Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05405 and MMJJ_RS05410 are separated by 42 nucleotides MMJJ_RS05410 and MMJJ_RS05415 are separated by 27 nucleotides MMJJ_RS05415 and MMJJ_RS05420 are separated by 10 nucleotides
MMJJ_RS05405: MMJJ_RS05405 - DNA cytosine methyltransferase, at 1,003,602 to 1,004,669
_RS05405
MMJJ_RS05410: MMJJ_RS05410 - RecB-family nuclease, at 1,004,712 to 1,005,137
_RS05410
MMJJ_RS05415: MMJJ_RS05415 - hypothetical protein, at 1,005,165 to 1,006,298
_RS05415
MMJJ_RS05420: MMJJ_RS05420 - DNA-directed DNA polymerase II small subunit, at 1,006,309 to 1,008,066
_RS05420
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1004.181 kb on - strand, within MMJJ_RS05405 at 1004.240 kb on - strand, within MMJJ_RS05405 at 1004.276 kb on - strand, within MMJJ_RS05405 at 1004.288 kb on + strand, within MMJJ_RS05405 at 1004.312 kb on - strand, within MMJJ_RS05405 at 1004.399 kb on - strand, within MMJJ_RS05405 at 1004.425 kb on - strand, within MMJJ_RS05405 at 1004.426 kb on + strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.434 kb on + strand, within MMJJ_RS05405 at 1004.434 kb on + strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.516 kb on + strand, within MMJJ_RS05405 at 1004.579 kb on - strand at 1004.579 kb on - strand at 1004.579 kb on - strand at 1004.580 kb on + strand at 1004.589 kb on - strand at 1004.590 kb on + strand at 1004.590 kb on + strand at 1004.655 kb on - strand at 1004.656 kb on + strand at 1004.661 kb on + strand at 1004.663 kb on - strand at 1004.663 kb on - strand at 1004.667 kb on - strand at 1004.668 kb on + strand at 1004.690 kb on + strand at 1004.697 kb on + strand at 1004.800 kb on - strand, within MMJJ_RS05410 at 1004.801 kb on + strand, within MMJJ_RS05410 at 1004.804 kb on - strand, within MMJJ_RS05410 at 1004.805 kb on + strand, within MMJJ_RS05410 at 1004.863 kb on - strand, within MMJJ_RS05410 at 1004.863 kb on - strand, within MMJJ_RS05410 at 1004.864 kb on + strand, within MMJJ_RS05410 at 1004.935 kb on - strand, within MMJJ_RS05410 at 1004.936 kb on + strand, within MMJJ_RS05410 at 1004.936 kb on + strand, within MMJJ_RS05410 at 1004.936 kb on + strand, within MMJJ_RS05410 at 1004.939 kb on + strand, within MMJJ_RS05410 at 1004.941 kb on - strand, within MMJJ_RS05410 at 1004.981 kb on - strand, within MMJJ_RS05410 at 1004.988 kb on + strand, within MMJJ_RS05410 at 1004.988 kb on + strand, within MMJJ_RS05410 at 1005.071 kb on - strand, within MMJJ_RS05410 at 1005.072 kb on + strand, within MMJJ_RS05410 at 1005.072 kb on + strand, within MMJJ_RS05410 at 1005.076 kb on - strand, within MMJJ_RS05410 at 1005.084 kb on + strand, within MMJJ_RS05410 at 1005.136 kb on + strand at 1005.140 kb on - strand at 