Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05520 and MMJJ_RS05525 are separated by 10 nucleotides MMJJ_RS05525 and MMJJ_RS05530 are separated by 14 nucleotides MMJJ_RS05530 and MMJJ_RS05535 are separated by 171 nucleotides
MMJJ_RS05520: MMJJ_RS05520 - H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein, at 1,033,604 to 1,034,629
_RS05520
MMJJ_RS05525: MMJJ_RS05525 - GTP-binding protein, at 1,034,640 to 1,035,113
_RS05525
MMJJ_RS05530: MMJJ_RS05530 - roadblock/LC7 domain-containing protein, at 1,035,128 to 1,035,472
_RS05530
MMJJ_RS05535: MMJJ_RS05535 - hypothetical protein, at 1,035,644 to 1,036,588
_RS05535
Position (kb)
1034
1035
1036 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1033.673 kb on - strand at 1033.673 kb on - strand at 1033.674 kb on + strand at 1033.741 kb on - strand, within MMJJ_RS05520 at 1033.742 kb on + strand, within MMJJ_RS05520 at 1033.742 kb on + strand, within MMJJ_RS05520 at 1033.746 kb on - strand, within MMJJ_RS05520 at 1033.811 kb on + strand, within MMJJ_RS05520 at 1033.816 kb on + strand, within MMJJ_RS05520 at 1033.816 kb on + strand, within MMJJ_RS05520 at 1033.818 kb on - strand, within MMJJ_RS05520 at 1033.901 kb on + strand, within MMJJ_RS05520 at 1033.906 kb on - strand, within MMJJ_RS05520 at 1033.906 kb on - strand, within MMJJ_RS05520 at 1033.907 kb on + strand, within MMJJ_RS05520 at 1033.907 kb on + strand, within MMJJ_RS05520 at 1033.912 kb on + strand, within MMJJ_RS05520 at 1033.945 kb on - strand, within MMJJ_RS05520 at 1033.945 kb on - strand, within MMJJ_RS05520 at 1033.946 kb on + strand, within MMJJ_RS05520 at 1033.946 kb on + strand, within MMJJ_RS05520 at 1033.946 kb on + strand, within MMJJ_RS05520 at 1033.946 kb on + strand, within MMJJ_RS05520 at 1033.951 kb on - strand, within MMJJ_RS05520 at 1033.952 kb on + strand, within MMJJ_RS05520 at 1033.972 kb on + strand, within MMJJ_RS05520 at 1033.974 kb on + strand, within MMJJ_RS05520 at 1033.995 kb on - strand, within MMJJ_RS05520 at 1034.002 kb on - strand, within MMJJ_RS05520 at 1034.002 kb on - strand, within MMJJ_RS05520 at 1034.014 kb on - strand, within MMJJ_RS05520 at 1034.020 kb on - strand, within MMJJ_RS05520 at 1034.023 kb on - strand, within MMJJ_RS05520 at 1034.024 kb on + strand, within MMJJ_RS05520 at 1034.024 kb on + strand, within MMJJ_RS05520 at 1034.072 kb on - strand, within MMJJ_RS05520 at 1034.072 kb on - strand, within MMJJ_RS05520 at 1034.073 kb on + strand, within MMJJ_RS05520 at 1034.073 kb on + strand, within MMJJ_RS05520 at 1034.073 kb on + strand, within MMJJ_RS05520 at 1034.109 kb on + strand, within MMJJ_RS05520 at 1034.109 kb on + strand, within MMJJ_RS05520 at 1034.109 kb on + strand, within MMJJ_RS05520 at 1034.109 kb on + strand, within