Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09320 and MMJJ_RS06370 are separated by 129 nucleotides MMJJ_RS06370 and MMJJ_RS06375 are separated by 159 nucleotides MMJJ_RS06375 and MMJJ_RS06380 are separated by 26 nucleotides MMJJ_RS06380 and MMJJ_RS06385 are separated by 137 nucleotides MMJJ_RS06385 and MMJJ_RS06390 overlap by 4 nucleotides
MMJJ_RS09320: MMJJ_RS09320 - tRNA-Met, at 1,180,493 to 1,180,607
_RS09320
MMJJ_RS06370: MMJJ_RS06370 - tRNA-Leu, at 1,180,737 to 1,180,821
_RS06370
MMJJ_RS06375: MMJJ_RS06375 - UbiX family flavin prenyltransferase, at 1,180,981 to 1,181,538
_RS06375
MMJJ_RS06380: MMJJ_RS06380 - signal recognition particle protein Srp54, at 1,181,565 to 1,182,917
_RS06380
MMJJ_RS06385: MMJJ_RS06385 - hypothetical protein, at 1,183,055 to 1,183,276
_RS06385
MMJJ_RS06390: MMJJ_RS06390 - NADPH-dependent F420 reductase, at 1,183,273 to 1,183,944
_RS06390
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1180.651 kb on + strand at 1180.858 kb on + strand at 1180.879 kb on - strand at 1180.880 kb on + strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.889 kb on - strand at 1180.889 kb on - strand at 1180.896 kb on + strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.932 kb on - strand at 1180.932 kb on - strand at 1180.932 kb on - strand at 1180.933 kb on + strand at 1180.933 kb on + strand at 1180.933 kb on + strand at 1180.934 kb on - strand at 1180.934 kb on - strand at 1180.934 kb on - strand at 1180.935 kb on + strand at 1180.936 kb on - strand at 1180.937 kb on + strand at 1180.937 kb on + strand at 1180.937 kb on + strand at 1180.958 kb on + strand at 1180.961 kb on + strand at 1180.961 kb on + strand at 1180.961 kb on + strand at 1180.963 kb on + strand at 1180.980 kb on - strand at 1180.995 kb on + strand at 1180.995 kb on + strand at 1181.272 kb on - strand, within MMJJ_RS06375 at 1181.280 kb on - strand, within MMJJ_RS06375 at 1181.331 kb on + strand, within MMJJ_RS06375 at 1181.359 kb on + strand, within MMJJ_RS06375 at 1181.361 kb on - strand, within MMJJ_RS06375 at 1181.390 kb on - strand, within MMJJ_RS06375 at 1181.435 kb on + strand, within MMJJ_RS06375 at 1181.453 kb on - strand, within MMJJ_RS06375 at 1181.453 kb on - strand, within MMJJ_RS06375 at 1181.517 kb on + strand at 1181.520 kb on - strand at 1181.536 kb on - strand at 1181.536 kb on - strand at 1181.537 kb on + strand at 1181.537 kb on + strand at 1181.547 kb on - strand at 1181.550 kb on - strand at 1181.564 kb on - strand at 1181.876 kb on + strand, within MMJJ_RS06380 at 1181.934 kb on + strand, within MMJJ_RS06380 at 1181.995 kb on + strand, within MMJJ_RS06380 at 1182.005 kb on + strand, within MMJJ_RS06380 at 1182.218 kb on + strand, within MMJJ_RS06380 at 1182.293 kb on - strand, within MMJJ_RS06380 at 1182.311 kb on + strand, within MMJJ_RS06380 at 1182.359 kb on + strand, within MMJJ_RS06380 at 1182.359 kb on + strand, within MMJJ_RS06380 at 1182.461 kb on + strand, within MMJJ_RS06380 at 1182.491 kb on + strand, within MMJJ_RS06380 at 1182.908 kb on - strand at 1182.921 kb on + strand at 1182.932 kb on + strand at 1182.946 kb on - strand at 1182.967 kb on - strand at 1182.975 kb on + strand at 1182.975 kb on + strand at 1183.009 kb on + strand at 1183.013 kb on + strand at 1183.051 kb on - strand at 1183.063 kb on - strand at 1183.068 kb on - strand at 1183.083 kb on - strand, within MMJJ_RS06385 at 1183.084 kb on + strand, within MMJJ_RS06385 at 1183.129 kb on + strand, within MMJJ_RS06385 at 1183.135 kb on - strand, within MMJJ_RS06385 at 1183.135 kb on - strand, within MMJJ_RS06385 at 1183.137 kb on - strand, within MMJJ_RS06385 at 1183.137 kb on - strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.182 kb on - strand, within MMJJ_RS06385 at 1183.186 kb on + strand, within MMJJ_RS06385 at 1183.186 kb on + strand, within MMJJ_RS06385 at 1183.188 kb on - strand, within MMJJ_RS06385 at 1183.199 kb on + strand, within MMJJ_RS06385 at 1183.264 kb on + strand at 1183.274 kb on - strand at 1183.291 kb on + strand at 1183.504 kb on - strand, within MMJJ_RS06390 at 1183.707 kb on - strand, within MMJJ_RS06390 at 1183.735 kb on - strand, within MMJJ_RS06390 at 1183.777 kb on + strand, within MMJJ_RS06390
