Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06490 and MMJJ_RS06495 are separated by 9 nucleotides MMJJ_RS06495 and MMJJ_RS06500 overlap by 4 nucleotides MMJJ_RS06500 and MMJJ_RS06505 overlap by 4 nucleotides
MMJJ_RS06490: MMJJ_RS06490 - methanogenesis marker 2 protein, at 1,197,534 to 1,198,505
_RS06490
MMJJ_RS06495: MMJJ_RS06495 - hypothetical protein, at 1,198,515 to 1,198,805
_RS06495
MMJJ_RS06500: MMJJ_RS06500 - class I SAM-dependent methyltransferase, at 1,198,802 to 1,199,512
_RS06500
MMJJ_RS06505: MMJJ_RS06505 - prephenate dehydratase, at 1,199,509 to 1,200,315
_RS06505
Position (kb)
1198
1199 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1197.647 kb on - strand, within MMJJ_RS06490 at 1197.656 kb on + strand, within MMJJ_RS06490 at 1197.836 kb on - strand, within MMJJ_RS06490 at 1197.847 kb on - strand, within MMJJ_RS06490 at 1197.962 kb on + strand at 1198.119 kb on - strand at 1198.181 kb on - strand, within MMJJ_RS06490 at 1198.189 kb on - strand, within MMJJ_RS06490 at 1198.265 kb on - strand at 1198.333 kb on - strand, within MMJJ_RS06490 at 1198.482 kb on + strand at 1198.488 kb on - strand at 1198.488 kb on - strand at 1198.488 kb on - strand at 1198.489 kb on + strand at 1198.489 kb on + strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.529 kb on + strand at 1198.538 kb on + strand at 1198.542 kb on - strand at 1198.559 kb on + strand, within MMJJ_RS06495 at 1198.665 kb on - strand, within MMJJ_RS06495 at 1198.711 kb on - strand, within MMJJ_RS06495 at 1198.726 kb on - strand, within MMJJ_RS06495 at 1198.751 kb on + strand, within MMJJ_RS06495 at 1198.772 kb on - strand, within MMJJ_RS06495 at 1198.787 kb on - strand at 1198.787 kb on - strand at 1198.827 kb on - strand at 1198.863 kb on + strand at 1198.868 kb on + strand at 1198.875 kb on - strand, within MMJJ_RS06500 at 1198.876 kb on + strand, within MMJJ_RS06500 at 1198.885 kb on + strand, within MMJJ_RS06500 at 1198.967 kb on + strand, within MMJJ_RS06500 at 1198.969 kb on - strand, within MMJJ_RS06500 at 1198.969 kb on - strand, within MMJJ_RS06500 at 1198.975 kb on + strand, within MMJJ_RS06500 at 1199.007 kb on + strand, within MMJJ_RS06500 at 1199.014 kb on + strand, within MMJJ_RS06500 at 1199.014 kb on + strand, within MMJJ_RS06500 at 1199.030 kb on + strand, within MMJJ_RS06500 at 1199.036 kb on - strand, within MMJJ_RS06500 at 1199.037 kb on + strand, within MMJJ_RS06500 at 1199.072 kb on + strand, within MMJJ_RS06500 at 1199.119 kb on - strand, within MMJJ_RS06500 at 1199.119 kb on - strand, within MMJJ_RS06500 at 1199.119 kb on - strand, within MMJJ_RS06500 at 1199.120 kb on + strand, within MMJJ_RS06500 at 1199.228 kb on - strand, within MMJJ_RS06500 at 1199.239 kb on + strand, within MMJJ_RS06500 at 1199.241 kb on - strand, within MMJJ_RS06500 at 1199.242 kb on + strand, within MMJJ_RS06500 at 1199.408 kb on + strand, within MMJJ_RS06500 at 1199.412 kb on - strand, within MMJJ_RS06500
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,197,647 - MMJJ_RS06490 0.12 -0.5 1,197,656 + MMJJ_RS06490 0.13 +0.5 1,197,836 - MMJJ_RS06490 0.31 +0.5 1,197,847 - MMJJ_RS06490 0.32 -0.1 1,197,962 + -0.1 1,198,119 - +0.5 1,198,181 - MMJJ_RS06490 0.67 -0.1 1,198,189 - MMJJ_RS06490 0.67 +0.5 1,198,265 - -0.1 1,198,333 - MMJJ_RS06490 0.82 +0.5 1,198,482 + -1.0 1,198,488 - -2.9 1,198,488 - -1.7 1,198,488 - -1.1 1,198,489 + -0.5 1,198,489 + -1.9 1,198,528 - -1.1 1,198,528 - -0.1 1,198,528 - -1.5 1,198,528 - -0.5 1,198,528 - +3.8 1,198,528 - +2.0 1,198,529 + -0.4 1,198,538 + +1.2 1,198,542 - -1.1 1,198,559 + MMJJ_RS06495 0.15 -0.4 1,198,665 - MMJJ_RS06495 0.52 -0.9 1,198,711 - MMJJ_RS06495 0.67 -2.4 1,198,726 - MMJJ_RS06495 0.73 -2.2 1,198,751 + MMJJ_RS06495 0.81 -2.1 1,198,772 - MMJJ_RS06495 0.88 -0.1 1,198,787 - +0.2 1,198,787 - -3.2 1,198,827 - +4.1 1,198,863 + -3.7 1,198,868 + +1.0 1,198,875 - MMJJ_RS06500 0.10 -0.1 1,198,876 + MMJJ_RS06500 0.10 -3.5 1,198,885 + MMJJ_RS06500 0.12 +1.0 1,198,967 + MMJJ_RS06500 0.23 +2.9 1,198,969 - MMJJ_RS06500 0.23 -1.4 1,198,969 - MMJJ_RS06500 0.23 +2.5 1,198,975 + MMJJ_RS06500 0.24 -2.5 1,199,007 + MMJJ_RS06500 0.29 +3.2 1,199,014 + MMJJ_RS06500 0.30 +1.8 1,199,014 + MMJJ_RS06500 0.30 -1.9 1,199,030 + MMJJ_RS06500 0.32 +2.5 1,199,036 - MMJJ_RS06500 0.33 +4.2 1,199,037 + MMJJ_RS06500 0.33 -0.9 1,199,072 + MMJJ_RS06500 0.38 -0.6 1,199,119 - MMJJ_RS06500 0.45 +2.7 1,199,119 - MMJJ_RS06500 0.45 -0.5 1,199,119 - MMJJ_RS06500 0.45 -1.7 1,199,120 + MMJJ_RS06500 0.45 -2.1 1,199,228 - MMJJ_RS06500 0.60 -0.0 1,199,239 + MMJJ_RS06500 0.61 +1.6 1,199,241 - MMJJ_RS06500 0.62 +2.3 1,199,242 + MMJJ_RS06500 0.62 +2.0 1,199,408 + MMJJ_RS06500 0.85 -0.5 1,199,412 - MMJJ_RS06500 0.86 +0.2
Or see this region's nucleotide sequence