Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06245 and MMJJ_RS06250 are separated by 128 nucleotides MMJJ_RS06250 and MMJJ_RS06255 are separated by 130 nucleotides MMJJ_RS06255 and MMJJ_RS09355 are separated by 15 nucleotides MMJJ_RS09355 and MMJJ_RS06260 are separated by 185 nucleotides
MMJJ_RS06245: MMJJ_RS06245 - 30S ribosomal protein S15, at 1,159,413 to 1,159,868
_RS06245
MMJJ_RS06250: MMJJ_RS06250 - nicotinamide-nucleotide adenylyltransferase, at 1,159,997 to 1,160,512
_RS06250
MMJJ_RS06255: MMJJ_RS06255 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,160,643 to 1,162,265
_RS06255
MMJJ_RS09355: MMJJ_RS09355 - hypothetical protein, at 1,162,281 to 1,162,436
_RS09355
MMJJ_RS06260: MMJJ_RS06260 - 6-carboxyhexanoate--CoA ligase, at 1,162,622 to 1,163,356
_RS06260
Position (kb)
1160
1161
1162
1163 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1159.857 kb on - strand at 1159.958 kb on - strand at 1159.958 kb on - strand at 1159.962 kb on - strand at 1159.976 kb on - strand at 1159.987 kb on - strand at 1159.988 kb on + strand at 1160.063 kb on - strand, within MMJJ_RS06250 at 1160.088 kb on + strand at 1160.152 kb on - strand, within MMJJ_RS06250 at 1160.182 kb on + strand, within MMJJ_RS06250 at 1160.198 kb on - strand, within MMJJ_RS06250 at 1160.236 kb on - strand at 1160.281 kb on - strand at 1160.281 kb on - strand at 1160.283 kb on - strand, within MMJJ_RS06250 at 1160.283 kb on - strand, within MMJJ_RS06250 at 1160.288 kb on - strand, within MMJJ_RS06250 at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.546 kb on + strand at 1160.551 kb on - strand at 1160.552 kb on + strand at 1160.556 kb on - strand at 1160.557 kb on + strand at 1160.580 kb on + strand at 1160.583 kb on + strand at 1160.586 kb on - strand at 1160.586 kb on - strand at 1160.587 kb on + strand at 1160.593 kb on + strand at 1160.593 kb on + strand at 1160.594 kb on - strand at 1160.595 kb on + strand at 1160.605 kb on - strand at 1160.606 kb on + strand at 1160.607 kb on - strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.609 kb on - strand at 1160.609 kb on - strand at 1160.609 kb on - strand at 1160.610 kb on + strand at 1160.610 kb on + strand at 1160.613 kb on + strand at 1160.620 kb on + strand at 1160.666 kb on + strand at 1160.689 kb on - strand at 1160.690 kb on + strand at 1160.973 kb on + strand, within MMJJ_RS06255 at 1160.998 kb on + strand, within MMJJ_RS06255 at 1161.036 kb on - strand, within MMJJ_RS06255 at 1161.037 kb on + strand, within MMJJ_RS06255 at 1161.052 kb on + strand, within MMJJ_RS06255 at 1161.112 kb on - strand, within MMJJ_RS06255 at 1161.366 kb on + strand at 1161.422 kb on - strand at 1161.465 kb on - strand, within MMJJ_RS06255 at 1161.466 kb on + strand at 1161.603 kb on - strand, within MMJJ_RS06255 