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,180,651 + +0.4 1,180,858 + +1.8 1,180,879 - -0.1 1,180,880 + -2.2 1,180,884 - +0.2 1,180,884 - +0.5 1,180,884 - +2.0 1,180,884 - +2.6 1,180,884 - -0.2 1,180,884 - +1.2 1,180,884 - -2.3 1,180,884 - +1.7 1,180,884 - +0.3 1,180,884 - -1.9 1,180,884 - +0.8 1,180,884 - +1.6 1,180,884 - +1.5 1,180,885 + -1.1 1,180,885 + -1.8 1,180,885 + -0.5 1,180,885 + +2.0 1,180,885 + -1.4 1,180,885 + +0.4 1,180,885 + +2.9 1,180,885 + +0.4 1,180,885 + -0.4 1,180,889 - +1.3 1,180,889 - +0.8 1,180,896 + -2.5 1,180,930 - +2.7 1,180,930 - +2.0 1,180,930 - +1.5 1,180,930 - -2.8 1,180,930 - -0.4 1,180,930 - +5.2 1,180,930 - -2.8 1,180,930 - -1.6 1,180,930 - +3.4 1,180,930 - -1.0 1,180,930 - -1.3 1,180,930 - -1.4 1,180,930 - +1.1 1,180,931 + -3.3 1,180,931 + -0.4 1,180,931 + -0.6 1,180,931 + -0.6 1,180,931 + +2.3 1,180,931 + -0.6 1,180,931 + +1.4 1,180,931 + -2.3 1,180,931 + +1.7 1,180,931 + -1.7 1,180,932 - -0.6 1,180,932 - +0.5 1,180,932 - -0.6 1,180,933 + +0.9 1,180,933 + +3.1 1,180,933 + +3.7 1,180,934 - -2.0 1,180,934 - -0.2 1,180,934 - -1.6 1,180,935 + -1.1 1,180,936 - -1.5 1,180,937 + +0.5 1,180,937 + -1.6 1,180,937 + -2.3 1,180,958 + +0.9 1,180,961 + +0.4 1,180,961 + +0.1 1,180,961 + -0.7 1,180,963 + -0.6 1,180,980 - +0.4 1,180,995 + -1.8 1,180,995 + -2.5 1,181,272 - MMJJ_RS06375 0.52 +0.4 1,181,280 - MMJJ_RS06375 0.54 -1.6 1,181,331 + MMJJ_RS06375 0.63 +0.1 1,181,359 + MMJJ_RS06375 0.68 -1.6 1,181,361 - MMJJ_RS06375 0.68 -1.4 1,181,390 - MMJJ_RS06375 0.73 +1.4 1,181,435 + MMJJ_RS06375 0.81 -2.0 1,181,453 - MMJJ_RS06375 0.85 -2.4 1,181,453 - MMJJ_RS06375 0.85 -1.9 1,181,517 + -0.9 1,181,520 - -0.1 1,181,536 - +2.6 1,181,536 - +2.3 1,181,537 + -2.0 1,181,537 + -1.7 1,181,547 - -1.6 1,181,550 - +1.7 1,181,564 - -0.9 1,181,876 + MMJJ_RS06380 0.23 +0.9 1,181,934 + MMJJ_RS06380 0.27 -0.9 1,181,995 + MMJJ_RS06380 0.32 -0.5 1,182,005 + MMJJ_RS06380 0.33 +0.5 1,182,218 + MMJJ_RS06380 0.48 -1.7 1,182,293 - MMJJ_RS06380 0.54 -1.4 1,182,311 + MMJJ_RS06380 0.55 +0.9 1,182,359 + MMJJ_RS06380 0.59 -0.1 1,182,359 + MMJJ_RS06380 0.59 -1.4 1,182,461 + MMJJ_RS06380 0.66 -0.9 1,182,491 + MMJJ_RS06380 0.68 -0.1 1,182,908 - +3.4 1,182,921 + +0.5 1,182,932 + -1.3 1,182,946 - -0.1 1,182,967 - -1.9 1,182,975 + -1.9 1,182,975 + -1.4 1,183,009 + +1.8 1,183,013 + +1.0 1,183,051 - -0.6 1,183,063 - +1.8 1,183,068 - +1.5 1,183,083 - MMJJ_RS06385 0.13 -0.5 1,183,084 + MMJJ_RS06385 0.13 -2.8 1,183,129 + MMJJ_RS06385 0.33 +0.5 1,183,135 - MMJJ_RS06385 0.36 +1.3 1,183,135 - MMJJ_RS06385 0.36 +2.9 1,183,137 - MMJJ_RS06385 0.37 -1.2 1,183,137 - MMJJ_RS06385 0.37 +2.7 1,183,138 + MMJJ_RS06385 0.37 -3.0 1,183,138 + MMJJ_RS06385 0.37 +1.3 1,183,138 + MMJJ_RS06385 0.37 +0.2 1,183,182 - MMJJ_RS06385 0.57 -0.3 1,183,186 + MMJJ_RS06385 0.59 -1.4 1,183,186 + MMJJ_RS06385 0.59 -2.7 1,183,188 - MMJJ_RS06385 0.60 +0.7 1,183,199 + MMJJ_RS06385 0.65 +0.6 1,183,264 + +4.0 1,183,274 - -0.6 1,183,291 + +1.5 1,183,504 - MMJJ_RS06390 0.34 -0.5 1,183,707 - MMJJ_RS06390 0.65 -0.1 1,183,735 - MMJJ_RS06390 0.69 -0.9 1,183,777 + MMJJ_RS06390 0.75 +1.5
Or see this region's nucleotide sequence