358,311 - +1.3 1,358,372 + -0.7 1,358,425 + MMJJ_RS07395 0.12 +0.3 1,358,861 - MMJJ_RS07395 0.68 +2.1 1,359,114 - -0.9 1,359,168 - +2.1 1,359,237 - -0.0 1,359,237 - -2.0 1,359,238 + -1.7 1,359,241 + +1.6 1,359,261 - MMJJ_RS07400 0.12 -0.4 1,359,269 + MMJJ_RS07400 0.12 -1.0 1,359,306 - MMJJ_RS07400 0.16 -0.2
Or see this region's nucleotide sequence