at 1161.604 kb on + strand, within MMJJ_RS06255 at 1161.611 kb on + strand, within MMJJ_RS06255 at 1161.630 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.656 kb on + strand, within MMJJ_RS06255 at 1161.663 kb on + strand, within MMJJ_RS06255 at 1161.752 kb on - strand, within MMJJ_RS06255 at 1161.781 kb on + strand, within MMJJ_RS06255 at 1161.805 kb on - strand, within MMJJ_RS06255 at 1161.813 kb on + strand, within MMJJ_RS06255 at 1161.818 kb on - strand, within MMJJ_RS06255 at 1161.819 kb on + strand, within MMJJ_RS06255 at 1161.845 kb on - strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.850 kb on + strand, within MMJJ_RS06255 at 1161.929 kb on + strand, within MMJJ_RS06255 at 1162.193 kb on + strand at 1162.209 kb on - strand at 1162.242 kb on - strand at 1162.283 kb on + strand at 1162.294 kb on + strand at 1162.304 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.308 kb on - strand, within MMJJ_RS09355 at 1162.309 kb on + strand, within MMJJ_RS09355 at 1162.311 kb on - strand, within MMJJ_RS09355 at 1162.324 kb on - strand, within MMJJ_RS09355 at 1162.339 kb on + strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.432 kb on + strand at 1162.476 kb on - strand at 1162.478 kb on - strand at 1162.479 kb on + strand at 1162.480 kb on - strand at 1162.480 kb on - strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.482 kb on - strand at 1162.482 kb on - strand at 1162.483 kb on + strand at 1162.483 kb on + strand at 1162.499 kb on - strand at 1162.572 kb on + strand at 1162.591 kb on - strand at 1162.592 kb on + strand at 1162.617 kb on + strand at 1162.765 kb on - strand, within MMJJ_RS06260 at 1162.800 kb on - strand, within MMJJ_RS06260 at 1162.805 kb on - strand, within MMJJ_RS06260 at 1162.876 kb on - strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.960 kb on - strand, within MMJJ_RS06260 at 1163.080 kb on + strand, within MMJJ_RS06260 at 1163.182 kb on - strand, within MMJJ_RS06260 at 1163.201 kb on - strand, within MMJJ_RS06260 at 1163.213 kb on - strand, within MMJJ_RS06260 at 1163.214 kb on + strand, within MMJJ_RS06260 at 1163.225 kb on + strand, within MMJJ_RS06260 at 1163.239 kb on - strand, within MMJJ_RS06260 at 1163.240 kb on + strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,159,857 - +1.5 1,159,958 - +2.5 1,159,958 - -0.1 1,159,962 - +1.2 1,159,976 - -1.1 1,159,987 - -1.5 1,159,988 + -2.5 1,160,063 - MMJJ_RS06250 0.13 -0.9 1,160,088 + +0.5 1,160,152 - MMJJ_RS06250 0.30 +0.5 1,160,182 + MMJJ_RS06250 0.36 -0.7 1,160,198 - MMJJ_RS06250 0.39 +2.5 1,160,236 - +1.5 1,160,281 - +0.5 1,160,281 - +0.5 1,160,283 - MMJJ_RS06250 0.55 -0.1 1,160,283 - MMJJ_RS06250 0.55 +0.5 1,160,288 - MMJJ_RS06250 0.56 -0.1 1,160,545 - -0.1 1,160,545 - -1.4 1,160,545 - -1.6 1,160,545 - -0.0 1,160,545 - -1.9 