Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06790 and MMJJ_RS06795 are separated by 101 nucleotides MMJJ_RS06795 and MMJJ_RS06800 are separated by 126 nucleotides MMJJ_RS06800 and MMJJ_RS06805 are separated by 194 nucleotides MMJJ_RS06805 and MMJJ_RS06810 are separated by 54 nucleotides
MMJJ_RS06790: MMJJ_RS06790 - nucleotidyltransferase family protein, at 1,255,502 to 1,256,551
_RS06790
MMJJ_RS06795: MMJJ_RS06795 - tRNA-Val, at 1,256,653 to 1,256,729
_RS06795
MMJJ_RS06800: MMJJ_RS06800 - prefoldin subunit alpha, at 1,256,856 to 1,257,290
_RS06800
MMJJ_RS06805: MMJJ_RS06805 - monovalent cation/H+ antiporter subunit E, at 1,257,485 to 1,257,787
_RS06805
MMJJ_RS06810: MMJJ_RS06810 - L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha, at 1,257,842 to 1,259,035
_RS06810
Position (kb)
1257
1258 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1256.508 kb on + strand at 1256.740 kb on + strand at 1256.799 kb on - strand at 1256.799 kb on - strand at 1256.800 kb on + strand at 1256.800 kb on + strand at 1256.804 kb on - strand at 1256.810 kb on + strand at 1256.812 kb on - strand at 1256.812 kb on - strand at 1256.813 kb on + strand at 1256.813 kb on + strand at 1256.813 kb on + strand at 1256.816 kb on - strand at 1256.816 kb on - strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.858 kb on + strand at 1256.867 kb on - strand at 1257.003 kb on + strand, within MMJJ_RS06800 at 1257.018 kb on - strand, within MMJJ_RS06800 at 1257.019 kb on + strand, within MMJJ_RS06800 at 1257.024 kb on - strand, within MMJJ_RS06800 at 1257.024 kb on - strand, within MMJJ_RS06800 at 1257.025 kb on + strand, within MMJJ_RS06800 at 1257.025 kb on + strand, within MMJJ_RS06800 at 1257.025 kb on + strand, within MMJJ_RS06800 at 1257.033 kb on - strand, within MMJJ_RS06800 at 1257.033 kb on - strand, within MMJJ_RS06800 at 1257.034 kb on + strand, within MMJJ_RS06800 at 1257.034 kb on + strand, within MMJJ_RS06800 at 1257.049 kb on + strand, within MMJJ_RS06800 at 1257.049 kb on + strand, within MMJJ_RS06800 at 1257.200 kb on - strand, within MMJJ_RS06800 at 1257.201 kb on + strand, within MMJJ_RS06800 at 1257.201 kb on + strand, within MMJJ_RS06800 at 1257.201 kb on + strand, within MMJJ_RS06800 at 1257.303 kb on - strand at 1257.304 kb on + strand at 1257.304 kb on + strand at 1257.306 kb on - strand at 1257.306 kb on - strand at 1257.307 kb on + strand at 1257.307 kb on + strand at 1257.327 kb on + strand at 1257.336 kb on - strand at 1257.337 kb on + strand at 1257.337 kb on + strand at 1257.349 kb on - strand at 1257.375 kb on - strand at 1257.376 kb on + strand at 1257.376 kb on + strand at 1257.383 kb on - strand at 1257.384 kb on + strand at 1257.390 kb on - strand at 1257.420 kb on - strand at 1257.420 kb on - strand at 1257.420 kb on - strand at 1257.421 kb on + strand at 1257.421 kb on + strand at 1257.421 kb on + strand at 1257.421 kb on + strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.427 kb on + strand at 1257.427 kb on + strand at 1257.430 kb on - strand at 1257.483 kb on + strand at 1257.555 kb on - strand, within MMJJ_RS06805 at 1257.556 kb on + strand, within MMJJ_RS06805 at 1257.556 kb on + strand, within MMJJ_RS06805 at 1257.615 kb on - strand, within MMJJ_RS06805 at 1257.724 kb on + strand, within MMJJ_RS06805 at 1257.747 kb on - strand, within MMJJ_RS06805 at 1257.764 kb on - strand at 1257.764 kb on - strand at 1257.768 kb on - strand at 1257.769 kb on + strand at 1257.785 kb on - strand at 1257.808 kb on + strand at 1257.841 kb on - strand at 1257.859 kb on - strand at 1257.859 kb on - strand at 1257.860 kb on + strand at 1257.860 kb on + strand at 1257.945 kb on - strand at 1257.945 kb on - strand at 1257.946 kb on + strand at 1257.950 kb on + strand at 1257.952 kb on - strand at 1257.953 kb on + strand at 1257.953 kb on + strand at 1257.988 kb on - strand, within MMJJ_RS06810 at 1257.989 kb on + strand, within MMJJ_RS06810 at 1257.993 kb on - strand, within MMJJ_RS06810 at 1258.017 kb on - strand, within MMJJ_RS06810 at 1258.018 kb on + strand, within MMJJ_RS06810 at 1258.020 kb on + strand, within MMJJ_RS06810 at 1258.039 kb on - strand, within MMJJ_RS06810 at 1258.056 kb on - strand, within MMJJ_RS06810 at 1258.056 kb on - strand, within MMJJ_RS06810 at 1258.093 kb on - strand, within MMJJ_RS06810 at 1258.127 kb on - strand, within MMJJ_RS06810 at 1258.176 kb on - strand, within MMJJ_RS06810 at 1258.177 kb on + strand, within MMJJ_RS06810 at 1258.180 kb on - strand, within MMJJ_RS06810 at 1258.180 kb on - strand, within MMJJ_RS06810 at 1258.203 kb on - strand, within MMJJ_RS06810 at 1258.211 kb on + strand, within MMJJ_RS06810 at 1258.217 kb on + strand, within MMJJ_RS06810 at 1258.260 kb on - strand, within MMJJ_RS06810 at 1258.270 kb on + strand, within MMJJ_RS06810 at 1258.295 kb on + strand, within MMJJ_RS06810 at 1258.309 kb on + strand, within MMJJ_RS06810 at 1258.359 kb on - strand, within MMJJ_RS06810 at 1258.360 kb on + strand, within MMJJ_RS06810 at 1258.364 kb on - strand, within MMJJ_RS06810 at 1258.364 kb on - strand, within MMJJ_RS06810 at 1258.364 kb on - strand, within MMJJ_RS06810 at 1258.365 kb on + strand, within MMJJ_RS06810 at 1258.365 kb on + strand, within MMJJ_RS06810 at 1258.365 kb on + strand, within MMJJ_RS06810 at 1258.416 kb on + strand, within MMJJ_RS06810 at 1258.416 kb on + strand, within MMJJ_RS06810 at 1258.417 kb on - strand, within MMJJ_RS06810 at 1258.418 kb on + strand, within MMJJ_RS06810 at 1258.424 kb on + strand, within MMJJ_RS06810 at 1258.432 kb on + strand, within MMJJ_RS06810 at 1258.454 kb on - strand, within MMJJ_RS06810 at 1258.486 kb on - strand, within MMJJ_RS06810 at 1258.486 kb on - strand, within MMJJ_RS06810 at 1258.486 kb on - strand, within MMJJ_RS06810 