MMJJ_RS05520 at 1034.191 kb on + strand, within MMJJ_RS05520 at 1034.278 kb on + strand, within MMJJ_RS05520 at 1034.362 kb on + strand, within MMJJ_RS05520 at 1034.373 kb on - strand, within MMJJ_RS05520 at 1034.476 kb on + strand, within MMJJ_RS05520 at 1034.487 kb on - strand, within MMJJ_RS05520 at 1034.487 kb on - strand, within MMJJ_RS05520 at 1034.597 kb on + strand at 1034.600 kb on + strand at 1034.600 kb on + strand at 1034.608 kb on - strand at 1034.608 kb on - strand at 1034.612 kb on + strand at 1034.644 kb on - strand at 1034.699 kb on + strand, within MMJJ_RS05525 at 1034.710 kb on - strand, within MMJJ_RS05525 at 1034.745 kb on + strand, within MMJJ_RS05525 at 1034.763 kb on - strand, within MMJJ_RS05525 at 1034.763 kb on - strand, within MMJJ_RS05525 at 1034.764 kb on + strand, within MMJJ_RS05525 at 1034.768 kb on - strand, within MMJJ_RS05525 at 1034.866 kb on + strand, within MMJJ_RS05525 at 1034.876 kb on + strand, within MMJJ_RS05525 at 1034.918 kb on - strand, within MMJJ_RS05525 at 1034.919 kb on + strand, within MMJJ_RS05525 at 1034.919 kb on + strand, within MMJJ_RS05525 at 1034.937 kb on + strand, within MMJJ_RS05525 at 1034.937 kb on + strand, within MMJJ_RS05525 at 1034.939 kb on - strand, within MMJJ_RS05525 at 1034.939 kb on - strand, within MMJJ_RS05525 at 1034.939 kb on - strand, within MMJJ_RS05525 at 1034.940 kb on + strand, within MMJJ_RS05525 at 1034.940 kb on + strand, within MMJJ_RS05525 at 1034.964 kb on + strand, within MMJJ_RS05525 at 1035.011 kb on + strand, within MMJJ_RS05525 at 1035.015 kb on + strand, within MMJJ_RS05525 at 1035.015 kb on + strand, within MMJJ_RS05525 at 1035.029 kb on - strand, within MMJJ_RS05525 at 1035.029 kb on - strand, within MMJJ_RS05525 at 1035.031 kb on - strand, within MMJJ_RS05525 at 1035.045 kb on + strand, within MMJJ_RS05525 at 1035.079 kb on - strand at 1035.088 kb on - strand at 1035.089 kb on + strand at 1035.089 kb on + strand at 1035.111 kb on - strand at 1035.123 kb on - strand at 1035.123 kb on - strand at 1035.123 kb on - strand at 1035.124 kb on + strand at 1035.124 kb on + strand at 1035.124 kb on + strand at 1035.124 kb on + strand at 1035.124 kb on + strand at 1035.124 kb on + strand at 1035.127 kb on - strand at 1035.128 kb on + strand at 1035.128 kb on + strand at 1035.128 kb on + strand at 1035.172 kb on + strand, within MMJJ_RS05530 at 1035.190 kb on + strand, within MMJJ_RS05530 at 1035.190 kb on + strand, within MMJJ_RS05530 at 1035.390 kb on - strand, within MMJJ_RS05530 at 1035.391 kb on + strand, within MMJJ_RS05530 at 1035.391 kb on + strand, within MMJJ_RS05530 at 1035.430 kb on + strand, within MMJJ_RS05530 at 1035.506 kb on - strand at 1035.520 kb on + strand at 1035.538 kb on + strand