Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05805 and MMJJ_RS05810 are separated by 29 nucleotides MMJJ_RS05810 and MMJJ_RS05815 overlap by 4 nucleotides MMJJ_RS05815 and MMJJ_RS05820 overlap by 11 nucleotides MMJJ_RS05820 and MMJJ_RS05825 are separated by 85 nucleotides
MMJJ_RS05805: MMJJ_RS05805 - aconitase X catalytic domain-containing protein, at 1,081,885 to 1,083,090
_RS05805
MMJJ_RS05810: MMJJ_RS05810 - precorrin-4 C(11)-methyltransferase, at 1,083,120 to 1,083,884
_RS05810
MMJJ_RS05815: MMJJ_RS05815 - hypothetical protein, at 1,083,881 to 1,084,078
_RS05815
MMJJ_RS05820: MMJJ_RS05820 - DUF2304 domain-containing protein, at 1,084,068 to 1,084,427
_RS05820
MMJJ_RS05825: MMJJ_RS05825 - aspartate carbamoyltransferase, at 1,084,513 to 1,085,421
_RS05825
Position (kb)
1084
1085 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1083.207 kb on + strand, within MMJJ_RS05810 at 1083.211 kb on - strand, within MMJJ_RS05810 at 1083.212 kb on + strand, within MMJJ_RS05810 at 1083.212 kb on + strand at 1083.212 kb on + strand, within MMJJ_RS05810 at 1083.332 kb on - strand, within MMJJ_RS05810 at 1083.344 kb on + strand, within MMJJ_RS05810 at 1083.509 kb on + strand, within MMJJ_RS05810 at 1083.520 kb on - strand, within MMJJ_RS05810 at 1083.523 kb on - strand, within MMJJ_RS05810 at 1083.523 kb on - strand at 1083.597 kb on + strand, within MMJJ_RS05810 at 1083.641 kb on - strand, within MMJJ_RS05810 at 1083.642 kb on + strand, within MMJJ_RS05810 at 1083.642 kb on + strand, within MMJJ_RS05810 at 1083.649 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.692 kb on + strand, within MMJJ_RS05810 at 1083.693 kb on - strand, within MMJJ_RS05810 at 1083.737 kb on + strand, within MMJJ_RS05810 at 1083.745 kb on - strand, within MMJJ_RS05810 at 1083.788 kb on - strand, within MMJJ_RS05810 at 1083.789 kb on + strand, within MMJJ_RS05810 at 1083.789 kb on + strand at 1083.790 kb on - strand, within MMJJ_RS05810 at 1083.790 kb on - strand at 1083.822 kb on - strand at 1083.823 kb on + strand at 1083.891 kb on + strand at 1083.899 kb on - strand at 1083.912 kb on + strand, within MMJJ_RS05815 at 1083.953 kb on + strand, within MMJJ_RS05815 at 1083.953 kb on + strand, within MMJJ_RS05815 at 1083.956 kb on - strand, within MMJJ_RS05815 at 1083.956 kb on - strand, within MMJJ_RS05815 at 1083.980 kb on - strand, within MMJJ_RS05815 at 1084.021 kb on - strand, within MMJJ_RS05815 at 1084.021 kb on - strand, within MMJJ_RS05815 at 1084.022 kb on + strand, within MMJJ_RS05815 at 1084.061 kb on - strand at 1084.062 kb on + strand at 1084.105 kb on - strand, within MMJJ_RS05820 at 1084.115 kb on - strand, within MMJJ_RS05820 at 1084.204 kb on + strand, within MMJJ_RS05820 at 1084.204 kb on + strand, within MMJJ_RS05820 at 1084.205 kb on - strand, within MMJJ_RS05820 at 1084.299 kb on - strand, within MMJJ_RS05820 at 1084.325 kb on - strand, within MMJJ_RS05820 at 1084.325 kb on - strand, within MMJJ_RS05820 at 1084.325 kb on - strand, within MMJJ_RS05820 at 1084.326 kb on + strand, within MMJJ_RS05820 at 1084.335 kb on + strand, within MMJJ_RS05820 at 1084.335 kb on + strand, within MMJJ_RS05820 at 1084.346 kb on - strand, within MMJJ_RS05820 at 1084.389 kb on - strand, within MMJJ_RS05820 at 1084.389 kb on - strand, within MMJJ_RS05820 at 1084.389 kb on - strand, within MMJJ_RS05820 at 1084.390 kb on + strand, within MMJJ_RS05820 at 1084.446 kb on - strand at 1084.446 kb on - strand at 1084.446 kb on - strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.447 kb on + strand at 1084.448 kb on - strand at 1084.448 kb on - strand at 