Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06255 and MMJJ_RS09355 are separated by 15 nucleotides MMJJ_RS09355 and MMJJ_RS06260 are separated by 185 nucleotides MMJJ_RS06260 and MMJJ_RS06265 are separated by 8 nucleotides
MMJJ_RS06255: MMJJ_RS06255 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,160,643 to 1,162,265
_RS06255
MMJJ_RS09355: MMJJ_RS09355 - hypothetical protein, at 1,162,281 to 1,162,436
_RS09355
MMJJ_RS06260: MMJJ_RS06260 - 6-carboxyhexanoate--CoA ligase, at 1,162,622 to 1,163,356
_RS06260
MMJJ_RS06265: MMJJ_RS06265 - 8-amino-7-oxononanoate synthase, at 1,163,365 to 1,164,477
_RS06265
Position (kb)
1162
1163
1164 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1161.630 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.663 kb on + strand, within MMJJ_RS06255 at 1161.752 kb on - strand, within MMJJ_RS06255 at 1161.781 kb on + strand, within MMJJ_RS06255 at 1161.805 kb on - strand, within MMJJ_RS06255 at 1161.813 kb on + strand, within MMJJ_RS06255 at 1161.818 kb on - strand, within MMJJ_RS06255 at 1161.819 kb on + strand, within MMJJ_RS06255 at 1161.845 kb on - strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.929 kb on + strand, within MMJJ_RS06255 at 1162.193 kb on + strand at 1162.209 kb on - strand at 1162.242 kb on - strand at 1162.283 kb on + strand at 1162.294 kb on + strand at 1162.304 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.309 kb on + strand, within MMJJ_RS09355 at 1162.311 kb on - strand, within MMJJ_RS09355 at 1162.324 kb on - strand, within MMJJ_RS09355 at 1162.339 kb on + strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.432 kb on + strand at 1162.476 kb on - strand at 1162.478 kb on - strand at 1162.479 kb on + strand at 1162.480 kb on - strand at 1162.480 kb on - strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.482 kb on - strand at 1162.482 kb on - strand at 1162.483 kb on + strand at 1162.483 kb on + strand at 1162.499 kb on - strand at 1162.572 kb on + strand at 1162.591 kb on - strand at 1162.592 kb on + strand at 1162.617 kb on + strand at 1162.765 kb on - strand, within MMJJ_RS06260 at 1162.800 kb on - strand, within MMJJ_RS06260 at 1162.805 kb on - strand, within MMJJ_RS06260 at 1162.876 kb on - strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.960 kb on - strand, within MMJJ_RS06260 at 1163.080 kb on + strand, within MMJJ_RS06260 at 1163.182 kb on - strand, within MMJJ_RS06260 at 1163.201 kb on - strand, within MMJJ_RS06260 at 1163.213 kb on - strand, within MMJJ_RS06260 at 1163.214 kb on + strand, within MMJJ_RS06260 at 1163.225 kb on + strand, within MMJJ_RS06260 at 1163.239 kb on - strand, within MMJJ_RS06260 at 1163.240 kb on + strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.284 kb on + strand at 1163.335 kb on - strand at 1163.351 kb on - strand at 1163.352 kb on + strand at 1163.352 kb on + strand at 1163.354 kb on - strand at 1163.355 kb on + strand at 1163.365 kb on + strand at 1163.506 kb on + strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.513 kb on + strand, within MMJJ_RS06265 at 1163.531 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.635 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.670 kb on + strand, within MMJJ_RS06265 at 1163.683 kb on + strand, within MMJJ_RS06265 at 1163.714 kb on - strand, within MMJJ_RS06265 at 1163.730 kb on + strand, within MMJJ_RS06265 at 1163.742 kb on + strand, within MMJJ_RS06265 at 1163.782 kb on + strand, within MMJJ_RS06265 at 1163.798 kb on - strand, within MMJJ_RS06265 at 1163.799 kb on + strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.961 kb on + strand, within MMJJ_RS06265 at 1164.013 kb on + strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.066 kb on + strand, within MMJJ_RS06265 at 1164.092 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.160 kb on + strand, within MMJJ_RS06265 at 1164.274 kb on + strand, within MMJJ_RS06265
