Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06265 and MMJJ_RS06270 are separated by 62 nucleotides MMJJ_RS06270 and MMJJ_RS06275 are separated by 11 nucleotides MMJJ_RS06275 and MMJJ_RS06280 are separated by 88 nucleotides
MMJJ_RS06265: MMJJ_RS06265 - 8-amino-7-oxononanoate synthase, at 1,163,365 to 1,164,477
_RS06265
MMJJ_RS06270: MMJJ_RS06270 - dethiobiotin synthase, at 1,164,540 to 1,165,112
_RS06270
MMJJ_RS06275: MMJJ_RS06275 - hypothetical protein, at 1,165,124 to 1,165,420
_RS06275
MMJJ_RS06280: MMJJ_RS06280 - hypothetical protein, at 1,165,509 to 1,166,444
_RS06280
Position (kb)
1164
1165
1166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.635 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.670 kb on + strand, within MMJJ_RS06265 at 1163.683 kb on + strand, within MMJJ_RS06265 at 1163.714 kb on - strand, within MMJJ_RS06265 at 1163.730 kb on + strand, within MMJJ_RS06265 at 1163.742 kb on + strand, within MMJJ_RS06265 at 1163.782 kb on + strand, within MMJJ_RS06265 at 1163.798 kb on - strand, within MMJJ_RS06265 at 1163.799 kb on + strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.961 kb on + strand, within MMJJ_RS06265 at 1164.013 kb on + strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.066 kb on + strand, within MMJJ_RS06265 at 1164.092 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.160 kb on + strand, within MMJJ_RS06265 at 1164.274 kb on + strand, within MMJJ_RS06265 at 1164.375 kb on - strand at 1164.420 kb on - strand at 1164.439 kb on - strand at 1164.480 kb on - strand at 1164.481 kb on + strand at 1164.487 kb on - strand at 1164.488 kb on + strand at 1164.525 kb on - strand at 1164.526 kb on + strand at 1164.526 kb on + strand at 1164.568 kb on - strand at 1164.569 kb on + strand at 1164.569 kb on + strand at 1164.581 kb on - strand at 1164.581 kb on - strand at 1164.582 kb on + strand at 1164.582 kb on + strand at 1164.584 kb on + strand at 1164.584 kb on + strand at 1164.615 kb on + strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.622 kb on + strand, within MMJJ_RS06270 at 1164.622 kb on + strand, within MMJJ_RS06270 at 1164.622 kb on + strand, within MMJJ_RS06270 at 1164.627 kb on + strand, within MMJJ_RS06270 at 1164.731 kb on + strand, within MMJJ_RS06270 at 1164.784 kb on + strand, within MMJJ_RS06270 at 1164.788 kb on + strand, within MMJJ_RS06270 at 1164.834 kb on - strand, within MMJJ_RS06270 at 1164.850 kb on - strand, within MMJJ_RS06270 at 1164.919 kb on - strand, within MMJJ_RS06270 at 1164.919 kb on - strand, within MMJJ_RS06270 at 1164.933 kb on - strand, within MMJJ_RS06270 at 1164.945 kb on + strand, within MMJJ_RS06270 at 1164.958 kb on - strand, within MMJJ_RS06270 at 1164.959 kb on + strand, within MMJJ_RS06270 at 1164.974 kb on + strand, within MMJJ_RS06270 at 1165.005 kb on - strand, within MMJJ_RS06270 at 1165.009 kb on + strand, within MMJJ_RS06270 at 1165.094 kb on - strand at 1165.094 kb on - strand at 1165.095 kb on + strand at 1165.095 kb on + strand at 1165.155 