Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05565 and MMJJ_RS05570 are separated by 8 nucleotides MMJJ_RS05570 and MMJJ_RS05575 are separated by 5 nucleotides MMJJ_RS05575 and MMJJ_RS05580 are separated by 9 nucleotides MMJJ_RS05580 and MMJJ_RS05585 overlap by 4 nucleotides
MMJJ_RS05565: MMJJ_RS05565 - translation initiation factor IF-2 subunit alpha, at 1,039,616 to 1,040,413
_RS05565
MMJJ_RS05570: MMJJ_RS05570 - RNA-protein complex protein Nop10, at 1,040,422 to 1,040,577
_RS05570
MMJJ_RS05575: MMJJ_RS05575 - 2-amino-5-formylamino-6-ribosylaminopyrimidin- 4(3H)-one 5'-monophosphate deformylase, at 1,040,583 to 1,041,248
_RS05575
MMJJ_RS05580: MMJJ_RS05580 - methanogenesis marker 9 domain-containing protein, at 1,041,258 to 1,041,713
_RS05580
MMJJ_RS05585: MMJJ_RS05585 - hypothetical protein, at 1,041,710 to 1,042,606
_RS05585
Position (kb)
1040
1041
1042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1039.594 kb on + strand at 1039.648 kb on - strand at 1039.765 kb on - strand, within MMJJ_RS05565 at 1039.767 kb on - strand, within MMJJ_RS05565 at 1039.819 kb on + strand, within MMJJ_RS05565 at 1040.100 kb on - strand, within MMJJ_RS05565 at 1040.456 kb on + strand, within MMJJ_RS05570 at 1040.613 kb on + strand at 1040.719 kb on + strand, within MMJJ_RS05575 at 1040.794 kb on + strand, within MMJJ_RS05575 at 1040.831 kb on + strand, within MMJJ_RS05575 at 1040.834 kb on - strand, within MMJJ_RS05575 at 1040.834 kb on - strand, within MMJJ_RS05575 at 1040.839 kb on + strand, within MMJJ_RS05575 at 1040.895 kb on + strand, within MMJJ_RS05575 at 1041.076 kb on + strand, within MMJJ_RS05575 at 1041.100 kb on - strand, within MMJJ_RS05575 at 1041.177 kb on + strand, within MMJJ_RS05575 at 1041.246 kb on - strand at 1041.247 kb on + strand at 1041.256 kb on + strand at 1041.256 kb on + strand at 1041.257 kb on - strand at 1041.257 kb on - strand at 1041.258 kb on + strand at 1041.332 kb on + strand, within MMJJ_RS05580 at 1041.332 kb on + strand, within MMJJ_RS05580 at 1041.337 kb on + strand, within MMJJ_RS05580 at 1041.346 kb on - strand, within MMJJ_RS05580 at 1041.351 kb on + strand, within MMJJ_RS05580 at 1041.397 kb on - strand, within MMJJ_RS05580 at 1041.416 kb on - strand, within MMJJ_RS05580 at 1041.417 kb on + strand, within MMJJ_RS05580 at 1041.417 kb on + strand, within MMJJ_RS05580 at 1041.428 kb on + strand, within MMJJ_RS05580 at 1041.443 kb on - strand, within MMJJ_RS05580 at 1041.473 kb on - strand, within MMJJ_RS05580 at 1041.474 kb on + strand, within MMJJ_RS05580 at 1041.498 kb on + strand, within MMJJ_RS05580 at 1041.540 kb on - strand, within MMJJ_RS05580 at 1041.540 kb on - strand, within MMJJ_RS05580 at 1041.540 kb on - strand, within MMJJ_RS05580 at 1041.540 kb on - strand at 1041.541 kb on + strand, within MMJJ_RS05580 at 1041.712 kb on + strand at 1041.716 kb on + strand at 1041.716 kb on + strand at 1041.748 kb on - strand at 1041.748 kb on - strand at 1041.799 kb on - strand at 1041.804 kb on + strand, within MMJJ_RS05585 at 1041.809 kb on + strand, within MMJJ_RS05585 at 1041.838 kb on - strand, within MMJJ_RS05585 at 1041.838 kb on - strand, within MMJJ_RS05585 at 1041.850 kb on + strand, within MMJJ_RS05585 at 1041.944 kb on - strand, within MMJJ_RS05585 at 1041.944 kb on - strand, within MMJJ_RS05585 at 1041.945 kb on + strand, within MMJJ_RS05585 at 1041.945 kb on + strand, within MMJJ_RS05585 at 1041.945 kb on + strand, within MMJJ_RS05585 at 1041.945 kb on + strand, within MMJJ_RS05585 at 1041.945 kb on + strand, within MMJJ_RS05585 at 1041.946 kb on - strand, within MMJJ_RS05585 at 1041.946 kb on - strand, within MMJJ_RS05585 at 1041.947 kb on + strand, within MMJJ_RS05585 at 1041.949 kb on - strand, within MMJJ_RS05585 at 1041.950 kb on + strand, within MMJJ_RS05585 at 1041.950 kb on + strand, within MMJJ_RS05585 at 1041.951 kb on - strand, within MMJJ_RS05585 at 1041.951 kb on - strand, within MMJJ_RS05585 at 1041.952 kb on + strand, within MMJJ_RS05585 at 1041.952 kb on + strand, within MMJJ_RS05585 at 1041.952 kb on + strand, within MMJJ_RS05585 at 1041.952 kb on + strand, within MMJJ_RS05585 at 1041.954 kb on + strand, within MMJJ_RS05585 at 1042.223 kb on - strand, within MMJJ_RS05585
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,039,594 + -0.9 1,039,648 - -1.9 1,039,765 - MMJJ_RS05565 0.19 -1.4 1,039,767 - MMJJ_RS05565 0.19 -0.6 1,039,819 + MMJJ_RS05565 0.25 +0.4 1,040,100 - MMJJ_RS05565 0.61 -1.2 1,040,456 + MMJJ_RS05570 0.22 -1.2 1,040,613 + -2.9 1,040,719 + MMJJ_RS05575 0.20 -1.2 1,040,794 + MMJJ_RS05575 0.32 -1.6 1,040,831 + MMJJ_RS05575 0.37 -0.6 1,040,834 - MMJJ_RS05575 0.38 +1.4 1,040,834 - MMJJ_RS05575 0.38 -1.9 1,040,839 + MMJJ_RS05575 0.38 -0.6 1,040,895 + MMJJ_RS05575 0.47 -0.6 1,041,076 + MMJJ_RS05575 0.74 -1.7 1,041,100 - MMJJ_RS05575 0.78 -2.3 1,041,177 + MMJJ_RS05575 0.89 -1.7 1,041,246 - -1.2 1,041,247 + -1.5 1,041,256 + -2.7 1,041,256 + +3.3 1,041,257 - +1.5 1,041,257 - +3.2 1,041,258 + -1.4 1,041,332 + MMJJ_RS05580 0.16 +4.2 1,041,332 + MMJJ_RS05580 0.16 +2.4 1,041,337 + MMJJ_RS05580 0.17 +0.1 1,041,346 - MMJJ_RS05580 0.19 -0.7 1,041,351 + MMJJ_RS05580 0.20 -2.0 1,041,397 - MMJJ_RS05580 0.30 -1.4 1,041,416 - MMJJ_RS05580 0.35 -2.3 1,041,417 + MMJJ_RS05580 0.35 +2.0 1,041,417 + MMJJ_RS05580 0.35 +2.3 1,041,428 + MMJJ_RS05580 0.37 -1.1 1,041,443 - MMJJ_RS05580 0.41 +0.4 1,041,473 - MMJJ_RS05580 0.47 +0.1 1,041,474 + MMJJ_RS05580 0.47 +0.1 1,041,498 + MMJJ_RS05580 0.53 -1.9 1,041,540 - MMJJ_RS05580 0.62 -2.8 1,041,540 - MMJJ_RS05580 0.62 +1.5 1,041,540 - MMJJ_RS05580 0.62 -2.6 1,041,540 - +1.4 1,041,541 + MMJJ_RS05580 0.62 -0.9 1,041,712 + -1.5 1,041,716 + -1.6 1,041,716 + -2.1 1,041,748 - +0.4 1,041,748 - -0.6 1,041,799 - -0.9 1,041,804 + MMJJ_RS05585 0.10 +0.6 1,041,809 + MMJJ_RS05585 0.11 -0.9 1,041,838 - MMJJ_RS05585 0.14 -2.8 1,041,838 - MMJJ_RS05585 0.14 -0.4 1,041,850 + MMJJ_RS05585 0.16 -2.8 1,041,944 - MMJJ_RS05585 0.26 +2.2 1,041,944 - MMJJ_RS05585 0.26 -1.5 1,041,945 + MMJJ_RS05585 0.26 -2.8 1,041,945 + MMJJ_RS05585 0.26 +1.9 1,041,945 + MMJJ_RS05585 0.26 -0.7 1,041,945 + MMJJ_RS05585 0.26 +1.3 1,041,945 + MMJJ_RS05585 0.26 -0.4 1,041,946 - MMJJ_RS05585 0.26 -3.1 1,041,946 - MMJJ_RS05585 0.26 -1.8 1,041,947 + MMJJ_RS05585 0.26 -0.2 1,041,949 - MMJJ_RS05585 0.27 +0.7 1,041,950 + MMJJ_RS05585 0.27 -1.8 1,041,950 + MMJJ_RS05585 0.27 -0.6 1,041,951 - MMJJ_RS05585 0.27 +0.6 1,041,951 - MMJJ_RS05585 0.27 -3.1 1,041,952 + MMJJ_RS05585 0.27 +3.7 1,041,952 + MMJJ_RS05585 0.27 -1.8 1,041,952 + MMJJ_RS05585 0.27 -2.7 1,041,952 + MMJJ_RS05585 0.27 -0.5 1,041,954 + MMJJ_RS05585 0.27 -0.7 1,042,223 - MMJJ_RS05585 0.57 +3.3
Or see this region's nucleotide sequence