Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06640 and MMJJ_RS06650 are separated by 568 nucleotides MMJJ_RS06650 and MMJJ_RS06655 are separated by 48 nucleotides MMJJ_RS06655 and MMJJ_RS06660 are separated by 6 nucleotides
MMJJ_RS06640: MMJJ_RS06640 - YfcE family phosphodiesterase, at 1,225,245 to 1,225,736
_RS06640
MMJJ_RS06650: MMJJ_RS06650 - cell division protein FtsZ, at 1,226,305 to 1,227,402
_RS06650
MMJJ_RS06655: MMJJ_RS06655 - transcriptional regulator, at 1,227,451 to 1,227,963
_RS06655
MMJJ_RS06660: MMJJ_RS06660 - DUF1188 domain-containing protein, at 1,227,970 to 1,228,752
_RS06660
Position (kb)
1226
1227
1228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1225.357 kb on + strand, within MMJJ_RS06640 at 1225.377 kb on - strand, within MMJJ_RS06640 at 1225.377 kb on - strand, within MMJJ_RS06640 at 1225.377 kb on - strand at 1225.378 kb on + strand, within MMJJ_RS06640 at 1225.378 kb on + strand, within MMJJ_RS06640 at 1225.378 kb on + strand, within MMJJ_RS06640 at 1225.386 kb on - strand, within MMJJ_RS06640 at 1225.386 kb on - strand, within MMJJ_RS06640 at 1225.386 kb on - strand, within MMJJ_RS06640 at 1225.391 kb on - strand, within MMJJ_RS06640 at 1225.391 kb on - strand, within MMJJ_RS06640 at 1225.391 kb on - strand, within MMJJ_RS06640 at 1225.392 kb on + strand, within MMJJ_RS06640 at 1225.392 kb on + strand, within MMJJ_RS06640 at 1225.403 kb on - strand, within MMJJ_RS06640 at 1225.404 kb on + strand, within MMJJ_RS06640 at 1225.404 kb on + strand, within MMJJ_RS06640 at 1225.444 kb on + strand, within MMJJ_RS06640 at 1225.448 kb on + strand, within MMJJ_RS06640 at 1225.463 kb on - strand, within MMJJ_RS06640 at 1225.463 kb on - strand, within MMJJ_RS06640 at 1225.463 kb on - strand, within MMJJ_RS06640 at 1225.464 kb on + strand, within MMJJ_RS06640 at 1225.464 kb on + strand, within MMJJ_RS06640 at 1225.464 kb on + strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.550 kb on - strand, within MMJJ_RS06640 at 1225.552 kb on - strand, within MMJJ_RS06640 at 1225.552 kb on - strand, within MMJJ_RS06640 at 1225.553 kb on + strand, within MMJJ_RS06640 at 1225.623 kb on - strand, within MMJJ_RS06640 at 1225.624 kb on + strand, within MMJJ_RS06640 at 1225.707 kb on + strand at 1225.728 kb on - strand at 1225.728 kb on - strand at 1225.730 kb on - strand at 1225.770 kb on - strand at 1225.771 kb on + strand at 1225.774 kb on - strand at 1226.117 kb on + strand at 1226.125 kb on + strand at 1226.127 kb on + strand at 1226.134 kb on - strand at 1226.134 kb on - strand at 1226.139 kb on - strand at 1226.140 kb on + strand at 1226.144 kb on - strand at 1226.148 kb on + strand at 1226.148 kb on + strand at 1226.154 kb on - strand at 1226.155 kb on + strand at 1226.158 kb on - strand at 1226.158 kb on - strand at 1226.160 kb on - strand at 1226.161 kb on + strand at 1226.161 kb on + strand at 1226.163 kb on - strand at 1226.163 kb on - strand at 1226.188 kb on - strand at 1226.188 kb on - strand at 1226.188 kb on - strand at 1226.196 kb