Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06825 and MMJJ_RS06830 are separated by 20 nucleotides MMJJ_RS06830 and MMJJ_RS06835 overlap by 8 nucleotides MMJJ_RS06835 and MMJJ_RS06840 are separated by 28 nucleotides
MMJJ_RS06825: MMJJ_RS06825 - 4Fe-4S binding protein, at 1,260,121 to 1,260,879
_RS06825
MMJJ_RS06830: MMJJ_RS06830 - 4Fe-4S binding protein, at 1,260,900 to 1,261,385
_RS06830
MMJJ_RS06835: MMJJ_RS06835 - 4Fe-4S binding protein, at 1,261,378 to 1,262,559
_RS06835
MMJJ_RS06840: MMJJ_RS06840 - nickel-dependent hydrogenase large subunit, at 1,262,588 to 1,263,715
_RS06840
Position (kb)
1261
1262
1263 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1260.378 kb on - strand, within MMJJ_RS06825 at 1260.379 kb on + strand, within MMJJ_RS06825 at 1260.398 kb on + strand, within MMJJ_RS06825 at 1260.403 kb on - strand, within MMJJ_RS06825 at 1260.403 kb on - strand, within MMJJ_RS06825 at 1260.404 kb on + strand, within MMJJ_RS06825 at 1260.404 kb on + strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.505 kb on - strand, within MMJJ_RS06825 at 1260.505 kb on - strand, within MMJJ_RS06825 at 1260.515 kb on + strand, within MMJJ_RS06825 at 1260.529 kb on - strand, within MMJJ_RS06825 at 1260.530 kb on + strand, within MMJJ_RS06825 at 1260.556 kb on - strand, within MMJJ_RS06825 at 1260.557 kb on + strand, within MMJJ_RS06825 at 1260.590 kb on + strand, within MMJJ_RS06825 at 1260.732 kb on - strand, within MMJJ_RS06825 at 1260.733 kb on + strand, within MMJJ_RS06825 at 1260.733 kb on + strand, within MMJJ_RS06825 at 1260.751 kb on - strand, within MMJJ_RS06825 at 1260.751 kb on - strand, within MMJJ_RS06825 at 1260.837 kb on - strand at 1260.874 kb on - strand at 1260.898 kb on - strand at 1260.898 kb on - strand at 1260.902 kb on + strand at 1260.931 kb on + strand at 1260.980 kb on - strand, within MMJJ_RS06830 at 1260.981 kb on + strand, within MMJJ_RS06830 at 1261.025 kb on - strand, within MMJJ_RS06830 at 1261.029 kb on - strand, within MMJJ_RS06830 at 1261.061 kb on + strand, within MMJJ_RS06830 at 1261.061 kb on + strand, within MMJJ_RS06830 at 1261.124 kb on - strand, within MMJJ_RS06830 at 1261.130 kb on - strand, within MMJJ_RS06830 at 1261.191 kb on + strand, within MMJJ_RS06830 at 1261.191 kb on + strand, within MMJJ_RS06830 at 1261.259 kb on - strand, within MMJJ_RS06830 at 1261.260 kb on + strand, within MMJJ_RS06830 at 1261.260 kb on + strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.352 kb on + strand at 1261.440 kb on - strand at 1261.454 kb on + strand at 1261.454 kb on + strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.460 kb