Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06655 and MMJJ_RS06660 are separated by 6 nucleotides MMJJ_RS06660 and MMJJ_RS06665 overlap by 4 nucleotides MMJJ_RS06665 and MMJJ_RS06670 are separated by 24 nucleotides
MMJJ_RS06655: MMJJ_RS06655 - transcriptional regulator, at 1,227,451 to 1,227,963
_RS06655
MMJJ_RS06660: MMJJ_RS06660 - DUF1188 domain-containing protein, at 1,227,970 to 1,228,752
_RS06660
MMJJ_RS06665: MMJJ_RS06665 - DUF3236 domain-containing protein, at 1,228,749 to 1,229,228
_RS06665
MMJJ_RS06670: MMJJ_RS06670 - phenylalanine--tRNA ligase subunit alpha, at 1,229,253 to 1,230,758
_RS06670
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1227.799 kb on + strand, within MMJJ_RS06655 at 1227.812 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.858 kb on + strand, within MMJJ_RS06655 at 1227.861 kb on + strand, within MMJJ_RS06655 at 1227.864 kb on - strand, within MMJJ_RS06655 at 1227.884 kb on - strand, within MMJJ_RS06655 at 1227.885 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.907 kb on + strand, within MMJJ_RS06655 at 1227.972 kb on + strand at 1227.994 kb on - strand at 1227.994 kb on - strand at 1227.995 kb on + strand at 1227.995 kb on + strand at 1228.005 kb on - strand at 1228.019 kb on + strand at 1228.064 kb on + strand, within MMJJ_RS06660 at 1228.133 kb on - strand, within MMJJ_RS06660 at 1228.134 kb on + strand, within MMJJ_RS06660 at 1228.164 kb on - strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.201 kb on - strand, within MMJJ_RS06660 at 1228.312 kb on - strand, within MMJJ_RS06660 at 1228.410 kb on - strand, within MMJJ_RS06660 at 1228.510 kb on - strand, within MMJJ_RS06660 at 1228.510 kb on - strand, within MMJJ_RS06660 at 1228.516 kb on + strand, within MMJJ_RS06660 at 1228.522 kb on - strand, within MMJJ_RS06660 at 1228.541 kb on + strand, within MMJJ_RS06660 at 1228.549 kb on + strand, within MMJJ_RS06660 at 1228.567 kb on - strand, within MMJJ_RS06660 at 1228.587 kb on + strand, within MMJJ_RS06660 at 1228.601 kb on - strand, within MMJJ_RS06660 at 1228.626 kb on - strand, within MMJJ_RS06660 at 1228.642 kb on - strand, within MMJJ_RS06660 at 1228.643 kb on + strand, within MMJJ_RS06660 at 1228.643 kb on + strand, within MMJJ_RS06660 at 1228.649 kb on - strand, within MMJJ_RS06660 at 1228.766 kb on - strand at 1228.767 kb on + strand at 1228.797 kb on - strand, within MMJJ_RS06665 at 1228.932 kb on - strand, within MMJJ_RS06665 at 1228.933 kb on + strand, within MMJJ_RS06665 at 1228.981 kb on + strand, within MMJJ_RS06665 at 1228.998 kb on - strand, within MMJJ_RS06665 at 1229.035 kb on + strand, within MMJJ_RS06665 