on + strand at 1226.198 kb on - strand at 1226.199 kb on + strand at 1226.199 kb on + strand at 1226.201 kb on + strand at 1226.201 kb on + strand at 1226.201 kb on + strand at 1226.202 kb on - strand at 1226.202 kb on - strand at 1226.203 kb on + strand at 1226.207 kb on - strand at 1226.221 kb on - strand at 1226.221 kb on - strand at 1226.222 kb on + strand at 1226.225 kb on - strand at 1226.254 kb on - strand at 1226.254 kb on - strand at 1226.255 kb on + strand at 1226.257 kb on - strand at 1226.257 kb on - strand at 1226.258 kb on + strand at 1226.260 kb on - strand at 1226.267 kb on + strand at 1226.267 kb on + strand at 1226.272 kb on + strand at 1226.272 kb on + strand at 1226.301 kb on - strand at 1226.304 kb on - strand at 1226.304 kb on - strand at 1226.315 kb on - strand at 1226.315 kb on - strand at 1226.350 kb on - strand at 1226.350 kb on - strand at 1226.351 kb on + strand at 1226.351 kb on + strand at 1226.351 kb on + strand at 1226.427 kb on - strand, within MMJJ_RS06650 at 1226.454 kb on - strand, within MMJJ_RS06650 at 1226.455 kb on + strand, within MMJJ_RS06650 at 1226.481 kb on - strand, within MMJJ_RS06650 at 1226.485 kb on + strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.502 kb on + strand, within MMJJ_RS06650 at 1226.510 kb on - strand, within MMJJ_RS06650 at 1226.531 kb on - strand, within MMJJ_RS06650 at 1226.645 kb on - strand, within MMJJ_RS06650 at 1226.703 kb on + strand, within MMJJ_RS06650 at 1226.732 kb on - strand, within MMJJ_RS06650 at 1226.733 kb on + strand, within MMJJ_RS06650 at 1226.733 kb on + strand, within MMJJ_RS06650 at 1226.753 kb on + strand, within MMJJ_RS06650 at 1226.803 kb on + strand, within MMJJ_RS06650 at 1226.811 kb on - strand, within MMJJ_RS06650 at 1226.811 kb on - strand, within MMJJ_RS06650 at 1226.812 kb on + strand, within MMJJ_RS06650 at 1226.826 kb on - strand, within MMJJ_RS06650 at 1226.832 kb on - strand, within MMJJ_RS06650 at 1226.832 kb on - strand, within MMJJ_RS06650 at 1226.837 kb on - strand, within MMJJ_RS06650 at 1226.837 kb on - strand, within MMJJ_RS06650 at 1226.837 kb on - strand, within MMJJ_RS06650 at 1226.876 kb on - strand, within MMJJ_RS06650 at 1226.876 kb on - strand, within MMJJ_RS06650 at 1226.877 kb on + strand, within MMJJ_RS06650 at 1226.900 kb on - strand, within MMJJ_RS06650 at 1226.900 kb on - strand, within MMJJ_RS06650 at 1226.914 kb on + strand, within MMJJ_RS06650 at 1227.043 kb on - strand, within MMJJ_RS06650 at 1227.080 kb on - strand, within MMJJ_RS06650 at 1227.085 kb on + strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.090 kb on + strand, within MMJJ_RS06650 at 1227.090 kb on + strand, within MMJJ_RS06650 at 1227.104 kb on - strand, within MMJJ_RS06650 at 1227.104 kb on - strand, within MMJJ_RS06650 at 1227.104 kb on - strand, within MMJJ_RS06650 at 1227.140 kb on - strand, within MMJJ_RS06650 at 1227.140 kb on - strand, within MMJJ_RS06650 at 1227.141 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.164 kb on - strand, within MMJJ_RS06650 