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,161,630 + MMJJ_RS06255 0.61 +1.5 1,161,656 + MMJJ_RS06255 0.62 -1.5 1,161,656 + MMJJ_RS06255 0.62 -0.5 1,161,663 + MMJJ_RS06255 0.63 -1.5 1,161,752 - MMJJ_RS06255 0.68 -0.1 1,161,781 + MMJJ_RS06255 0.70 -0.1 1,161,805 - MMJJ_RS06255 0.72 +0.5 1,161,813 + MMJJ_RS06255 0.72 -0.5 1,161,818 - MMJJ_RS06255 0.72 -0.9 1,161,819 + MMJJ_RS06255 0.72 -2.0 1,161,845 - MMJJ_RS06255 0.74 -1.1 1,161,850 + MMJJ_RS06255 0.74 -0.5 1,161,850 + MMJJ_RS06255 0.74 +1.5 1,161,929 + MMJJ_RS06255 0.79 -0.5 1,162,193 + -1.9 1,162,209 - -1.4 1,162,242 - -1.5 1,162,283 + -2.0 1,162,294 + +2.6 1,162,304 - MMJJ_RS09355 0.15 -1.7 1,162,308 - MMJJ_RS09355 0.17 -1.7 1,162,308 - MMJJ_RS09355 0.17 -2.4 1,162,309 + MMJJ_RS09355 0.18 +3.0 1,162,311 - MMJJ_RS09355 0.19 +1.8 1,162,324 - MMJJ_RS09355 0.28 +0.3 1,162,339 + MMJJ_RS09355 0.37 -1.2 1,162,367 - MMJJ_RS09355 0.55 -1.7 1,162,367 - MMJJ_RS09355 0.55 +2.8 1,162,432 + +0.5 1,162,476 - -1.4 1,162,478 - +2.4 1,162,479 + +3.4 1,162,480 - -3.6 1,162,480 - -1.2 1,162,481 + +3.9 1,162,481 + -0.4 1,162,481 + -1.7 1,162,481 + +0.3 1,162,482 - -1.1 1,162,482 - -0.4 1,162,483 + -1.9 1,162,483 + +0.4 1,162,499 - +0.7 1,162,572 + +1.2 1,162,591 - -1.5 1,162,592 + +1.2 1,162,617 + -0.5 1,162,765 - MMJJ_RS06260 0.19 -1.4 1,162,800 - MMJJ_RS06260 0.24 -2.3 1,162,805 - MMJJ_RS06260 0.25 -1.4 1,162,876 - MMJJ_RS06260 0.35 -3.7 1,162,877 + MMJJ_RS06260 0.35 -1.3 1,162,877 + MMJJ_RS06260 0.35 -0.1 1,162,960 - MMJJ_RS06260 0.46 -3.5 1,163,080 + MMJJ_RS06260 0.62 +1.4 1,163,182 - MMJJ_RS06260 0.76 -2.4 1,163,201 - MMJJ_RS06260 0.79 +0.6 1,163,213 - MMJJ_RS06260 0.80 -1.9 1,163,214 + MMJJ_RS06260 0.81 -0.7 1,163,225 + MMJJ_RS06260 0.82 +0.3 1,163,239 - MMJJ_RS06260 0.84 -1.5 1,163,240 + MMJJ_RS06260 0.84 -0.1 1,163,247 - MMJJ_RS06260 0.85 -2.6 1,163,247 - MMJJ_RS06260 0.85 +1.6 1,163,284 + +0.8 1,163,335 - -0.8 1,163,351 - -1.6 1,163,352 + -1.1 1,163,352 + +1.6 1,163,354 - -1.7 1,163,355 + -0.2 1,163,365 + +2.0 1,163,506 + MMJJ_RS06265 0.13 -1.7 1,163,512 - MMJJ_RS06265 0.13 -1.6 1,163,512 - MMJJ_RS06265 0.13 -1.1 1,163,512 - MMJJ_RS06265 0.13 -2.3 1,163,512 - MMJJ_RS06265 0.13 -1.4 1,163,512 - MMJJ_RS06265 0.13 +1.4 1,163,513 + MMJJ_RS06265 0.13 -2.1 1,163,531 + MMJJ_RS06265 0.15 -1.7 1,163,574 + MMJJ_RS06265 0.19 -2.6 1,163,574 + MMJJ_RS06265 0.19 -2.3 1,163,574 + MMJJ_RS06265 0.19 -0.2 1,163,635 - MMJJ_RS06265 0.24 -0.9 1,163,660 - MMJJ_RS06265 0.27 -0.1 1,163,660 - MMJJ_RS06265 0.27 +0.2 1,163,667 - MMJJ_RS06265 0.27 -0.0 1,163,667 - MMJJ_RS06265 0.27 +1.4 1,163,667 - MMJJ_RS06265 0.27 -1.1 1,163,669 - MMJJ_RS06265 0.27 +1.4 1,163,669 - MMJJ_RS06265 0.27 -1.7 1,163,669 - MMJJ_RS06265 0.27 -3.1 1,163,669 - MMJJ_RS06265 0.27 +0.4 1,163,670 + MMJJ_RS06265 0.27 +0.9 1,163,683 + MMJJ_RS06265 0.29 -2.3 1,163,714 - MMJJ_RS06265 0.31 -1.3 1,163,730 + MMJJ_RS06265 0.33 -1.1 1,163,742 + MMJJ_RS06265 0.34 -2.4 1,163,782 + MMJJ_RS06265 0.37 -2.0 1,163,798 - MMJJ_RS06265 0.39 +0.4 1,163,799 + MMJJ_RS06265 0.39 -0.6 1,163,843 - MMJJ_RS06265 0.43 -1.4 1,163,843 - MMJJ_RS06265 0.43 -1.7 1,163,843 - MMJJ_RS06265 0.43 -1.1 1,163,843 - MMJJ_RS06265 0.43 -1.2 1,163,844 + MMJJ_RS06265 0.43 +0.4 1,163,844 + MMJJ_RS06265 0.43 -2.4 1,163,844 + MMJJ_RS06265 0.43 -1.1 1,163,914 - MMJJ_RS06265 0.49 +0.1 1,163,914 - MMJJ_RS06265 0.49 -1.6 1,163,928 + MMJJ_RS06265 0.51 -2.0 1,163,928 + MMJJ_RS06265 0.51 +1.4 1,163,961 + MMJJ_RS06265 0.54 -0.6 1,164,013 + MMJJ_RS06265 0.58 +0.5 1,164,065 - MMJJ_RS06265 0.63 -0.6 1,164,065 - MMJJ_RS06265 0.63 -1.4 1,164,065 - MMJJ_RS06265 0.63 -0.4 1,164,065 - MMJJ_RS06265 0.63 +1.6 1,164,066 + MMJJ_RS06265 0.63 -1.7 1,164,092 + MMJJ_RS06265 0.65 -1.2 1,164,118 + MMJJ_RS06265 0.68 -0.5 1,164,118 + MMJJ_RS06265 0.68 -1.7 1,164,160 + MMJJ_RS06265 0.71 -1.1 1,164,274 + MMJJ_RS06265 0.82 -2.6
Or see this region's nucleotide sequence