at 1258.487 kb on + strand, within MMJJ_RS06810 at 1258.528 kb on - strand, within MMJJ_RS06810 at 1258.529 kb on + strand, within MMJJ_RS06810 at 1258.529 kb on + strand, within MMJJ_RS06810 at 1258.559 kb on + strand, within MMJJ_RS06810 at 1258.559 kb on + strand, within MMJJ_RS06810 at 1258.559 kb on + strand, within MMJJ_RS06810 at 1258.587 kb on - strand, within MMJJ_RS06810 at 1258.596 kb on + strand, within MMJJ_RS06810 at 1258.630 kb on + strand, within MMJJ_RS06810 at 1258.667 kb on - strand, within MMJJ_RS06810 at 1258.678 kb on - strand, within MMJJ_RS06810 at 1258.678 kb on - strand, within MMJJ_RS06810 at 1258.679 kb on + strand, within MMJJ_RS06810 at 1258.679 kb on + strand, within MMJJ_RS06810 at 1258.679 kb on + strand, within MMJJ_RS06810 at 1258.679 kb on + strand, within MMJJ_RS06810 at 1258.688 kb on - strand, within MMJJ_RS06810 at 1258.718 kb on + strand, within MMJJ_RS06810 at 1258.718 kb on + strand, within MMJJ_RS06810 at 1258.718 kb on + strand, within MMJJ_RS06810 at 1258.737 kb on - strand, within MMJJ_RS06810
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,256,508 + -2.8 1,256,740 + +0.4 1,256,799 - -1.2 1,256,799 - +2.4 1,256,800 + -2.1 1,256,800 + -1.9 1,256,804 - -2.1 1,256,810 + -0.9 1,256,812 - -2.8 1,256,812 - -1.3 1,256,813 + +3.0 1,256,813 + -0.9 1,256,813 + -2.8 1,256,816 - +1.2 1,256,816 - -1.6 1,256,817 + -1.6 1,256,817 + -0.9 1,256,817 + -0.6 1,256,817 + +1.3 1,256,817 + -0.6 1,256,858 + -0.2 1,256,867 - -1.8 1,257,003 + MMJJ_RS06800 0.34 +3.9 1,257,018 - MMJJ_RS06800 0.37 +1.0 1,257,019 + MMJJ_RS06800 0.37 -0.6 1,257,024 - MMJJ_RS06800 0.39 -1.4 1,257,024 - MMJJ_RS06800 0.39 -0.6 1,257,025 + MMJJ_RS06800 0.39 -1.9 1,257,025 + MMJJ_RS06800 0.39 -1.1 1,257,025 + MMJJ_RS06800 0.39 +0.2 1,257,033 - MMJJ_RS06800 0.41 +0.4 1,257,033 - MMJJ_RS06800 0.41 +1.1 1,257,034 + MMJJ_RS06800 0.41 -2.3 1,257,034 + MMJJ_RS06800 0.41 +4.4 1,257,049 + MMJJ_RS06800 0.44 +1.4 1,257,049 + MMJJ_RS06800 0.44 -0.4 1,257,200 - MMJJ_RS06800 0.79 -1.4 1,257,201 + MMJJ_RS06800 0.79 +1.9 1,257,201 + MMJJ_RS06800 0.79 -1.2 1,257,201 + MMJJ_RS06800 0.79 +0.1 1,257,303 - +2.4 1,257,304 + -1.5 1,257,304 + -1.2 1,257,306 - -1.2 1,257,306 - -2.2 1,257,307 + -0.8 1,257,307 + -1.2 1,257,327 + -1.0 1,257,336 - -1.2 1,257,337 + -0.7 1,257,337 + +0.9 1,257,349 - -2.6 1,257,375 - -0.6 1,257,376 + +4.2 1,257,376 + +0.9 1,257,383 - +0.8 1,257,384 + -1.3 1,257,390 - +3.6 1,257,420 - -1.2 1,257,420 - -0.2 1,257,420 - +0.8 1,257,421 + +2.9 1,257,421 + +2.3 1,257,421 + +2.1 1,257,421 + -0.9 1,257,422 - -0.6 1,257,422 - +0.1 1,257,422 - -0.6 1,257,422 - -1.2 1,257,422 - +1.0 1,257,422 - +0.9 1,257,422 - -2.7 1,257,422 - -0.7 1,257,422 - +2.4 1,257,422 - +0.2 1,257,422 - +0.4 1,257,423 + +1.7 1,257,423 + -0.3 1,257,423 + -0.6 1,257,423 + +0.4 1,257,423 + -0.6 1,257,423 + +1.6 1,257,423 + +1.6 1,257,425 + -0.9 1,257,425 + -0.9 1,257,425 + -0.9 1,257,425 + +1.4 1,257,425 + +0.4 1,257,425 + +1.1 1,257,427 + +0.4 1,257,427 + +1.4 1,257,430 - -0.9 1,257,483 + -1.9 1,257,555 - MMJJ_RS06805 0.23 -0.6 1,257,556 + MMJJ_RS06805 0.23 +0.4 1,257,556 + MMJJ_RS06805 0.23 -1.0 1,257,615 - MMJJ_RS06805 0.43 -0.2 1,257,724 + MMJJ_RS06805 0.79 -1.0 1,257,747 - MMJJ_RS06805 0.86 -1.7 1,257,764 - +0.8 1,257,764 - +3.3 1,257,768 - +0.6 1,257,769 + +1.7 1,257,785 - +1.8 1,257,808 + +1.2 1,257,841 - -1.6 1,257,859 - -1.0 1,257,859 - +0.7 1,257,860 + +1.5 1,257,860 + +0.4 1,257,945 - +1.6 1,257,945 - -0.2 1,257,946 + +3.0 1,257,950 + +3.8 1,257,952 - -0.5 1,257,953 + -3.0 1,257,953 + +1.5 1,257,988 - MMJJ_RS06810 0.12 -2.4 1,257,989 + MMJJ_RS06810 0.12 -2.4 1,257,993 - MMJJ_RS06810 0.13 +0.2 1,258,017 - MMJJ_RS06810 0.15 +1.2 1,258,018 + MMJJ_RS06810 0.15 -1.2 1,258,020 + MMJJ_RS06810 0.15 -1.1 1,258,039 - MMJJ_RS06810 0.16 -0.0 1,258,056 - MMJJ_RS06810 0.18 +0.7 1,258,056 - MMJJ_RS06810 0.18 +1.7 1,258,093 - MMJJ_RS06810 0.21 -0.0 1,258,127 - MMJJ_RS06810 0.24 +1.9 1,258,176 - MMJJ_RS06810 0.28 -1.6 1,258,177 + MMJJ_RS06810 0.28 +1.8 1,258,180 - MMJJ_RS06810 0.28 +0.6 1,258,180 - MMJJ_RS06810 0.28 -1.5 1,258,203 - MMJJ_RS06810 0.30 +5.1 1,258,211 + MMJJ_RS06810 0.31 -1.4 1,258,217 + MMJJ_RS06810 0.31 -1.2 1,258,260 - MMJJ_RS06810 0.35 +2.2 1,258,270 + MMJJ_RS06810 0.36 -1.2 1,258,295 + MMJJ_RS06810 0.38 +5.9 1,258,309 + MMJJ_RS06810 0.39 +0.4 1,258,359 - MMJJ_RS06810 0.43 -3.5 1,258,360 + MMJJ_RS06810 0.43 +0.6 1,258,364 - MMJJ_RS06810 0.44 -0.2 1,258,364 - MMJJ_RS06810 0.44 +1.7 1,258,364 - MMJJ_RS06810 0.44 +3.6 1,258,365 + MMJJ_RS06810 0.44 -2.2 1,258,365 + MMJJ_RS06810 0.44 -0.2 1,258,365 + MMJJ_RS06810 0.44 -0.5 1,258,416 + MMJJ_RS06810 0.48 +2.2 1,258,416 + MMJJ_RS06810 0.48 +1.0 1,258,417 - MMJJ_RS06810 0.48 -0.6 1,258,418 + MMJJ_RS06810 0.48 -0.4 1,258,424 + MMJJ_RS06810 0.49 -0.2 1,258,432 + MMJJ_RS06810 0.49 +1.0 1,258,454 - MMJJ_RS06810 0.51 -1.2 1,258,486 - MMJJ_RS06810 0.54 -0.5 1,258,486 - MMJJ_RS06810 0.54 -2.9 1,258,486 - MMJJ_RS06810 0.54 +0.8 1,258,487 + MMJJ_RS06810 0.54 -1.8 1,258,528 - MMJJ_RS06810 0.57 -0.3 1,258,529 + MMJJ_RS06810 0.58 +4.0 1,258,529 + MMJJ_RS06810 0.58 +1.4 1,258,559 + MMJJ_RS06810 0.60 -0.6 1,258,559 + MMJJ_RS06810 0.60 +0.8 1,258,559 + MMJJ_RS06810 0.60 -0.2 1,258,587 - MMJJ_RS06810 0.62 +0.8 1,258,596 + MMJJ_RS06810 0.63 -0.9 1,258,630 + MMJJ_RS06810 0.66 -0.7 1,258,667 - MMJJ_RS06810 0.69 -1.0 1,258,678 - MMJJ_RS06810 0.70 -0.5 1,258,678 - MMJJ_RS06810 0.70 +3.2 1,258,679 + MMJJ_RS06810 0.70 +2.3 1,258,679 + MMJJ_RS06810 0.70 +3.8 1,258,679 + MMJJ_RS06810 0.70 +1.9 1,258,679 + MMJJ_RS06810 0.70 -0.9 1,258,688 - MMJJ_RS06810 0.71 -0.9 1,258,718 + MMJJ_RS06810 0.73 +1.6 1,258,718 + MMJJ_RS06810 0.73 -1.2 1,258,718 + MMJJ_RS06810 0.73 -1.0 1,258,737 - MMJJ_RS06810 0.75 +2.3
Or see this region's nucleotide sequence