at 1227.180 kb on - strand, within MMJJ_RS06650 at 1227.180 kb on - strand, within MMJJ_RS06650 at 1227.180 kb on - strand, within MMJJ_RS06650 at 1227.219 kb on + strand, within MMJJ_RS06650 at 1227.231 kb on - strand, within MMJJ_RS06650 at 1227.231 kb on - strand, within MMJJ_RS06650 at 1227.288 kb on - strand, within MMJJ_RS06650 at 1227.291 kb on - strand, within MMJJ_RS06650 at 1227.312 kb on - strand at 1227.313 kb on + strand at 1227.314 kb on - strand at 1227.392 kb on + strand at 1227.392 kb on + strand at 1227.393 kb on - strand at 1227.394 kb on + strand at 1227.399 kb on + strand at 1227.399 kb on + strand at 1227.399 kb on + strand at 1227.432 kb on - strand at 1227.439 kb on - strand at 1227.440 kb on + strand at 1227.450 kb on - strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.492 kb on - strand at 1227.543 kb on + strand, within MMJJ_RS06655 at 1227.561 kb on + strand, within MMJJ_RS06655 at 1227.561 kb on + strand, within MMJJ_RS06655 at 1227.576 kb on - strand, within MMJJ_RS06655 at 1227.577 kb on + strand, within MMJJ_RS06655 at 1227.577 kb on + strand, within MMJJ_RS06655 at 1227.577 kb on + strand, within MMJJ_RS06655 at 1227.580 kb on + strand, within MMJJ_RS06655 at 1227.586 kb on - strand, within MMJJ_RS06655 at 1227.586 kb on - strand, within MMJJ_RS06655 at 1227.589 kb on + strand, within MMJJ_RS06655 at 1227.637 kb on + strand, within MMJJ_RS06655 at 1227.682 kb on + strand, within MMJJ_RS06655 at 1227.686 kb on - strand, within MMJJ_RS06655 at 1227.687 kb on + strand, within MMJJ_RS06655 at 1227.687 kb on + strand, within MMJJ_RS06655 at 1227.713 kb on - strand at 1227.723 kb on + strand, within MMJJ_RS06655 at 1227.799 kb on + strand, within MMJJ_RS06655 at 1227.812 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.858 kb on + strand, within MMJJ_RS06655 at 1227.861 kb on + strand, within MMJJ_RS06655 at 1227.864 kb on - strand, within MMJJ_RS06655 at 1227.884 kb on - strand, within MMJJ_RS06655 at 1227.885 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.907 kb on + strand, within MMJJ_RS06655 at 1227.972 kb on + strand at 1227.994 kb on - strand at 1227.994 kb on - strand at 1227.995 kb on + strand at 1227.995 kb on + strand at 1228.005 kb on - strand at 1228.019 kb on + strand at 1228.064 kb on + strand, within MMJJ_RS06660 at 1228.133 kb on - strand, within MMJJ_RS06660 at 1228.134 kb on + strand, within MMJJ_RS06660 at 1228.164 kb on - strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.201 kb on - strand, within MMJJ_RS06660 at 1228.312 kb on - strand, within MMJJ_RS06660