on + strand at 1261.460 kb on + strand at 1261.460 kb on + strand at 1261.520 kb on + strand, within MMJJ_RS06835 at 1261.535 kb on - strand, within MMJJ_RS06835 at 1261.561 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.612 kb on - strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.620 kb on + strand, within MMJJ_RS06835 at 1261.635 kb on + strand, within MMJJ_RS06835 at 1261.661 kb on + strand, within MMJJ_RS06835 at 1261.680 kb on - strand, within MMJJ_RS06835 at 1261.681 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.734 kb on + strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.823 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.900 kb on - strand, within MMJJ_RS06835 at 1261.901 kb on + strand, within MMJJ_RS06835 at 1261.901 kb on + strand, within MMJJ_RS06835 at 1261.934 kb on + strand, within MMJJ_RS06835 at 1261.984 kb on + strand, within MMJJ_RS06835 at 1261.986 kb on - strand, within MMJJ_RS06835 at 1262.030 kb on - strand, within MMJJ_RS06835 at 1262.031 kb on + strand, within MMJJ_RS06835 at 1262.035 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.083 kb on - strand, within MMJJ_RS06835 at 1262.101 kb on - strand, within MMJJ_RS06835 at 1262.101 kb on - strand, within MMJJ_RS06835 at 1262.102 kb on + strand, within MMJJ_RS06835 at 1262.108 kb on - strand, within MMJJ_RS06835 at 1262.109 kb on + strand, within MMJJ_RS06835 at 1262.174 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.197 kb on - strand, within MMJJ_RS06835 at 1262.197 kb on - strand, within MMJJ_RS06835 at 1262.198 kb on + strand, within MMJJ_RS06835 at 1262.198 kb on + strand, within MMJJ_RS06835 at 1262.306 kb on + strand, within MMJJ_RS06835 at 1262.394 kb on - strand, within MMJJ_RS06835 at 1262.410 kb on - strand, within MMJJ_RS06835 at 1262.411 kb on + strand, within MMJJ_RS06835 at 1262.411 kb on + strand, within MMJJ_RS06835 at 1262.413 kb on + strand, within MMJJ_RS06835 at 1262.469 kb on - strand at 1262.500 kb on + strand at 1262.509 kb on + strand at 1262.541 kb on + strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.582 kb on - strand at 1262.586 kb on - strand at 1262.617 kb on - strand at 1262.617 kb on - strand at 1262.621 kb on - strand at 1262.621 kb on - strand at 1262.630 kb on - strand at 1262.630 kb on - strand at 1262.638 kb on - strand at 1262.639 kb on + strand at 1262.650 kb on - strand at 1262.650 kb on - strand at 1262.680 kb on + strand at 1262.680 kb on + strand at 1262.680 kb on + strand at 1262.724 kb on - strand, within MMJJ_RS06840 at 1262.725 kb on + strand, within MMJJ_RS06840 at 1262.777 kb on - strand, within MMJJ_RS06840 