1,160,546 + -0.5 1,160,551 - -4.1 1,160,552 + -0.1 1,160,556 - -1.8 1,160,557 + -1.5 1,160,580 + +0.5 1,160,583 + -1.5 1,160,586 - -1.5 1,160,586 - -1.0 1,160,587 + -3.1 1,160,593 + -1.1 1,160,593 + -1.5 1,160,594 - -0.1 1,160,595 + -0.9 1,160,605 - +0.5 1,160,606 + -1.9 1,160,607 - -1.9 1,160,608 + -1.5 1,160,608 + -0.4 1,160,608 + -1.5 1,160,608 + -2.4 1,160,608 + -2.5 1,160,609 - -1.7 1,160,609 - +0.9 1,160,609 - -0.1 1,160,610 + +1.5 1,160,610 + +0.0 1,160,613 + -2.5 1,160,620 + -0.1 1,160,666 + -1.5 1,160,689 - -0.4 1,160,690 + +1.5 1,160,973 + MMJJ_RS06255 0.20 -0.7 1,160,998 + MMJJ_RS06255 0.22 +0.0 1,161,036 - MMJJ_RS06255 0.24 -1.5 1,161,037 + MMJJ_RS06255 0.24 +1.2 1,161,052 + MMJJ_RS06255 0.25 -1.5 1,161,112 - MMJJ_RS06255 0.29 -1.4 1,161,366 + -0.1 1,161,422 - -0.1 1,161,465 - MMJJ_RS06255 0.51 -1.4 1,161,466 + +1.5 1,161,603 - MMJJ_RS06255 0.59 -1.5 1,161,604 + MMJJ_RS06255 0.59 +0.5 1,161,611 + MMJJ_RS06255 0.60 -0.5 1,161,630 + MMJJ_RS06255 0.61 +1.5 1,161,656 + MMJJ_RS06255 0.62 -1.5 1,161,656 + MMJJ_RS06255 0.62 -0.5 1,161,663 + MMJJ_RS06255 0.63 -1.5 1,161,752 - MMJJ_RS06255 0.68 -0.1 1,161,781 + MMJJ_RS06255 0.70 -0.1 1,161,805 - MMJJ_RS06255 0.72 +0.5 1,161,813 + MMJJ_RS06255 0.72 -0.5 1,161,818 - MMJJ_RS06255 0.72 -0.9 1,161,819 + MMJJ_RS06255 0.72 -2.0 1,161,845 - MMJJ_RS06255 0.74 -1.1 1,161,850 + MMJJ_RS06255 0.74 -0.5 1,161,850 + MMJJ_RS06255 0.74 +1.5 1,161,929 + MMJJ_RS06255 0.79 -0.5 1,162,193 + -1.9 1,162,209 - -1.4 1,162,242 - -1.5 1,162,283 + -2.0 1,162,294 + +2.6 1,162,304 - MMJJ_RS09355 0.15 -1.7 1,162,308 - MMJJ_RS09355 0.17 -1.7 1,162,308 - MMJJ_RS09355 0.17 -2.4 1,162,309 + MMJJ_RS09355 0.18 +3.0 1,162,311 - MMJJ_RS09355 0.19 +1.8 1,162,324 - MMJJ_RS09355 0.28 +0.3 1,162,339 + MMJJ_RS09355 0.37 -1.2 1,162,367 - MMJJ_RS09355 0.55 -1.7 1,162,367 - MMJJ_RS09355 0.55 +2.8 1,162,432 + +0.5 1,162,476 - -1.4 1,162,478 - +2.4 1,162,479 + +3.4 1,162,480 - -3.6 1,162,480 - -1.2 1,162,481 + +3.9 1,162,481 + -0.4 1,162,481 + -1.7 1,162,481 + +0.3 1,162,482 - -1.1 1,162,482 - -0.4 1,162,483 + -1.9 1,162,483 + +0.4 1,162,499 - +0.7 1,162,572 + +1.2 1,162,591 - -1.5 1,162,592 + +1.2 1,162,617 + -0.5 1,162,765 - MMJJ_RS06260 0.19 -1.4 1,162,800 - MMJJ_RS06260 0.24 -2.3 1,162,805 - MMJJ_RS06260 0.25 -1.4 1,162,876 - MMJJ_RS06260 0.35 -3.7 1,162,877 + MMJJ_RS06260 0.35 -1.3 1,162,877 + MMJJ_RS06260 0.35 -0.1 1,162,960 - MMJJ_RS06260 0.46 -3.5 1,163,080 + MMJJ_RS06260 0.62 +1.4 1,163,182 - MMJJ_RS06260 0.76 -2.4 1,163,201 - MMJJ_RS06260 0.79 +0.6 1,163,213 - MMJJ_RS06260 0.80 -1.9 1,163,214 + MMJJ_RS06260 0.81 -0.7 1,163,225 + MMJJ_RS06260 0.82 +0.3 1,163,239 - MMJJ_RS06260 0.84 -1.5 1,163,240 + MMJJ_RS06260 0.84 -0.1 1,163,247 - MMJJ_RS06260 0.85 -2.6 1,163,247 - MMJJ_RS06260 0.85 +1.6
Or see this region's nucleotide sequence