Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05545 and MMJJ_RS05550 are separated by 33 nucleotides MMJJ_RS05550 and MMJJ_RS05555 overlap by 44 nucleotides MMJJ_RS05555 and MMJJ_RS05560 are separated by 22 nucleotides MMJJ_RS05560 and MMJJ_RS05565 are separated by 113 nucleotides MMJJ_RS05565 and MMJJ_RS05570 are separated by 8 nucleotides
MMJJ_RS05545: MMJJ_RS05545 - DNA polymerase sliding clamp, at 1,037,683 to 1,038,435
_RS05545
MMJJ_RS05550: MMJJ_RS05550 - hypothetical protein, at 1,038,469 to 1,039,056
_RS05550
MMJJ_RS05555: MMJJ_RS05555 - 50S ribosomal protein L44e, at 1,039,013 to 1,039,294
_RS05555
MMJJ_RS05560: MMJJ_RS05560 - 30S ribosomal protein S27e, at 1,039,317 to 1,039,502
_RS05560
MMJJ_RS05565: MMJJ_RS05565 - translation initiation factor IF-2 subunit alpha, at 1,039,616 to 1,040,413
_RS05565
MMJJ_RS05570: MMJJ_RS05570 - RNA-protein complex protein Nop10, at 1,040,422 to 1,040,577
_RS05570
Position (kb)
1039
1040 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1038.473 kb on + strand at 1038.482 kb on - strand at 1039.292 kb on - strand at 1039.292 kb on - strand at 1039.293 kb on + strand at 1039.325 kb on + strand at 1039.337 kb on - strand, within MMJJ_RS05560 at 1039.338 kb on + strand, within MMJJ_RS05560 at 1039.388 kb on - strand, within MMJJ_RS05560 at 1039.388 kb on - strand, within MMJJ_RS05560 at 1039.389 kb on + strand, within MMJJ_RS05560 at 1039.416 kb on + strand, within MMJJ_RS05560 at 1039.487 kb on + strand at 1039.492 kb on - strand at 1039.492 kb on - strand at 1039.493 kb on + strand at 1039.493 kb on + strand at 1039.495 kb on - strand at 1039.496 kb on + strand at 1039.500 kb on - strand at 1039.511 kb on + strand at 1039.520 kb on - strand at 1039.520 kb on - strand at 1039.547 kb on - strand at 1039.547 kb on - strand at 1039.550 kb on + strand at 1039.557 kb on - strand at 1039.558 kb on + strand at 1039.571 kb on - strand at 1039.572 kb on + strand at 1039.594 kb on + strand at 1039.648 kb on - strand at 1039.765 kb on - strand, within MMJJ_RS05565 at 1039.767 kb on - strand, within MMJJ_RS05565 at 1039.819 kb on + strand, within MMJJ_RS05565 at 1040.100 kb on - strand, within MMJJ_RS05565 at 1040.456 kb on + strand, within MMJJ_RS05570
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,038,473 + +0.4 1,038,482 - -1.6 1,039,292 - -0.6 1,039,292 - -0.2 1,039,293 + +3.2 1,039,325 + +2.5 1,039,337 - MMJJ_RS05560 0.11 -1.5 1,039,338 + MMJJ_RS05560 0.11 +0.1 1,039,388 - MMJJ_RS05560 0.38 -0.5 1,039,388 - MMJJ_RS05560 0.38 -2.0 1,039,389 + MMJJ_RS05560 0.39 -2.5 1,039,416 + MMJJ_RS05560 0.53 -1.8 1,039,487 + -1.3 1,039,492 - -0.7 1,039,492 - -2.6 1,039,493 + +1.8 1,039,493 + -2.1 1,039,495 - -2.7 1,039,496 + +1.4 1,039,500 - +3.5 1,039,511 + -1.3 1,039,520 - -3.6 1,039,520 - -1.0 1,039,547 - -1.3 1,039,547 - -0.8 1,039,550 + +0.2 1,039,557 - +0.8 1,039,558 + -0.3 1,039,571 - +0.4 1,039,572 + -0.9 1,039,594 + -0.9 1,039,648 - -1.9 1,039,765 - MMJJ_RS05565 0.19 -1.4 1,039,767 - MMJJ_RS05565 0.19 -0.6 1,039,819 + MMJJ_RS05565 0.25 +0.4 1,040,100 - MMJJ_RS05565 0.61 -1.2 1,040,456 + MMJJ_RS05570 0.22 -1.2
Or see this region's nucleotide sequence