at 1035.548 kb on + strand at 1035.550 kb on - strand at 1035.571 kb on - strand at 1035.618 kb on - strand at 1035.618 kb on - strand at 1035.619 kb on + strand at 1035.619 kb on + strand at 1035.619 kb on + strand at 1035.624 kb on - strand at 1035.625 kb on + strand at 1035.625 kb on + strand at 1035.625 kb on + strand at 1035.625 kb on + strand at 1035.656 kb on - strand at 1035.657 kb on + strand at 1035.657 kb on + strand at 1035.659 kb on - strand at 1035.659 kb on - strand at 1035.744 kb on - strand, within MMJJ_RS05535 at 1035.744 kb on - strand, within MMJJ_RS05535 at 1035.745 kb on + strand at 1035.747 kb on + strand, within MMJJ_RS05535 at 1035.914 kb on + strand, within MMJJ_RS05535 at 1035.914 kb on + strand, within MMJJ_RS05535 at 1035.918 kb on + strand, within MMJJ_RS05535 at 1035.918 kb on + strand, within MMJJ_RS05535 at 1035.924 kb on - strand, within MMJJ_RS05535 at 1035.970 kb on - strand, within MMJJ_RS05535 at 1035.993 kb on + strand, within MMJJ_RS05535 at 1036.017 kb on + strand, within MMJJ_RS05535 at 1036.024 kb on - strand, within MMJJ_RS05535 at 1036.025 kb on + strand, within MMJJ_RS05535 at 1036.025 kb on + strand, within MMJJ_RS05535
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,033,673 - -2.0 1,033,673 - -1.4 1,033,674 + -0.9 1,033,741 - MMJJ_RS05520 0.13 +1.6 1,033,742 + MMJJ_RS05520 0.13 +3.6 1,033,742 + MMJJ_RS05520 0.13 -2.1 1,033,746 - MMJJ_RS05520 0.14 +2.5 1,033,811 + MMJJ_RS05520 0.20 -3.5 1,033,816 + MMJJ_RS05520 0.21 -1.4 1,033,816 + MMJJ_RS05520 0.21 +2.1 1,033,818 - MMJJ_RS05520 0.21 +4.7 1,033,901 + MMJJ_RS05520 0.29 -0.7 1,033,906 - MMJJ_RS05520 0.29 +0.1 1,033,906 - MMJJ_RS05520 0.29 -1.4 1,033,907 + MMJJ_RS05520 0.30 -0.7 1,033,907 + MMJJ_RS05520 0.30 -0.2 1,033,912 + MMJJ_RS05520 0.30 -0.9 1,033,945 - MMJJ_RS05520 0.33 +1.0 1,033,945 - MMJJ_RS05520 0.33 +2.3 1,033,946 + MMJJ_RS05520 0.33 +1.0 1,033,946 + MMJJ_RS05520 0.33 -1.1 1,033,946 + MMJJ_RS05520 0.33 +1.0 1,033,946 + MMJJ_RS05520 0.33 -0.7 1,033,951 - MMJJ_RS05520 0.34 +1.8 1,033,952 + MMJJ_RS05520 0.34 +1.2 1,033,972 + MMJJ_RS05520 0.36 +1.8 1,033,974 + MMJJ_RS05520 0.36 -0.2 1,033,995 - MMJJ_RS05520 0.38 -1.5 1,034,002 - MMJJ_RS05520 0.39 -1.3 1,034,002 - MMJJ_RS05520 0.39 +0.2 1,034,014 - MMJJ_RS05520 0.40 -1.2 1,034,020 - MMJJ_RS05520 0.41 +2.2 1,034,023 - MMJJ_RS05520 0.41 +1.6 1,034,024 + MMJJ_RS05520 0.41 -1.7 1,034,024 + MMJJ_RS05520 0.41 -1.4 1,034,072 - MMJJ_RS05520 0.46 +0.4 1,034,072 - MMJJ_RS05520 0.46 -1.4 1,034,073 + MMJJ_RS05520 0.46 +0.9 1,034,073 + MMJJ_RS05520 0.46 -1.2 1,034,073 + MMJJ_RS05520 0.46 +2.1 1,034,109 + MMJJ_RS05520 0.49 -0.6 1,034,109 + MMJJ_RS05520 0.49 +4.2 1,034,109 + MMJJ_RS05520 0.49 -1.6 1,034,109 + MMJJ_RS05520 0.49 -0.9 1,034,191 + MMJJ_RS05520 