kb on + strand, within MMJJ_RS06275 at 1165.171 kb on - strand, within MMJJ_RS06275 at 1165.175 kb on - strand, within MMJJ_RS06275 at 1165.210 kb on - strand, within MMJJ_RS06275 at 1165.363 kb on - strand, within MMJJ_RS06275 at 1165.363 kb on - strand, within MMJJ_RS06275 at 1165.364 kb on + strand, within MMJJ_RS06275 at 1165.367 kb on - strand, within MMJJ_RS06275 at 1165.367 kb on - strand, within MMJJ_RS06275 at 1165.368 kb on + strand, within MMJJ_RS06275 at 1165.374 kb on + strand, within MMJJ_RS06275 at 1165.394 kb on - strand at 1165.440 kb on - strand at 1165.440 kb on - strand at 1165.440 kb on - strand at 1165.440 kb on - strand at 1165.441 kb on + strand at 1165.441 kb on + strand at 1165.442 kb on - strand at 1165.442 kb on - strand at 1165.443 kb on + strand at 1165.443 kb on + strand at 1165.448 kb on - strand at 1165.448 kb on - strand at 1165.449 kb on + strand at 1165.449 kb on + strand at 1165.449 kb on + strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.451 kb on + strand at 1165.451 kb on + strand at 1165.451 kb on + strand at 1165.451 kb on + strand at 1165.452 kb on - strand at 1165.452 kb on - strand at 1165.452 kb on - strand at 1165.453 kb on + strand at 1165.453 kb on + strand at 1165.453 kb on + strand at 1165.453 kb on + strand at 1165.454 kb on - strand at 1165.454 kb on - strand at 1165.454 kb on - strand at 1165.454 kb on - strand at 1165.455 kb on + strand at 1165.455 kb on + strand at 1165.455 kb on + strand at 1165.455 kb on + strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.482 kb on + strand at 1165.484 kb on + strand at 1165.484 kb on + strand at 1165.484 kb on + strand at 1165.484 kb on + strand at 1165.484 kb on + strand at 1165.488 kb on + strand at 1165.505 kb on - strand at 1165.515 kb on + strand at 1165.517 kb on - strand at 1165.517 kb on - strand at 1165.517 kb on - strand at 1165.518 kb on + strand at 1165.518 kb on + strand at 1165.554 kb on - strand at 1165.554 kb on - strand at 1165.579 kb on - strand at 1165.579 kb on - strand at 1165.580 kb on + strand at 1165.580 kb on + strand at 1165.593 kb on - strand at 1165.593 kb on - strand at 1165.594 kb on + strand at 1165.594 kb on + strand at 1165.601 kb on + strand at 1165.644 kb on - strand, within MMJJ_RS06280 at 1165.644 kb on - strand, within MMJJ_RS06280 at 1165.645 kb on + strand, within MMJJ_RS06280 at 1165.645 kb on + strand, within MMJJ_RS06280 at 1165.645 kb on + strand, within MMJJ_RS06280 at 1165.687 kb on - strand, within MMJJ_RS06280 at 1165.687 kb on - strand, within MMJJ_RS06280 at 1165.768 kb on - strand, within MMJJ_RS06280 at 1165.834 kb on - strand, within MMJJ_RS06280 at 1165.839 kb on - strand, within MMJJ_RS06280 at 1165.843 kb on + strand, within MMJJ_RS06280 at 1165.843 kb on + strand, within MMJJ_RS06280 at 1165.845 kb on + strand, within MMJJ_RS06280 at 1165.845 kb on + strand, within MMJJ_RS06280 at 1165.868 kb on + strand, within MMJJ_RS06280 at 1165.872 kb on - strand, within MMJJ_RS06280 at 1165.874 kb on - strand, within MMJJ_RS06280 at 1165.875 kb on + strand, within MMJJ_RS06280 at 1165.926 kb on + strand, within MMJJ_RS06280 at 1165.926 kb on + strand, within MMJJ_RS06280 at 1165.927 kb on - strand, within MMJJ_RS06280 at 1165.951 kb on - strand, within MMJJ_RS06280 at 1165.952 kb on + strand, within MMJJ_RS06280 at 1165.994 kb on - strand, within MMJJ_RS06280 at 1165.995 kb on + strand, within MMJJ_RS06280 at 1165.995 kb on + strand, within MMJJ_RS06280 at 1166.004 kb on + strand, within MMJJ_RS06280 at 1166.067 kb on + strand, within MMJJ_RS06280 at 1166.089 kb on + strand, within MMJJ_RS06280
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,163,574 + MMJJ_RS06265 0.19 -2.6 1,163,574 + MMJJ_RS06265 0.19 -2.3 1,163,574 + MMJJ_RS06265 0.19 -0.2 1,163,635 - MMJJ_RS06265 0.24 -0.9 1,163,660 - MMJJ_RS06265 0.27 -0.1 1,163,660 - MMJJ_RS06265 0.27 +0.2 1,163,667 - MMJJ_RS06265 0.27 -0.0 1,163,667 - MMJJ_RS06265 0.27 +1.4 1,163,667 - MMJJ_RS06265 0.27 -1.1 1,163,669 - MMJJ_RS06265 0.27 +1.4 1,163,669 - MMJJ_RS06265 0.27 -1.7 1,163,669 - MMJJ_RS06265 0.27 -3.1 1,163,669 - MMJJ_RS06265 0.27 +0.4 1,163,670 + MMJJ_RS06265 0.27 +0.9 1,163,683 + MMJJ_RS06265 0.29 -2.3 1,163,714 - MMJJ_RS06265 0.31 -1.3 1,163,730 + MMJJ_RS06265 0.33 -1.1 1,163,742 + MMJJ_RS06265 0.34 -2.4 1,163,782 + MMJJ_RS06265 0.37 -2.0 1,163,798 - MMJJ_RS06265 0.39 +0.4 1,163,799 + MMJJ_RS06265 0.39 -0.6 1,163,843 - MMJJ_RS06265 0.43 -1.4 1,163,843 - MMJJ_RS06265 0.43 -1.7 1,163,843 - MMJJ_RS06265 0.43 -1.1 1,163,843 - MMJJ_RS06265 0.43 -1.2 1,163,844 + MMJJ_RS06265 0.43 +0.4 1,163,844 + MMJJ_RS06265 0.43 -2.4 1,163,844 + MMJJ_RS06265 0.43 -1.1 1,163,914 - MMJJ_RS06265 0.49 +0.1 1,163,914 - MMJJ_RS06265 0.49 -1.6 1,163,928 + MMJJ_RS06265 0.51 -2.0 1,163,928 + MMJJ_RS06265 0.51 +1.4 1,163,961 + MMJJ_RS06265 0.54 -0.6 1,164,013 + MMJJ_RS06265 0.58 +0.5 1,164,065 - MMJJ_RS06265 0.63 -0.6 1,164,065 - MMJJ_RS06265 0.63 -1.4 1,164,065 - MMJJ_RS06265 0.63 -0.4 1,164,065 - MMJJ_RS06265 0.63 +1.6 1,164,066 + MMJJ_RS06265 0.63 -1.7 1,164,092 + MMJJ_RS06265 0.65 -1.2 1,164,118 + MMJJ_RS06265 0.68 -0.5 1,164,118 + MMJJ_RS06265 0.68 -1.7 1,164,160 + MMJJ_RS06265 0.71 -1.1 1,164,274 + MMJJ_RS06265 0.82 -2.6 1,164,375 - -1.7 1,164,420 - -0.1 1,164,439 - -1.9 1,164,480 - +0.4 1,164,481 + -0.9 1,164,487 - +1.4 1,164,488 + -1.1 1,164,525 - -1.6 1,164,526 + -1.2 1,164,526 + -0.9 1,164,568 - -1.0 1,164,569 + +1.4 1,164,569 + -2.1 1,164,581 - +0.4 1,164,581 - -0.9 1,164,582 + -0.4 1,164,582 + -0.6 1,164,584 + +1.9 1,164,584 + -1.4 1,164,615 + MMJJ_RS06270 0.13 -1.8 1,164,621 - MMJJ_RS06270 0.14 -0.6 1,164,621 - MMJJ_RS06270 0.14 -2.0 1,164,621 - MMJJ_RS06270 0.14 -1.7 1,164,621 - MMJJ_RS06270 0.14 -0.9 1,164,622 + MMJJ_RS06270 0.14 -0.1 1,164,622 + MMJJ_RS06270 0.14 -1.9 1,164,622 + MMJJ_RS06270 0.14 -1.4 1,164,627 + MMJJ_RS06270 0.15 -0.9 1,164,731 + MMJJ_RS06270 0.33 -2.5 1,164,784 + MMJJ_RS06270 0.43 +1.4 1,164,788 + MMJJ_RS06270 0.43 +0.0 1,164,834 - MMJJ_RS06270 0.51 +0.4 1,164,850 - MMJJ_RS06270 0.54 -2.4 