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,225,357 + MMJJ_RS06640 0.23 +1.7 1,225,377 - MMJJ_RS06640 0.27 +1.1 1,225,377 - MMJJ_RS06640 0.27 +0.4 1,225,377 - +1.4 1,225,378 + MMJJ_RS06640 0.27 -1.7 1,225,378 + MMJJ_RS06640 0.27 +1.8 1,225,378 + MMJJ_RS06640 0.27 +0.5 1,225,386 - MMJJ_RS06640 0.29 -1.0 1,225,386 - MMJJ_RS06640 0.29 -0.4 1,225,386 - MMJJ_RS06640 0.29 -0.1 1,225,391 - MMJJ_RS06640 0.30 +2.2 1,225,391 - MMJJ_RS06640 0.30 -1.0 1,225,391 - MMJJ_RS06640 0.30 +0.5 1,225,392 + MMJJ_RS06640 0.30 -0.9 1,225,392 + MMJJ_RS06640 0.30 +3.3 1,225,403 - MMJJ_RS06640 0.32 -1.6 1,225,404 + MMJJ_RS06640 0.32 -0.3 1,225,404 + MMJJ_RS06640 0.32 -2.3 1,225,444 + MMJJ_RS06640 0.40 +0.0 1,225,448 + MMJJ_RS06640 0.41 +0.2 1,225,463 - MMJJ_RS06640 0.44 +3.4 1,225,463 - MMJJ_RS06640 0.44 -0.1 1,225,463 - MMJJ_RS06640 0.44 +0.6 1,225,464 + MMJJ_RS06640 0.45 +1.0 1,225,464 + MMJJ_RS06640 0.45 -0.9 1,225,464 + MMJJ_RS06640 0.45 -1.6 1,225,524 - MMJJ_RS06640 0.57 -0.6 1,225,524 - MMJJ_RS06640 0.57 +2.4 1,225,524 - MMJJ_RS06640 0.57 -1.4 1,225,524 - MMJJ_RS06640 0.57 +1.7 1,225,550 - MMJJ_RS06640 0.62 -1.6 1,225,552 - MMJJ_RS06640 0.62 -0.1 1,225,552 - MMJJ_RS06640 0.62 +0.4 1,225,553 + MMJJ_RS06640 0.63 -1.3 1,225,623 - MMJJ_RS06640 0.77 -1.6 1,225,624 + MMJJ_RS06640 0.77 +1.8 1,225,707 + +3.1 1,225,728 - -2.0 1,225,728 - -2.0 1,225,730 - -1.7 1,225,770 - -0.7 1,225,771 + +2.4 1,225,774 - -1.0 1,226,117 + -0.6 1,226,125 + -1.7 1,226,127 + -0.1 1,226,134 - +0.4 1,226,134 - -0.1 1,226,139 - +4.5 1,226,140 + +4.4 1,226,144 - -0.2 1,226,148 + -2.2 1,226,148 + -1.7 1,226,154 - +0.4 1,226,155 + -0.6 1,226,158 - +0.6 1,226,158 - +0.2 1,226,160 - -0.6 1,226,161 + -2.2 1,226,161 + +1.4 1,226,163 - +1.4 1,226,163 - -0.1 1,226,188 - +1.2 1,226,188 - -3.3 1,226,188 - -0.7 1,226,196 + -1.6 1,226,198 - +3.0 1,226,199 + +3.7 1,226,199 + +0.6 1,226,201 + -0.1 1,226,201 + +3.7 1,226,201 + -2.0 1,226,202 - +3.8 1,226,202 - -1.6 1,226,203 + +1.8 1,226,207 - -3.2 1,226,221 - -0.1 1,226,221 - +0.5 1,226,222 + -2.8 1,226,225 - +1.8 1,226,254 - -2.1 1,226,254 - -0.6 1,226,255 + +0.6 1,226,257 - +0.8 1,226,257 - +3.5 1,226,258 + -1.4 1,226,260 - -0.1 1,226,267 + +2.5 1,226,267 + +2.8 1,226,272 + +0.1 1,226,272 + -1.4 1,226,301 - +0.1 1,226,304 - -1.1 1,226,304 - -1.8 1,226,315 - -0.6 1,226,315 - +2.5 1,226,350 - +2.1 1,226,350 - +0.1 1,226,351 + +3.7 1,226,351 + +0.4 1,226,351 + +0.7 1,226,427 - MMJJ_RS06650 0.11 -1.0 1,226,454 - MMJJ_RS06650 0.14 +1.1 1,226,455 + MMJJ_RS06650 0.14 +3.1 1,226,481 - MMJJ_RS06650 0.16 +0.2 1,226,485 + MMJJ_RS06650 0.16 -1.2 1,226,501 - MMJJ_RS06650 0.18 +1.5 1,226,501 - MMJJ_RS06650 0.18 +0.0 1,226,501 - MMJJ_RS06650 0.18 -2.0 1,226,501 - MMJJ_RS06650 0.18 +2.9 1,226,502 + MMJJ_RS06650 0.18 +1.8 1,226,510 - MMJJ_RS06650 0.19 -2.7 1,226,531 - MMJJ_RS06650 0.21 +1.2 1,226,645 - MMJJ_RS06650 0.31 +1.0 1,226,703 + MMJJ_RS06650 0.36 +1.8 1,226,732 - MMJJ_RS06650 0.39 +0.3 1,226,733 + MMJJ_RS06650 0.39 -1.7 1,226,733 + MMJJ_RS06650 0.39 +5.0 1,226,753 + MMJJ_RS06650 0.41 +2.5 1,226,803 + MMJJ_RS06650 0.45 -0.9 1,226,811 - MMJJ_RS06650 0.46 +2.1 1,226,811 - MMJJ_RS06650 0.46 -2.0 1,226,812 + MMJJ_RS06650 0.46 -0.1 1,226,826 - MMJJ_RS06650 0.47 +2.3 1,226,832 - MMJJ_RS06650 0.48 +5.3 1,226,832 - MMJJ_RS06650 0.48 -1.5 1,226,837 - MMJJ_RS06650 0.48 +1.3 1,226,837 - MMJJ_RS06650 0.48 -1.4 1,226,837 - MMJJ_RS06650 0.48 -0.5 1,226,876 - MMJJ_RS06650 0.52 +2.0 1,226,876 - MMJJ_RS06650 0.52 +3.9 1,226,877 + MMJJ_RS06650 0.52 +2.1 1,226,900 - MMJJ_RS06650 0.54 +1.9 1,226,900 - MMJJ_RS06650 0.54 +4.3 1,226,914 + MMJJ_RS06650 0.55 +0.0 1,227,043 - MMJJ_RS06650 0.67 +2.4 1,227,080 - MMJJ_RS06650 0.71 +5.6 1,227,085 + MMJJ_RS06650 0.71 -1.1 1,227,089 - MMJJ_RS06650 0.71 +1.9 1,227,089 - MMJJ_RS06650 0.71 +2.0 1,227,089 - MMJJ_RS06650 0.71 -2.1 1,227,089 - MMJJ_RS06650 0.71 +3.3 1,227,090 + MMJJ_RS06650 0.71 -0.9 1,227,090 + MMJJ_RS06650 0.71 +1.6 1,227,104 - MMJJ_RS06650 0.73 +0.8 1,227,104 - MMJJ_RS06650 0.73 -0.9 1,227,104 - MMJJ_RS06650 0.73 +1.9 1,227,140 - MMJJ_RS06650 0.76 -2.0 1,227,140 - MMJJ_RS06650 0.76 -1.9 1,227,141 + MMJJ_RS06650 0.76 -3.0 1,227,147 + MMJJ_RS06650 0.77 +2.7 1,227,147 + MMJJ_RS06650 0.77 -0.9 1,227,147 + MMJJ_RS06650 0.77 -1.4 1,227,147 + MMJJ_RS06650 0.77 +0.9 1,227,147 + MMJJ_RS06650 0.77 +1.9 1,227,164 - MMJJ_RS06650 0.78 +2.0 1,227,180 - MMJJ_RS06650 0.80 +3.1 1,227,180 - MMJJ_RS06650 0.80 -0.3 1,227,180 - MMJJ_RS06650 0.80 -2.0 1,227,219 + MMJJ_RS06650 0.83 -1.4 1,227,231 - MMJJ_RS06650 0.84 +3.2 1,227,231 - MMJJ_RS06650 0.84 +1.1 1,227,288 - MMJJ_RS06650 0.90 -2.4 1,227,291 - MMJJ_RS06650 0.90 -1.9 1,227,312 - -0.2 1,227,313 + -1.3 1,227,314 - +4.6 1,227,392 + +0.8 1,227,392 + +1.1 1,227,393 - +3.4 1,227,394 + -1.0 1,227,399 + -1.7 1,227,399 + +2.0 1,227,399 + +5.1 1,227,432 - -1.1 1,227,439 - +1.7 1,227,440 + +0.7 1,227,450 - -1.2 1,227,451 + +2.1 1,227,451 + +0.6 1,227,451 + +4.0 1,227,451 + -0.4 1,227,451 + +0.3 1,227,492 - +0.9 1,227,543 + MMJJ_RS06655 0.18 +5.1 1,227,561 + MMJJ_RS06655 0.21 +0.1 1,227,561 + MMJJ_RS06655 0.21 -1.3 1,227,576 - MMJJ_RS06655 0.24 +1.8 1,227,577 + MMJJ_RS06655 0.25 +2.3 1,227,577 + MMJJ_RS06655 0.25 +0.8 1,227,577 + MMJJ_RS06655 0.25 -2.3 1,227,580 + MMJJ_RS06655 0.25 +0.9 1,227,586 - MMJJ_RS06655 0.26 +1.1 1,227,586 - MMJJ_RS06655 0.26 +1.6 1,227,589 + MMJJ_RS06655 0.27 +1.7 1,227,637 + MMJJ_RS06655 0.36 +2.4 1,227,682 + MMJJ_RS06655 0.45 +1.3 1,227,686 - MMJJ_RS06655 0.46 +1.4 1,227,687 + MMJJ_RS06655 0.46 +1.2 1,227,687 + MMJJ_RS06655 0.46 -0.6 1,227,713 - +0.1 1,227,723 + MMJJ_RS06655 0.53 +0.0 1,227,799 + MMJJ_RS06655 0.68 +2.3 1,227,812 - MMJJ_RS06655 0.70 -0.1 1,227,814 - MMJJ_RS06655 0.71 +1.0 1,227,814 - MMJJ_RS06655 0.71 +3.4 1,227,814 - MMJJ_RS06655 0.71 +1.8 1,227,858 + MMJJ_RS06655 0.79 -1.8 1,227,861 + MMJJ_RS06655 0.80 -2.1 1,227,864 - MMJJ_RS06655 0.81 -2.7 1,227,884 - MMJJ_RS06655 0.84 +0.4 1,227,885 + MMJJ_RS06655 0.85 +0.0 1,227,891 + MMJJ_RS06655 0.86 +0.6 1,227,891 + MMJJ_RS06655 0.86 +1.3 1,227,907 + MMJJ_RS06655 0.89 -2.0 1,227,972 + -1.4 1,227,994 - -1.9 1,227,994 - -0.7 1,227,995 + -3.0 1,227,995 + -2.9 1,228,005 - -2.7 1,228,019 + +0.9 1,228,064 + MMJJ_RS06660 0.12 -0.6 1,228,133 - MMJJ_RS06660 0.21 -2.5 1,228,134 + MMJJ_RS06660 0.21 +0.3 1,228,164 - MMJJ_RS06660 0.25 -1.2 1,228,169 + MMJJ_RS06660 0.25 +0.8 1,228,169 + MMJJ_RS06660 0.25 -1.4 1,228,169 + MMJJ_RS06660 0.25 +0.1 1,228,201 - MMJJ_RS06660 0.30 -2.3 1,228,312 - MMJJ_RS06660 0.44 -1.0
Or see this region's nucleotide sequence