at 1263.127 kb on - strand, within MMJJ_RS06840 at 1263.271 kb on - strand, within MMJJ_RS06840 at 1263.285 kb on + strand, within MMJJ_RS06840 at 1263.299 kb on + strand, within MMJJ_RS06840 at 1263.430 kb on - strand, within MMJJ_RS06840 at 1263.435 kb on + strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,260,378 - MMJJ_RS06825 0.34 +1.9 1,260,379 + MMJJ_RS06825 0.34 -1.4 1,260,398 + MMJJ_RS06825 0.36 +0.6 1,260,403 - MMJJ_RS06825 0.37 -3.0 1,260,403 - MMJJ_RS06825 0.37 -0.9 1,260,404 + MMJJ_RS06825 0.37 +0.2 1,260,404 + MMJJ_RS06825 0.37 -1.9 1,260,475 - MMJJ_RS06825 0.47 +0.0 1,260,475 - MMJJ_RS06825 0.47 -1.9 1,260,475 - MMJJ_RS06825 0.47 -2.7 1,260,505 - MMJJ_RS06825 0.51 -0.6 1,260,505 - MMJJ_RS06825 0.51 +0.5 1,260,515 + MMJJ_RS06825 0.52 -1.0 1,260,529 - MMJJ_RS06825 0.54 -1.6 1,260,530 + MMJJ_RS06825 0.54 -2.4 1,260,556 - MMJJ_RS06825 0.57 -2.2 1,260,557 + MMJJ_RS06825 0.57 -0.7 1,260,590 + MMJJ_RS06825 0.62 -1.5 1,260,732 - MMJJ_RS06825 0.81 -1.7 1,260,733 + MMJJ_RS06825 0.81 -0.6 1,260,733 + MMJJ_RS06825 0.81 -1.4 1,260,751 - MMJJ_RS06825 0.83 -0.3 1,260,751 - MMJJ_RS06825 0.83 -0.5 1,260,837 - -1.3 1,260,874 - -0.0 1,260,898 - -1.2 1,260,898 - -2.2 1,260,902 + -0.9 1,260,931 + -2.3 1,260,980 - MMJJ_RS06830 0.16 -1.7 1,260,981 + MMJJ_RS06830 0.17 -0.7 1,261,025 - MMJJ_RS06830 0.26 -1.0 1,261,029 - MMJJ_RS06830 0.27 -2.8 1,261,061 + MMJJ_RS06830 0.33 -2.7 1,261,061 + MMJJ_RS06830 0.33 +1.4 1,261,124 - MMJJ_RS06830 0.46 -1.6 1,261,130 - MMJJ_RS06830 0.47 +0.1 1,261,191 + MMJJ_RS06830 0.60 -1.5 1,261,191 + MMJJ_RS06830 0.60 +1.0 1,261,259 - MMJJ_RS06830 0.74 -0.5 1,261,260 + MMJJ_RS06830 0.74 -1.5 1,261,260 + MMJJ_RS06830 0.74 -0.6 1,261,309 - MMJJ_RS06830 0.84 +0.5 1,261,309 - MMJJ_RS06830 0.84 -0.9 1,261,309 - MMJJ_RS06830 0.84 -0.5 1,261,310 + MMJJ_RS06830 0.84 -2.7 1,261,310 + MMJJ_RS06830 0.84 -1.3 1,261,310 + MMJJ_RS06830 0.84 +1.8 1,261,352 + -1.3 1,261,440 - -2.2 1,261,454 + +3.0 1,261,454 + -1.7 1,261,459 - -0.2 1,261,459 - -1.2 1,261,459 - -0.5 1,261,459 - -1.1 1,261,459 - -0.9 1,261,460 + -2.4 1,261,460 + -1.6 1,261,460 + -2.4 1,261,520 + MMJJ_RS06835 0.12 -0.4 1,261,535 - MMJJ_RS06835 0.13 +0.2 1,261,561 + MMJJ_RS06835 0.15 -1.6 1,261,601 + MMJJ_RS06835 0.19 -0.2 1,261,601 + MMJJ_RS06835 0.19 -1.6 1,261,612 - MMJJ_RS06835 0.20 -2.6 1,261,613 + MMJJ_RS06835 0.20 -0.2 1,261,613 + MMJJ_RS06835 0.20 -2.2 1,261,620 + MMJJ_RS06835 0.20 -1.6 1,261,635 + MMJJ_RS06835 0.22 -0.6 1,261,661 + MMJJ_RS06835 0.24 -1.8 1,261,680 - MMJJ_RS06835 0.26 -0.6 1,261,681 + MMJJ_RS06835 0.26 -1.6 1,261,692 + MMJJ_RS06835 0.27 -0.7 1,261,692 + MMJJ_RS06835 0.27 +0.4 1,261,734 + MMJJ_RS06835 0.30 -2.7 1,261,822 - MMJJ_RS06835 0.38 -0.1 1,261,822 - MMJJ_RS06835 0.38 -1.1 1,261,823 + MMJJ_RS06835 0.38 -1.0 1,261,840 + MMJJ_RS06835 0.39 -0.4 1,261,840 + MMJJ_RS06835 0.39 -3.1 1,261,855 - MMJJ_RS06835 0.40 -2.8 1,261,855 - MMJJ_RS06835 0.40 -0.8 1,261,897 + MMJJ_RS06835 0.44 +0.4 1,261,897 + MMJJ_RS06835 0.44 -1.8 1,261,897 + MMJJ_RS06835 0.44 -1.8 1,261,900 - MMJJ_RS06835 0.44 +0.8 1,261,901 + MMJJ_RS06835 0.44 -2.7 1,261,901 + MMJJ_RS06835 0.44 -1.5 1,261,934 + MMJJ_RS06835 0.47 -0.9 1,261,984 + MMJJ_RS06835 0.51 -2.6 1,261,986 - MMJJ_RS06835 0.51 +1.1 1,262,030 - MMJJ_RS06835 0.55 +0.8 1,262,031 + MMJJ_RS06835 0.55 +0.6 1,262,035 + MMJJ_RS06835 0.56 -1.8 1,262,060 + MMJJ_RS06835 0.58 -1.4 1,262,060 + MMJJ_RS06835 0.58 -3.0 1,262,060 + MMJJ_RS06835 0.58 -3.0 1,262,083 - MMJJ_RS06835 0.60 -1.6 1,262,101 - MMJJ_RS06835 0.61 -2.6 1,262,101 - MMJJ_RS06835 0.61 -2.3 1,262,102 + MMJJ_RS06835 0.61 -1.9 1,262,108 - MMJJ_RS06835 0.62 -0.8 1,262,109 + MMJJ_RS06835 0.62 -0.9 1,262,174 + MMJJ_RS06835 0.67 -2.9 1,262,195 + MMJJ_RS06835 0.69 -1.4 1,262,195 + MMJJ_RS06835 0.69 -0.0 1,262,195 + MMJJ_RS06835 0.69 +1.4 1,262,197 - MMJJ_RS06835 0.69 +0.8 1,262,197 - MMJJ_RS06835 0.69 -2.2 1,262,198 + MMJJ_RS06835 0.69 -3.0 1,262,198 + MMJJ_RS06835 0.69 -1.2 1,262,306 + MMJJ_RS06835 0.79 +0.1 1,262,394 - MMJJ_RS06835 0.86 -1.8 1,262,410 - MMJJ_RS06835 0.87 -1.9 1,262,411 + MMJJ_RS06835 0.87 -1.2 1,262,411 + MMJJ_RS06835 0.87 -0.4 1,262,413 + MMJJ_RS06835 0.88 -3.1 1,262,469 - +0.4 1,262,500 + -2.2 1,262,509 + -2.1 1,262,541 + -3.0 1,262,559 - +0.5 1,262,559 - -0.5 1,262,559 - -2.6 1,262,559 - -2.1 1,262,559 - -0.8 1,262,559 - -1.9 1,262,559 - -1.4 1,262,559 - +0.4 1,262,560 + -0.9 1,262,560 + -1.5 1,262,560 + -1.1 1,262,560 + -2.1 1,262,560 + -0.9 1,262,560 + -2.2 1,262,560 + -0.2 1,262,563 - -3.0 1,262,563 - +1.4 1,262,564 + -1.2 1,262,564 + +0.4 1,262,564 + -1.3 1,262,564 + -0.9 1,262,564 + -3.7 1,262,582 - -1.7 1,262,586 - -0.7 1,262,617 - -1.5 1,262,617 - -2.1 1,262,621 - +0.0 1,262,621 - -0.9 1,262,630 - -2.3 1,262,630 - -1.1 1,262,638 - -0.2 1,262,639 + -1.2 1,262,650 - -0.6 1,262,650 - -0.9 1,262,680 + +1.4 1,262,680 + -1.2 1,262,680 + +0.1 1,262,724 - MMJJ_RS06840 0.12 -1.2 1,262,725 + MMJJ_RS06840 0.12 -0.2 1,262,777 - MMJJ_RS06840 0.17 +0.4 1,263,127 - MMJJ_RS06840 0.48 -0.2 1,263,271 - MMJJ_RS06840 0.61 +1.4 1,263,285 + MMJJ_RS06840 0.62 +0.4 1,263,299 + MMJJ_RS06840 0.63 +1.4 1,263,430 - MMJJ_RS06840 0.75 +0.4 1,263,435 + MMJJ_RS06840 0.75 -0.6 1,263,516 - MMJJ_RS06840 0.82 -2.6 1,263,516 - MMJJ_RS06840 0.82 +1.4 1,263,553 - +1.4
Or see this region's nucleotide sequence