1005.140 kb on - strand at 1005.141 kb on + strand at 1005.143 kb on + strand at 1005.381 kb on - strand, within MMJJ_RS05415 at 1005.435 kb on + strand at 1005.584 kb on + strand, within MMJJ_RS05415 at 1006.051 kb on - strand, within MMJJ_RS05415 at 1006.095 kb on + strand, within MMJJ_RS05415 at 1006.142 kb on + strand, within MMJJ_RS05415 at 1006.258 kb on - strand at 1006.537 kb on - strand, within MMJJ_RS05420 at 1006.537 kb on - strand, within MMJJ_RS05420 at 1006.875 kb on + strand at 1007.061 kb on + strand, within MMJJ_RS05420
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 1,004,181 - MMJJ_RS05405 0.54 -3.9 1,004,240 - MMJJ_RS05405 0.60 -2.6 1,004,276 - MMJJ_RS05405 0.63 -1.2 1,004,288 + MMJJ_RS05405 0.64 -1.4 1,004,312 - MMJJ_RS05405 0.66 -0.4 1,004,399 - MMJJ_RS05405 0.75 +2.0 1,004,425 - MMJJ_RS05405 0.77 +3.6 1,004,426 + MMJJ_RS05405 0.77 -1.0 1,004,433 - MMJJ_RS05405 0.78 +0.4 1,004,433 - MMJJ_RS05405 0.78 -2.7 1,004,433 - MMJJ_RS05405 0.78 +1.1 1,004,433 - MMJJ_RS05405 0.78 +2.6 1,004,434 + MMJJ_RS05405 0.78 +0.4 1,004,434 + MMJJ_RS05405 0.78 +0.9 1,004,489 - MMJJ_RS05405 0.83 +0.2 1,004,489 - MMJJ_RS05405 0.83 +2.5 1,004,489 - MMJJ_RS05405 0.83 -1.9 1,004,489 - MMJJ_RS05405 0.83 -2.0 1,004,516 + MMJJ_RS05405 0.86 +1.0 1,004,579 - -0.6 1,004,579 - +1.3 1,004,579 - -0.4 1,004,580 + -2.2 1,004,589 - +2.1 1,004,590 + +1.0 1,004,590 + -2.0 1,004,655 - +0.4 1,004,656 + +2.5 1,004,661 + -0.3 1,004,663 - -2.2 1,004,663 - -1.4 1,004,667 - +0.4 1,004,668 + -0.1 1,004,690 + -0.7 1,004,697 + +1.4 1,004,800 - MMJJ_RS05410 0.21 +0.3 1,004,801 + MMJJ_RS05410 0.21 -2.2 1,004,804 - MMJJ_RS05410 0.22 +0.6 1,004,805 + MMJJ_RS05410 0.22 +2.0 1,004,863 - MMJJ_RS05410 0.35 -0.1 1,004,863 - MMJJ_RS05410 0.35 +1.4 1,004,864 + MMJJ_RS05410 0.36 +3.8 1,004,935 - MMJJ_RS05410 0.52 +1.3 1,004,936 + MMJJ_RS05410 0.53 +2.8 1,004,936 + MMJJ_RS05410 0.53 -1.4 1,004,936 + MMJJ_RS05410 0.53 +1.7 1,004,939 + MMJJ_RS05410 0.53 +1.3 1,004,941 - MMJJ_RS05410 0.54 +2.5 1,004,981 - MMJJ_RS05410 0.63 -3.4 1,004,988 + MMJJ_RS05410 0.65 +1.5 1,004,988 + MMJJ_RS05410 0.65 -0.0 1,005,071 - MMJJ_RS05410 0.84 +1.4 1,005,072 + MMJJ_RS05410 0.85 -0.3 1,005,072 + MMJJ_RS05410 0.85 -0.3 1,005,076 - MMJJ_RS05410 0.85 -0.1 1,005,084 + MMJJ_RS05410 0.87 -1.3 1,005,136 + -0.4 1,005,140 - -1.2 1,005,140 - -2.7 1,005,141 + +2.6 1,005,143 + -2.0 1,005,381 - MMJJ_RS05415 0.19 -0.6 1,005,435 + -0.2 1,005,584 + MMJJ_RS05415 0.37 +1.4 1,006,051 - MMJJ_RS05415 0.78 -0.6 1,006,095 + MMJJ_RS05415 0.82 -0.9 1,006,142 + MMJJ_RS05415 0.86 -0.9 1,006,258 - -1.1 1,006,537 - MMJJ_RS05420 0.13 +1.4 1,006,537 - MMJJ_RS05420 0.13 -1.4 1,006,875 + +0.4 1,007,061 + MMJJ_RS05420 0.43 -0.6
Or see this region's nucleotide sequence