at 1229.036 kb on - strand, within MMJJ_RS06665 at 1229.036 kb on - strand, within MMJJ_RS06665 at 1229.060 kb on - strand, within MMJJ_RS06665 at 1229.109 kb on - strand, within MMJJ_RS06665 at 1229.157 kb on - strand, within MMJJ_RS06665 at 1229.177 kb on - strand, within MMJJ_RS06665 at 1229.178 kb on + strand, within MMJJ_RS06665 at 1229.197 kb on - strand at 1229.213 kb on - strand at 1229.254 kb on - strand at 1229.501 kb on + strand at 1229.655 kb on + strand at 1229.994 kb on - strand, within MMJJ_RS06670
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,227,799 + MMJJ_RS06655 0.68 +2.3 1,227,812 - MMJJ_RS06655 0.70 -0.1 1,227,814 - MMJJ_RS06655 0.71 +1.0 1,227,814 - MMJJ_RS06655 0.71 +3.4 1,227,814 - MMJJ_RS06655 0.71 +1.8 1,227,858 + MMJJ_RS06655 0.79 -1.8 1,227,861 + MMJJ_RS06655 0.80 -2.1 1,227,864 - MMJJ_RS06655 0.81 -2.7 1,227,884 - MMJJ_RS06655 0.84 +0.4 1,227,885 + MMJJ_RS06655 0.85 +0.0 1,227,891 + MMJJ_RS06655 0.86 +0.6 1,227,891 + MMJJ_RS06655 0.86 +1.3 1,227,907 + MMJJ_RS06655 0.89 -2.0 1,227,972 + -1.4 1,227,994 - -1.9 1,227,994 - -0.7 1,227,995 + -3.0 1,227,995 + -2.9 1,228,005 - -2.7 1,228,019 + +0.9 1,228,064 + MMJJ_RS06660 0.12 -0.6 1,228,133 - MMJJ_RS06660 0.21 -2.5 1,228,134 + MMJJ_RS06660 0.21 +0.3 1,228,164 - MMJJ_RS06660 0.25 -1.2 1,228,169 + MMJJ_RS06660 0.25 +0.8 1,228,169 + MMJJ_RS06660 0.25 -1.4 1,228,169 + MMJJ_RS06660 0.25 +0.1 1,228,201 - MMJJ_RS06660 0.30 -2.3 1,228,312 - MMJJ_RS06660 0.44 -1.0 1,228,410 - MMJJ_RS06660 0.56 -2.2 1,228,510 - MMJJ_RS06660 0.69 -3.0 1,228,510 - MMJJ_RS06660 0.69 -2.5 1,228,516 + MMJJ_RS06660 0.70 -2.2 1,228,522 - MMJJ_RS06660 0.70 -0.6 1,228,541 + MMJJ_RS06660 0.73 -0.7 1,228,549 + MMJJ_RS06660 0.74 -1.6 1,228,567 - MMJJ_RS06660 0.76 -2.7 1,228,587 + MMJJ_RS06660 0.79 -1.4 1,228,601 - MMJJ_RS06660 0.81 -1.8 1,228,626 - MMJJ_RS06660 0.84 -3.2 1,228,642 - MMJJ_RS06660 0.86 -0.6 1,228,643 + MMJJ_RS06660 0.86 -3.1 1,228,643 + MMJJ_RS06660 0.86 -2.8 1,228,649 - MMJJ_RS06660 0.87 -2.8 1,228,766 - -0.9 1,228,767 + -2.6 1,228,797 - MMJJ_RS06665 0.10 -0.9 1,228,932 - MMJJ_RS06665 0.38 -3.0 1,228,933 + MMJJ_RS06665 0.38 -3.0 1,228,981 + MMJJ_RS06665 0.48 -1.6 1,228,998 - MMJJ_RS06665 0.52 -0.6 1,229,035 + MMJJ_RS06665 0.60 +0.4 1,229,036 - MMJJ_RS06665 0.60 -2.2 1,229,036 - MMJJ_RS06665 0.60 -2.3 1,229,060 - MMJJ_RS06665 0.65 -1.6 1,229,109 - MMJJ_RS06665 0.75 -2.9 1,229,157 - MMJJ_RS06665 0.85 -0.9 1,229,177 - MMJJ_RS06665 0.89 -1.8 1,229,178 + MMJJ_RS06665 0.89 -1.2 1,229,197 - -3.4 1,229,213 - -1.4 1,229,254 - -1.0 1,229,501 + +0.4 1,229,655 + -0.6 1,229,994 - MMJJ_RS06670 0.49 -0.9
Or see this region's nucleotide sequence