1,164,919 - MMJJ_RS06270 0.66 -0.1 1,164,919 - MMJJ_RS06270 0.66 +0.4 1,164,933 - MMJJ_RS06270 0.69 -0.4 1,164,945 + MMJJ_RS06270 0.71 -0.9 1,164,958 - MMJJ_RS06270 0.73 +1.0 1,164,959 + MMJJ_RS06270 0.73 -1.9 1,164,974 + MMJJ_RS06270 0.76 -0.9 1,165,005 - MMJJ_RS06270 0.81 -2.3 1,165,009 + MMJJ_RS06270 0.82 -2.1 1,165,094 - -2.1 1,165,094 - +2.9 1,165,095 + +2.5 1,165,095 + +1.4 1,165,155 + MMJJ_RS06275 0.10 +0.4 1,165,171 - MMJJ_RS06275 0.16 +0.3 1,165,175 - MMJJ_RS06275 0.17 -0.8 1,165,210 - MMJJ_RS06275 0.29 +0.3 1,165,363 - MMJJ_RS06275 0.80 -1.8 1,165,363 - MMJJ_RS06275 0.80 -1.2 1,165,364 + MMJJ_RS06275 0.81 +0.2 1,165,367 - MMJJ_RS06275 0.82 +4.2 1,165,367 - MMJJ_RS06275 0.82 +1.2 1,165,368 + MMJJ_RS06275 0.82 +1.1 1,165,374 + MMJJ_RS06275 0.84 +1.1 1,165,394 - +2.1 1,165,440 - -0.8 1,165,440 - +1.3 1,165,440 - -2.4 1,165,440 - +2.7 1,165,441 + -1.2 1,165,441 + -0.2 1,165,442 - -2.5 1,165,442 - +3.5 1,165,443 + -2.6 1,165,443 + +2.9 1,165,448 - +2.7 1,165,448 - +1.4 1,165,449 + -1.9 1,165,449 + +2.3 1,165,449 + -3.1 1,165,450 - -1.1 1,165,450 - -2.0 1,165,450 - +1.3 1,165,450 - +1.7 1,165,450 - +1.4 1,165,451 + -2.0 1,165,451 + -2.8 1,165,451 + -1.1 1,165,451 + +1.7 1,165,452 - -1.6 1,165,452 - +1.8 1,165,452 - +0.1 1,165,453 + +0.2 1,165,453 + -0.1 1,165,453 + +1.4 1,165,453 + -0.9 1,165,454 - +3.5 1,165,454 - +1.9 1,165,454 - -0.7 1,165,454 - -0.9 1,165,455 + +2.7 1,165,455 + -1.7 1,165,455 + -1.0 1,165,455 + -2.3 1,165,458 - -2.8 1,165,458 - +0.9 1,165,458 - -0.1 1,165,458 - -1.5 1,165,458 - -1.0 1,165,482 + +1.4 1,165,484 + +0.2 1,165,484 + +0.9 1,165,484 + -0.0 1,165,484 + +2.1 1,165,484 + -2.0 1,165,488 + -2.8 1,165,505 - -0.0 1,165,515 + +1.1 1,165,517 - +0.4 1,165,517 - -0.1 1,165,517 - +3.3 1,165,518 + -0.8 1,165,518 + +1.5 1,165,554 - -1.9 1,165,554 - -0.8 1,165,579 - -0.1 1,165,579 - -0.1 1,165,580 + -0.5 1,165,580 + -0.1 1,165,593 - +2.5 1,165,593 - +0.7 1,165,594 + -1.6 1,165,594 + +1.7 1,165,601 + -0.4 1,165,644 - MMJJ_RS06280 0.14 -1.7 1,165,644 - MMJJ_RS06280 0.14 -1.3 1,165,645 + MMJJ_RS06280 0.15 +3.8 1,165,645 + MMJJ_RS06280 0.15 +1.4 1,165,645 + MMJJ_RS06280 0.15 +0.3 1,165,687 - MMJJ_RS06280 0.19 -2.7 1,165,687 - MMJJ_RS06280 0.19 +1.6 1,165,768 - MMJJ_RS06280 0.28 +2.9 1,165,834 - MMJJ_RS06280 0.35 -0.3 1,165,839 - MMJJ_RS06280 0.35 +2.3 1,165,843 + MMJJ_RS06280 0.36 +0.8 1,165,843 + MMJJ_RS06280 0.36 -1.2 1,165,845 + MMJJ_RS06280 0.36 -1.0 1,165,845 + MMJJ_RS06280 0.36 +1.7 1,165,868 + MMJJ_RS06280 0.38 +0.4 1,165,872 - MMJJ_RS06280 0.39 +3.5 1,165,874 - MMJJ_RS06280 0.39 +1.7 1,165,875 + MMJJ_RS06280 0.39 +0.4 1,165,926 + MMJJ_RS06280 0.45 +1.0 1,165,926 + MMJJ_RS06280 0.45 +0.9 1,165,927 - MMJJ_RS06280 0.45 +1.8 1,165,951 - MMJJ_RS06280 0.47 -1.7 1,165,952 + MMJJ_RS06280 0.47 +3.4 1,165,994 - MMJJ_RS06280 0.52 +0.8 1,165,995 + MMJJ_RS06280 0.52 +0.7 1,165,995 + MMJJ_RS06280 0.52 +0.8 1,166,004 + MMJJ_RS06280 0.53 +1.7 1,166,067 + MMJJ_RS06280 0.60 +0.7 1,166,089 + MMJJ_RS06280 0.62 -1.2
Or see this region's nucleotide sequence