Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07000 and MMJJ_RS07005 are separated by 71 nucleotides MMJJ_RS07005 and MMJJ_RS07010 are separated by 54 nucleotides MMJJ_RS07010 and MMJJ_RS07015 are separated by 72 nucleotides
MMJJ_RS07000: MMJJ_RS07000 - TrkH family potassium uptake protein, at 1,285,117 to 1,286,553
_RS07000
MMJJ_RS07005: MMJJ_RS07005 - transcription factor S, at 1,286,625 to 1,286,942
_RS07005
MMJJ_RS07010: MMJJ_RS07010 - preprotein translocase subunit Sec61beta, at 1,286,997 to 1,287,158
_RS07010
MMJJ_RS07015: MMJJ_RS07015 - PFL family protein, at 1,287,231 to 1,288,607
_RS07015
Position (kb)
1286
1287
1288 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1286.058 kb on + strand at 1286.265 kb on + strand, within MMJJ_RS07000 at 1286.278 kb on + strand at 1286.278 kb on + strand, within MMJJ_RS07000 at 1286.480 kb on - strand at 1286.498 kb on + strand at 1286.521 kb on + strand at 1286.550 kb on + strand at 1286.551 kb on - strand at 1286.551 kb on - strand at 1286.552 kb on + strand at 1286.556 kb on + strand at 1286.582 kb on - strand at 1286.590 kb on - strand at 1286.590 kb on - strand at 1286.590 kb on - strand at 1286.591 kb on + strand at 1286.591 kb on + strand at 1286.593 kb on + strand at 1286.600 kb on + strand at 1286.602 kb on + strand at 1286.602 kb on + strand at 1286.624 kb on - strand at 1286.624 kb on - strand at 1286.624 kb on - strand at 1286.625 kb on + strand at 1286.642 kb on - strand at 1286.654 kb on - strand at 1286.734 kb on + strand, within MMJJ_RS07005 at 1286.741 kb on + strand, within MMJJ_RS07005 at 1286.741 kb on + strand, within MMJJ_RS07005 at 1286.774 kb on - strand, within MMJJ_RS07005 at 1286.792 kb on + strand, within MMJJ_RS07005 at 1286.815 kb on - strand, within MMJJ_RS07005 at 1286.815 kb on - strand, within MMJJ_RS07005 at 1286.857 kb on - strand, within MMJJ_RS07005 at 1286.899 kb on + strand, within MMJJ_RS07005 at 1286.921 kb on - strand at 1286.934 kb on - strand at 1286.945 kb on + strand at 1286.962 kb on - strand at 1287.037 kb on + strand, within MMJJ_RS07010 at 1287.038 kb on - strand, within MMJJ_RS07010 at 1287.039 kb on + strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.106 kb on - strand, within MMJJ_RS07010 at 1287.106 kb on - strand, within MMJJ_RS07010 at 1287.106 kb on - strand at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.111 kb on - strand, within MMJJ_RS07010 at 1287.112 kb on + strand, within MMJJ_RS07010 at 1287.112 kb on + strand, within MMJJ_RS07010 at 1287.153 kb on + strand at 1287.169 kb on - strand at 1287.169 kb on - strand at 1287.180 kb on - strand at 1287.181 kb on + strand at 1287.181 kb on + strand at 1287.181 kb on + strand at 1287.181 kb on + strand at 1287.182 kb on - strand at 1287.182 kb on - strand at 1287.182 kb on - strand at 1287.183 kb on + strand at 1287.183 kb on + strand at 1287.224 kb on + strand at 1288.106 kb on + strand, within MMJJ_RS07015 at 1288.152 kb on - strand, within MMJJ_RS07015
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,286,058 + +1.4 1,286,265 + MMJJ_RS07000 0.80 -1.2 1,286,278 + +0.4 1,286,278 + MMJJ_RS07000 0.81 -0.2 1,286,480 - +2.1 1,286,498 + -0.6 1,286,521 + -1.8 1,286,550 + -1.0 1,286,551 - -3.1 1,286,551 - +0.8 1,286,552 + +2.6 1,286,556 + -2.6 1,286,582 - -1.2 1,286,590 - +2.5 1,286,590 - -1.2 1,286,590 - -0.2 1,286,591 + -2.6 1,286,591 + +1.7 1,286,593 + -2.1 1,286,600 + +0.7 1,286,602 + -2.1 1,286,602 + -0.9 1,286,624 - -3.0 1,286,624 - -0.2 1,286,624 - -1.7 1,286,625 + -2.5 1,286,642 - +0.9 1,286,654 - +1.4 1,286,734 + MMJJ_RS07005 0.34 -0.9 1,286,741 + MMJJ_RS07005 0.36 -2.6 1,286,741 + MMJJ_RS07005 0.36 -1.7 1,286,774 - MMJJ_RS07005 0.47 -1.1 1,286,792 + MMJJ_RS07005 0.53 +2.6 1,286,815 - MMJJ_RS07005 0.60 -1.4 1,286,815 - MMJJ_RS07005 0.60 -0.7 1,286,857 - MMJJ_RS07005 0.73 -2.9 1,286,899 + MMJJ_RS07005 0.86 -2.5 1,286,921 - -0.5 1,286,934 - +0.2 1,286,945 + -1.5 1,286,962 - +2.9 1,287,037 + MMJJ_RS07010 0.25 +4.8 1,287,038 - MMJJ_RS07010 0.25 -2.1 1,287,039 + MMJJ_RS07010 0.26 +1.3 1,287,104 - MMJJ_RS07010 0.66 +3.3 1,287,104 - MMJJ_RS07010 0.66 +1.4 1,287,104 - MMJJ_RS07010 0.66 +2.5 1,287,104 - MMJJ_RS07010 0.66 +2.1 1,287,105 + MMJJ_RS07010 0.67 -1.2 1,287,105 + MMJJ_RS07010 0.67 +0.1 1,287,105 + MMJJ_RS07010 0.67 -1.4 1,287,105 + MMJJ_RS07010 0.67 +1.7 1,287,106 - MMJJ_RS07010 0.67 -0.6 1,287,106 - MMJJ_RS07010 0.67 -2.1 1,287,106 - +1.4 1,287,107 + MMJJ_RS07010 0.68 +1.2 1,287,107 + MMJJ_RS07010 0.68 -3.0 1,287,107 + MMJJ_RS07010 0.68 +0.1 1,287,107 + MMJJ_RS07010 0.68 +0.2 1,287,111 - MMJJ_RS07010 0.70 +0.9 1,287,112 + MMJJ_RS07010 0.71 -1.5 1,287,112 + MMJJ_RS07010 0.71 -1.2 1,287,153 + -0.0 1,287,169 - +0.8 1,287,169 - -3.0 1,287,180 - +1.6 1,287,181 + +0.7 1,287,181 + +2.3 1,287,181 + -0.3 1,287,181 + +1.1 1,287,182 - +1.4 1,287,182 - -3.0 1,287,182 - +0.2 1,287,183 + -0.2 1,287,183 + -0.5 1,287,224 + -0.2 1,288,106 + MMJJ_RS07015 0.64 +1.4 1,288,152 - MMJJ_RS07015 0.67 +0.7
Or see this region's nucleotide sequence