0.57 -2.9 1,034,278 + MMJJ_RS05520 0.66 +1.5 1,034,362 + MMJJ_RS05520 0.74 -1.2 1,034,373 - MMJJ_RS05520 0.75 +1.1 1,034,476 + MMJJ_RS05520 0.85 -0.3 1,034,487 - MMJJ_RS05520 0.86 +1.4 1,034,487 - MMJJ_RS05520 0.86 -1.7 1,034,597 + -1.1 1,034,600 + -2.4 1,034,600 + -1.8 1,034,608 - -1.9 1,034,608 - -2.3 1,034,612 + +3.8 1,034,644 - +0.7 1,034,699 + MMJJ_RS05525 0.12 +1.1 1,034,710 - MMJJ_RS05525 0.15 -2.5 1,034,745 + MMJJ_RS05525 0.22 -2.0 1,034,763 - MMJJ_RS05525 0.26 -2.3 1,034,763 - MMJJ_RS05525 0.26 -1.4 1,034,764 + MMJJ_RS05525 0.26 -3.8 1,034,768 - MMJJ_RS05525 0.27 +2.5 1,034,866 + MMJJ_RS05525 0.48 -1.5 1,034,876 + MMJJ_RS05525 0.50 -3.0 1,034,918 - MMJJ_RS05525 0.59 -2.0 1,034,919 + MMJJ_RS05525 0.59 +0.8 1,034,919 + MMJJ_RS05525 0.59 -1.0 1,034,937 + MMJJ_RS05525 0.63 -3.4 1,034,937 + MMJJ_RS05525 0.63 +1.2 1,034,939 - MMJJ_RS05525 0.63 -1.6 1,034,939 - MMJJ_RS05525 0.63 -1.2 1,034,939 - MMJJ_RS05525 0.63 +3.7 1,034,940 + MMJJ_RS05525 0.63 +3.2 1,034,940 + MMJJ_RS05525 0.63 +0.2 1,034,964 + MMJJ_RS05525 0.68 -1.6 1,035,011 + MMJJ_RS05525 0.78 -0.6 1,035,015 + MMJJ_RS05525 0.79 -1.0 1,035,015 + MMJJ_RS05525 0.79 -1.8 1,035,029 - MMJJ_RS05525 0.82 +0.1 1,035,029 - MMJJ_RS05525 0.82 -2.0 1,035,031 - MMJJ_RS05525 0.82 -0.4 1,035,045 + MMJJ_RS05525 0.85 -1.9 1,035,079 - -1.1 1,035,088 - +2.8 1,035,089 + +0.4 1,035,089 + +0.8 1,035,111 - -1.3 1,035,123 - +0.2 1,035,123 - +2.5 1,035,123 - -1.4 1,035,124 + -0.1 1,035,124 + -2.9 1,035,124 + -1.4 1,035,124 + +0.6 1,035,124 + +1.4 1,035,124 + -1.0 1,035,127 - +1.2 1,035,128 + +2.6 1,035,128 + -0.9 1,035,128 + -2.8 1,035,172 + MMJJ_RS05530 0.13 +0.5 1,035,190 + MMJJ_RS05530 0.18 -2.7 1,035,190 + MMJJ_RS05530 0.18 +2.1 1,035,390 - MMJJ_RS05530 0.76 +1.6 1,035,391 + MMJJ_RS05530 0.76 +2.0 1,035,391 + MMJJ_RS05530 0.76 +1.6 1,035,430 + MMJJ_RS05530 0.88 -0.8 1,035,506 - -0.2 1,035,520 + -0.3 1,035,538 + +1.4 1,035,548 + -1.2 1,035,550 - -3.2 1,035,571 - -0.6 1,035,618 - +2.6 1,035,618 - +0.5 1,035,619 + -1.4 1,035,619 + -0.9 1,035,619 + -2.5 1,035,624 - -0.0 1,035,625 + +1.5 1,035,625 + -2.3 1,035,625 + +1.4 1,035,625 + +2.7 1,035,656 - -0.4 1,035,657 + -2.1 1,035,657 + -2.2 1,035,659 - -2.0 1,035,659 - +0.8 1,035,744 - MMJJ_RS05535 0.11 -2.8 1,035,744 - MMJJ_RS05535 0.11 -2.9 1,035,745 + +0.4 1,035,747 + MMJJ_RS05535 0.11 -1.1 1,035,914 + MMJJ_RS05535 0.29 -2.4 1,035,914 + MMJJ_RS05535 0.29 -2.0 1,035,918 + MMJJ_RS05535 0.29 -3.6 1,035,918 + MMJJ_RS05535 0.29 -3.4 1,035,924 - MMJJ_RS05535 0.30 -1.2 1,035,970 - MMJJ_RS05535 0.34 -2.7 1,035,993 + MMJJ_RS05535 0.37 -1.9 1,036,017 + MMJJ_RS05535 0.39 -0.9 1,036,024 - MMJJ_RS05535 0.40 -1.4 1,036,025 + MMJJ_RS05535 0.40 -2.0 1,036,025 + MMJJ_RS05535 0.40 -1.7
Or see this region's nucleotide sequence