1084.449 kb on + strand at 1084.450 kb on - strand at 1084.450 kb on - strand at 1084.450 kb on - strand at 1084.450 kb on - strand at 1084.451 kb on + strand at 1084.451 kb on + strand at 1084.451 kb on + strand at 1084.451 kb on + strand at 1084.455 kb on - strand at 1084.456 kb on + strand at 1084.456 kb on + strand at 1084.469 kb on + strand at 1084.470 kb on - strand at 1084.472 kb on - strand at 1084.473 kb on + strand at 1084.473 kb on + strand at 1084.473 kb on + strand at 1084.474 kb on - strand at 1084.484 kb on + strand at 1084.484 kb on + strand at 1084.485 kb on - strand at 1085.216 kb on + strand, within MMJJ_RS05825 at 1085.225 kb on - strand, within MMJJ_RS05825 at 1085.235 kb on + strand, within MMJJ_RS05825 at 1085.391 kb on + strand at 1085.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,083,207 + MMJJ_RS05810 0.11 +0.1 1,083,211 - MMJJ_RS05810 0.12 -0.9 1,083,212 + MMJJ_RS05810 0.12 -0.6 1,083,212 + +0.4 1,083,212 + MMJJ_RS05810 0.12 -1.4 1,083,332 - MMJJ_RS05810 0.28 +0.8 1,083,344 + MMJJ_RS05810 0.29 -0.9 1,083,509 + MMJJ_RS05810 0.51 -1.4 1,083,520 - MMJJ_RS05810 0.52 -0.5 1,083,523 - MMJJ_RS05810 0.53 -0.6 1,083,523 - +0.4 1,083,597 + MMJJ_RS05810 0.62 +1.1 1,083,641 - MMJJ_RS05810 0.68 -0.2 1,083,642 + MMJJ_RS05810 0.68 +1.4 1,083,642 + MMJJ_RS05810 0.68 -0.6 1,083,649 - MMJJ_RS05810 0.69 -1.4 1,083,691 - MMJJ_RS05810 0.75 -0.4 1,083,691 - MMJJ_RS05810 0.75 -1.2 1,083,691 - MMJJ_RS05810 0.75 +4.9 1,083,692 + MMJJ_RS05810 0.75 -0.2 1,083,693 - MMJJ_RS05810 0.75 +0.4 1,083,737 + MMJJ_RS05810 0.81 +1.3 1,083,745 - MMJJ_RS05810 0.82 -0.2 1,083,788 - MMJJ_RS05810 0.87 -0.3 1,083,789 + MMJJ_RS05810 0.87 -0.9 1,083,789 + +1.4 1,083,790 - MMJJ_RS05810 0.88 -0.4 1,083,790 - +1.4 1,083,822 - +0.4 1,083,823 + -0.2 1,083,891 + -0.9 1,083,899 - -4.3 1,083,912 + MMJJ_RS05815 0.16 -0.7 1,083,953 + MMJJ_RS05815 0.36 -0.2 1,083,953 + MMJJ_RS05815 0.36 -0.8 1,083,956 - MMJJ_RS05815 0.38 -0.4 1,083,956 - MMJJ_RS05815 0.38 +1.7 1,083,980 - MMJJ_RS05815 0.50 +1.8 1,084,021 - MMJJ_RS05815 0.71 +2.0 1,084,021 - MMJJ_RS05815 0.71 +1.9 1,084,022 + MMJJ_RS05815 0.71 +2.1 1,084,061 - +0.4 1,084,062 + +1.4 1,084,105 - MMJJ_RS05820 0.10 -2.9 1,084,115 - MMJJ_RS05820 0.13 -0.6 1,084,204 + MMJJ_RS05820 0.38 +3.9 1,084,204 + MMJJ_RS05820 0.38 +5.4 1,084,205 - MMJJ_RS05820 0.38 +3.2 1,084,299 - MMJJ_RS05820 0.64 -1.4 1,084,325 - MMJJ_RS05820 0.71 +2.9 1,084,325 - MMJJ_RS05820 0.71 +3.5 1,084,325 - MMJJ_RS05820 0.71 +0.4 1,084,326 + MMJJ_RS05820 0.72 +3.0 1,084,335 + MMJJ_RS05820 0.74 +2.5 1,084,335 + MMJJ_RS05820 0.74 -0.9 1,084,346 - MMJJ_RS05820 0.77 -3.2 1,084,389 - MMJJ_RS05820 0.89 -0.1 1,084,389 - MMJJ_RS05820 0.89 +3.6 1,084,389 - MMJJ_RS05820 0.89 -1.8 1,084,390 + MMJJ_RS05820 0.89 +3.0 1,084,446 - -1.1 1,084,446 - +1.5 1,084,446 - -3.5 1,084,447 + -2.1 1,084,447 + +1.1 1,084,447 + -2.2 1,084,447 + -1.9 1,084,447 + -2.4 1,084,447 + +3.3 1,084,447 + -2.5 1,084,447 + +0.7 1,084,447 + +1.1 1,084,447 + -1.8 1,084,447 + +3.7 1,084,447 + +1.8 1,084,447 + +3.5 1,084,448 - -3.2 1,084,448 - -1.9 1,084,449 + +1.0 1,084,450 - -1.7 1,084,450 - +5.2 1,084,450 - -1.8 1,084,450 - +1.8 1,084,451 + +2.9 1,084,451 + -2.2 1,084,451 + +0.1 1,084,451 + -0.8 1,084,455 - -2.1 1,084,456 + -1.1 1,084,456 + -1.2 1,084,469 + +0.3 1,084,470 - +0.4 1,084,472 - -0.8 1,084,473 + +1.4 1,084,473 + +1.0 1,084,473 + +0.9 1,084,474 - +1.4 1,084,484 + +0.3 1,084,484 + -1.0 1,084,485 - -0.4 1,085,216 + MMJJ_RS05825 0.77 -0.2 1,085,225 - MMJJ_RS05825 0.78 -0.6 1,085,235 + MMJJ_RS05825 0.79 +0.4 1,085,391 + +2.6 1,085,423 - -2.7
Or see this region's nucleotide sequence